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The map label for this gene is emrB [H]

Identifier: 50086616

GI number: 50086616

Start: 3590952

End: 3592502

Strand: Reverse

Name: emrB [H]

Synonym: ACIAD3678

Alternate gene names: 50086616

Gene position: 3592502-3590952 (Counterclockwise)

Preceding gene: 50086617

Following gene: 50086615

Centisome position: 99.83

GC content: 43.33

Gene sequence:

>1551_bases
ATGACTATGTCTTCCGCTTCCATAGAATGGCAATTTTCTCCGCGTGTTGCCTGGCTGATCTTTATTGCCATGATTTTTGG
GAATTTCATGGCAATTCTGGATATCCAGATTGTTGCCAGTTCCTTAAATGAAATTCAGGCAGGCATGAGCGCCAGTCGTT
ATGAAGTGACTTGGGTGCAAACGGTATATCTAATTGCAGAAATTATTGCGATTCCGATGTCGAGTGTGCTTTCCAGAATT
TTGTCTACCCGAATATATTATGCACTCTGTGCCACAGGATTTACCATTAGCTCCCTGCTCTGTGCACTGGCATGGAATCT
GGAAAGTTTACTGGTGTTTCGTGCTTTTCAGGGTTTTATGGGCGGAGGTATGATACCGACCTCAATGACCGCCCTATTCT
TGTTGTTTCCAGAAGCCAAGCGTGCTGTTCCATTGGTATTGTTTGGCATGATTAGTACTTTGGGGCCAGCCATTGGTCCA
ACTGTGGGTGGCTGGCTAACGACCAGTTTTTCATGGCACTGGATGTTTCTGATCAATATTATCCCAGGCATCATTATTTC
CGTCGTCATTTATGCCAGTCAGGCGATTGATCAGGCCAATTACAAACTACTTAAGGAACTGGATTGGCTAAGCCTAATTG
GGATGGCAATGTTTCTGGGCGGACTGGAATACTTTCTAGATGAAGGGGGACGCTATGACTGGTTCGCCGATTTTGGTGTA
CGTATCGCATTTCTGGTTAGTCTGTTGGGTGCCATGATTTTCTTCTATCGCAGTTATGTTCAACCCAAACCCTTGCTTGA
TTTGAGTGTATTTAAAAACAAGAACTTTACTTTAAGTTGTATGATTACATTTGTGATTGGTATGGCCTTATACGGTTTGG
GCTATATGATTCCAGTGTTCTTAGGTCAGGTTCGTGAGATGAACAGTAGTCAGATTGGGCATATCATGATGGTGACAGGC
ATTGTGATGTTTTTCTTTGCGCCTGTACTGGCCTGGCTTATTCCTAATTTTGATGCACGCAAAACGGTTTTTATTGGCAT
GTTAATGGCTGCCTTTGGTGTATGGCTGAATGCGCACCTAACCATTCAGAGTGATTACGATTTTATGTTTTGGCCACAAG
TATATCGTGGCATTGGTTTGATGATTTGTCTGATTGTGATTTCACATTTAGCGATGAGCACCTTACCAGAACACAAAATT
GCCGATGCCAGTGGTATCTATAACTTGATGCGTAACATTGGTGGTGCAGTTGGTCTGGCGCTGATTAACTCACGTATTGA
CTGGCTTACGGCATGGCATGCCACTGAACTGAATAGCCAGCTTACGCCTGCAAACTGGATTTTTGTCGAACGACTGGCGC
AATTAAAAGCACAGTATGCAGAGCTGGGCGATCTTTCGGAACAGCTGGCCATGCAAGTGATTTACAGACAAGTGCATTAT
CAGGCGCTTGCTGCAAGTTTTAATGATCTATTGAAATTACTGGCAATCATCATGCTAGTGGTTGCTTTTCTTACATTTTT
TATGGATAGGGGGAAAAAGGTATCCTTTTAA

Upstream 100 bases:

>100_bases
TCAAATTGATTTGATTAAACCAGGGATGTCGGTCGAGGCAAAAGTTGATTTAAGAAGTCAATCAAGCTGACGACATACAG
CATAGGAGGGATGAACTGAG

Downstream 100 bases:

>100_bases
ATACTGTAGAGCAGTGAAAGCGGACTTAATCTTAAGACATTGGATGTCTTTAAAACACGATGTTTTCAATCGACTTACTC
TCTAAAAGATTGAATCCATC

Product: MFS superfamily multidrug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTMSSASIEWQFSPRVAWLIFIAMIFGNFMAILDIQIVASSLNEIQAGMSASRYEVTWVQTVYLIAEIIAIPMSSVLSRI
LSTRIYYALCATGFTISSLLCALAWNLESLLVFRAFQGFMGGGMIPTSMTALFLLFPEAKRAVPLVLFGMISTLGPAIGP
TVGGWLTTSFSWHWMFLINIIPGIIISVVIYASQAIDQANYKLLKELDWLSLIGMAMFLGGLEYFLDEGGRYDWFADFGV
RIAFLVSLLGAMIFFYRSYVQPKPLLDLSVFKNKNFTLSCMITFVIGMALYGLGYMIPVFLGQVREMNSSQIGHIMMVTG
IVMFFFAPVLAWLIPNFDARKTVFIGMLMAAFGVWLNAHLTIQSDYDFMFWPQVYRGIGLMICLIVISHLAMSTLPEHKI
ADASGIYNLMRNIGGAVGLALINSRIDWLTAWHATELNSQLTPANWIFVERLAQLKAQYAELGDLSEQLAMQVIYRQVHY
QALAASFNDLLKLLAIIMLVVAFLTFFMDRGKKVSF

Sequences:

>Translated_516_residues
MTMSSASIEWQFSPRVAWLIFIAMIFGNFMAILDIQIVASSLNEIQAGMSASRYEVTWVQTVYLIAEIIAIPMSSVLSRI
LSTRIYYALCATGFTISSLLCALAWNLESLLVFRAFQGFMGGGMIPTSMTALFLLFPEAKRAVPLVLFGMISTLGPAIGP
TVGGWLTTSFSWHWMFLINIIPGIIISVVIYASQAIDQANYKLLKELDWLSLIGMAMFLGGLEYFLDEGGRYDWFADFGV
RIAFLVSLLGAMIFFYRSYVQPKPLLDLSVFKNKNFTLSCMITFVIGMALYGLGYMIPVFLGQVREMNSSQIGHIMMVTG
IVMFFFAPVLAWLIPNFDARKTVFIGMLMAAFGVWLNAHLTIQSDYDFMFWPQVYRGIGLMICLIVISHLAMSTLPEHKI
ADASGIYNLMRNIGGAVGLALINSRIDWLTAWHATELNSQLTPANWIFVERLAQLKAQYAELGDLSEQLAMQVIYRQVHY
QALAASFNDLLKLLAIIMLVVAFLTFFMDRGKKVSF
>Mature_515_residues
TMSSASIEWQFSPRVAWLIFIAMIFGNFMAILDIQIVASSLNEIQAGMSASRYEVTWVQTVYLIAEIIAIPMSSVLSRIL
STRIYYALCATGFTISSLLCALAWNLESLLVFRAFQGFMGGGMIPTSMTALFLLFPEAKRAVPLVLFGMISTLGPAIGPT
VGGWLTTSFSWHWMFLINIIPGIIISVVIYASQAIDQANYKLLKELDWLSLIGMAMFLGGLEYFLDEGGRYDWFADFGVR
IAFLVSLLGAMIFFYRSYVQPKPLLDLSVFKNKNFTLSCMITFVIGMALYGLGYMIPVFLGQVREMNSSQIGHIMMVTGI
VMFFFAPVLAWLIPNFDARKTVFIGMLMAAFGVWLNAHLTIQSDYDFMFWPQVYRGIGLMICLIVISHLAMSTLPEHKIA
DASGIYNLMRNIGGAVGLALINSRIDWLTAWHATELNSQLTPANWIFVERLAQLKAQYAELGDLSEQLAMQVIYRQVHYQ
ALAASFNDLLKLLAIIMLVVAFLTFFMDRGKKVSF

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=491, Percent_Identity=26.4765784114053, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1788710, Length=493, Percent_Identity=28.3975659229209, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1790195, Length=283, Percent_Identity=28.6219081272085, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87081983, Length=407, Percent_Identity=25.5528255528256, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788392, Length=302, Percent_Identity=26.4900662251656, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57815; Mature: 57683

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMSSASIEWQFSPRVAWLIFIAMIFGNFMAILDIQIVASSLNEIQAGMSASRYEVTWVQ
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHH
TVYLIAEIIAIPMSSVLSRILSTRIYYALCATGFTISSLLCALAWNLESLLVFRAFQGFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGGMIPTSMTALFLLFPEAKRAVPLVLFGMISTLGPAIGPTVGGWLTTSFSWHWMFLINI
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHH
IPGIIISVVIYASQAIDQANYKLLKELDWLSLIGMAMFLGGLEYFLDEGGRYDWFADFGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
RIAFLVSLLGAMIFFYRSYVQPKPLLDLSVFKNKNFTLSCMITFVIGMALYGLGYMIPVF
HHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LGQVREMNSSQIGHIMMVTGIVMFFFAPVLAWLIPNFDARKTVFIGMLMAAFGVWLNAHL
HHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEE
TIQSDYDFMFWPQVYRGIGLMICLIVISHLAMSTLPEHKIADASGIYNLMRNIGGAVGLA
EEECCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHH
LINSRIDWLTAWHATELNSQLTPANWIFVERLAQLKAQYAELGDLSEQLAMQVIYRQVHY
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QALAASFNDLLKLLAIIMLVVAFLTFFMDRGKKVSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TMSSASIEWQFSPRVAWLIFIAMIFGNFMAILDIQIVASSLNEIQAGMSASRYEVTWVQ
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHH
TVYLIAEIIAIPMSSVLSRILSTRIYYALCATGFTISSLLCALAWNLESLLVFRAFQGFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGGMIPTSMTALFLLFPEAKRAVPLVLFGMISTLGPAIGPTVGGWLTTSFSWHWMFLINI
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHH
IPGIIISVVIYASQAIDQANYKLLKELDWLSLIGMAMFLGGLEYFLDEGGRYDWFADFGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
RIAFLVSLLGAMIFFYRSYVQPKPLLDLSVFKNKNFTLSCMITFVIGMALYGLGYMIPVF
HHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LGQVREMNSSQIGHIMMVTGIVMFFFAPVLAWLIPNFDARKTVFIGMLMAAFGVWLNAHL
HHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEE
TIQSDYDFMFWPQVYRGIGLMICLIVISHLAMSTLPEHKIADASGIYNLMRNIGGAVGLA
EEECCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHH
LINSRIDWLTAWHATELNSQLTPANWIFVERLAQLKAQYAELGDLSEQLAMQVIYRQVHY
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QALAASFNDLLKLLAIIMLVVAFLTFFMDRGKKVSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]