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The map label for this gene is prfB [H]

Identifier: 50086456

GI number: 50086456

Start: 3421705

End: 3422718

Strand: Reverse

Name: prfB [H]

Synonym: ACIAD3498

Alternate gene names: 50086456

Gene position: 3422718-3421705 (Counterclockwise)

Preceding gene: 50086458

Following gene: 50086455

Centisome position: 95.11

GC content: 46.35

Gene sequence:

>1014_bases
CTGAAAAAAGAGCGTTTAGAAGAAGTTTTACGTGAACTTGAAGATCCTGCGATCTGGAATGATCAGAGTCGTGCACAAGC
CATGGCCAAAGAAAAAGGCGAGCTTGAAAACGTACTGAATGTGCTAGATAACCTTGCTGAACAGTTAGAAGATGCCAAGG
CGATGCTGGATCTGGCGGTCGAAGCCGATGATGAAAGTTTACTGGTTGATGTGGAATCAGAACTTGCAACGGCTGAGGAC
GAACTCGCCAAGCTTGAATTCCGTCGTATGTTTAGTAATCCGATGGATCCAAATCCGTGTTATGTGGAAATTCAGGCGGG
TTCAGGTGGAACAGAGGCTCAGGATTGGGCTTCTATGTTGCTGCGTATGTACATGCGCTGGATTGAGCGTCACGGCTTTA
AAGCTGAATTGATGGAAGAGTCTGATGGTGATGTTGCAGGCATTAAATCAGCAACGATTCGTGTAGAAGGTGAGTACGCA
TATGGCTGGTTACGTACCGAGTCGGGTGTACATCGTCTCGTTCGAAAATCTCCTTTTGACAGTGGTAACCGTCGTCATAC
CTCGTTTAGTGCGGTGTTTGTTTCGCCAGAAATTGATGACAACATTGAAATTGAGATCAATCCTGCGGATGTGCGTACCG
ACACCTATCGTGCTTCTGGAGCCGGTGGTCAGCATATTAACAAAACCGATTCGGCTGTGCGTTTGACGCATGCGCCAACA
GGTATTGTTGTTGCATGTCAAAACCAGCGTTCACAACATGCCAACCGTGATCATGCATGGAAACAGTTACGTGCCAAGTT
GTATGAACTGGAAATGAGTAAGCGTAATGAAGCTGCTCAGGCACTTGAAGATTCCAAATCGGACATCGGTTGGGGTAGCC
AGATCCGCTCGTACGTACTGGATGATTCTCGTATTAAAGATTTGCGTACAGGTGTCGAAAACTCAAATACAGGTGCTGTT
CTGGATGGTGATTTGGATAAATTTATCGAAGCCAGCTTGAAACAGGGTCTCTAA

Upstream 100 bases:

>100_bases
TAATTTGGGAATTTTGGCGTGGAAATTAATCCGTATCTAAACCAATTAAAAGATTTAACCGAACGTAGTCAAACACTACG
GGGGTATCTTTGACTACGAT

Downstream 100 bases:

>100_bases
TTGTGAACGTTAGCAAAGCATATTTACGTCTGCTTACACTATATCTATAAAAATCGATATTAATATTGTAAAAAGAGAAT
ATTAATATCGAAAAAAAACG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKKERLEEVLRELEDPAIWNDQSRAQAMAKEKGELENVLNVLDNLAEQLEDAKAMLDLAVEADDESLLVDVESELATAED
ELAKLEFRRMFSNPMDPNPCYVEIQAGSGGTEAQDWASMLLRMYMRWIERHGFKAELMEESDGDVAGIKSATIRVEGEYA
YGWLRTESGVHRLVRKSPFDSGNRRHTSFSAVFVSPEIDDNIEIEINPADVRTDTYRASGAGGQHINKTDSAVRLTHAPT
GIVVACQNQRSQHANRDHAWKQLRAKLYELEMSKRNEAAQALEDSKSDIGWGSQIRSYVLDDSRIKDLRTGVENSNTGAV
LDGDLDKFIEASLKQGL

Sequences:

>Translated_337_residues
MKKERLEEVLRELEDPAIWNDQSRAQAMAKEKGELENVLNVLDNLAEQLEDAKAMLDLAVEADDESLLVDVESELATAED
ELAKLEFRRMFSNPMDPNPCYVEIQAGSGGTEAQDWASMLLRMYMRWIERHGFKAELMEESDGDVAGIKSATIRVEGEYA
YGWLRTESGVHRLVRKSPFDSGNRRHTSFSAVFVSPEIDDNIEIEINPADVRTDTYRASGAGGQHINKTDSAVRLTHAPT
GIVVACQNQRSQHANRDHAWKQLRAKLYELEMSKRNEAAQALEDSKSDIGWGSQIRSYVLDDSRIKDLRTGVENSNTGAV
LDGDLDKFIEASLKQGL
>Mature_337_residues
MKKERLEEVLRELEDPAIWNDQSRAQAMAKEKGELENVLNVLDNLAEQLEDAKAMLDLAVEADDESLLVDVESELATAED
ELAKLEFRRMFSNPMDPNPCYVEIQAGSGGTEAQDWASMLLRMYMRWIERHGFKAELMEESDGDVAGIKSATIRVEGEYA
YGWLRTESGVHRLVRKSPFDSGNRRHTSFSAVFVSPEIDDNIEIEINPADVRTDTYRASGAGGQHINKTDSAVRLTHAPT
GIVVACQNQRSQHANRDHAWKQLRAKLYELEMSKRNEAAQALEDSKSDIGWGSQIRSYVLDDSRIKDLRTGVENSNTGAV
LDGDLDKFIEASLKQGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=314, Percent_Identity=33.7579617834395, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI34577120, Length=323, Percent_Identity=32.1981424148607, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI166795305, Length=213, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI2367172, Length=336, Percent_Identity=69.3452380952381, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1787462, Length=342, Percent_Identity=36.8421052631579, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=281, Percent_Identity=34.8754448398576, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=34.4936708860759, Blast_Score=158, Evalue=1e-39,
Organism=Drosophila melanogaster, GI19921226, Length=231, Percent_Identity=37.6623376623377, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37802; Mature: 37802

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKERLEEVLRELEDPAIWNDQSRAQAMAKEKGELENVLNVLDNLAEQLEDAKAMLDLAV
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
EADDESLLVDVESELATAEDELAKLEFRRMFSNPMDPNPCYVEIQAGSGGTEAQDWASML
ECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHH
LRMYMRWIERHGFKAELMEESDGDVAGIKSATIRVEGEYAYGWLRTESGVHRLVRKSPFD
HHHHHHHHHHCCCCEEHHCCCCCCEECCCEEEEEEECCEEEEEEECHHHHHHHHHCCCCC
SGNRRHTSFSAVFVSPEIDDNIEIEINPADVRTDTYRASGAGGQHINKTDSAVRLTHAPT
CCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCEECCCCCCCCCCCCCCEEEEEECCC
GIVVACQNQRSQHANRDHAWKQLRAKLYELEMSKRNEAAQALEDSKSDIGWGSQIRSYVL
CEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
DDSRIKDLRTGVENSNTGAVLDGDLDKFIEASLKQGL
CCHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKERLEEVLRELEDPAIWNDQSRAQAMAKEKGELENVLNVLDNLAEQLEDAKAMLDLAV
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
EADDESLLVDVESELATAEDELAKLEFRRMFSNPMDPNPCYVEIQAGSGGTEAQDWASML
ECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHH
LRMYMRWIERHGFKAELMEESDGDVAGIKSATIRVEGEYAYGWLRTESGVHRLVRKSPFD
HHHHHHHHHHCCCCEEHHCCCCCCEECCCEEEEEEECCEEEEEEECHHHHHHHHHCCCCC
SGNRRHTSFSAVFVSPEIDDNIEIEINPADVRTDTYRASGAGGQHINKTDSAVRLTHAPT
CCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCEECCCCCCCCCCCCCCEEEEEECCC
GIVVACQNQRSQHANRDHAWKQLRAKLYELEMSKRNEAAQALEDSKSDIGWGSQIRSYVL
CEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
DDSRIKDLRTGVENSNTGAVLDGDLDKFIEASLKQGL
CCHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA