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The map label for this gene is emrA [H]

Identifier: 50086617

GI number: 50086617

Start: 3592533

End: 3593609

Strand: Reverse

Name: emrA [H]

Synonym: ACIAD3679

Alternate gene names: 50086617

Gene position: 3593609-3592533 (Counterclockwise)

Preceding gene: 50086618

Following gene: 50086616

Centisome position: 99.86

GC content: 45.31

Gene sequence:

>1077_bases
ATGGATGAGCTGTCTCAAACGGATGATCGCCAAGAACCCAATTATACGCGTCGCAAAAAAGTGGCCTGGATTGTTCTTAT
ACTTCTGTTTTTAGGGATTTTACTGGGAATTTTATATTACTGGTTCGTTTATCGTTTTTATGAATCTACTGAAAATGCCT
ATGTTAAGTCAGATTTAACCTGGATTATGCCCAAAGTGTCAGGTGAAGTGATTGAGTTACTGGCCAAAGAAAATCAATAT
GTCGAAAAAGATCAACCGCTGGTAATACTCGATAATCGGGATCTACAGGCACGTGCAGATCAGGCCAAAGCCATGCTGGC
ACTCAAGCAAGCAGGTTTATCGGTGCAGTCGGAAAATGAGAAATCGGCACAGTCTACGATTCTGGAAGCTCAAAGCGCTC
GTGATGCAGCACAAGCCGATGTCAGTCGTCTTAGTTCAGATTATCGCCGCTATCAGCAATTGCTGAAAGAGGGCGTGATT
ACGCGACAACGCTTTGAGGCTACTCAAGCTGAATATCTCAGTGCACAGGCACAACTTAACAAAGCACAAGCGGCAATTCA
GGCAGCACAAGCCCAGTTTTCGGGTGTACAAGCAGGTCGCGCACAGCTCTATGCCGATCTTGATAACGCCAAAGCAACTG
TCAAACTGTACGATGTCGATTTAGGTGCATCTGAGATTCAGGCACCTGTTGCTGGAAAAATTGGAAGCTTGGCAGTCAGA
TTGGGTTCCAGAGTCTCACCGCAAACGCGTCTTATGGCGATTATTCCCAAAAATAGTCTCTATGTGGAAGCCAATTTCAA
AGAAACCCAGATTGAAAAAATGCGTGTTGGCCAGACGGTAAGTTTGGTGCTTGATGCGTATCCGAGTCTTACCTATCGTG
GCAAAGTCGAAAGTTTTGCACCTGCATCAGGCGCAACATTCTCGATGATGCCGCCAGATAATGCGACGGGCAACTTCAAC
AAGGTGGTACAACGTATCCCAGTTCGTATCTCAATCCTTCCCCATCCTCAAATTGATTTGATTAAACCAGGGATGTCGGT
CGAGGCAAAAGTTGATTTAAGAAGTCAATCAAGCTGA

Upstream 100 bases:

>100_bases
CATCTGACTGGGTCAACCTCAGGGGTCAATATTGAATAAATCCTGATGTTTCAATGCATAAAAACAAGTAATTAAATTAA
GTGTTAAAAGGTCGGACACA

Downstream 100 bases:

>100_bases
CGACATACAGCATAGGAGGGATGAACTGAGATGACTATGTCTTCCGCTTCCATAGAATGGCAATTTTCTCCGCGTGTTGC
CTGGCTGATCTTTATTGCCA

Product: multidrug resistance efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MDELSQTDDRQEPNYTRRKKVAWIVLILLFLGILLGILYYWFVYRFYESTENAYVKSDLTWIMPKVSGEVIELLAKENQY
VEKDQPLVILDNRDLQARADQAKAMLALKQAGLSVQSENEKSAQSTILEAQSARDAAQADVSRLSSDYRRYQQLLKEGVI
TRQRFEATQAEYLSAQAQLNKAQAAIQAAQAQFSGVQAGRAQLYADLDNAKATVKLYDVDLGASEIQAPVAGKIGSLAVR
LGSRVSPQTRLMAIIPKNSLYVEANFKETQIEKMRVGQTVSLVLDAYPSLTYRGKVESFAPASGATFSMMPPDNATGNFN
KVVQRIPVRISILPHPQIDLIKPGMSVEAKVDLRSQSS

Sequences:

>Translated_358_residues
MDELSQTDDRQEPNYTRRKKVAWIVLILLFLGILLGILYYWFVYRFYESTENAYVKSDLTWIMPKVSGEVIELLAKENQY
VEKDQPLVILDNRDLQARADQAKAMLALKQAGLSVQSENEKSAQSTILEAQSARDAAQADVSRLSSDYRRYQQLLKEGVI
TRQRFEATQAEYLSAQAQLNKAQAAIQAAQAQFSGVQAGRAQLYADLDNAKATVKLYDVDLGASEIQAPVAGKIGSLAVR
LGSRVSPQTRLMAIIPKNSLYVEANFKETQIEKMRVGQTVSLVLDAYPSLTYRGKVESFAPASGATFSMMPPDNATGNFN
KVVQRIPVRISILPHPQIDLIKPGMSVEAKVDLRSQSS
>Mature_358_residues
MDELSQTDDRQEPNYTRRKKVAWIVLILLFLGILLGILYYWFVYRFYESTENAYVKSDLTWIMPKVSGEVIELLAKENQY
VEKDQPLVILDNRDLQARADQAKAMLALKQAGLSVQSENEKSAQSTILEAQSARDAAQADVSRLSSDYRRYQQLLKEGVI
TRQRFEATQAEYLSAQAQLNKAQAAIQAAQAQFSGVQAGRAQLYADLDNAKATVKLYDVDLGASEIQAPVAGKIGSLAVR
LGSRVSPQTRLMAIIPKNSLYVEANFKETQIEKMRVGQTVSLVLDAYPSLTYRGKVESFAPASGATFSMMPPDNATGNFN
KVVQRIPVRISILPHPQIDLIKPGMSVEAKVDLRSQSS

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=305, Percent_Identity=35.0819672131148, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788711, Length=350, Percent_Identity=32, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1790519, Length=316, Percent_Identity=29.1139240506329, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI87081951, Length=307, Percent_Identity=23.4527687296417, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790024, Length=249, Percent_Identity=28.1124497991968, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789900, Length=300, Percent_Identity=23.3333333333333, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790012, Length=246, Percent_Identity=23.9837398373984, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39815; Mature: 39815

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDELSQTDDRQEPNYTRRKKVAWIVLILLFLGILLGILYYWFVYRFYESTENAYVKSDLT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCE
WIMPKVSGEVIELLAKENQYVEKDQPLVILDNRDLQARADQAKAMLALKQAGLSVQSENE
EEECHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
KSAQSTILEAQSARDAAQADVSRLSSDYRRYQQLLKEGVITRQRFEATQAEYLSAQAQLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAQAAIQAAQAQFSGVQAGRAQLYADLDNAKATVKLYDVDLGASEIQAPVAGKIGSLAVR
HHHHHHHHHHHHHHCCHHCHHHEEEECCCCEEEEEEEEECCCHHHHCCCCCCCHHHHHHH
LGSRVSPQTRLMAIIPKNSLYVEANFKETQIEKMRVGQTVSLVLDAYPSLTYRGKVESFA
HCCCCCCCCEEEEEEECCCEEEEECCCHHHHHHHHCCCHHHHHHHHCCCCEECCCCCCCC
PASGATFSMMPPDNATGNFNKVVQRIPVRISILPHPQIDLIKPGMSVEAKVDLRSQSS
CCCCCEEEECCCCCCCCCHHHHHHHCCEEEEECCCCCCEEECCCCCEEEEEECCCCCC
>Mature Secondary Structure
MDELSQTDDRQEPNYTRRKKVAWIVLILLFLGILLGILYYWFVYRFYESTENAYVKSDLT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCE
WIMPKVSGEVIELLAKENQYVEKDQPLVILDNRDLQARADQAKAMLALKQAGLSVQSENE
EEECHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
KSAQSTILEAQSARDAAQADVSRLSSDYRRYQQLLKEGVITRQRFEATQAEYLSAQAQLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAQAAIQAAQAQFSGVQAGRAQLYADLDNAKATVKLYDVDLGASEIQAPVAGKIGSLAVR
HHHHHHHHHHHHHHCCHHCHHHEEEECCCCEEEEEEEEECCCHHHHCCCCCCCHHHHHHH
LGSRVSPQTRLMAIIPKNSLYVEANFKETQIEKMRVGQTVSLVLDAYPSLTYRGKVESFA
HCCCCCCCCEEEEEEECCCEEEEECCCHHHHHHHHCCCHHHHHHHHCCCCEECCCCCCCC
PASGATFSMMPPDNATGNFNKVVQRIPVRISILPHPQIDLIKPGMSVEAKVDLRSQSS
CCCCCEEEECCCCCCCCCHHHHHHHCCEEEEECCCCCCEEECCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]