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The map label for this gene is cca

Identifier: 50085387

GI number: 50085387

Start: 2259572

End: 2260813

Strand: Direct

Name: cca

Synonym: ACIAD2288

Alternate gene names: 50085387

Gene position: 2259572-2260813 (Clockwise)

Preceding gene: 50085386

Following gene: 50085388

Centisome position: 62.79

GC content: 40.42

Gene sequence:

>1242_bases
ATGCAAGTGTATTTAGTAGGCGGTGCCGTTCGTGATCACTTATTAGGCCACCCCTATCACGAAAAAGATTATGTTGTGGT
GGGCGCGTCACCCCAACAATTATTAGACCAAGGATTTTCACCAGTTGGAAAGGATTTTCCTGTATTTCTTCATCCCAAAA
CCAAAGAAGAATATGCATTAGCACGTACAGAAAGAAAATCAGGCATTGGTTATCATGGTTTTAACTTTTTCACCGATCCT
GATGTAAGCCTTGAAGATGATTTAATTCGTAGAGACCTTACTATCAATGCGATGGCCATGGATCAAGACGGTAATGTTTA
TGATCCTTACGGTGGACAAAAAGACCTAGAGCAACGTATTTTACGTCACGTATCCGATGCATTTATTGAGGATCCACTGC
GAGTACTTAGAGTGGCTCGTTTTGCAGCGCGATATGCCCGTTATGGTTTCAAAATTGCAGATGAAACACTTTTGCTGATG
CAATCCATTACTCAGACTGGAGAACTAGAAAGCCTGACTGCTGAACGGGTATGGAAAGAAACATCACGCGCCCTGATGGA
AGATCAAGCAGATATTTATTTTGATGTTTTGCGCCAATGTCATGCATTAAAAGTCTTATTTCCTGAAATTGATGCCTTAT
TCGGTGTCCCTCAACGCCCTGAATATCATCCTGAAATCGACTGTGGCATCCATACATTGATGTCTTTAAGACAAGCTTGT
AAATCGAACTATTCATTAGATGTTCGTTATGCGGTGCTTGTACATGATTTAGGTAAAGCACTAACCCCTAAAGATATACT
TCCAAGACATATCATGCATGAGGAAAGAGGCCTTATCCCTGTTGAAGAACTTTCAAATCGCTTGAAAATTCCAACTCAAA
TGAAGCAATTGGCACTGGCTGTGTGTAAGGAACATCTCAAGTGTCATCAAGCGATGTCTCTTAAGCCAGGTACATTGTGG
CGTTTATTACAACGTCTAGATGTATTACGTCGTCCTGAACGCGTAGAGGCTTTTATAAACGCGTGTGAATGTGATGCACG
AGGTCGTTTAGGACTAGAAAATCGTGATTATCCACAAGCTGCATTCATGTTTAAAGCTATGCAAGTTGTTAGATCCATCA
AAGCACAAGATTTACCAGCTTACATACAAGGTTCCGAAATTGGTGAAAAACTGATTCAATATCGTATCGATGCTTTAGCT
GAACTCAAAAAACAATACGATGATTCGTTAGCAACAGAATAA

Upstream 100 bases:

>100_bases
AAGCGAAGGTTAATCCTTTGCTTTATTGAAAAGCACAACTTTGTGACTTGTGAAAACCTCTGTTCTGTTGCATGATTGCT
TTGAGAACAGAGGTTTTTTC

Downstream 100 bases:

>100_bases
GAAAAGCGCCATGAAGTGATAAGCAATTATTTCATGGTGCTTTTTATTATATTAAAATCGAATTAATTAAAAATTTTAAT
ATTTATCAATTAATTAAATT

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MQVYLVGGAVRDHLLGHPYHEKDYVVVGASPQQLLDQGFSPVGKDFPVFLHPKTKEEYALARTERKSGIGYHGFNFFTDP
DVSLEDDLIRRDLTINAMAMDQDGNVYDPYGGQKDLEQRILRHVSDAFIEDPLRVLRVARFAARYARYGFKIADETLLLM
QSITQTGELESLTAERVWKETSRALMEDQADIYFDVLRQCHALKVLFPEIDALFGVPQRPEYHPEIDCGIHTLMSLRQAC
KSNYSLDVRYAVLVHDLGKALTPKDILPRHIMHEERGLIPVEELSNRLKIPTQMKQLALAVCKEHLKCHQAMSLKPGTLW
RLLQRLDVLRRPERVEAFINACECDARGRLGLENRDYPQAAFMFKAMQVVRSIKAQDLPAYIQGSEIGEKLIQYRIDALA
ELKKQYDDSLATE

Sequences:

>Translated_413_residues
MQVYLVGGAVRDHLLGHPYHEKDYVVVGASPQQLLDQGFSPVGKDFPVFLHPKTKEEYALARTERKSGIGYHGFNFFTDP
DVSLEDDLIRRDLTINAMAMDQDGNVYDPYGGQKDLEQRILRHVSDAFIEDPLRVLRVARFAARYARYGFKIADETLLLM
QSITQTGELESLTAERVWKETSRALMEDQADIYFDVLRQCHALKVLFPEIDALFGVPQRPEYHPEIDCGIHTLMSLRQAC
KSNYSLDVRYAVLVHDLGKALTPKDILPRHIMHEERGLIPVEELSNRLKIPTQMKQLALAVCKEHLKCHQAMSLKPGTLW
RLLQRLDVLRRPERVEAFINACECDARGRLGLENRDYPQAAFMFKAMQVVRSIKAQDLPAYIQGSEIGEKLIQYRIDALA
ELKKQYDDSLATE
>Mature_413_residues
MQVYLVGGAVRDHLLGHPYHEKDYVVVGASPQQLLDQGFSPVGKDFPVFLHPKTKEEYALARTERKSGIGYHGFNFFTDP
DVSLEDDLIRRDLTINAMAMDQDGNVYDPYGGQKDLEQRILRHVSDAFIEDPLRVLRVARFAARYARYGFKIADETLLLM
QSITQTGELESLTAERVWKETSRALMEDQADIYFDVLRQCHALKVLFPEIDALFGVPQRPEYHPEIDCGIHTLMSLRQAC
KSNYSLDVRYAVLVHDLGKALTPKDILPRHIMHEERGLIPVEELSNRLKIPTQMKQLALAVCKEHLKCHQAMSLKPGTLW
RLLQRLDVLRRPERVEAFINACECDARGRLGLENRDYPQAAFMFKAMQVVRSIKAQDLPAYIQGSEIGEKLIQYRIDALA
ELKKQYDDSLATE

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=406, Percent_Identity=55.6650246305419, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI87081691, Length=231, Percent_Identity=30.3030303030303, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71995920, Length=220, Percent_Identity=28.1818181818182, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_ACIAD (Q6FA38)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046897.1
- ProteinModelPortal:   Q6FA38
- SMR:   Q6FA38
- STRING:   Q6FA38
- GeneID:   2879929
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2288
- NMPDR:   fig|62977.3.peg.2184
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   RWACLLH
- PhylomeDB:   Q6FA38
- ProtClustDB:   PRK10885
- BioCyc:   ASP62977:ACIAD2288-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR002646
- PIRSF:   PIRSF000813

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 47269; Mature: 47269

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVYLVGGAVRDHLLGHPYHEKDYVVVGASPQQLLDQGFSPVGKDFPVFLHPKTKEEYAL
CEEEEECCHHHHHHCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKSGIGYHGFNFFTDPDVSLEDDLIRRDLTINAMAMDQDGNVYDPYGGQKDLEQRI
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEEECCCCCEECCCCCHHHHHHHH
LRHVSDAFIEDPLRVLRVARFAARYARYGFKIADETLLLMQSITQTGELESLTAERVWKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TSRALMEDQADIYFDVLRQCHALKVLFPEIDALFGVPQRPEYHPEIDCGIHTLMSLRQAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
KSNYSLDVRYAVLVHDLGKALTPKDILPRHIMHEERGLIPVEELSNRLKIPTQMKQLALA
HCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHH
VCKEHLKCHQAMSLKPGTLWRLLQRLDVLRRPERVEAFINACECDARGRLGLENRDYPQA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHH
AFMFKAMQVVRSIKAQDLPAYIQGSEIGEKLIQYRIDALAELKKQYDDSLATE
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQVYLVGGAVRDHLLGHPYHEKDYVVVGASPQQLLDQGFSPVGKDFPVFLHPKTKEEYAL
CEEEEECCHHHHHHCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKSGIGYHGFNFFTDPDVSLEDDLIRRDLTINAMAMDQDGNVYDPYGGQKDLEQRI
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEEECCCCCEECCCCCHHHHHHHH
LRHVSDAFIEDPLRVLRVARFAARYARYGFKIADETLLLMQSITQTGELESLTAERVWKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TSRALMEDQADIYFDVLRQCHALKVLFPEIDALFGVPQRPEYHPEIDCGIHTLMSLRQAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
KSNYSLDVRYAVLVHDLGKALTPKDILPRHIMHEERGLIPVEELSNRLKIPTQMKQLALA
HCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHH
VCKEHLKCHQAMSLKPGTLWRLLQRLDVLRRPERVEAFINACECDARGRLGLENRDYPQA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHH
AFMFKAMQVVRSIKAQDLPAYIQGSEIGEKLIQYRIDALAELKKQYDDSLATE
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA