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The map label for this gene is pdhC [H]

Identifier: 50364856

GI number: 50364856

Start: 55798

End: 57066

Strand: Direct

Name: pdhC [H]

Synonym: Mfl041

Alternate gene names: 50364856

Gene position: 55798-57066 (Clockwise)

Preceding gene: 50364855

Following gene: 50364857

Centisome position: 7.03

GC content: 32.15

Gene sequence:

>1269_bases
ATGTTTAAAGTAAAATTTGCCGACATTGGAGAAGGGCTTACTGAAGGAAAAGTAGCAGAAGTTCTTGTTAAATTAGGGCA
AGAAATAAAAGAAGGAGATGCTTTATTTTTTGTTGAGACAGATAAAGTTAACTCTGAAATTCCAGCACCAGTTGGTGGAA
AGATTGCAAATATTTTAATTTCTCAAGGACAAGAAATTAAAGTTGGAGATGTTGTTATTGAAATTGATGATGGTTCTTCA
GCGGCTGAAGTAACACCTGTAGCAGAAATTAAAACTAAGAATGAACCAATTGAAGAAAATGCTTCAGTTGTAGGGTCAAC
ACCTGTATCAAATGATGTTATTGCTTCAAGAGCAACAACAAATGCAGCAGCAGAAATTTCAAATAGTGGTGTTAAAGCAA
CACCTTTAGCTAGAAAAATTGCAGCTGATAAAAAAATTGATTTATCAACAATTAAAGGTACTGGACCTCATGGAAGAATT
TTAGTTTCTGATTTAGATTCAGCACCAGTTGCCGTTCCAAATACAAACTCAGCAACTAAAACAGTTGTTAAATCTGTTGA
TGTAGATGCACCATTATCATGAGATTCAATCCCAATGAATGGAATTAGAAAAGCAACTGTTAAAGCAATGGTTAAATCTC
AATCAGAAAATGCAGCATTTACAGGAATGAAAAATATTAACATAACTCCAACATATGATATGCGTGCAATGTTAAAAGAT
GGATGTGAATCAAAAGGAATTAAATTAACTTACTTAGCATTCATCGTAAAAGCAGCTGCTAAAGTATTGGAAGAAATGCC
TAATATTAATGTTCGTATAGATGCTGAAAACAATGCAATCTTACAAGTACATAATATTAATATTGGTATCGCAGTTGATA
CTGAAAAAGGTTTAATGGTTCCTGTTATTAAAGGAGCAAACCATTTAACAATATTTGAAATCGCTAATAAAATTGGTGAA
TTAGCTAAAAAAGCTAGAGATGGTAAATTAGCAATGACTGAAATGAAAGATGCAACTTTTACAGTTTCAAACTTTGGATC
AGTTGGATTAGATTATGCCACACCAATTATTAATTCACCTGAGTCAGCAATCTTAGGAGTAGGTACAATGACAAAAACAC
CTATTTTTGTAAAAGATGAAATTAAAGCAGGTTGAATCATGCCATTCTCAATGACATGTGATCATAGAATAATTGATGGT
GGAGATGCCGGAAGATTCTTAATGAAAATAGAAAATTATTTAAGTAATCCAGCATTACTATTAATGTAA

Upstream 100 bases:

>100_bases
ACAGAGGGGAAGGATTCCACCAAGTGAACCCACAGAAAGTTGTAGAAGCAATTAAAAAAGTGCTTGACTACAAATTTTAG
TAGAGAGGAATTAGAATAAT

Downstream 100 bases:

>100_bases
AAGAGGAGAAGCAAGATGTTTAAAGTAAAATTTGCCGACATTGGAGAAGGGCTTACTGAAGGAAAAGTAGCAGAAGTTCT
TGTTAAATTAGGGCAAGAAA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MFKVKFADIGEGLTEGKVAEVLVKLGQEIKEGDALFFVETDKVNSEIPAPVGGKIANILISQGQEIKVGDVVIEIDDGSS
AAEVTPVAEIKTKNEPIEENASVVGSTPVSNDVIASRATTNAAAEISNSGVKATPLARKIAADKKIDLSTIKGTGPHGRI
LVSDLDSAPVAVPNTNSATKTVVKSVDVDAPLSWDSIPMNGIRKATVKAMVKSQSENAAFTGMKNINITPTYDMRAMLKD
GCESKGIKLTYLAFIVKAAAKVLEEMPNINVRIDAENNAILQVHNINIGIAVDTEKGLMVPVIKGANHLTIFEIANKIGE
LAKKARDGKLAMTEMKDATFTVSNFGSVGLDYATPIINSPESAILGVGTMTKTPIFVKDEIKAGWIMPFSMTCDHRIIDG
GDAGRFLMKIENYLSNPALLLM

Sequences:

>Translated_422_residues
MFKVKFADIGEGLTEGKVAEVLVKLGQEIKEGDALFFVETDKVNSEIPAPVGGKIANILISQGQEIKVGDVVIEIDDGSS
AAEVTPVAEIKTKNEPIEENASVVGSTPVSNDVIASRATTNAAAEISNSGVKATPLARKIAADKKIDLSTIKGTGPHGRI
LVSDLDSAPVAVPNTNSATKTVVKSVDVDAPLS*DSIPMNGIRKATVKAMVKSQSENAAFTGMKNINITPTYDMRAMLKD
GCESKGIKLTYLAFIVKAAAKVLEEMPNINVRIDAENNAILQVHNINIGIAVDTEKGLMVPVIKGANHLTIFEIANKIGE
LAKKARDGKLAMTEMKDATFTVSNFGSVGLDYATPIINSPESAILGVGTMTKTPIFVKDEIKAG*IMPFSMTCDHRIIDG
GDAGRFLMKIENYLSNPALLLM
>Mature_422_residues
MFKVKFADIGEGLTEGKVAEVLVKLGQEIKEGDALFFVETDKVNSEIPAPVGGKIANILISQGQEIKVGDVVIEIDDGSS
AAEVTPVAEIKTKNEPIEENASVVGSTPVSNDVIASRATTNAAAEISNSGVKATPLARKIAADKKIDLSTIKGTGPHGRI
LVSDLDSAPVAVPNTNSATKTVVKSVDVDAPLS*DSIPMNGIRKATVKAMVKSQSENAAFTGMKNINITPTYDMRAMLKD
GCESKGIKLTYLAFIVKAAAKVLEEMPNINVRIDAENNAILQVHNINIGIAVDTEKGLMVPVIKGANHLTIFEIANKIGE
LAKKARDGKLAMTEMKDATFTVSNFGSVGLDYATPIINSPESAILGVGTMTKTPIFVKDEIKAG*IMPFSMTCDHRIIDG
GDAGRFLMKIENYLSNPALLLM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=439, Percent_Identity=31.4350797266515, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI31711992, Length=426, Percent_Identity=28.8732394366197, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=35.5555555555556, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI203098753, Length=465, Percent_Identity=26.8817204301075, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI203098816, Length=466, Percent_Identity=26.8240343347639, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.888198757764, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1786946, Length=418, Percent_Identity=29.4258373205742, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1786305, Length=426, Percent_Identity=30.2816901408451, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=431, Percent_Identity=30.6264501160093, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17560088, Length=431, Percent_Identity=32.4825986078886, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=418, Percent_Identity=31.5789473684211, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17538894, Length=227, Percent_Identity=30.3964757709251, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=415, Percent_Identity=30.8433734939759, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6324258, Length=443, Percent_Identity=25.2821670428894, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI18859875, Length=438, Percent_Identity=31.7351598173516, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24582497, Length=427, Percent_Identity=25.9953161592506, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI20129315, Length=437, Percent_Identity=25.629290617849, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=35.3982300884956, Blast_Score=128, Evalue=6e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 44456; Mature: 44456

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKVKFADIGEGLTEGKVAEVLVKLGQEIKEGDALFFVETDKVNSEIPAPVGGKIANILI
CEEEEEHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHH
SQGQEIKVGDVVIEIDDGSSAAEVTPVAEIKTKNEPIEENASVVGSTPVSNDVIASRATT
CCCCEEEECEEEEEECCCCCCCEECCHHEECCCCCCCCCCCCEEECCCCCCCCEECCCCC
NAAAEISNSGVKATPLARKIAADKKIDLSTIKGTGPHGRILVSDLDSAPVAVPNTNSATK
CHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCEECCCCCCHHH
TVVKSVDVDAPLSDSIPMNGIRKATVKAMVKSQSENAAFTGMKNINITPTYDMRAMLKDG
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHCC
CESKGIKLTYLAFIVKAAAKVLEEMPNINVRIDAENNAILQVHNINIGIAVDTEKGLMVP
CCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEEEEEEEECCCCEEEE
VIKGANHLTIFEIANKIGELAKKARDGKLAMTEMKDATFTVSNFGSVGLDYATPIINSPE
EECCCCCEEHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCC
SAILGVGTMTKTPIFVKDEIKAGIMPFSMTCDHRIIDGGDAGRFLMKIENYLSNPALLLM
CCEEECCCCCCCCEEEECCCCCCCEEEEECCCCEEECCCCCCHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure
MFKVKFADIGEGLTEGKVAEVLVKLGQEIKEGDALFFVETDKVNSEIPAPVGGKIANILI
CEEEEEHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHH
SQGQEIKVGDVVIEIDDGSSAAEVTPVAEIKTKNEPIEENASVVGSTPVSNDVIASRATT
CCCCEEEECEEEEEECCCCCCCEECCHHEECCCCCCCCCCCCEEECCCCCCCCEECCCCC
NAAAEISNSGVKATPLARKIAADKKIDLSTIKGTGPHGRILVSDLDSAPVAVPNTNSATK
CHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCEECCCCCCHHH
TVVKSVDVDAPLSDSIPMNGIRKATVKAMVKSQSENAAFTGMKNINITPTYDMRAMLKDG
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHCC
CESKGIKLTYLAFIVKAAAKVLEEMPNINVRIDAENNAILQVHNINIGIAVDTEKGLMVP
CCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEEEEEEEECCCCEEEE
VIKGANHLTIFEIANKIGELAKKARDGKLAMTEMKDATFTVSNFGSVGLDYATPIINSPE
EECCCCCEEHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCC
SAILGVGTMTKTPIFVKDEIKAGIMPFSMTCDHRIIDGGDAGRFLMKIENYLSNPALLLM
CCEEECCCCCCCCEEEECCCCCCCEEEEECCCCEEECCCCCCHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8844861 [H]