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The map label for this gene is argG [H]

Identifier: 50084343

GI number: 50084343

Start: 1142144

End: 1143484

Strand: Reverse

Name: argG [H]

Synonym: ACIAD1151

Alternate gene names: 50084343

Gene position: 1143484-1142144 (Counterclockwise)

Preceding gene: 50084344

Following gene: 50084342

Centisome position: 31.78

GC content: 42.36

Gene sequence:

>1341_bases
ATGACTGATAATGCAACCATTTTGCAGCATGTACCAGTAGGTAAAAAAGTGGGTATTGCCTTCTCTGGTGGTCTGGATAC
TTCAGCTGCTTTATTGTGGATGAAACAAAAAGGCGCAGAACCTTATGCATATACTGCAAACTTAGGTCAGCCAGATGAAG
ACGACTACGATGCGATTCCAAAAAAAGCAGAACAATACGGTGCAGTTAAAGCCCGTCTGATCGATTGTCGTTTACAGCTT
GCGCTTGAAGGTATTGCAGCAATTCAGTGTGGTGCATTCCATATCAGCACAGGCGGTGTTCCTTATTTCAATACGACACC
ACTTGGCCGTGCTGTTACAGGCACAATGCTTGTGACTGCCATGAAAGAAGATGATGTCAATATCTGGGGCGATGGTTCGA
CCTATAAAGGAAATGACATCGAACGTTTCTATCGTTATGGTCTACTCACCAATCCAAACCTAAAAATTTATAAGCCTTGG
CTTGACCAAACGTTTATTGATGAATTAGGCGGTCGTGCGGAAATGTCACAATTTCTAATCGACAACGGTTTTGACTATAA
AATGTCAAAAGAAAAAGCGTATTCAACCGATTCAAACATGCTGGGTGCAACTCACGAAGCCAAAGATCTTGAATACCTCG
ATGCAGGTATCAAGATTGTAGATCCAATCATGGGTGTTGCTTTCTGGAAAGATGAGGTTGAAATTCAACCAGAACAAGTC
AGCATTACCTTTGAAGAAGGTATGCCTGTGGCATTGAACGGTCAACGTATTGAAGATCCGGTTGAGTTCATTCTTGAAGC
AAACCGTATTGGTGGTCGTCATGGTTTAGGCATGTCAGATCAGATCGAAAACCGTATCATCGAAGCTAAATCTCGTGGTA
TTTATGAAGCTCCAGGTATGGCACTTCTGCATATTGCCTACGAACGTCTTGTGACAGGTATTCACAATGAAGATACCATC
GAACAGTACCGTATCAATGGCTTACGTTTAGGTCGTTTACTTTACCAAGGTCGCTGGTTTGATTCACAGTCACTTATGCT
TCGTGAAACTGCACAGCGCTGGGTTGCAAAAGCCATTACAGGTACAGTAACTCTAGAGTTACGTCGCGGTAATGATTACA
CCATCATGAACACTGAATCTCCAAACCTCACGTATGAAGCTGAGCGTTTAACCATGGAGAAAGGTGACTCAATGTTTAGT
CCAATGGATCGTATCGGTCAATTAACCATGCGTAATCTGGATATTACCGATACTCGTGCAAAATTAGGTATCTATACAAA
TTCTGGTCTGCTTTCTATTGGTCACGGTTCTGCTGTACCTCAGTTAGAGACTAAGAAATAA

Upstream 100 bases:

>100_bases
TCAATGCTCAGAAAAAATAAAATAAGTGCTACAATACATACCATTCCGAATTCTCAAGACCCTCGTTTCAGGTCTTTTTT
TATAAACGTTAGGATTAGTA

Downstream 100 bases:

>100_bases
TCATCTTTAAATGATCCAAATAAAAGCCGTCTATTGATGGCTTTTATTTTTTAAAGAGCTCATACGTCTCGGAATGCATC
ATGTAATGCACCTATCATGA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTDNATILQHVPVGKKVGIAFSGGLDTSAALLWMKQKGAEPYAYTANLGQPDEDDYDAIPKKAEQYGAVKARLIDCRLQL
ALEGIAAIQCGAFHISTGGVPYFNTTPLGRAVTGTMLVTAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPNLKIYKPW
LDQTFIDELGGRAEMSQFLIDNGFDYKMSKEKAYSTDSNMLGATHEAKDLEYLDAGIKIVDPIMGVAFWKDEVEIQPEQV
SITFEEGMPVALNGQRIEDPVEFILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTI
EQYRINGLRLGRLLYQGRWFDSQSLMLRETAQRWVAKAITGTVTLELRRGNDYTIMNTESPNLTYEAERLTMEKGDSMFS
PMDRIGQLTMRNLDITDTRAKLGIYTNSGLLSIGHGSAVPQLETKK

Sequences:

>Translated_446_residues
MTDNATILQHVPVGKKVGIAFSGGLDTSAALLWMKQKGAEPYAYTANLGQPDEDDYDAIPKKAEQYGAVKARLIDCRLQL
ALEGIAAIQCGAFHISTGGVPYFNTTPLGRAVTGTMLVTAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPNLKIYKPW
LDQTFIDELGGRAEMSQFLIDNGFDYKMSKEKAYSTDSNMLGATHEAKDLEYLDAGIKIVDPIMGVAFWKDEVEIQPEQV
SITFEEGMPVALNGQRIEDPVEFILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTI
EQYRINGLRLGRLLYQGRWFDSQSLMLRETAQRWVAKAITGTVTLELRRGNDYTIMNTESPNLTYEAERLTMEKGDSMFS
PMDRIGQLTMRNLDITDTRAKLGIYTNSGLLSIGHGSAVPQLETKK
>Mature_445_residues
TDNATILQHVPVGKKVGIAFSGGLDTSAALLWMKQKGAEPYAYTANLGQPDEDDYDAIPKKAEQYGAVKARLIDCRLQLA
LEGIAAIQCGAFHISTGGVPYFNTTPLGRAVTGTMLVTAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPNLKIYKPWL
DQTFIDELGGRAEMSQFLIDNGFDYKMSKEKAYSTDSNMLGATHEAKDLEYLDAGIKIVDPIMGVAFWKDEVEIQPEQVS
ITFEEGMPVALNGQRIEDPVEFILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIE
QYRINGLRLGRLLYQGRWFDSQSLMLRETAQRWVAKAITGTVTLELRRGNDYTIMNTESPNLTYEAERLTMEKGDSMFSP
MDRIGQLTMRNLDITDTRAKLGIYTNSGLLSIGHGSAVPQLETKK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=413, Percent_Identity=28.3292978208232, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI16950633, Length=413, Percent_Identity=28.3292978208232, Blast_Score=142, Evalue=5e-34,
Organism=Escherichia coli, GI1789563, Length=438, Percent_Identity=78.9954337899543, Blast_Score=725, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324514, Length=417, Percent_Identity=28.7769784172662, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21358151, Length=422, Percent_Identity=27.0142180094787, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 49707; Mature: 49576

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNATILQHVPVGKKVGIAFSGGLDTSAALLWMKQKGAEPYAYTANLGQPDEDDYDAIP
CCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEEHHCCCCCEEEECCCCCCCCCCHHHCC
KKAEQYGAVKARLIDCRLQLALEGIAAIQCGAFHISTGGVPYFNTTPLGRAVTGTMLVTA
HHHHHHCCHHEEHHHHHHHHHHHHHHEEEECEEEEECCCCCCCCCCCCCCHHCCEEEEEE
MKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPNLKIYKPWLDQTFIDELGGRAEMSQFLI
EECCCCEEEECCCCCCCCHHHHHHHHCCEECCCCEEEECCCCHHHHHHHCCHHHHHHHHH
DNGFDYKMSKEKAYSTDSNMLGATHEAKDLEYLDAGIKIVDPIMGVAFWKDEVEIQPEQV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHEECCCCEECCCEE
SITFEEGMPVALNGQRIEDPVEFILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGM
EEEECCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCH
ALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQGRWFDSQSLMLRETAQRWVAKAIT
HHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
GTVTLELRRGNDYTIMNTESPNLTYEAERLTMEKGDSMFSPMDRIGQLTMRNLDITDTRA
EEEEEEEEECCCEEEEECCCCCCEEEHHHHHHHCCCCCCCHHHHHHHHEECCCCCCCCCE
KLGIYTNSGLLSIGHGSAVPQLETKK
EEEEEECCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TDNATILQHVPVGKKVGIAFSGGLDTSAALLWMKQKGAEPYAYTANLGQPDEDDYDAIP
CCCCEEEEECCCCCEEEEEEECCCCCCCEEEEEHHCCCCCEEEECCCCCCCCCCHHHCC
KKAEQYGAVKARLIDCRLQLALEGIAAIQCGAFHISTGGVPYFNTTPLGRAVTGTMLVTA
HHHHHHCCHHEEHHHHHHHHHHHHHHEEEECEEEEECCCCCCCCCCCCCCHHCCEEEEEE
MKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPNLKIYKPWLDQTFIDELGGRAEMSQFLI
EECCCCEEEECCCCCCCCHHHHHHHHCCEECCCCEEEECCCCHHHHHHHCCHHHHHHHHH
DNGFDYKMSKEKAYSTDSNMLGATHEAKDLEYLDAGIKIVDPIMGVAFWKDEVEIQPEQV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHEECCCCEECCCEE
SITFEEGMPVALNGQRIEDPVEFILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGM
EEEECCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCH
ALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQGRWFDSQSLMLRETAQRWVAKAIT
HHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
GTVTLELRRGNDYTIMNTESPNLTYEAERLTMEKGDSMFSPMDRIGQLTMRNLDITDTRA
EEEEEEEEECCCEEEEECCCCCCEEEHHHHHHHCCCCCCCHHHHHHHHEECCCCCCCCCE
KLGIYTNSGLLSIGHGSAVPQLETKK
EEEEEECCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA