Click here to switch to the map view.

The map label for this gene is glnL [H]

Identifier: 50084548

GI number: 50084548

Start: 1365093

End: 1366202

Strand: Reverse

Name: glnL [H]

Synonym: ACIAD1369

Alternate gene names: 50084548

Gene position: 1366202-1365093 (Counterclockwise)

Preceding gene: 50084561

Following gene: 50084547

Centisome position: 37.96

GC content: 40.63

Gene sequence:

>1110_bases
ATGGATCAACAACCGACTATCGACTATCGACTACTCGTAGATAACATGACTACTGCCATTTTACTGGTCGATAGTAACTT
GAACATTTTTTATCTTAATTCATCCTGTGAAGCATTATTTGACATCAGTTTACTTCGTGCATCTGGACAGCCTGTAATCA
ATCTACTGCATGCTCCAAACGATACCTTTAACACCCACGAAGCCTTACTTAATACACTTAAGTCCAGTCAGCATTATACA
CGTCGTGAAGCCACTATTAATGTGAACTTCAAAGACATTCATGTAGATTATACCGTTTCTCAACTCAACACAGGCAAACC
TTACCACCCGCTGTTATTGATCGAACTCAATCCAATTGATCGAATGCTTAAAATTTCTAAAGAAGAAAATTTTATTCAGC
AGCACCAAGTCGCCAGACAGCTCATCCGTGGTGTAGCACACGAGATTAAAAATCCACTTGGTGGTATACGTGGTGCAACA
CAATTGCTTGCCCGTAGTCTAAATGATCCGAAATATGCAGAATTCACAGACATTATTATCAATGAGGTAGATCGTCTAAG
GAATCTGGCAGACACGATGCTCGGTTCAAGACAGTTGCCAAGCTACGAAGACGTGAATATTCATGAACCTCTGGAACGTG
TTCGTTCACTGATTGTTAATCAGACCAAGAAGAAAATTAAGATTATTCGTGATTACGACTTATCTCTACCCGATGTAAAA
GCTGATCGAGATCAGCTCATACAAGTCATGCTCAACATTAGTGTCAATGCAATCCAAGCCATTACCGAAAATAAAGAGTT
TTTTGTTGAACATGAACCTCAACTTATGCTCCGTACCCGTATCCAGCGTCTAGTCACCATCAATGGTGTACTTAACCGCT
CAGCTGTACGAATTGATATTGAAGATAACGGCCCAGGTATTCCAGAGGATATTTTAGAATCCGTCTTTTATCCATTGGTT
ACGGGCCGAGCAAAAGGCACAGGGCTGGGCTTAAGTATTGCACAAAACATTATGCATCAACACAACGGGATGATTGAGTG
CCAATCTATTCCAGGAAAAACAGTTTTTAGCTTATATCTACCTTGGGAGTCCGACCATGTCGCGAAATAA

Upstream 100 bases:

>100_bases
AAAGTTGGCGTGTATTTTGCTTCTCATATGTCCCATAACAGGGATAGATTTTGTTAAATCAGATGCCATTGCGCTATTTT
AAAACAGGAACCTTCCGCTA

Downstream 100 bases:

>100_bases
AATATGGGTAATTGATGATGATCGCGCCATGCGCTGGGTCTTGGAAAAAACCTTTAAAGAAGAAGGCTTTGATGTTACAA
GTTTCGAAGAGGCACAATCC

Product: sensory kinase (soluble) in two-component regulatory system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MDQQPTIDYRLLVDNMTTAILLVDSNLNIFYLNSSCEALFDISLLRASGQPVINLLHAPNDTFNTHEALLNTLKSSQHYT
RREATINVNFKDIHVDYTVSQLNTGKPYHPLLLIELNPIDRMLKISKEENFIQQHQVARQLIRGVAHEIKNPLGGIRGAT
QLLARSLNDPKYAEFTDIIINEVDRLRNLADTMLGSRQLPSYEDVNIHEPLERVRSLIVNQTKKKIKIIRDYDLSLPDVK
ADRDQLIQVMLNISVNAIQAITENKEFFVEHEPQLMLRTRIQRLVTINGVLNRSAVRIDIEDNGPGIPEDILESVFYPLV
TGRAKGTGLGLSIAQNIMHQHNGMIECQSIPGKTVFSLYLPWESDHVAK

Sequences:

>Translated_369_residues
MDQQPTIDYRLLVDNMTTAILLVDSNLNIFYLNSSCEALFDISLLRASGQPVINLLHAPNDTFNTHEALLNTLKSSQHYT
RREATINVNFKDIHVDYTVSQLNTGKPYHPLLLIELNPIDRMLKISKEENFIQQHQVARQLIRGVAHEIKNPLGGIRGAT
QLLARSLNDPKYAEFTDIIINEVDRLRNLADTMLGSRQLPSYEDVNIHEPLERVRSLIVNQTKKKIKIIRDYDLSLPDVK
ADRDQLIQVMLNISVNAIQAITENKEFFVEHEPQLMLRTRIQRLVTINGVLNRSAVRIDIEDNGPGIPEDILESVFYPLV
TGRAKGTGLGLSIAQNIMHQHNGMIECQSIPGKTVFSLYLPWESDHVAK
>Mature_369_residues
MDQQPTIDYRLLVDNMTTAILLVDSNLNIFYLNSSCEALFDISLLRASGQPVINLLHAPNDTFNTHEALLNTLKSSQHYT
RREATINVNFKDIHVDYTVSQLNTGKPYHPLLLIELNPIDRMLKISKEENFIQQHQVARQLIRGVAHEIKNPLGGIRGAT
QLLARSLNDPKYAEFTDIIINEVDRLRNLADTMLGSRQLPSYEDVNIHEPLERVRSLIVNQTKKKIKIIRDYDLSLPDVK
ADRDQLIQVMLNISVNAIQAITENKEFFVEHEPQLMLRTRIQRLVTINGVLNRSAVRIDIEDNGPGIPEDILESVFYPLV
TGRAKGTGLGLSIAQNIMHQHNGMIECQSIPGKTVFSLYLPWESDHVAK

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=356, Percent_Identity=41.2921348314607, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1788549, Length=361, Percent_Identity=27.9778393351801, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=30.8370044052863, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41910; Mature: 41910

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQQPTIDYRLLVDNMTTAILLVDSNLNIFYLNSSCEALFDISLLRASGQPVINLLHAPN
CCCCCCCEEEEEECCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCC
DTFNTHEALLNTLKSSQHYTRREATINVNFKDIHVDYTVSQLNTGKPYHPLLLIELNPID
CCCCHHHHHHHHHHCCCHHHHCCEEEEEEEEEEEEEEEEEECCCCCCCCCEEEEEECCHH
RMLKISKEENFIQQHQVARQLIRGVAHEIKNPLGGIRGATQLLARSLNDPKYAEFTDIII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHH
NEVDRLRNLADTMLGSRQLPSYEDVNIHEPLERVRSLIVNQTKKKIKIIRDYDLSLPDVK
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCCCC
ADRDQLIQVMLNISVNAIQAITENKEFFVEHEPQLMLRTRIQRLVTINGVLNRSAVRIDI
CCHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHEECCCEEEEEE
EDNGPGIPEDILESVFYPLVTGRAKGTGLGLSIAQNIMHQHNGMIECQSIPGKTVFSLYL
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEEE
PWESDHVAK
CCCCCCCCC
>Mature Secondary Structure
MDQQPTIDYRLLVDNMTTAILLVDSNLNIFYLNSSCEALFDISLLRASGQPVINLLHAPN
CCCCCCCEEEEEECCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCC
DTFNTHEALLNTLKSSQHYTRREATINVNFKDIHVDYTVSQLNTGKPYHPLLLIELNPID
CCCCHHHHHHHHHHCCCHHHHCCEEEEEEEEEEEEEEEEEECCCCCCCCCEEEEEECCHH
RMLKISKEENFIQQHQVARQLIRGVAHEIKNPLGGIRGATQLLARSLNDPKYAEFTDIII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHH
NEVDRLRNLADTMLGSRQLPSYEDVNIHEPLERVRSLIVNQTKKKIKIIRDYDLSLPDVK
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCCCC
ADRDQLIQVMLNISVNAIQAITENKEFFVEHEPQLMLRTRIQRLVTINGVLNRSAVRIDI
CCHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHEECCCEEEEEE
EDNGPGIPEDILESVFYPLVTGRAKGTGLGLSIAQNIMHQHNGMIECQSIPGKTVFSLYL
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEEE
PWESDHVAK
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]