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The map label for this gene is rlmL

Identifier: 50084454

GI number: 50084454

Start: 1268101

End: 1270305

Strand: Reverse

Name: rlmL

Synonym: ACIAD1268

Alternate gene names: 50084454

Gene position: 1270305-1268101 (Counterclockwise)

Preceding gene: 50084468

Following gene: 50084448

Centisome position: 35.3

GC content: 42.63

Gene sequence:

>2205_bases
ATGAACTCACCACAACGTCTTTCTACATACTGGGTAACCTGTGCGGATGGACTGGAAACCTTACTGCAACAAGAACTACA
AGGTTTAGGCATTGAGCAGACCGAACGTTTTGCTGGTCGTCTGATTTTCCAAGGGTCGCTTGAACAAGCCTATCGTGTCT
GCATGTGGTCGCGTTTGGCATCTCGTGTGCTGAAACCGATTCATACTTTTGAGCTTGAACGCACACACGATGCACGAGAT
GTTGCAGAAGAACTGTATGAAGGTGCGTTAAGTTTTGACTGGTCTCTGATTTTTGCACCACAAAGTACCTTTGCGATTCG
TTTGCATGTCGAACGCGAAATTAAGGTCAACTCACAATTTGCTACTTTGCGTGTAAAAGATGGTGTGGTCGATTCCTTTA
TGGAAGCCGTTGGCCGTCGTCCTAGCATTGATACGAAACAACCTGAAATTACCCTATTTGTGTTGGCAGGCAAAACCGAA
CATACCTATTGCCTTGATTTATCGGGTGATTCATTGCATAAACGTGGCTATCGTCATTATATGACAGATGCTCCGATCAA
AGAAAACCTAGCAGCAGCAATTTTGCAAAAAGCGAAACTCACAGAATTAAAGCCTGATCTTATTCTCGATCCAATGTGTG
GCTCTGGTACATTTATCATTGAATCACTGATGATTTTTACAGATCGTGCACCAGGATTAGTTCGTCGCTTTGGCTTTAAC
GGCTGGCATGGTCATGACCGCGAGCTCTGGCTTGAATTAAAAGCAGAAGCTGCCGAGCGCCATGAAATCGCTTTGCAAAA
CACCTTACCAAAATTTTATGCATTTGATGCAGACTGGGAAGCAGTTAAAGCAACTCGTCAAAATATTATTGCTGCTGGAT
TCGAAAAACTTCTAGACCAGATTCAGATTGAAGAGCGTACACTGGCAGATTGGCCATCATTTGAAAATACTTATAACACT
GCATTTGTTGTAACCAATCCACCTTATGGTGAGCGTTTAGGTGATAAGGCATCCAACCGTGCACTTTATCTAGGATTATC
AGCACTCTTACAACAGTATTTTCCGAAACAACATGCAGCAGTGATTGCAGCGCAAATTGAACAAGCGGATGTATTGGCAT
TCAGTGAGCCTGAAACTTTGCGTTTGATGAACGGTAAATTGCCAATTTATATTCGTCTGGGTCAAGTTAAGCCTGTCGCA
AATAGCCAACCATTTTTAGCAAACTGGCATGCCGATCCTGTCGAAATTGAAGGTGCACAGGATTTTGCCAACCGTTTACA
AAAAAATATGTCAGCTTTGAAAAAATGGGCGGTCAAAGATCAAGTATTCTGCTTGCGTTTATACGATGCAGATTTGCCAG
ATTTTAATGTTGCAGTCGACCTATATGGTGACCGTTTGCATGTTCAGGAATATGCACCACCCAAAACCATTGATCCAGAA
AAAGCCAAGAAACGTTTTAATTTGGCTTTGGCTGCAATTCGCGCCGTCACAGGTTTGCCTCGTGAGGCGATTTTTATTAA
AACACGTGCGCGCCAGGAAGGAAAAAACCAGTACACCAAACAGAGTACAGCGTCAAAGCGCTTTATCGTGCAGGAAGGTC
AAGCTAAAATTTTAGTCAATCTGACTGACTATCTGGATACAGGGCTATTTCTAGATCATCGCCAGATACGTTTACGTATT
GCTCAAGAAGCACGCGGCAAGCATTTTCTAAACCTGTTTAGTTATACCTCCACAGCCAGTCTGCATGCGGCACTGGGTGG
AGCTGCAAGTACCACCAGTGTAGATCTGTCAAACACCTACATCAACTGGTCCAAAGAGAACTTTGTCTTAAATGGTTTAA
CTGTAGATCATGCCGATGAACAACATATGTTCTTTGCCAGTGACTGCTTTGAGTGGTTAAAAGAAGGTCATGAGCAATAT
GATTTGATCTTTATTGACCCACCCACCTTTTCGAATTCAAAGAAATTTTATGGAACTTTCGATATTCAGCGCGACCACGT
ATCGCTACTTAAACGTGCCATGAACCGTCTAAGCGCCGAAGGCACGCTATACTTCTCAAATAACTATCGTGGTTTTGAGT
TAGATGAGGAAATTGATGCAATTTTCTACGCGCAAGAAATTACCAATGATACCATTGGGCCAGATTTCAAACGTAATCAG
AAAATTCATCGTGCGTGGAAAATTCAGCATCCACATATGAACTAA

Upstream 100 bases:

>100_bases
CAATTGCATAAAAAAGACCATTTCAATTTTAATCGCTAAGTTTTCATCTCTAAAATACGCTAAAATAGTCGATTGCTTTT
GCTGCTTTTTGGAAAATTGA

Downstream 100 bases:

>100_bases
TCATTATAGAGATGTCAGTTATTTCACTGTAATACTGACATCTCCCCTTGCTCCAGATGCATCTATACCGCGAAATAAAA
ACTCATTAAAAGAAGTATCT

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 734; Mature: 734

Protein sequence:

>734_residues
MNSPQRLSTYWVTCADGLETLLQQELQGLGIEQTERFAGRLIFQGSLEQAYRVCMWSRLASRVLKPIHTFELERTHDARD
VAEELYEGALSFDWSLIFAPQSTFAIRLHVEREIKVNSQFATLRVKDGVVDSFMEAVGRRPSIDTKQPEITLFVLAGKTE
HTYCLDLSGDSLHKRGYRHYMTDAPIKENLAAAILQKAKLTELKPDLILDPMCGSGTFIIESLMIFTDRAPGLVRRFGFN
GWHGHDRELWLELKAEAAERHEIALQNTLPKFYAFDADWEAVKATRQNIIAAGFEKLLDQIQIEERTLADWPSFENTYNT
AFVVTNPPYGERLGDKASNRALYLGLSALLQQYFPKQHAAVIAAQIEQADVLAFSEPETLRLMNGKLPIYIRLGQVKPVA
NSQPFLANWHADPVEIEGAQDFANRLQKNMSALKKWAVKDQVFCLRLYDADLPDFNVAVDLYGDRLHVQEYAPPKTIDPE
KAKKRFNLALAAIRAVTGLPREAIFIKTRARQEGKNQYTKQSTASKRFIVQEGQAKILVNLTDYLDTGLFLDHRQIRLRI
AQEARGKHFLNLFSYTSTASLHAALGGAASTTSVDLSNTYINWSKENFVLNGLTVDHADEQHMFFASDCFEWLKEGHEQY
DLIFIDPPTFSNSKKFYGTFDIQRDHVSLLKRAMNRLSAEGTLYFSNNYRGFELDEEIDAIFYAQEITNDTIGPDFKRNQ
KIHRAWKIQHPHMN

Sequences:

>Translated_734_residues
MNSPQRLSTYWVTCADGLETLLQQELQGLGIEQTERFAGRLIFQGSLEQAYRVCMWSRLASRVLKPIHTFELERTHDARD
VAEELYEGALSFDWSLIFAPQSTFAIRLHVEREIKVNSQFATLRVKDGVVDSFMEAVGRRPSIDTKQPEITLFVLAGKTE
HTYCLDLSGDSLHKRGYRHYMTDAPIKENLAAAILQKAKLTELKPDLILDPMCGSGTFIIESLMIFTDRAPGLVRRFGFN
GWHGHDRELWLELKAEAAERHEIALQNTLPKFYAFDADWEAVKATRQNIIAAGFEKLLDQIQIEERTLADWPSFENTYNT
AFVVTNPPYGERLGDKASNRALYLGLSALLQQYFPKQHAAVIAAQIEQADVLAFSEPETLRLMNGKLPIYIRLGQVKPVA
NSQPFLANWHADPVEIEGAQDFANRLQKNMSALKKWAVKDQVFCLRLYDADLPDFNVAVDLYGDRLHVQEYAPPKTIDPE
KAKKRFNLALAAIRAVTGLPREAIFIKTRARQEGKNQYTKQSTASKRFIVQEGQAKILVNLTDYLDTGLFLDHRQIRLRI
AQEARGKHFLNLFSYTSTASLHAALGGAASTTSVDLSNTYINWSKENFVLNGLTVDHADEQHMFFASDCFEWLKEGHEQY
DLIFIDPPTFSNSKKFYGTFDIQRDHVSLLKRAMNRLSAEGTLYFSNNYRGFELDEEIDAIFYAQEITNDTIGPDFKRNQ
KIHRAWKIQHPHMN
>Mature_734_residues
MNSPQRLSTYWVTCADGLETLLQQELQGLGIEQTERFAGRLIFQGSLEQAYRVCMWSRLASRVLKPIHTFELERTHDARD
VAEELYEGALSFDWSLIFAPQSTFAIRLHVEREIKVNSQFATLRVKDGVVDSFMEAVGRRPSIDTKQPEITLFVLAGKTE
HTYCLDLSGDSLHKRGYRHYMTDAPIKENLAAAILQKAKLTELKPDLILDPMCGSGTFIIESLMIFTDRAPGLVRRFGFN
GWHGHDRELWLELKAEAAERHEIALQNTLPKFYAFDADWEAVKATRQNIIAAGFEKLLDQIQIEERTLADWPSFENTYNT
AFVVTNPPYGERLGDKASNRALYLGLSALLQQYFPKQHAAVIAAQIEQADVLAFSEPETLRLMNGKLPIYIRLGQVKPVA
NSQPFLANWHADPVEIEGAQDFANRLQKNMSALKKWAVKDQVFCLRLYDADLPDFNVAVDLYGDRLHVQEYAPPKTIDPE
KAKKRFNLALAAIRAVTGLPREAIFIKTRARQEGKNQYTKQSTASKRFIVQEGQAKILVNLTDYLDTGLFLDHRQIRLRI
AQEARGKHFLNLFSYTSTASLHAALGGAASTTSVDLSNTYINWSKENFVLNGLTVDHADEQHMFFASDCFEWLKEGHEQY
DLIFIDPPTFSNSKKFYGTFDIQRDHVSLLKRAMNRLSAEGTLYFSNNYRGFELDEEIDAIFYAQEITNDTIGPDFKRNQ
KIHRAWKIQHPHMN

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=727, Percent_Identity=42.2283356258597, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI145693111, Length=259, Percent_Identity=27.7992277992278, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_ACIAD (Q6FCR7)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045964.1
- ProteinModelPortal:   Q6FCR7
- SMR:   Q6FCR7
- STRING:   Q6FCR7
- GeneID:   2878692
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1268
- NMPDR:   fig|62977.3.peg.393
- eggNOG:   COG0116
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- PhylomeDB:   Q6FCR7
- ProtClustDB:   PRK11783
- BioCyc:   ASP62977:ACIAD1268-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- PRINTS:   PR00507
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 83789; Mature: 83789

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00024 HEMOPEXIN ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPQRLSTYWVTCADGLETLLQQELQGLGIEQTERFAGRLIFQGSLEQAYRVCMWSRLA
CCCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCHHHHHHCEEEEECCHHHHHHHHHHHHHH
SRVLKPIHTFELERTHDARDVAEELYEGALSFDWSLIFAPQSTFAIRLHVEREIKVNSQF
HHHHHHHHHEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEEEEEEEECCEE
ATLRVKDGVVDSFMEAVGRRPSIDTKQPEITLFVLAGKTEHTYCLDLSGDSLHKRGYRHY
EEEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCHHHHCCCHHH
MTDAPIKENLAAAILQKAKLTELKPDLILDPMCGSGTFIIESLMIFTDRAPGLVRRFGFN
CCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHCCCCCHHHHHCCCC
GWHGHDRELWLELKAEAAERHEIALQNTLPKFYAFDADWEAVKATRQNIIAAGFEKLLDQ
CCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
IQIEERTLADWPSFENTYNTAFVVTNPPYGERLGDKASNRALYLGLSALLQQYFPKQHAA
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHCCCCCCCCEEEEEHHHHHHHHCCHHHHH
VIAAQIEQADVLAFSEPETLRLMNGKLPIYIRLGQVKPVANSQPFLANWHADPVEIEGAQ
HHHHHHCCCCEEEECCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEECCCCCEEECCHH
DFANRLQKNMSALKKWAVKDQVFCLRLYDADLPDFNVAVDLYGDRLHVQEYAPPKTIDPE
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEECCEEEEECCCCCCCCCHH
KAKKRFNLALAAIRAVTGLPREAIFIKTRARQEGKNQYTKQSTASKRFIVQEGQAKILVN
HHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEE
LTDYLDTGLFLDHRQIRLRIAQEARGKHFLNLFSYTSTASLHAALGGAASTTSVDLSNTY
EHHHHCCCEEECCHHHHEEEHHHHCCCHHHHHHHHCCCCHHHHHHCCCCCCCEEECCCCE
INWSKENFVLNGLTVDHADEQHMFFASDCFEWLKEGHEQYDLIFIDPPTFSNSKKFYGTF
EEECCCCEEEECEEECCCCCCCEEEHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEE
DIQRDHVSLLKRAMNRLSAEGTLYFSNNYRGFELDEEIDAIFYAQEITNDTIGPDFKRNQ
ECCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHEEHHHHCCCCCCCCCCCCC
KIHRAWKIQHPHMN
HHHHHEECCCCCCC
>Mature Secondary Structure
MNSPQRLSTYWVTCADGLETLLQQELQGLGIEQTERFAGRLIFQGSLEQAYRVCMWSRLA
CCCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCHHHHHHCEEEEECCHHHHHHHHHHHHHH
SRVLKPIHTFELERTHDARDVAEELYEGALSFDWSLIFAPQSTFAIRLHVEREIKVNSQF
HHHHHHHHHEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEEEEEEEECCEE
ATLRVKDGVVDSFMEAVGRRPSIDTKQPEITLFVLAGKTEHTYCLDLSGDSLHKRGYRHY
EEEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCHHHHCCCHHH
MTDAPIKENLAAAILQKAKLTELKPDLILDPMCGSGTFIIESLMIFTDRAPGLVRRFGFN
CCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHCCCCCHHHHHCCCC
GWHGHDRELWLELKAEAAERHEIALQNTLPKFYAFDADWEAVKATRQNIIAAGFEKLLDQ
CCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
IQIEERTLADWPSFENTYNTAFVVTNPPYGERLGDKASNRALYLGLSALLQQYFPKQHAA
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHCCCCCCCCEEEEEHHHHHHHHCCHHHHH
VIAAQIEQADVLAFSEPETLRLMNGKLPIYIRLGQVKPVANSQPFLANWHADPVEIEGAQ
HHHHHHCCCCEEEECCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEECCCCCEEECCHH
DFANRLQKNMSALKKWAVKDQVFCLRLYDADLPDFNVAVDLYGDRLHVQEYAPPKTIDPE
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEECCEEEEECCCCCCCCCHH
KAKKRFNLALAAIRAVTGLPREAIFIKTRARQEGKNQYTKQSTASKRFIVQEGQAKILVN
HHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEE
LTDYLDTGLFLDHRQIRLRIAQEARGKHFLNLFSYTSTASLHAALGGAASTTSVDLSNTY
EHHHHCCCEEECCHHHHEEEHHHHCCCHHHHHHHHCCCCHHHHHHCCCCCCCEEECCCCE
INWSKENFVLNGLTVDHADEQHMFFASDCFEWLKEGHEQYDLIFIDPPTFSNSKKFYGTF
EEECCCCEEEECEEECCCCCCCEEEHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEE
DIQRDHVSLLKRAMNRLSAEGTLYFSNNYRGFELDEEIDAIFYAQEITNDTIGPDFKRNQ
ECCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHEEHHHHCCCCCCCCCCCCC
KIHRAWKIQHPHMN
HHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA