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The map label for this gene is dacA [H]

Identifier: 50084374

GI number: 50084374

Start: 1181257

End: 1182567

Strand: Reverse

Name: dacA [H]

Synonym: ACIAD1184

Alternate gene names: 50084374

Gene position: 1182567-1181257 (Counterclockwise)

Preceding gene: 50084377

Following gene: 50084373

Centisome position: 32.86

GC content: 40.73

Gene sequence:

>1311_bases
GTGAGACTCTTTTTTTCTATCTTATCTTTATTCAGTCTGCTATGTGCTTCTGTTGCGCATGCCAATCTGCTTAATATCGC
GCCAGAAAGCGTCGAAGCAGAGGCATGGAGTATTGTTGATGCGCAGTCTGGACAAATTATTTCAGAACATAATAGTCACA
TTCAACGTGCACCCGCATCACTCACCAAAATGATGGTCGCCTACATTACATTAAAAGAATTAAAGGCCGGAAAACTGCAT
AAAACCGATATCCTGACCGCGACACCTGTGGTGAATATGGTGATGTGGGATGAATCGCAAATGTATTTAAAACCAGGCGA
ACAAATTTCAATAGACCAACTCTTGGCAGGCCTTATTGTTATGTCAGCCAATGATGCGGCGGTGACCTTAGCAGAACGTA
TTTCTGGTAATGTTCCAGACTTTTTGAAGCGTATGAATCAGGAAGCTCAGGCATTGGGAATGAAAGATACTTATTTTTCA
AATCCTGCTGGTGTGACCATGCCCGATCATTATTCAACATCACATGATTTGGCACTGCTTGCGCAAGCTATTGTGAAACA
AACTCCTGATTACCTGAGCTACTCCAAACTACCAAGCTTTTCTTATCAACAACGCTTTCATCGTGCGACCAATCTCGCAC
TGAAAATTGACCCGACTGCCGATGGCTTAAAAACAGGCTATACCAAAGCGGCGGGTTATAATCTGGCACTAACAGCAAAG
CGCCCGACCATGAATGTCGATATACCAGACCGCCGTATGATTGTTGTAGTATTAGGTACCAAAAATGCACATAAGCGTGC
AGAAGTGGCCAATAAACTCATGAACCTTGCTTATGCATACACGCGCGATGAAGAAGTCATTAAAAATAATCAAACGATTG
CAGAACTCCCTGTGATTAAATCGACTTTAAAAATGTTTAAGGTCGAAACACACCAACCGACTGTTGTGACCACGTCACTT
TACAATCAGCCACAAGCCATTGACCTAAACAATTTTGATAATGTTACCCAACGTTTATATTTGCCACTTGGAAATGGTCA
AAAGCAGCTTCTTGAACCACTTGAAACGACAAATACACGTATTAATGTAGAATTAAACGAACAGCAATTAACTGCACCTC
TAATGAAAGCGATTAATCTGGCGACAATACAAATCTACCAAAATAATCAGCTCATTCACAGTGTACAAATTGCAGATAAT
GTACATATTGAAGAAGCTAATTTCTTTCAACGCTTTTTTATGTGGGTCAAAGGAATCTTTAGCTTTTTTGGTCACGACCA
GCAGACAGCAAAGCTTTACCCTTTACATTAA

Upstream 100 bases:

>100_bases
GTATTTGTCTGCAAAAGTCACTAGCTATAGCCGTAAAAAAGGCATAGCATGCGTGTGTTTTTGCATTTCTCGCTAGAGCA
TGGCGACCCAAGGTGACCAC

Downstream 100 bases:

>100_bases
TGGTATCTAAACAGACTTTTAAAGGTAGCTCATGAGCTACCTTTTTTATCATCAAAAAATTTTGTCTTTGATAGATCAAT
TTCATCAAATGATCATTAAA

Product: D-ala-D-ala-carboxypeptidase, penicillin-binding protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MRLFFSILSLFSLLCASVAHANLLNIAPESVEAEAWSIVDAQSGQIISEHNSHIQRAPASLTKMMVAYITLKELKAGKLH
KTDILTATPVVNMVMWDESQMYLKPGEQISIDQLLAGLIVMSANDAAVTLAERISGNVPDFLKRMNQEAQALGMKDTYFS
NPAGVTMPDHYSTSHDLALLAQAIVKQTPDYLSYSKLPSFSYQQRFHRATNLALKIDPTADGLKTGYTKAAGYNLALTAK
RPTMNVDIPDRRMIVVVLGTKNAHKRAEVANKLMNLAYAYTRDEEVIKNNQTIAELPVIKSTLKMFKVETHQPTVVTTSL
YNQPQAIDLNNFDNVTQRLYLPLGNGQKQLLEPLETTNTRINVELNEQQLTAPLMKAINLATIQIYQNNQLIHSVQIADN
VHIEEANFFQRFFMWVKGIFSFFGHDQQTAKLYPLH

Sequences:

>Translated_436_residues
MRLFFSILSLFSLLCASVAHANLLNIAPESVEAEAWSIVDAQSGQIISEHNSHIQRAPASLTKMMVAYITLKELKAGKLH
KTDILTATPVVNMVMWDESQMYLKPGEQISIDQLLAGLIVMSANDAAVTLAERISGNVPDFLKRMNQEAQALGMKDTYFS
NPAGVTMPDHYSTSHDLALLAQAIVKQTPDYLSYSKLPSFSYQQRFHRATNLALKIDPTADGLKTGYTKAAGYNLALTAK
RPTMNVDIPDRRMIVVVLGTKNAHKRAEVANKLMNLAYAYTRDEEVIKNNQTIAELPVIKSTLKMFKVETHQPTVVTTSL
YNQPQAIDLNNFDNVTQRLYLPLGNGQKQLLEPLETTNTRINVELNEQQLTAPLMKAINLATIQIYQNNQLIHSVQIADN
VHIEEANFFQRFFMWVKGIFSFFGHDQQTAKLYPLH
>Mature_436_residues
MRLFFSILSLFSLLCASVAHANLLNIAPESVEAEAWSIVDAQSGQIISEHNSHIQRAPASLTKMMVAYITLKELKAGKLH
KTDILTATPVVNMVMWDESQMYLKPGEQISIDQLLAGLIVMSANDAAVTLAERISGNVPDFLKRMNQEAQALGMKDTYFS
NPAGVTMPDHYSTSHDLALLAQAIVKQTPDYLSYSKLPSFSYQQRFHRATNLALKIDPTADGLKTGYTKAAGYNLALTAK
RPTMNVDIPDRRMIVVVLGTKNAHKRAEVANKLMNLAYAYTRDEEVIKNNQTIAELPVIKSTLKMFKVETHQPTVVTTSL
YNQPQAIDLNNFDNVTQRLYLPLGNGQKQLLEPLETTNTRINVELNEQQLTAPLMKAINLATIQIYQNNQLIHSVQIADN
VHIEEANFFQRFFMWVKGIFSFFGHDQQTAKLYPLH

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=431, Percent_Identity=29.4663573085847, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787062, Length=434, Percent_Identity=29.7235023041475, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI87082022, Length=413, Percent_Identity=29.2978208232446, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI87082054, Length=218, Percent_Identity=31.651376146789, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 48880; Mature: 48880

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFFSILSLFSLLCASVAHANLLNIAPESVEAEAWSIVDAQSGQIISEHNSHIQRAPAS
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHH
LTKMMVAYITLKELKAGKLHKTDILTATPVVNMVMWDESQMYLKPGEQISIDQLLAGLIV
HHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEECCCCEEECCCCEECHHHHHHHHHE
MSANDAAVTLAERISGNVPDFLKRMNQEAQALGMKDTYFSNPAGVTMPDHYSTSHDLALL
ECCCCHHHHHHHHHCCCCHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
AQAIVKQTPDYLSYSKLPSFSYQQRFHRATNLALKIDPTADGLKTGYTKAAGYNLALTAK
HHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCEEEEECCCCCCHHCCCCCCCCCEEEEEEC
RPTMNVDIPDRRMIVVVLGTKNAHKRAEVANKLMNLAYAYTRDEEVIKNNQTIAELPVIK
CCEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHH
STLKMFKVETHQPTVVTTSLYNQPQAIDLNNFDNVTQRLYLPLGNGQKQLLEPLETTNTR
HHHHHHEEECCCCEEEEEECCCCCCEECCCCHHCCCCEEEEECCCCHHHHHHHHHHCCCE
INVELNEQQLTAPLMKAINLATIQIYQNNQLIHSVQIADNVHIEEANFFQRFFMWVKGIF
EEEEECHHHHHHHHHHHCCEEEEEEEECCCEEEEEEECCCCEECHHHHHHHHHHHHHHHH
SFFGHDQQTAKLYPLH
HHHCCCCCCCEEECCC
>Mature Secondary Structure
MRLFFSILSLFSLLCASVAHANLLNIAPESVEAEAWSIVDAQSGQIISEHNSHIQRAPAS
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHH
LTKMMVAYITLKELKAGKLHKTDILTATPVVNMVMWDESQMYLKPGEQISIDQLLAGLIV
HHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEECCCCEEECCCCEECHHHHHHHHHE
MSANDAAVTLAERISGNVPDFLKRMNQEAQALGMKDTYFSNPAGVTMPDHYSTSHDLALL
ECCCCHHHHHHHHHCCCCHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
AQAIVKQTPDYLSYSKLPSFSYQQRFHRATNLALKIDPTADGLKTGYTKAAGYNLALTAK
HHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCEEEEECCCCCCHHCCCCCCCCCEEEEEEC
RPTMNVDIPDRRMIVVVLGTKNAHKRAEVANKLMNLAYAYTRDEEVIKNNQTIAELPVIK
CCEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHH
STLKMFKVETHQPTVVTTSLYNQPQAIDLNNFDNVTQRLYLPLGNGQKQLLEPLETTNTR
HHHHHHEEECCCCEEEEEECCCCCCEECCCCHHCCCCEEEEECCCCHHHHHHHHHHCCCE
INVELNEQQLTAPLMKAINLATIQIYQNNQLIHSVQIADNVHIEEANFFQRFFMWVKGIF
EEEEECHHHHHHHHHHHCCEEEEEEEECCCEEEEEEECCCCEECHHHHHHHHHHHHHHHH
SFFGHDQQTAKLYPLH
HHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]