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The map label for this gene is glnG [H]

Identifier: 50084547

GI number: 50084547

Start: 1363631

End: 1365106

Strand: Reverse

Name: glnG [H]

Synonym: ACIAD1368

Alternate gene names: 50084547

Gene position: 1365106-1363631 (Counterclockwise)

Preceding gene: 50084548

Following gene: 50084546

Centisome position: 37.93

GC content: 43.02

Gene sequence:

>1476_bases
ATGTCGCGAAATAAAATATGGGTAATTGATGATGATCGCGCCATGCGCTGGGTCTTGGAAAAAACCTTTAAAGAAGAAGG
CTTTGATGTTACAAGTTTCGAAGAGGCACAATCCGCGCTTGATCAGCTTTTGGACGATGCACCAGACGTAATCTTAACTG
ATATTCGTATGCCAGGTATCGATGGTTTAACTTTTCTAGGAAAAGTTAAAAATAACTATCCTGATTTGCCTGTCATCATC
ATGACTGCTCACTCAGATCTTGAATCCGCGGTCTCTAGCTATCAGACAGGTGCTTTTGAGTATTTACCTAAACCATTTGA
CATTGATGAAGCGCTGGCTTTAGTTAATCGTGCGATCCTGCATATTAATAAACTTCAACAGCAAGAAGCCACCAAAGCAG
CTTCTGCGGCACAATCAACTGAAATTATTGGTGAATCTCCTGCCATGCAGGAAGTATTCAGAGCGATTGGGCGTTTATCA
CAATCTCATATCACCGTACTTATTAATGGTGAATCAGGTACAGGTAAAGAACTGGTTGCGCATGCCTTACATAAGCATTC
TCCGCGCAGCAGTAAGCCGTTTATCGCGCTCAACATGGCAGCAATTCCAAAAGACCTGATTGAAACTGAACTGTTCGGTC
ACGAGAAAGGTGCTTTTACTGGTGCAAATACTCAACGTCAGGGTCGTTTTGAGCAAGCCAATGGCGGCACATTATTTTTA
GATGAAATTGGCGATATGCCTTTTGAAACACAAACACGTTTATTACGTGTATTGGCAGATGGTGAGTTTTATCGTGTAGG
TGGACATATTCCTGTTAAGGTTGATGTTCGAATTGTGGCTGCAACGCATCAAGATCTCGAAAAACTGGTGAATGAAGGCC
GATTCCGTGAAGACTTGTACCATCGTCTAAACGTGATCCGAATCCATATTCCGAAGCTCGCTCACCGTAGCGAAGACATC
CCAATGCTAGCACAACACTTTCTAGCACGCGCGGGCAAAGAACTGGGTGTAAGTCCTAAAATTTTGCGGACAGAAACCAC
TGATTATATGCAACAGCTCCCTTGGCCAGGGAATGTACGCCAACTAGAAAATACATGTCGATGGCTAACGGTAATGATTA
CGGGACGAGAAGTCTATCCTGAAGATCTCCCTTCAGAACTCAAACAGATTCCAATTCAGAAATCTGTGGATCAGACAGGT
ACACCGCCAAGCTTTGATCGTATTTCATTGCATCATTGGGATGAGCTACTTGGACAATGGGCTATTCAAAAACTTAAAAA
TGGTGAAATGAAAATTTTGGATATCGCCACTCCTATGTTTGAGCGTACTTTGATCAATGCTGCGCTTCAACAAACGCGTG
GACGCAAACGTCATGCAGCCGAACTTCTCGGTTGGGGACGCAATACACTTACTCGGAAATTAAAAGAACTTGGTATCAGC
GCAGAAGATGATACTGAAGAAGAAAGTGAAATTTAA

Upstream 100 bases:

>100_bases
ATTGCACAAAACATTATGCATCAACACAACGGGATGATTGAGTGCCAATCTATTCCAGGAAAAACAGTTTTTAGCTTATA
TCTACCTTGGGAGTCCGACC

Downstream 100 bases:

>100_bases
GTAATCGTGATGAGTAGAGTATTCATCATCAGAATGCTCTACTTAACAACAATAAAAAAATATTAAACAGCTTAAATTTT
CTGGCAAAGCATGAAACCTT

Product: two-component regulatory system response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSRNKIWVIDDDRAMRWVLEKTFKEEGFDVTSFEEAQSALDQLLDDAPDVILTDIRMPGIDGLTFLGKVKNNYPDLPVII
MTAHSDLESAVSSYQTGAFEYLPKPFDIDEALALVNRAILHINKLQQQEATKAASAAQSTEIIGESPAMQEVFRAIGRLS
QSHITVLINGESGTGKELVAHALHKHSPRSSKPFIALNMAAIPKDLIETELFGHEKGAFTGANTQRQGRFEQANGGTLFL
DEIGDMPFETQTRLLRVLADGEFYRVGGHIPVKVDVRIVAATHQDLEKLVNEGRFREDLYHRLNVIRIHIPKLAHRSEDI
PMLAQHFLARAGKELGVSPKILRTETTDYMQQLPWPGNVRQLENTCRWLTVMITGREVYPEDLPSELKQIPIQKSVDQTG
TPPSFDRISLHHWDELLGQWAIQKLKNGEMKILDIATPMFERTLINAALQQTRGRKRHAAELLGWGRNTLTRKLKELGIS
AEDDTEEESEI

Sequences:

>Translated_491_residues
MSRNKIWVIDDDRAMRWVLEKTFKEEGFDVTSFEEAQSALDQLLDDAPDVILTDIRMPGIDGLTFLGKVKNNYPDLPVII
MTAHSDLESAVSSYQTGAFEYLPKPFDIDEALALVNRAILHINKLQQQEATKAASAAQSTEIIGESPAMQEVFRAIGRLS
QSHITVLINGESGTGKELVAHALHKHSPRSSKPFIALNMAAIPKDLIETELFGHEKGAFTGANTQRQGRFEQANGGTLFL
DEIGDMPFETQTRLLRVLADGEFYRVGGHIPVKVDVRIVAATHQDLEKLVNEGRFREDLYHRLNVIRIHIPKLAHRSEDI
PMLAQHFLARAGKELGVSPKILRTETTDYMQQLPWPGNVRQLENTCRWLTVMITGREVYPEDLPSELKQIPIQKSVDQTG
TPPSFDRISLHHWDELLGQWAIQKLKNGEMKILDIATPMFERTLINAALQQTRGRKRHAAELLGWGRNTLTRKLKELGIS
AEDDTEEESEI
>Mature_490_residues
SRNKIWVIDDDRAMRWVLEKTFKEEGFDVTSFEEAQSALDQLLDDAPDVILTDIRMPGIDGLTFLGKVKNNYPDLPVIIM
TAHSDLESAVSSYQTGAFEYLPKPFDIDEALALVNRAILHINKLQQQEATKAASAAQSTEIIGESPAMQEVFRAIGRLSQ
SHITVLINGESGTGKELVAHALHKHSPRSSKPFIALNMAAIPKDLIETELFGHEKGAFTGANTQRQGRFEQANGGTLFLD
EIGDMPFETQTRLLRVLADGEFYRVGGHIPVKVDVRIVAATHQDLEKLVNEGRFREDLYHRLNVIRIHIPKLAHRSEDIP
MLAQHFLARAGKELGVSPKILRTETTDYMQQLPWPGNVRQLENTCRWLTVMITGREVYPEDLPSELKQIPIQKSVDQTGT
PPSFDRISLHHWDELLGQWAIQKLKNGEMKILDIATPMFERTLINAALQQTRGRKRHAAELLGWGRNTLTRKLKELGISA
EDDTEEESEI

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=479, Percent_Identity=64.0918580375783, Blast_Score=628, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=487, Percent_Identity=40.8624229979466, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1790437, Length=470, Percent_Identity=41.9148936170213, Blast_Score=351, Evalue=6e-98,
Organism=Escherichia coli, GI1788905, Length=484, Percent_Identity=36.5702479338843, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI1789087, Length=339, Percent_Identity=42.1828908554572, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI87082117, Length=349, Percent_Identity=39.541547277937, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1789233, Length=340, Percent_Identity=38.8235294117647, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082152, Length=273, Percent_Identity=43.5897435897436, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1787583, Length=370, Percent_Identity=35.9459459459459, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1786524, Length=348, Percent_Identity=39.367816091954, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI87081872, Length=344, Percent_Identity=37.2093023255814, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789828, Length=225, Percent_Identity=36, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87081858, Length=324, Percent_Identity=30.5555555555556, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789809, Length=181, Percent_Identity=30.939226519337, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1790860, Length=125, Percent_Identity=29.6, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786599, Length=134, Percent_Identity=30.5970149253731, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787375, Length=109, Percent_Identity=29.3577981651376, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55328; Mature: 55197

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNKIWVIDDDRAMRWVLEKTFKEEGFDVTSFEEAQSALDQLLDDAPDVILTDIRMPGI
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
DGLTFLGKVKNNYPDLPVIIMTAHSDLESAVSSYQTGAFEYLPKPFDIDEALALVNRAIL
CCHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHH
HINKLQQQEATKAASAAQSTEIIGESPAMQEVFRAIGRLSQSHITVLINGESGTGKELVA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
HALHKHSPRSSKPFIALNMAAIPKDLIETELFGHEKGAFTGANTQRQGRFEQANGGTLFL
HHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEH
DEIGDMPFETQTRLLRVLADGEFYRVGGHIPVKVDVRIVAATHQDLEKLVNEGRFREDLY
HHHCCCCCHHHHHHHHHHHCCCEEEECCEEEEEEEEEEEECCHHHHHHHHHCCCHHHHHH
HRLNVIRIHIPKLAHRSEDIPMLAQHFLARAGKELGVSPKILRTETTDYMQQLPWPGNVR
HHHEEEEEECCHHHCCCCCCHHHHHHHHHHCCHHCCCCCCEEECCCHHHHHHCCCCCCHH
QLENTCRWLTVMITGREVYPEDLPSELKQIPIQKSVDQTGTPPSFDRISLHHWDELLGQW
HHHHHHEEEEEEEECCCCCCHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHH
AIQKLKNGEMKILDIATPMFERTLINAALQQTRGRKRHAAELLGWGRNTLTRKLKELGIS
HHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCC
AEDDTEEESEI
CCCCCCHHCCC
>Mature Secondary Structure 
SRNKIWVIDDDRAMRWVLEKTFKEEGFDVTSFEEAQSALDQLLDDAPDVILTDIRMPGI
CCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
DGLTFLGKVKNNYPDLPVIIMTAHSDLESAVSSYQTGAFEYLPKPFDIDEALALVNRAIL
CCHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHH
HINKLQQQEATKAASAAQSTEIIGESPAMQEVFRAIGRLSQSHITVLINGESGTGKELVA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
HALHKHSPRSSKPFIALNMAAIPKDLIETELFGHEKGAFTGANTQRQGRFEQANGGTLFL
HHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEH
DEIGDMPFETQTRLLRVLADGEFYRVGGHIPVKVDVRIVAATHQDLEKLVNEGRFREDLY
HHHCCCCCHHHHHHHHHHHCCCEEEECCEEEEEEEEEEEECCHHHHHHHHHCCCHHHHHH
HRLNVIRIHIPKLAHRSEDIPMLAQHFLARAGKELGVSPKILRTETTDYMQQLPWPGNVR
HHHEEEEEECCHHHCCCCCCHHHHHHHHHHCCHHCCCCCCEEECCCHHHHHHCCCCCCHH
QLENTCRWLTVMITGREVYPEDLPSELKQIPIQKSVDQTGTPPSFDRISLHHWDELLGQW
HHHHHHEEEEEEEECCCCCCHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHH
AIQKLKNGEMKILDIATPMFERTLINAALQQTRGRKRHAAELLGWGRNTLTRKLKELGIS
HHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCC
AEDDTEEESEI
CCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]