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The map label for this gene is cfxR [H]

Identifier: 50084483

GI number: 50084483

Start: 1302679

End: 1303695

Strand: Direct

Name: cfxR [H]

Synonym: ACIAD1300

Alternate gene names: 50084483

Gene position: 1302679-1303695 (Clockwise)

Preceding gene: 50084482

Following gene: 50084484

Centisome position: 36.2

GC content: 35.89

Gene sequence:

>1017_bases
ATGTTTGCTCTTAAAAATATGGAGAAAACAACAGTGTTGAATCGCATATCTTTCAGACAAATGGAATATTTTGTTTCAAC
TGCACAGCATGGCAGTATTATTGTGGCATCGGAGAAAATACATGTTTCTCCACCTTCAATTTCTGCTGCAATTTCCCATA
TTGAATCAGAATTAAAAGTGAAATTATTTATTCGTCAACACGCGCGTGGCTTATCCTTGACCAATATCGGTGAAAAAATT
CTTAAAGAATGTTTGTTTATTTTGGGGCAAACCTATGCACTTTATGCACTTGCTTCTGAATATCGCGGTTTGACACAACG
AGCCTTACAAGTGGGTTGTCATCATGTTTTAACGCCGTTGATTTATTCAGATATTGTGCATGATTTTAATCTTTTACATC
AACAAGATGATATTCGCTTAGTGGATGGTAGCCATGACGAACTCATGAATGCTTTACTCTCAAATAAAGTAGACATGGCA
ATCACCACAAATTTACAGATTGATCATAACCTTATTTGTTTTGAGCCGATAGTGAGTTTGCCTCCTCATGTGTTGGTGAG
TGAACATCATCCATTGGCAAAAATGAAAAGTGTTCGCTTAGAAGATTTAACTCAGTTTCCGATGATTTTGCACGACTCTC
CCTATTATAAAGATTATTGCACTGATTTATTTACACAGCATAACTTGGTGCCACATATCAAAGATACCTACAAAAACTAT
GATCTAGTACGAACCATGGTGGCTGATGGATGGGGTTACAGCATGACCAATTTACGACATAAGCTTGAGCATACATTAAA
TGGTAAAAAACTCATTCGCATAATGTTAGAAGGAGATCATGCACCAGTAAGTATAGGAATTGCCACAATCTCCAGTGTCA
ATTCGAATGAAGTTACAGAAAAATTTATGAGCTACTGTAAAGATTTTATTTGTGAGTATTATCATGATCATGCTTCATGC
AGTTTCACTTTAACTTCTTCAATTAAAAAAACGCGATCTATACCAAGCGTGGCTTAA

Upstream 100 bases:

>100_bases
TGATGAAAATTGATTGTAGTTAAGGTCAATCATCTTGGCGTTTTAAATGAAGCATAAATAGTAGATTACTTTATTTTCTT
ATGAGTTGAATTTTCTATTT

Downstream 100 bases:

>100_bases
AAAAATCATCCGTTAGCGATCAGTATTGATCATTTAACTGATGTTTTAAATTATTAAAATTGAGATGAGTGATATGCAGC
ACGTCAGAATAACATGTACT

Product: transcriptional regulator

Products: NA

Alternate protein names: RuBisCO operon transcriptional regulator [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MFALKNMEKTTVLNRISFRQMEYFVSTAQHGSIIVASEKIHVSPPSISAAISHIESELKVKLFIRQHARGLSLTNIGEKI
LKECLFILGQTYALYALASEYRGLTQRALQVGCHHVLTPLIYSDIVHDFNLLHQQDDIRLVDGSHDELMNALLSNKVDMA
ITTNLQIDHNLICFEPIVSLPPHVLVSEHHPLAKMKSVRLEDLTQFPMILHDSPYYKDYCTDLFTQHNLVPHIKDTYKNY
DLVRTMVADGWGYSMTNLRHKLEHTLNGKKLIRIMLEGDHAPVSIGIATISSVNSNEVTEKFMSYCKDFICEYYHDHASC
SFTLTSSIKKTRSIPSVA

Sequences:

>Translated_338_residues
MFALKNMEKTTVLNRISFRQMEYFVSTAQHGSIIVASEKIHVSPPSISAAISHIESELKVKLFIRQHARGLSLTNIGEKI
LKECLFILGQTYALYALASEYRGLTQRALQVGCHHVLTPLIYSDIVHDFNLLHQQDDIRLVDGSHDELMNALLSNKVDMA
ITTNLQIDHNLICFEPIVSLPPHVLVSEHHPLAKMKSVRLEDLTQFPMILHDSPYYKDYCTDLFTQHNLVPHIKDTYKNY
DLVRTMVADGWGYSMTNLRHKLEHTLNGKKLIRIMLEGDHAPVSIGIATISSVNSNEVTEKFMSYCKDFICEYYHDHASC
SFTLTSSIKKTRSIPSVA
>Mature_338_residues
MFALKNMEKTTVLNRISFRQMEYFVSTAQHGSIIVASEKIHVSPPSISAAISHIESELKVKLFIRQHARGLSLTNIGEKI
LKECLFILGQTYALYALASEYRGLTQRALQVGCHHVLTPLIYSDIVHDFNLLHQQDDIRLVDGSHDELMNALLSNKVDMA
ITTNLQIDHNLICFEPIVSLPPHVLVSEHHPLAKMKSVRLEDLTQFPMILHDSPYYKDYCTDLFTQHNLVPHIKDTYKNY
DLVRTMVADGWGYSMTNLRHKLEHTLNGKKLIRIMLEGDHAPVSIGIATISSVNSNEVTEKFMSYCKDFICEYYHDHASC
SFTLTSSIKKTRSIPSVA

Specific function: Trans-acting transcriptional regulator of RuBisCO genes (cfxLS) expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=244, Percent_Identity=23.3606557377049, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788296, Length=243, Percent_Identity=23.0452674897119, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787601, Length=237, Percent_Identity=24.0506329113924, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693105, Length=218, Percent_Identity=24.3119266055046, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=192, Percent_Identity=26.0416666666667, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790399, Length=248, Percent_Identity=25, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 38427; Mature: 38427

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFALKNMEKTTVLNRISFRQMEYFVSTAQHGSIIVASEKIHVSPPSISAAISHIESELKV
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECEEEECCCHHHHHHHHHHHHHHH
KLFIRQHARGLSLTNIGEKILKECLFILGQTYALYALASEYRGLTQRALQVGCHHVLTPL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYSDIVHDFNLLHQQDDIRLVDGSHDELMNALLSNKVDMAITTNLQIDHNLICFEPIVSL
HHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCEEEEECEEECCCEEEECHHHCC
PPHVLVSEHHPLAKMKSVRLEDLTQFPMILHDSPYYKDYCTDLFTQHNLVPHIKDTYKNY
CCCEEECCCCCHHHHHHCCHHHHHHCCHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
DLVRTMVADGWGYSMTNLRHKLEHTLNGKKLIRIMLEGDHAPVSIGIATISSVNSNEVTE
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHEEEEEEECCCCCEEEEEEECCCCCCHHHHH
KFMSYCKDFICEYYHDHASCSFTLTSSIKKTRSIPSVA
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCC
>Mature Secondary Structure
MFALKNMEKTTVLNRISFRQMEYFVSTAQHGSIIVASEKIHVSPPSISAAISHIESELKV
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECEEEECCCHHHHHHHHHHHHHHH
KLFIRQHARGLSLTNIGEKILKECLFILGQTYALYALASEYRGLTQRALQVGCHHVLTPL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYSDIVHDFNLLHQQDDIRLVDGSHDELMNALLSNKVDMAITTNLQIDHNLICFEPIVSL
HHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCEEEEECEEECCCEEEECHHHCC
PPHVLVSEHHPLAKMKSVRLEDLTQFPMILHDSPYYKDYCTDLFTQHNLVPHIKDTYKNY
CCCEEECCCCCHHHHHHCCHHHHHHCCHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
DLVRTMVADGWGYSMTNLRHKLEHTLNGKKLIRIMLEGDHAPVSIGIATISSVNSNEVTE
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHEEEEEEECCCCCEEEEEEECCCCCCHHHHH
KFMSYCKDFICEYYHDHASCSFTLTSSIKKTRSIPSVA
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1779759 [H]