Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 50086618

GI number: 50086618

Start: 3594065

End: 3595453

Strand: Reverse

Name: trmE

Synonym: ACIAD3680

Alternate gene names: 50086618

Gene position: 3595453-3594065 (Counterclockwise)

Preceding gene: 50086619

Following gene: 50086617

Centisome position: 99.91

GC content: 46.29

Gene sequence:

>1389_bases
TTGATCTGTATTTTTCTGTTTTTAGGTTTCTATATGCAATATCAAACCACCATTGCTGCAATTGCAACGCCTCCAGGACG
TGGGGGTGTGGGGGTCATCCGGTTGTCAGGCCCAAAAGCATCTCTGATTGCTGAACATTTGACTGCAAAAACACTTCCAG
CTGCACGAATGGCAGGTTTTCGCCAGTTTTATGATGCTGAAGGGCTGGTGATGGATGAGGGGCTGGTGCTGTATTTTCCA
AATCCGAACTCATTTACAGGTGAAGATGTGGTTGAGTTGCAGGGACATGGCGGGCCTGTCATTCAGAATGCTTTGCTGGA
GCGATTGTTCGAGCTTGGTGCTAAAGCGGCCAAGGCGGGTGAGTTTTCCATGCGTGCCTTTGAAAATGGAAAGCTGGATC
TGGTACAAGCAGAAGCGATTGCTGATCTGATTGATGCGACATCACAAGCCGCTGCACGTTCGGCTGTACGTTCCTTGCAA
GGTGCTTTTTCGCTCAGAATTAATCAGGTTCTGGAAAAACTGATCCATTTACGCTTGCATGTAGAAGCGGCAATTGATTT
TCCTGAAGAAGAGATTGATTTTCTGGCAGATGGTAAAATTCTGGCTTTGCTGGATGACGTACGTGACTCGGTGCAACAGG
TTCAGACTTCTGCACGTCAGGGACAGTTATTGCGTGAAGGCTTACAAGTGGTGATTGCTGGTAAACCCAATGCAGGTAAG
TCTAGTTTACTCAATGCTCTGGCAGGCAATGAGCGCGCGATTGTGACCGATATTGCGGGAACCACGCGTGATGTTTTGCA
TGAAAGAATTAGCTTAAACGGTTTACCCATTACCTTGACCGATACCGCGGGTTTACGTGAAACAGGTGATATTGTCGAGA
AAGAAGGCATTCGACGTGCCATCAAGGAAATTGAACAAGCGGATTTATTACTACTGGTGTATGACTTAAGTGAGGGTGCA
GATCCACTGGCTTTGGCTCAGGAATATTTTGCTGAGCATCTGGAACCCCGACGCCTCATTTTGATTGGTAACAAATGTGA
TTTGATGACTGGTCATCCTGAGCTGGCTCAATATCAGAATTTTAGACATGTGACAGTATCTGCCAAAATGGACATGGGCG
TTCAGGCTTTGATCGATGCGATTACAGCGCATGCGGGCTTTCAGCCTGAAGAAGATACCTTTATTGCACGTACACGTCAT
CTCGATGCAATGAAACGCACTCAGCTGCATCTGCATGAGGCACGTGAGCAACTGGTTGTTTTCCATGCGGGTGAATTGGT
GGCTGAATCATTGCGACTTGCACAAAACGCTTTATCTGAAATTACGGGCGAATTTAGTGCAGATGACTTACTTGGCAAAA
TTTTTGGATCATTTTGTATCGGAAAATAG

Upstream 100 bases:

>100_bases
CAAAAGCTCGTGAAAATAAAGTGGAAACGACCTCATAATCGTTTGATACTTGAGAGCTGAATCAATCCGCTTAAGATGTT
ACGCTTAGGCGGATTTTTAT

Downstream 100 bases:

>100_bases
TGCTGAGTGATTGAGAAATATAAAAAGGCATTTGTTTGATTACAAATGCTTTTTTTATCTAAGTCACTGAATAAACTAAA
TGATAATAGTTCGCTATCAG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MICIFLFLGFYMQYQTTIAAIATPPGRGGVGVIRLSGPKASLIAEHLTAKTLPAARMAGFRQFYDAEGLVMDEGLVLYFP
NPNSFTGEDVVELQGHGGPVIQNALLERLFELGAKAAKAGEFSMRAFENGKLDLVQAEAIADLIDATSQAAARSAVRSLQ
GAFSLRINQVLEKLIHLRLHVEAAIDFPEEEIDFLADGKILALLDDVRDSVQQVQTSARQGQLLREGLQVVIAGKPNAGK
SSLLNALAGNERAIVTDIAGTTRDVLHERISLNGLPITLTDTAGLRETGDIVEKEGIRRAIKEIEQADLLLLVYDLSEGA
DPLALAQEYFAEHLEPRRLILIGNKCDLMTGHPELAQYQNFRHVTVSAKMDMGVQALIDAITAHAGFQPEEDTFIARTRH
LDAMKRTQLHLHEAREQLVVFHAGELVAESLRLAQNALSEITGEFSADDLLGKIFGSFCIGK

Sequences:

>Translated_462_residues
MICIFLFLGFYMQYQTTIAAIATPPGRGGVGVIRLSGPKASLIAEHLTAKTLPAARMAGFRQFYDAEGLVMDEGLVLYFP
NPNSFTGEDVVELQGHGGPVIQNALLERLFELGAKAAKAGEFSMRAFENGKLDLVQAEAIADLIDATSQAAARSAVRSLQ
GAFSLRINQVLEKLIHLRLHVEAAIDFPEEEIDFLADGKILALLDDVRDSVQQVQTSARQGQLLREGLQVVIAGKPNAGK
SSLLNALAGNERAIVTDIAGTTRDVLHERISLNGLPITLTDTAGLRETGDIVEKEGIRRAIKEIEQADLLLLVYDLSEGA
DPLALAQEYFAEHLEPRRLILIGNKCDLMTGHPELAQYQNFRHVTVSAKMDMGVQALIDAITAHAGFQPEEDTFIARTRH
LDAMKRTQLHLHEAREQLVVFHAGELVAESLRLAQNALSEITGEFSADDLLGKIFGSFCIGK
>Mature_462_residues
MICIFLFLGFYMQYQTTIAAIATPPGRGGVGVIRLSGPKASLIAEHLTAKTLPAARMAGFRQFYDAEGLVMDEGLVLYFP
NPNSFTGEDVVELQGHGGPVIQNALLERLFELGAKAAKAGEFSMRAFENGKLDLVQAEAIADLIDATSQAAARSAVRSLQ
GAFSLRINQVLEKLIHLRLHVEAAIDFPEEEIDFLADGKILALLDDVRDSVQQVQTSARQGQLLREGLQVVIAGKPNAGK
SSLLNALAGNERAIVTDIAGTTRDVLHERISLNGLPITLTDTAGLRETGDIVEKEGIRRAIKEIEQADLLLLVYDLSEGA
DPLALAQEYFAEHLEPRRLILIGNKCDLMTGHPELAQYQNFRHVTVSAKMDMGVQALIDAITAHAGFQPEEDTFIARTRH
LDAMKRTQLHLHEAREQLVVFHAGELVAESLRLAQNALSEITGEFSADDLLGKIFGSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=473, Percent_Identity=38.2663847780127, Blast_Score=261, Evalue=7e-70,
Organism=Homo sapiens, GI193082988, Length=501, Percent_Identity=36.3273453093812, Blast_Score=249, Evalue=5e-66,
Organism=Homo sapiens, GI193082993, Length=463, Percent_Identity=37.7969762419007, Blast_Score=245, Evalue=8e-65,
Organism=Escherichia coli, GI2367268, Length=455, Percent_Identity=55.6043956043956, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI87082120, Length=123, Percent_Identity=37.3983739837398, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=453, Percent_Identity=29.5805739514349, Blast_Score=195, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6323665, Length=503, Percent_Identity=32.6043737574553, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI45550813, Length=487, Percent_Identity=36.1396303901437, Blast_Score=244, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_ACIAD (Q6F6L1)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_048128.1
- HSSP:   P25522
- ProteinModelPortal:   Q6F6L1
- SMR:   Q6F6L1
- STRING:   Q6F6L1
- GeneID:   2880731
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3680
- NMPDR:   fig|62977.3.peg.3321
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q6F6L1
- ProtClustDB:   PRK05291
- BioCyc:   ASP62977:ACIAD3680-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 50323; Mature: 50323

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1

Important sites: BINDING 34-34 BINDING 92-92 BINDING 131-131 BINDING 462-462

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICIFLFLGFYMQYQTTIAAIATPPGRGGVGVIRLSGPKASLIAEHLTAKTLPAARMAGF
CCHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHH
RQFYDAEGLVMDEGLVLYFPNPNSFTGEDVVELQGHGGPVIQNALLERLFELGAKAAKAG
HHHHCCCCEEEECCEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCHHHHCCC
EFSMRAFENGKLDLVQAEAIADLIDATSQAAARSAVRSLQGAFSLRINQVLEKLIHLRLH
CHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VEAAIDFPEEEIDFLADGKILALLDDVRDSVQQVQTSARQGQLLREGLQVVIAGKPNAGK
EEHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCH
SSLLNALAGNERAIVTDIAGTTRDVLHERISLNGLPITLTDTAGLRETGDIVEKEGIRRA
HHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
IKEIEQADLLLLVYDLSEGADPLALAQEYFAEHLEPRRLILIGNKCDLMTGHPELAQYQN
HHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCC
FRHVTVSAKMDMGVQALIDAITAHAGFQPEEDTFIARTRHLDAMKRTQLHLHEAREQLVV
CEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
FHAGELVAESLRLAQNALSEITGEFSADDLLGKIFGSFCIGK
EECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MICIFLFLGFYMQYQTTIAAIATPPGRGGVGVIRLSGPKASLIAEHLTAKTLPAARMAGF
CCHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHH
RQFYDAEGLVMDEGLVLYFPNPNSFTGEDVVELQGHGGPVIQNALLERLFELGAKAAKAG
HHHHCCCCEEEECCEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCHHHHCCC
EFSMRAFENGKLDLVQAEAIADLIDATSQAAARSAVRSLQGAFSLRINQVLEKLIHLRLH
CHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VEAAIDFPEEEIDFLADGKILALLDDVRDSVQQVQTSARQGQLLREGLQVVIAGKPNAGK
EEHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCH
SSLLNALAGNERAIVTDIAGTTRDVLHERISLNGLPITLTDTAGLRETGDIVEKEGIRRA
HHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
IKEIEQADLLLLVYDLSEGADPLALAQEYFAEHLEPRRLILIGNKCDLMTGHPELAQYQN
HHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCC
FRHVTVSAKMDMGVQALIDAITAHAGFQPEEDTFIARTRHLDAMKRTQLHLHEAREQLVV
CEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
FHAGELVAESLRLAQNALSEITGEFSADDLLGKIFGSFCIGK
EECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA