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The map label for this gene is recJ [H]

Identifier: 50086458

GI number: 50086458

Start: 3424072

End: 3425778

Strand: Reverse

Name: recJ [H]

Synonym: ACIAD3500

Alternate gene names: 50086458

Gene position: 3425778-3424072 (Counterclockwise)

Preceding gene: 50086459

Following gene: 50086456

Centisome position: 95.2

GC content: 44.05

Gene sequence:

>1707_bases
ATGCGCACAATTCAAATCAAACAGCGACCGTTACTCACACATCCTGATCAATTTCAGGATGTGCCCGAATTTATTGCGCA
TATTTTAGCCCGTCGAGGTGTCGACTCTATTCAGCAGTTAGATTTAAAGCTGAAATATTTGCTCAAGCCAACATTAAAGG
GCTTGGATACTGCAATTGCGCTGATTGATCAAGCCATCGACCAGCAACAGCAGATTATTATTGTGGGTGACTATGATGCA
GATGGTGCAACCAGTACGGCTTTGATGTGTCTAGCTTTAAAAGAAATGGGCGCAAGAGTTGATTACATTGTGCCTGATCG
CTTTAAGTATGGTTATGGCTTAACCCCTGCAATTGCTGATCTGGCGCATCAACAATGTCAACCAGAGCTGTTGATCACTG
TCGATAATGGTATTTCAAGTCATGCTGGTGTAGAGCAAGCACAGCAACTGGGCATGAAAGTGATTATTACCGATCATCAC
TTAACGACCAAATCTGCACCAGATGCTGAAGCCGTGGTTAATCCGAATCAACTTAATTGTCCGTTTCCAAGTAAAGCATT
GGCAGGGGTCGGGGTTGCATTTTATCTGTTGGCACATTTATCGAGCTTAAGGCAGGCACATCACAAACCAAGTTGCAAAA
TCACGCAATATCTGGATTTGGTCGCATTGGGTACATATGCCGATGTCGCGAGCTTAGATTATAACAATCGGATTTTGGTC
GATGCAGGTTTAAAACGGATTCAGCAACAGCAATGCCGTGCTGGCATACTGGCATTACTGGATATCGCTAAACGTGATCC
TTCGACGTTAAAAGCACAAGATTTAGGATTTGTACTTGGACCACGCATTAATGCAGCAGGTCGGATGGACAGCATGAATA
TCGGCATTGAATGCCTGCTTGCAAGTGATCTGGAAACTGCCTATCCGATTGCAGAGCAACTGAATCGACTTAATCTTGAG
CGTCGTCAGGTCGAAACGGAGATGAAACAGCAGGCACTTGGCTATCTTGAAGATATTCAGTTACAGCATGAAAATATTCC
GCCTGCCTTAGTCATGTTTGCAGAAAACTGGCATCAAGGTGTGATTGGAATTGTGGCGGGACGTCTTAAAGAGCAGTTTC
ATCGCCCCAGCATTGTATTTGCACCTGATACGGACGGGGAGCATATTAAAGGATCTGCCCGTTCTATCGAAGGTATTCAC
ATCCGCGATACGATTGAACAGGTTGCAGAACAACATCCTCATTTAGTCAGTCATTTTGGGGGGCATGCGGCGGCGGCGGG
CTTAACCCTGAAAAAAAGTCACTTTGAAGAATTTAAAAACGTATTCACAGCGTGTATTGCGCAAATGGATGAAACTTTGC
TGCAAGCGGTGCTCTGGACCGATGGCTCTTTGCCTGCACAGGCTATTGATCTGGAAACTGTCGACTGGATCGAGCGTCTA
GGGCCTTGGGGACAAAAGTTCCCACTTCCACAGTTTGAAGGACGTTTTGAAGTGCTGGATTATCGCTGGCTGAAAGATAT
ACATCTCAAGTTAAAACTTGGCTTGGGAGAATATGGCTCTGTAGATGCGATTGCATTTAATGCAGCACAAAAGTTTGATT
TTGATCCAATGAAGAAACATGTCAATTTGGTATATGAGCTAGATCGTAATGAATTTAATGGCAGTGTAAATTTACAACTT
CGCATCTTACATTTAGATCAGACATAA

Upstream 100 bases:

>100_bases
TTATTACGAGTTCTGGCAGGGGCGTCCGAATCGCTTACATGACCGTTTAAGTTATCAAAACACAGATGGCACATGGGTTG
TGCAACGACTGATGCCATAA

Downstream 100 bases:

>100_bases
AAAAAATCGCTCATTTGAGCGATTTTTTTTATGTTCTTAAGCAGTGCTTAGAAACGGTATGATGCACCCACTGAGTAAGT
ATTGTAGTCATCACCAAAAC

Product: ssDNA exonuclease, 5'--> 3' specific, Mg dependent

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MRTIQIKQRPLLTHPDQFQDVPEFIAHILARRGVDSIQQLDLKLKYLLKPTLKGLDTAIALIDQAIDQQQQIIIVGDYDA
DGATSTALMCLALKEMGARVDYIVPDRFKYGYGLTPAIADLAHQQCQPELLITVDNGISSHAGVEQAQQLGMKVIITDHH
LTTKSAPDAEAVVNPNQLNCPFPSKALAGVGVAFYLLAHLSSLRQAHHKPSCKITQYLDLVALGTYADVASLDYNNRILV
DAGLKRIQQQQCRAGILALLDIAKRDPSTLKAQDLGFVLGPRINAAGRMDSMNIGIECLLASDLETAYPIAEQLNRLNLE
RRQVETEMKQQALGYLEDIQLQHENIPPALVMFAENWHQGVIGIVAGRLKEQFHRPSIVFAPDTDGEHIKGSARSIEGIH
IRDTIEQVAEQHPHLVSHFGGHAAAAGLTLKKSHFEEFKNVFTACIAQMDETLLQAVLWTDGSLPAQAIDLETVDWIERL
GPWGQKFPLPQFEGRFEVLDYRWLKDIHLKLKLGLGEYGSVDAIAFNAAQKFDFDPMKKHVNLVYELDRNEFNGSVNLQL
RILHLDQT

Sequences:

>Translated_568_residues
MRTIQIKQRPLLTHPDQFQDVPEFIAHILARRGVDSIQQLDLKLKYLLKPTLKGLDTAIALIDQAIDQQQQIIIVGDYDA
DGATSTALMCLALKEMGARVDYIVPDRFKYGYGLTPAIADLAHQQCQPELLITVDNGISSHAGVEQAQQLGMKVIITDHH
LTTKSAPDAEAVVNPNQLNCPFPSKALAGVGVAFYLLAHLSSLRQAHHKPSCKITQYLDLVALGTYADVASLDYNNRILV
DAGLKRIQQQQCRAGILALLDIAKRDPSTLKAQDLGFVLGPRINAAGRMDSMNIGIECLLASDLETAYPIAEQLNRLNLE
RRQVETEMKQQALGYLEDIQLQHENIPPALVMFAENWHQGVIGIVAGRLKEQFHRPSIVFAPDTDGEHIKGSARSIEGIH
IRDTIEQVAEQHPHLVSHFGGHAAAAGLTLKKSHFEEFKNVFTACIAQMDETLLQAVLWTDGSLPAQAIDLETVDWIERL
GPWGQKFPLPQFEGRFEVLDYRWLKDIHLKLKLGLGEYGSVDAIAFNAAQKFDFDPMKKHVNLVYELDRNEFNGSVNLQL
RILHLDQT
>Mature_568_residues
MRTIQIKQRPLLTHPDQFQDVPEFIAHILARRGVDSIQQLDLKLKYLLKPTLKGLDTAIALIDQAIDQQQQIIIVGDYDA
DGATSTALMCLALKEMGARVDYIVPDRFKYGYGLTPAIADLAHQQCQPELLITVDNGISSHAGVEQAQQLGMKVIITDHH
LTTKSAPDAEAVVNPNQLNCPFPSKALAGVGVAFYLLAHLSSLRQAHHKPSCKITQYLDLVALGTYADVASLDYNNRILV
DAGLKRIQQQQCRAGILALLDIAKRDPSTLKAQDLGFVLGPRINAAGRMDSMNIGIECLLASDLETAYPIAEQLNRLNLE
RRQVETEMKQQALGYLEDIQLQHENIPPALVMFAENWHQGVIGIVAGRLKEQFHRPSIVFAPDTDGEHIKGSARSIEGIH
IRDTIEQVAEQHPHLVSHFGGHAAAAGLTLKKSHFEEFKNVFTACIAQMDETLLQAVLWTDGSLPAQAIDLETVDWIERL
GPWGQKFPLPQFEGRFEVLDYRWLKDIHLKLKLGLGEYGSVDAIAFNAAQKFDFDPMKKHVNLVYELDRNEFNGSVNLQL
RILHLDQT

Specific function: Single-stranded-DNA-specific exonuclease. Required for many types of recombinational events, although the stringency of the requirement for recJ appears to vary with the type of recombinational event monitored and the other recombination gene products whi

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recJ family [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=569, Percent_Identity=44.1124780316344, Blast_Score=476, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 63268; Mature: 63268

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIQIKQRPLLTHPDQFQDVPEFIAHILARRGVDSIQQLDLKLKYLLKPTLKGLDTAIA
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIDQAIDQQQQIIIVGDYDADGATSTALMCLALKEMGARVDYIVPDRFKYGYGLTPAIAD
HHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHH
LAHQQCQPELLITVDNGISSHAGVEQAQQLGMKVIITDHHLTTKSAPDAEAVVNPNQLNC
HHHHCCCCCEEEEECCCCCCCCCHHHHHHCCEEEEEECCCEECCCCCCCCCEECCCCCCC
PFPSKALAGVGVAFYLLAHLSSLRQAHHKPSCKITQYLDLVALGTYADVASLDYNNRILV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHCCCCCEEEE
DAGLKRIQQQQCRAGILALLDIAKRDPSTLKAQDLGFVLGPRINAAGRMDSMNIGIECLL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEECCCCCCCCCCCCCCCCEEEEE
ASDLETAYPIAEQLNRLNLERRQVETEMKQQALGYLEDIQLQHENIPPALVMFAENWHQG
ECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
VIGIVAGRLKEQFHRPSIVFAPDTDGEHIKGSARSIEGIHIRDTIEQVAEQHPHLVSHFG
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCHHHHHCC
GHAAAAGLTLKKSHFEEFKNVFTACIAQMDETLLQAVLWTDGSLPAQAIDLETVDWIERL
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHH
GPWGQKFPLPQFEGRFEVLDYRWLKDIHLKLKLGLGEYGSVDAIAFNAAQKFDFDPMKKH
CCCCCCCCCCCCCCCEEEEHHHHHHEEEEEEEECCCCCCCCCEEEECCHHCCCCCHHHHH
VNLVYELDRNEFNGSVNLQLRILHLDQT
CEEEEEECCCCCCCEEEEEEEEEEECCC
>Mature Secondary Structure
MRTIQIKQRPLLTHPDQFQDVPEFIAHILARRGVDSIQQLDLKLKYLLKPTLKGLDTAIA
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIDQAIDQQQQIIIVGDYDADGATSTALMCLALKEMGARVDYIVPDRFKYGYGLTPAIAD
HHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHH
LAHQQCQPELLITVDNGISSHAGVEQAQQLGMKVIITDHHLTTKSAPDAEAVVNPNQLNC
HHHHCCCCCEEEEECCCCCCCCCHHHHHHCCEEEEEECCCEECCCCCCCCCEECCCCCCC
PFPSKALAGVGVAFYLLAHLSSLRQAHHKPSCKITQYLDLVALGTYADVASLDYNNRILV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHCCCCCEEEE
DAGLKRIQQQQCRAGILALLDIAKRDPSTLKAQDLGFVLGPRINAAGRMDSMNIGIECLL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEECCCCCCCCCCCCCCCCEEEEE
ASDLETAYPIAEQLNRLNLERRQVETEMKQQALGYLEDIQLQHENIPPALVMFAENWHQG
ECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
VIGIVAGRLKEQFHRPSIVFAPDTDGEHIKGSARSIEGIHIRDTIEQVAEQHPHLVSHFG
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCHHHHHCC
GHAAAAGLTLKKSHFEEFKNVFTACIAQMDETLLQAVLWTDGSLPAQAIDLETVDWIERL
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHH
GPWGQKFPLPQFEGRFEVLDYRWLKDIHLKLKLGLGEYGSVDAIAFNAAQKFDFDPMKKH
CCCCCCCCCCCCCCCEEEEHHHHHHEEEEEEEECCCCCCCCCEEEECCHHCCCCCHHHHH
VNLVYELDRNEFNGSVNLQLRILHLDQT
CEEEEEECCCCCCCEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8168497 [H]