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The map label for this gene is vfr [H]

Identifier: 50084448

GI number: 50084448

Start: 1260645

End: 1261274

Strand: Reverse

Name: vfr [H]

Synonym: ACIAD1262

Alternate gene names: 50084448

Gene position: 1261274-1260645 (Counterclockwise)

Preceding gene: 50084454

Following gene: 50084447

Centisome position: 35.05

GC content: 43.02

Gene sequence:

>630_bases
TTGAAAAAAGCGAATATTAATCGCTACCCTAAACGTACAACGATTGTCGATGCAGGCTCTGAGTCGAAATCACTTTATCT
TATTTTAAAAGGTTCAGTTTCTATTATTTTGCGCGAAGATGATGAACGCGAAATTGTTGTTGCTTACTTAAATCCAGGCG
ATTTCTTTGGCGAGATGGGTTTATTTGAGTCTAACCCACAACGTACAGCAGAAGTGAGAACCCGTGATGTCTGTGAAATT
GCTGAAATCTCTTACGACAATTTCCACGAACTGAGCAAACAGTTTCCAGACTTAAGTTACGCCGTTTTTGCTCAACTGGT
GCGCCGTTTGAAAAACACCACCCGTAAAGTCACCGATTTGGCTTTTATCGATGTTTCAGGTCGTATCGCACGTTGCTTAA
TTGACCTCGCCAGCCAGCCAGAAGCGATGATCCTGCCAAATGGCCGTCAGATTCGTATTACGCGCCAAGAGATTGGCCGT
ATTGTCGGTTGCTCACGTGAAATGGTCGGTCGTGTATTAAAAACACTCGAAGAGCAAGGCATGATTCAAACTGATGGTAA
AGCGATCCTGATTTTTGATGCTTCACTGGAAGAGCAATCATTCCATGAAGACGATGAAGATGACGAATAA

Upstream 100 bases:

>100_bases
TTATCCTCTTCAGAAACATTAAGTAATGACATCAAATTTCTCACAACTTAGTACAGATGCTCTATCACCAGGCCAGCTTC
CCGAGTCGGTCAAGGCATTA

Downstream 100 bases:

>100_bases
CTTAATCGGTATGTCGTAATCTTAAGAGAGCAAACCTATAGGTTTGCTTTTTTTATGAATAGAAGCTTTCCAAAGATACG
AAAAACTTATACGAACATAT

Product: cAMP-regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MKKANINRYPKRTTIVDAGSESKSLYLILKGSVSIILREDDEREIVVAYLNPGDFFGEMGLFESNPQRTAEVRTRDVCEI
AEISYDNFHELSKQFPDLSYAVFAQLVRRLKNTTRKVTDLAFIDVSGRIARCLIDLASQPEAMILPNGRQIRITRQEIGR
IVGCSREMVGRVLKTLEEQGMIQTDGKAILIFDASLEEQSFHEDDEDDE

Sequences:

>Translated_209_residues
MKKANINRYPKRTTIVDAGSESKSLYLILKGSVSIILREDDEREIVVAYLNPGDFFGEMGLFESNPQRTAEVRTRDVCEI
AEISYDNFHELSKQFPDLSYAVFAQLVRRLKNTTRKVTDLAFIDVSGRIARCLIDLASQPEAMILPNGRQIRITRQEIGR
IVGCSREMVGRVLKTLEEQGMIQTDGKAILIFDASLEEQSFHEDDEDDE
>Mature_209_residues
MKKANINRYPKRTTIVDAGSESKSLYLILKGSVSIILREDDEREIVVAYLNPGDFFGEMGLFESNPQRTAEVRTRDVCEI
AEISYDNFHELSKQFPDLSYAVFAQLVRRLKNTTRKVTDLAFIDVSGRIARCLIDLASQPEAMILPNGRQIRITRQEIGR
IVGCSREMVGRVLKTLEEQGMIQTDGKAILIFDASLEEQSFHEDDEDDE

Specific function: Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=192, Percent_Identity=48.9583333333333, Blast_Score=218, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 23764; Mature: 23764

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00889 CNMP_BINDING_2 ; PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKANINRYPKRTTIVDAGSESKSLYLILKGSVSIILREDDEREIVVAYLNPGDFFGEMG
CCCCCCCCCCCEEEEEECCCCCCEEEEEEECCEEEEEEECCCCEEEEEEECCCHHHHCCC
LFESNPQRTAEVRTRDVCEIAEISYDNFHELSKQFPDLSYAVFAQLVRRLKNTTRKVTDL
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHE
AFIDVSGRIARCLIDLASQPEAMILPNGRQIRITRQEIGRIVGCSREMVGRVLKTLEEQG
EEEECCCHHHHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHCC
MIQTDGKAILIFDASLEEQSFHEDDEDDE
CEEECCCEEEEEECCCCCHHCCCCCCCCC
>Mature Secondary Structure
MKKANINRYPKRTTIVDAGSESKSLYLILKGSVSIILREDDEREIVVAYLNPGDFFGEMG
CCCCCCCCCCCEEEEEECCCCCCEEEEEEECCEEEEEEECCCCEEEEEEECCCHHHHCCC
LFESNPQRTAEVRTRDVCEIAEISYDNFHELSKQFPDLSYAVFAQLVRRLKNTTRKVTDL
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHE
AFIDVSGRIARCLIDLASQPEAMILPNGRQIRITRQEIGRIVGCSREMVGRVLKTLEEQG
EEEECCCHHHHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHCC
MIQTDGKAILIFDASLEEQSFHEDDEDDE
CEEECCCEEEEEECCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002577; 10984043 [H]