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The map label for this gene is pyrC

Identifier: 50084342

GI number: 50084342

Start: 1140913

End: 1141947

Strand: Reverse

Name: pyrC

Synonym: ACIAD1150

Alternate gene names: 50084342

Gene position: 1141947-1140913 (Counterclockwise)

Preceding gene: 50084343

Following gene: 50084341

Centisome position: 31.73

GC content: 42.51

Gene sequence:

>1035_bases
TTGGACACGATTACTCTTTTACAGCCAGATGATTGGCACGCACATTTACGTGATGGTCTTGCCCTTAAACGTACCGTTCC
AGATTTAGCTCAACAATTTGCCCGCGCAATTTGTATGCCAAATCTTGTTCCACCCGTAAAAACTGTAGATGAAGCTGAAG
CCTATCGTGAACGAATCATGGCGCATGTTCCAGAGGGTGTACATTTTGATCCGCGCATGGTGCTTTATTTTACTGACCAT
ACTTCACCAAGTGAGGTCAAGAAAATTAAAGACTCTGCCCATGTCAATGCGATCAAGCTTTATCCTGCGGGTGCAACCAC
CAACTCAGATAACGGCGTAAGTGATATTCGTAAAGTATATGCAGTGATTGAACAGCTTGAAGAACATCAGGTTCCTCTTT
TATTGCATGGTGAAGTCACCCATCATCACGTTGATATTTTTGATCGTGAAAAGCGCTTTCTAGATGAAGTTCTTTCCCCG
CTTCTCAAACAGTTTCCAAAACTTAAACTGGTACTTGAACACATCACCACCAGTGAAGCTGCACATTTTGTACTCGAACA
AGACCGTAATGTCGCTGCAACCATTACACCACAGCACTTACTCTTCAACCGCAACGATATGTTGGTGGGTGGAATTAAAC
CACATTTCTATTGTTTGCCAATTTTAAAACGCCAAACACATCAGCAAACACTGATTGAAGTGGCGACCAGCGGAAATCCT
AAATTTTTCTTGGGTACAGACAGCGCACCCCACTCAAAAAATGCCAAAGAAAATGCATGTGGTTGTGCAGGCTGCTATAG
CGCACCAACAGCGATTGAGCTCTATGCTCAAGCATTCGATCAAGTCAATAAAATTGAACGTCTAGAAGGCTTTGCAAGTC
ATTTTGGCGCAGATTTTTACGGTTTGCCACGTAACACCAATACCATTACCCTGGTCAAGGAAGATCAGATTATTCCTGAA
CAGCTTGACTATCTGGATGATGAAAAGATTATTCCACTTTATGCAGGAAAAACCATTCAATGGAGAAAAGTGTGA

Upstream 100 bases:

>100_bases
ATGATCGCAATCTGCTTTTAACGTGAATTACAGACAATTCAAGTTACCATTTTTAGCCTAATTGGATTATCATCTGTTTT
ATTTTACAGCCTGAATACCC

Downstream 100 bases:

>100_bases
CACAAGAAAATGCCACCCCTCCTGTGATTGGTCAGCGTTTCCGTGGCTTTTTACCTGTTGTTGTCGATGTCGAAACAGCA
GGTTTTAATGCCCAGACTGA

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MDTITLLQPDDWHAHLRDGLALKRTVPDLAQQFARAICMPNLVPPVKTVDEAEAYRERIMAHVPEGVHFDPRMVLYFTDH
TSPSEVKKIKDSAHVNAIKLYPAGATTNSDNGVSDIRKVYAVIEQLEEHQVPLLLHGEVTHHHVDIFDREKRFLDEVLSP
LLKQFPKLKLVLEHITTSEAAHFVLEQDRNVAATITPQHLLFNRNDMLVGGIKPHFYCLPILKRQTHQQTLIEVATSGNP
KFFLGTDSAPHSKNAKENACGCAGCYSAPTAIELYAQAFDQVNKIERLEGFASHFGADFYGLPRNTNTITLVKEDQIIPE
QLDYLDDEKIIPLYAGKTIQWRKV

Sequences:

>Translated_344_residues
MDTITLLQPDDWHAHLRDGLALKRTVPDLAQQFARAICMPNLVPPVKTVDEAEAYRERIMAHVPEGVHFDPRMVLYFTDH
TSPSEVKKIKDSAHVNAIKLYPAGATTNSDNGVSDIRKVYAVIEQLEEHQVPLLLHGEVTHHHVDIFDREKRFLDEVLSP
LLKQFPKLKLVLEHITTSEAAHFVLEQDRNVAATITPQHLLFNRNDMLVGGIKPHFYCLPILKRQTHQQTLIEVATSGNP
KFFLGTDSAPHSKNAKENACGCAGCYSAPTAIELYAQAFDQVNKIERLEGFASHFGADFYGLPRNTNTITLVKEDQIIPE
QLDYLDDEKIIPLYAGKTIQWRKV
>Mature_344_residues
MDTITLLQPDDWHAHLRDGLALKRTVPDLAQQFARAICMPNLVPPVKTVDEAEAYRERIMAHVPEGVHFDPRMVLYFTDH
TSPSEVKKIKDSAHVNAIKLYPAGATTNSDNGVSDIRKVYAVIEQLEEHQVPLLLHGEVTHHHVDIFDREKRFLDEVLSP
LLKQFPKLKLVLEHITTSEAAHFVLEQDRNVAATITPQHLLFNRNDMLVGGIKPHFYCLPILKRQTHQQTLIEVATSGNP
KFFLGTDSAPHSKNAKENACGCAGCYSAPTAIELYAQAFDQVNKIERLEGFASHFGADFYGLPRNTNTITLVKEDQIIPE
QLDYLDDEKIIPLYAGKTIQWRKV

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787301, Length=338, Percent_Identity=51.4792899408284, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6323452, Length=353, Percent_Identity=32.5779036827195, Blast_Score=182, Evalue=8e-47,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRC_ACIAD (Q6FD29)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045852.1
- HSSP:   P05020
- ProteinModelPortal:   Q6FD29
- SMR:   Q6FD29
- STRING:   Q6FD29
- GeneID:   2879564
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1150
- NMPDR:   fig|62977.3.peg.65
- eggNOG:   COG0418
- HOGENOM:   HBG628648
- OMA:   KESACGC
- PhylomeDB:   Q6FD29
- ProtClustDB:   PRK05451
- BioCyc:   ASP62977:ACIAD1150-MONOMER
- HAMAP:   MF_00219
- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195
- PIRSF:   PIRSF001237
- TIGRFAMs:   TIGR00856

Pfam domain/function: PF01979 Amidohydro_1

EC number: =3.5.2.3

Molecular weight: Translated: 38868; Mature: 38868

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00482 DIHYDROOROTASE_1; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTITLLQPDDWHAHLRDGLALKRTVPDLAQQFARAICMPNLVPPVKTVDEAEAYRERIM
CCCEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AHVPEGVHFDPRMVLYFTDHTSPSEVKKIKDSAHVNAIKLYPAGATTNSDNGVSDIRKVY
HHCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHH
AVIEQLEEHQVPLLLHGEVTHHHVDIFDREKRFLDEVLSPLLKQFPKLKLVLEHITTSEA
HHHHHHHHCCCCEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AHFVLEQDRNVAATITPQHLLFNRNDMLVGGIKPHFYCLPILKRQTHQQTLIEVATSGNP
HHHHHHCCCCEEEEECHHHHEECCCCEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCCC
KFFLGTDSAPHSKNAKENACGCAGCYSAPTAIELYAQAFDQVNKIERLEGFASHFGADFY
EEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
GLPRNTNTITLVKEDQIIPEQLDYLDDEKIIPLYAGKTIQWRKV
CCCCCCCEEEEEECCCCCHHHHCCCCCCCEEEEECCCEEEEECC
>Mature Secondary Structure
MDTITLLQPDDWHAHLRDGLALKRTVPDLAQQFARAICMPNLVPPVKTVDEAEAYRERIM
CCCEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AHVPEGVHFDPRMVLYFTDHTSPSEVKKIKDSAHVNAIKLYPAGATTNSDNGVSDIRKVY
HHCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHH
AVIEQLEEHQVPLLLHGEVTHHHVDIFDREKRFLDEVLSPLLKQFPKLKLVLEHITTSEA
HHHHHHHHCCCCEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AHFVLEQDRNVAATITPQHLLFNRNDMLVGGIKPHFYCLPILKRQTHQQTLIEVATSGNP
HHHHHHCCCCEEEEECHHHHEECCCCEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCCC
KFFLGTDSAPHSKNAKENACGCAGCYSAPTAIELYAQAFDQVNKIERLEGFASHFGADFY
EEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
GLPRNTNTITLVKEDQIIPEQLDYLDDEKIIPLYAGKTIQWRKV
CCCCCCCEEEEEECCCCCHHHHCCCCCCCEEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA