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The map label for this gene is yiaE [C]

Identifier: 50084484

GI number: 50084484

Start: 1303769

End: 1304728

Strand: Direct

Name: yiaE [C]

Synonym: ACIAD1301

Alternate gene names: 50084484

Gene position: 1303769-1304728 (Clockwise)

Preceding gene: 50084483

Following gene: 50084486

Centisome position: 36.23

GC content: 37.6

Gene sequence:

>960_bases
ATGCAGCACGTCAGAATAACATGTACTGAAAAACAGTATTTGGGGAATCAAACGTTCAGATTTAATTTTCCTGTGAACTA
CACCGAATTTGAAAAGTTAACCCAACAGCAATTTTATGATCAAGTGAAGGATCAGCATGTTTTAATCGTAAATGATTTAA
AAGTTGATCAGCAAGTGATGGATAACAATCCAGAGCTTCAATTGATTGCACTCTGTTCGACTGGTTTTGATCATGTCGAT
ATTGGGTTAGCAAAACAGAGAAATATAAAAGTGTGTAATATTAAGGGATATGCAGGCGATACCGTCGCCGAACATGCTTT
TTTACTCATGCTGAATTTGGTTAAAAATTTGCAGTTTTATCAGAATTCAGTTGTTTCAGGATTATGGAGTGACAGCAATT
GTTTTTGCTATCAAGATCATCACTTTCCAATTTATGATCTTAAAGACAAAACATTGGTGATTGTAGGTCGAGGTGAAATA
GGACAATCCTTGGCATTAAAAGCACAAGCGTTTGGAATGCAGGTATTTTTTTCTGAACGCCCGAATCAAGAAGGCTGTAG
AGAGGGATATATTCCATTTCAGACAGCACTTCAAATTGCAGATGTGCTGTCATTGCATTGTTCACTAAACGCTGACAATC
GGCATATGCTTAATCAAGAAACACTAAATTTAATGAAACCGAATAGTTTTATTATAAACGTCAGTCGAGGTGGGTTAGTA
AATGAGCACGATTTACTCATTGCACTTCGTACAGGCCGCATTGCGGGCTATGGAGCAGATGCCATGACCCAAGAACCTCC
TCCTAGAGATAATCCACTTTTAAAGAGTGGATTACCCAATATATTGCTTACACCGCATATCGCATGGGCAAGCGAAGAGG
CGAAACAGCGTATGATTGAGATACTGGTTCAGAATATTCATTTAAATTTAGATGGAATAGATCACAATAGAATCGTTTAG

Upstream 100 bases:

>100_bases
ACGCGATCTATACCAAGCGTGGCTTAAAAAAATCATCCGTTAGCGATCAGTATTGATCATTTAACTGATGTTTTAAATTA
TTAAAATTGAGATGAGTGAT

Downstream 100 bases:

>100_bases
CAGTGTTGGACATCTTGATCAGCGAGTTGCAAATCTGCATGTAGATGAGGAAAGTTGATTAAGCGTAGAAAATCACCACG
CTGTAAAAAAATAAAAATCG

Product: glycerate dehydrogenase

Products: hydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MQHVRITCTEKQYLGNQTFRFNFPVNYTEFEKLTQQQFYDQVKDQHVLIVNDLKVDQQVMDNNPELQLIALCSTGFDHVD
IGLAKQRNIKVCNIKGYAGDTVAEHAFLLMLNLVKNLQFYQNSVVSGLWSDSNCFCYQDHHFPIYDLKDKTLVIVGRGEI
GQSLALKAQAFGMQVFFSERPNQEGCREGYIPFQTALQIADVLSLHCSLNADNRHMLNQETLNLMKPNSFIINVSRGGLV
NEHDLLIALRTGRIAGYGADAMTQEPPPRDNPLLKSGLPNILLTPHIAWASEEAKQRMIEILVQNIHLNLDGIDHNRIV

Sequences:

>Translated_319_residues
MQHVRITCTEKQYLGNQTFRFNFPVNYTEFEKLTQQQFYDQVKDQHVLIVNDLKVDQQVMDNNPELQLIALCSTGFDHVD
IGLAKQRNIKVCNIKGYAGDTVAEHAFLLMLNLVKNLQFYQNSVVSGLWSDSNCFCYQDHHFPIYDLKDKTLVIVGRGEI
GQSLALKAQAFGMQVFFSERPNQEGCREGYIPFQTALQIADVLSLHCSLNADNRHMLNQETLNLMKPNSFIINVSRGGLV
NEHDLLIALRTGRIAGYGADAMTQEPPPRDNPLLKSGLPNILLTPHIAWASEEAKQRMIEILVQNIHLNLDGIDHNRIV
>Mature_319_residues
MQHVRITCTEKQYLGNQTFRFNFPVNYTEFEKLTQQQFYDQVKDQHVLIVNDLKVDQQVMDNNPELQLIALCSTGFDHVD
IGLAKQRNIKVCNIKGYAGDTVAEHAFLLMLNLVKNLQFYQNSVVSGLWSDSNCFCYQDHHFPIYDLKDKTLVIVGRGEI
GQSLALKAQAFGMQVFFSERPNQEGCREGYIPFQTALQIADVLSLHCSLNADNRHMLNQETLNLMKPNSFIINVSRGGLV
NEHDLLIALRTGRIAGYGADAMTQEPPPRDNPLLKSGLPNILLTPHIAWASEEAKQRMIEILVQNIHLNLDGIDHNRIV

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580575, Length=286, Percent_Identity=30.0699300699301, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI61743967, Length=286, Percent_Identity=29.7202797202797, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI4557497, Length=286, Percent_Identity=29.7202797202797, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI145580578, Length=286, Percent_Identity=30.0699300699301, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI4557499, Length=286, Percent_Identity=30.0699300699301, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI6912396, Length=264, Percent_Identity=31.0606060606061, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI23308577, Length=288, Percent_Identity=28.4722222222222, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI87082289, Length=273, Percent_Identity=28.9377289377289, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1787645, Length=314, Percent_Identity=27.3885350318471, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789279, Length=284, Percent_Identity=27.4647887323944, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87081824, Length=199, Percent_Identity=25.1256281407035, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788660, Length=263, Percent_Identity=25.4752851711027, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI25147481, Length=271, Percent_Identity=26.9372693726937, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17532191, Length=250, Percent_Identity=30.4, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6324055, Length=296, Percent_Identity=28.0405405405405, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6324964, Length=283, Percent_Identity=26.1484098939929, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6320925, Length=289, Percent_Identity=27.681660899654, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322116, Length=289, Percent_Identity=26.9896193771626, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324980, Length=185, Percent_Identity=28.1081081081081, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24646446, Length=286, Percent_Identity=29.3706293706294, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24646448, Length=286, Percent_Identity=29.3706293706294, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24646452, Length=286, Percent_Identity=29.3706293706294, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24646450, Length=286, Percent_Identity=29.3706293706294, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI62472511, Length=286, Percent_Identity=29.3706293706294, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28571528, Length=267, Percent_Identity=31.8352059925094, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19921140, Length=312, Percent_Identity=26.6025641025641, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24585516, Length=252, Percent_Identity=27.7777777777778, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI28574286, Length=288, Percent_Identity=24.6527777777778, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI45552429, Length=256, Percent_Identity=26.953125, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24585514, Length=257, Percent_Identity=26.8482490272374, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28574282, Length=257, Percent_Identity=26.8482490272374, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28574284, Length=256, Percent_Identity=26.953125, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45551003, Length=256, Percent_Identity=26.953125, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.29

Molecular weight: Translated: 36222; Mature: 36222

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3 ; PS00850 GLY_RADICAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHVRITCTEKQYLGNQTFRFNFPVNYTEFEKLTQQQFYDQVKDQHVLIVNDLKVDQQVM
CCCEEEEECCHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHH
DNNPELQLIALCSTGFDHVDIGLAKQRNIKVCNIKGYAGDTVAEHAFLLMLNLVKNLQFY
CCCCCEEEEEEECCCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
QNSVVSGLWSDSNCFCYQDHHFPIYDLKDKTLVIVGRGEIGQSLALKAQAFGMQVFFSER
HHHHHHCCCCCCCEEEEECCCCCEEECCCCEEEEEECCCCCCHHEEEHHHHCEEEEECCC
PNQEGCREGYIPFQTALQIADVLSLHCSLNADNRHMLNQETLNLMKPNSFIINVSRGGLV
CCCCCHHCCCCCHHHHHHHHHHHHEEEECCCCCCHHCCHHHHHEECCCCEEEECCCCCCC
NEHDLLIALRTGRIAGYGADAMTQEPPPRDNPLLKSGLPNILLTPHIAWASEEAKQRMIE
CCCCEEEEEECCCEEECCCCCCCCCCCCCCCCHHHHCCCCEEECCCEECCCHHHHHHHHH
ILVQNIHLNLDGIDHNRIV
HHHHHHEEEECCCCCCCCC
>Mature Secondary Structure
MQHVRITCTEKQYLGNQTFRFNFPVNYTEFEKLTQQQFYDQVKDQHVLIVNDLKVDQQVM
CCCEEEEECCHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHH
DNNPELQLIALCSTGFDHVDIGLAKQRNIKVCNIKGYAGDTVAEHAFLLMLNLVKNLQFY
CCCCCEEEEEEECCCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
QNSVVSGLWSDSNCFCYQDHHFPIYDLKDKTLVIVGRGEIGQSLALKAQAFGMQVFFSER
HHHHHHCCCCCCCEEEEECCCCCEEECCCCEEEEEECCCCCCHHEEEHHHHCEEEEECCC
PNQEGCREGYIPFQTALQIADVLSLHCSLNADNRHMLNQETLNLMKPNSFIINVSRGGLV
CCCCCHHCCCCCHHHHHHHHHHHHEEEECCCCCCHHCCHHHHHEECCCCEEEECCCCCCC
NEHDLLIALRTGRIAGYGADAMTQEPPPRDNPLLKSGLPNILLTPHIAWASEEAKQRMIE
CCCCEEEEEECCCEEECCCCCCCCCCCCCCCCHHHHCCCCEEECCCEECCCHHHHHHHHH
ILVQNIHLNLDGIDHNRIV
HHHHHHEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-glycerate; NAD+

Specific reaction: (R)-glycerate + NAD+ = hydroxypyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]