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The map label for this gene is yofA [H]

Identifier: 50084344

GI number: 50084344

Start: 1143685

End: 1144545

Strand: Reverse

Name: yofA [H]

Synonym: ACIAD1152

Alternate gene names: 50084344

Gene position: 1144545-1143685 (Counterclockwise)

Preceding gene: 50084347

Following gene: 50084343

Centisome position: 31.81

GC content: 34.73

Gene sequence:

>861_bases
ATGAATATTGATATCAGTGACATCAGATCATTTATTACAGTGGCGGAGCTCAAAAGTATTACGGCTGCGGCACAGAAGTT
GAATTATTTACAGTCCAACATGACCGCCAAAATTAAAAAAATTGAAAATCATTACAATAAACAGCTTTTTATACGAAAAC
CGAAAGGTGTGGAACTCACAGTGCCTGGTATAAATGTATATCAGCAGTTTAAAAAGATGCTCTTCACTTGGGAAGAAACT
GAAAATAAAATTCAGCAGCACGATAATGTTCTTCGCTTTGGAACCAATACCAGCTTGGGAGGTATGCGCTTTTATTCATC
GTTCAGTCAACTTTATCAAGCTTACCCTGATCTGAGCATCACCATGAAAACAGGCGCTACAGGTTATATTGAAGAAGAAA
TTCTGAATGGAAATCTTGATATTGCTTATGTACTTGGACATCCCAAACAGAAAAACCTGCAATACATTCAAAAAGCAGAG
GATCAACTGGTTCTCATTGGGAAAAACGTGATTAATCATGAGAATATACAAGATTGTCTTGATCATCAAAATATTCTTTT
TGCTTCAGAACAGTGTTGTTATGCCACGATTCTTGACAACATTTATACAGATTACAATTTAACCAAAGGTGAGTTGACGC
ATATTTATGATCATGAGGCTTTGATTCACTTTAGCCAGCTAGGTATGGGAATTTCCATGATCGCAAAATCTTTAGTCAAA
AAATTTAATATTCAATACTATCGAGAAGTCCCTGAACTCTACCGAAATATTGGTTTATATCTGATTTCCCGAAATAACCA
TGTATTCACTCCTATTGAAAAACAATTTATCGATTTAAATAATGCTTTAGAGAAAGTCTAA

Upstream 100 bases:

>100_bases
AACTGATTGTATAAAATAAATGACATCATGAATAATGTTATGTTTTAATAGATACATCATTTTTTTTGATGTCTATGCAT
CTTCATTTGAGAATCATGCC

Downstream 100 bases:

>100_bases
TCATCGGTTTTCTCTATTTTATCTTTGGGTTAAATAACAAAATCTGTATGTCCCCACCAAACTCAACAAGGAGATTTAAT
TTTTTTTCCACTCAAGATAT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MNIDISDIRSFITVAELKSITAAAQKLNYLQSNMTAKIKKIENHYNKQLFIRKPKGVELTVPGINVYQQFKKMLFTWEET
ENKIQQHDNVLRFGTNTSLGGMRFYSSFSQLYQAYPDLSITMKTGATGYIEEEILNGNLDIAYVLGHPKQKNLQYIQKAE
DQLVLIGKNVINHENIQDCLDHQNILFASEQCCYATILDNIYTDYNLTKGELTHIYDHEALIHFSQLGMGISMIAKSLVK
KFNIQYYREVPELYRNIGLYLISRNNHVFTPIEKQFIDLNNALEKV

Sequences:

>Translated_286_residues
MNIDISDIRSFITVAELKSITAAAQKLNYLQSNMTAKIKKIENHYNKQLFIRKPKGVELTVPGINVYQQFKKMLFTWEET
ENKIQQHDNVLRFGTNTSLGGMRFYSSFSQLYQAYPDLSITMKTGATGYIEEEILNGNLDIAYVLGHPKQKNLQYIQKAE
DQLVLIGKNVINHENIQDCLDHQNILFASEQCCYATILDNIYTDYNLTKGELTHIYDHEALIHFSQLGMGISMIAKSLVK
KFNIQYYREVPELYRNIGLYLISRNNHVFTPIEKQFIDLNNALEKV
>Mature_286_residues
MNIDISDIRSFITVAELKSITAAAQKLNYLQSNMTAKIKKIENHYNKQLFIRKPKGVELTVPGINVYQQFKKMLFTWEET
ENKIQQHDNVLRFGTNTSLGGMRFYSSFSQLYQAYPDLSITMKTGATGYIEEEILNGNLDIAYVLGHPKQKNLQYIQKAE
DQLVLIGKNVINHENIQDCLDHQNILFASEQCCYATILDNIYTDYNLTKGELTHIYDHEALIHFSQLGMGISMIAKSLVK
KFNIQYYREVPELYRNIGLYLISRNNHVFTPIEKQFIDLNNALEKV

Specific function: Regulates expression of the cell division protein ftsW, and is essential for cell viability during stationary phase [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=290, Percent_Identity=20, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787601, Length=191, Percent_Identity=26.1780104712042, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33077; Mature: 33077

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDISDIRSFITVAELKSITAAAQKLNYLQSNMTAKIKKIENHYNKQLFIRKPKGVELT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEE
VPGINVYQQFKKMLFTWEETENKIQQHDNVLRFGTNTSLGGMRFYSSFSQLYQAYPDLSI
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEE
TMKTGATGYIEEEILNGNLDIAYVLGHPKQKNLQYIQKAEDQLVLIGKNVINHENIQDCL
EEECCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCCCEEEEECCCCCCCHHHHHH
DHQNILFASEQCCYATILDNIYTDYNLTKGELTHIYDHEALIHFSQLGMGISMIAKSLVK
CCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHCCCHHHHHHHHHH
KFNIQYYREVPELYRNIGLYLISRNNHVFTPIEKQFIDLNNALEKV
HHCHHHHHHHHHHHHCCCEEEEECCCEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIDISDIRSFITVAELKSITAAAQKLNYLQSNMTAKIKKIENHYNKQLFIRKPKGVELT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEE
VPGINVYQQFKKMLFTWEETENKIQQHDNVLRFGTNTSLGGMRFYSSFSQLYQAYPDLSI
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEE
TMKTGATGYIEEEILNGNLDIAYVLGHPKQKNLQYIQKAEDQLVLIGKNVINHENIQDCL
EEECCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCCCEEEEECCCCCCCHHHHHH
DHQNILFASEQCCYATILDNIYTDYNLTKGELTHIYDHEALIHFSQLGMGISMIAKSLVK
CCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHCCCHHHHHHHHHH
KFNIQYYREVPELYRNIGLYLISRNNHVFTPIEKQFIDLNNALEKV
HHCHHHHHHHHHHHHCCCEEEEECCCEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA