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The map label for this gene is pdhD [H]

Identifier: 50364857

GI number: 50364857

Start: 57082

End: 58890

Strand: Direct

Name: pdhD [H]

Synonym: Mfl042

Alternate gene names: 50364857

Gene position: 57082-58890 (Clockwise)

Preceding gene: 50364856

Following gene: 50364858

Centisome position: 7.2

GC content: 32.39

Gene sequence:

>1809_bases
ATGTTTAAAGTAAAATTTGCCGACATTGGAGAAGGGCTTACTGAAGGAAAAGTAGCAGAAGTTCTTGTTAAATTAGGGCA
AGAAATAAAAGAAGGAGATGCTTTATTTTTTGTTGAGACAGATAAAGTTAACTCTGAAATTCCAGCACCAGTTGGTGGAA
AGATTGCCAAAGTTTTAATTTCTGAAGGACAAGAAATTAAAGTTGGAGATGTTGTTATTGAAATTGATGATGGAAGTGCT
ACTGTTGAGGCAGCACCAGCTGCAGAGGAAGAAAATGCTTCAGTTGTTGGTTCAACTCCTGTATCAAATGATTTAATTCC
AAGTAGAGGGCCAGCACCAACTCAAAATGTTGCTGCTCAACCAACTCCAGCACCAGTTGCAACAAAACATACAGATATTG
AAGAAAGCTTTGATGTTATTGTTGTAGGAGCTGGTATTGGTGGTTATGTTTCAGCTATTAAAACAGCTCAACTAGGATTA
AAAACATTAATTATTGAAAAACAATATTATGGTGGTGTTTGTTTAAATGTTGGATGTATACCAACTAAATCATTATTAAG
AACAGCTAAAGTTTTTGAAGATATAGTTCACAAAGCTGCTAACTTAGGTATTGATATGAAAACAAAAGATGAACCTAGCA
TAAACTGAAATAAAGCACTTGAGCGTAAAGACGGTGTTGTTAATAAATTAACTGGTGGAGTTAAAGTATTATTAACTAAA
AATGGTGTAAAACAAATTATTGGTGAAGCATCAGCTTTAGATAAAAATACAATCTCTGTAAATGGCAAAAAATACCACTG
TGACAATTTAATAATTGCTAGTGGTTCAGTTCCAAACGAATTACCATTACCAGGATTTGCAGAAGGAAGAGAAAGTGGAT
TCTTAATCGATTCAACAAAAATTCTTTCATTACCAAAAATACCAAAAACTTTAACTGTAATTGGTGGTGGTGTTATCGGA
ATAGAATTTGGTTGTTTATTTGCAGCTTTAGGAACAAAAGTTACTGTTATTGAAGGTGCACCAAAAATTCTTCCTATGTT
AGATCAAGATGTAACAGCATTAATGACAAAAACATTAAAAGAAAAATACAAAATTGAAATATTTACTAATGCTAAAGTTA
AAGAAGTTAAAGGTAAATCAGTTGTATTTGAAATTGATGGTAAAGAACAAACTGTTAAATCAGATTACTGTTTAGAATCA
ATTGGAAGAAAAACAGTTACTAAAGGATTTGATGGAATAGGTCTTGAATTATCAGAACGTAAATCAATCATTGCAAATGA
TTATGGTGAAACAAACTTAGAAGGTGTATATGCAATTGGGGACGTAACAAGTAAAATTATGTTAGCTCACGTTGCTTCAC
ACGCTGGTATTGTTACAGCAAACAGAATTGCTTTAAAAGCAAATAAACCAGATGCACATGATATCAAAATGGACTATTCA
AAAATACCAAGCTGTATTTACTCACACCCTGAAATTGCTATGATTGGTAAAACTGAACAACAATTAAAAGAAGAGGGAGT
TGAATATAAAACATTTAAATTCCCATTTGCAGCTATTGGTAAAGCATTAGCTGATGATGACACAACAGGATTTGTAAAAA
TTATTTGTGAACCAAAATATAAAACTTTATTAGGTGCACATATTATCGGAAATAGAGCAACAGACATGATCTCAGAATTC
ACAACTTTAATTGAATGTGAAGGAACTATTACAGAATTGGCAAGAGCTATCCATCCTCATCCAACTATGAGTGAAGCTAT
TGGTGAAGCAGCTGAAGCATTAGAATCAGGAAAATCATTAAACCTTTAA

Upstream 100 bases:

>100_bases
TAGAATAATTGATGGTGGAGATGCCGGAAGATTCTTAATGAAAATAGAAAATTATTTAAGTAATCCAGCATTACTATTAA
TGTAAAAGAGGAGAAGCAAG

Downstream 100 bases:

>100_bases
TATGTATTCAGTAGAACAAATCAAAAAAATTCTAGTAGACTCAGATCATAAAAAAACCATAGTTTTACCCGAAGGTGAAG
AACCTAAAATTCAAGAAGTA

Product: dihydrolipate dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MFKVKFADIGEGLTEGKVAEVLVKLGQEIKEGDALFFVETDKVNSEIPAPVGGKIAKVLISEGQEIKVGDVVIEIDDGSA
TVEAAPAAEEENASVVGSTPVSNDLIPSRGPAPTQNVAAQPTPAPVATKHTDIEESFDVIVVGAGIGGYVSAIKTAQLGL
KTLIIEKQYYGGVCLNVGCIPTKSLLRTAKVFEDIVHKAANLGIDMKTKDEPSINWNKALERKDGVVNKLTGGVKVLLTK
NGVKQIIGEASALDKNTISVNGKKYHCDNLIIASGSVPNELPLPGFAEGRESGFLIDSTKILSLPKIPKTLTVIGGGVIG
IEFGCLFAALGTKVTVIEGAPKILPMLDQDVTALMTKTLKEKYKIEIFTNAKVKEVKGKSVVFEIDGKEQTVKSDYCLES
IGRKTVTKGFDGIGLELSERKSIIANDYGETNLEGVYAIGDVTSKIMLAHVASHAGIVTANRIALKANKPDAHDIKMDYS
KIPSCIYSHPEIAMIGKTEQQLKEEGVEYKTFKFPFAAIGKALADDDTTGFVKIICEPKYKTLLGAHIIGNRATDMISEF
TTLIECEGTITELARAIHPHPTMSEAIGEAAEALESGKSLNL

Sequences:

>Translated_602_residues
MFKVKFADIGEGLTEGKVAEVLVKLGQEIKEGDALFFVETDKVNSEIPAPVGGKIAKVLISEGQEIKVGDVVIEIDDGSA
TVEAAPAAEEENASVVGSTPVSNDLIPSRGPAPTQNVAAQPTPAPVATKHTDIEESFDVIVVGAGIGGYVSAIKTAQLGL
KTLIIEKQYYGGVCLNVGCIPTKSLLRTAKVFEDIVHKAANLGIDMKTKDEPSIN*NKALERKDGVVNKLTGGVKVLLTK
NGVKQIIGEASALDKNTISVNGKKYHCDNLIIASGSVPNELPLPGFAEGRESGFLIDSTKILSLPKIPKTLTVIGGGVIG
IEFGCLFAALGTKVTVIEGAPKILPMLDQDVTALMTKTLKEKYKIEIFTNAKVKEVKGKSVVFEIDGKEQTVKSDYCLES
IGRKTVTKGFDGIGLELSERKSIIANDYGETNLEGVYAIGDVTSKIMLAHVASHAGIVTANRIALKANKPDAHDIKMDYS
KIPSCIYSHPEIAMIGKTEQQLKEEGVEYKTFKFPFAAIGKALADDDTTGFVKIICEPKYKTLLGAHIIGNRATDMISEF
TTLIECEGTITELARAIHPHPTMSEAIGEAAEALESGKSLNL
>Mature_602_residues
MFKVKFADIGEGLTEGKVAEVLVKLGQEIKEGDALFFVETDKVNSEIPAPVGGKIAKVLISEGQEIKVGDVVIEIDDGSA
TVEAAPAAEEENASVVGSTPVSNDLIPSRGPAPTQNVAAQPTPAPVATKHTDIEESFDVIVVGAGIGGYVSAIKTAQLGL
KTLIIEKQYYGGVCLNVGCIPTKSLLRTAKVFEDIVHKAANLGIDMKTKDEPSIN*NKALERKDGVVNKLTGGVKVLLTK
NGVKQIIGEASALDKNTISVNGKKYHCDNLIIASGSVPNELPLPGFAEGRESGFLIDSTKILSLPKIPKTLTVIGGGVIG
IEFGCLFAALGTKVTVIEGAPKILPMLDQDVTALMTKTLKEKYKIEIFTNAKVKEVKGKSVVFEIDGKEQTVKSDYCLES
IGRKTVTKGFDGIGLELSERKSIIANDYGETNLEGVYAIGDVTSKIMLAHVASHAGIVTANRIALKANKPDAHDIKMDYS
KIPSCIYSHPEIAMIGKTEQQLKEEGVEYKTFKFPFAAIGKALADDDTTGFVKIICEPKYKTLLGAHIIGNRATDMISEF
TTLIECEGTITELARAIHPHPTMSEAIGEAAEALESGKSLNL

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=507, Percent_Identity=37.8698224852071, Blast_Score=289, Evalue=6e-78,
Organism=Homo sapiens, GI50301238, Length=471, Percent_Identity=30.9978768577495, Blast_Score=194, Evalue=3e-49,
Organism=Homo sapiens, GI33519430, Length=483, Percent_Identity=25.6728778467909, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI33519428, Length=483, Percent_Identity=25.6728778467909, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI33519426, Length=483, Percent_Identity=25.6728778467909, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI148277065, Length=483, Percent_Identity=25.6728778467909, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI148277071, Length=481, Percent_Identity=25.7796257796258, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI22035672, Length=473, Percent_Identity=26.0042283298097, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI291045266, Length=507, Percent_Identity=26.4299802761341, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI291045268, Length=501, Percent_Identity=24.5508982035928, Blast_Score=89, Evalue=2e-17,
Organism=Escherichia coli, GI1786307, Length=467, Percent_Identity=35.9743040685225, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI1789915, Length=444, Percent_Identity=30.1801801801802, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=26.4642082429501, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=28.4796573875803, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32565766, Length=490, Percent_Identity=37.5510204081633, Blast_Score=301, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI71983419, Length=458, Percent_Identity=27.0742358078603, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=458, Percent_Identity=27.0742358078603, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17557007, Length=484, Percent_Identity=24.1735537190083, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71982272, Length=492, Percent_Identity=26.219512195122, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=483, Percent_Identity=39.1304347826087, Blast_Score=273, Evalue=5e-74,
Organism=Saccharomyces cerevisiae, GI6325166, Length=480, Percent_Identity=27.9166666666667, Blast_Score=164, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6325240, Length=503, Percent_Identity=26.2425447316103, Blast_Score=146, Evalue=8e-36,
Organism=Drosophila melanogaster, GI21358499, Length=481, Percent_Identity=36.1746361746362, Blast_Score=278, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24640549, Length=496, Percent_Identity=26.008064516129, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24640551, Length=496, Percent_Identity=26.008064516129, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24640553, Length=496, Percent_Identity=26.008064516129, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI17737741, Length=500, Percent_Identity=23.6, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 63941; Mature: 63941

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKVKFADIGEGLTEGKVAEVLVKLGQEIKEGDALFFVETDKVNSEIPAPVGGKIAKVLI
CEEEEEHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHH
SEGQEIKVGDVVIEIDDGSATVEAAPAAEEENASVVGSTPVSNDLIPSRGPAPTQNVAAQ
CCCCEEEECEEEEEECCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCC
PTPAPVATKHTDIEESFDVIVVGAGIGGYVSAIKTAQLGLKTLIIEKQYYGGVCLNVGCI
CCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCEEEEEEEECCCCCEEEEECCC
PTKSLLRTAKVFEDIVHKAANLGIDMKTKDEPSINNKALERKDGVVNKLTGGVKVLLTKN
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCHHHHHCCCEEEEEECC
GVKQIIGEASALDKNTISVNGKKYHCDNLIIASGSVPNELPLPGFAEGRESGFLIDSTKI
CHHHHHCCHHHCCCCEEEECCCEEECCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCEE
LSLPKIPKTLTVIGGGVIGIEFGCLFAALGTKVTVIEGAPKILPMLDQDVTALMTKTLKE
EECCCCCCEEEEECCCEEEHHHHHHHHHHCCEEEEECCCCHHHHHHCHHHHHHHHHHHHH
KYKIEIFTNAKVKEVKGKSVVFEIDGKEQTVKSDYCLESIGRKTVTKGFDGIGLELSERK
CEEEEEEECCEEEECCCCEEEEEECCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCHHCCC
SIIANDYGETNLEGVYAIGDVTSKIMLAHVASHAGIVTANRIALKANKPDAHDIKMDYSK
CHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCEEECHHH
IPSCIYSHPEIAMIGKTEQQLKEEGVEYKTFKFPFAAIGKALADDDTTGFVKIICEPKYK
CCHHHCCCCCEEEECCCHHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCEEEEEECCCHH
TLLGAHIIGNRATDMISEFTTLIECEGTITELARAIHPHPTMSEAIGEAAEALESGKSLN
HHHHHHHHCCHHHHHHHHHHHHEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
L
C
>Mature Secondary Structure
MFKVKFADIGEGLTEGKVAEVLVKLGQEIKEGDALFFVETDKVNSEIPAPVGGKIAKVLI
CEEEEEHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHH
SEGQEIKVGDVVIEIDDGSATVEAAPAAEEENASVVGSTPVSNDLIPSRGPAPTQNVAAQ
CCCCEEEECEEEEEECCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCC
PTPAPVATKHTDIEESFDVIVVGAGIGGYVSAIKTAQLGLKTLIIEKQYYGGVCLNVGCI
CCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCEEEEEEEECCCCCEEEEECCC
PTKSLLRTAKVFEDIVHKAANLGIDMKTKDEPSINNKALERKDGVVNKLTGGVKVLLTKN
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCHHHHHCCCEEEEEECC
GVKQIIGEASALDKNTISVNGKKYHCDNLIIASGSVPNELPLPGFAEGRESGFLIDSTKI
CHHHHHCCHHHCCCCEEEECCCEEECCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCEE
LSLPKIPKTLTVIGGGVIGIEFGCLFAALGTKVTVIEGAPKILPMLDQDVTALMTKTLKE
EECCCCCCEEEEECCCEEEHHHHHHHHHHCCEEEEECCCCHHHHHHCHHHHHHHHHHHHH
KYKIEIFTNAKVKEVKGKSVVFEIDGKEQTVKSDYCLESIGRKTVTKGFDGIGLELSERK
CEEEEEEECCEEEECCCCEEEEEECCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCHHCCC
SIIANDYGETNLEGVYAIGDVTSKIMLAHVASHAGIVTANRIALKANKPDAHDIKMDYSK
CHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCEEECHHH
IPSCIYSHPEIAMIGKTEQQLKEEGVEYKTFKFPFAAIGKALADDDTTGFVKIICEPKYK
CCHHHCCCCCEEEECCCHHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCEEEEEECCCHH
TLLGAHIIGNRATDMISEFTTLIECEGTITELARAIHPHPTMSEAIGEAAEALESGKSLN
HHHHHHHHCCHHHHHHHHHHHHEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]