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The map label for this gene is pilD [H]

Identifier: 50083622

GI number: 50083622

Start: 352513

End: 353373

Strand: Reverse

Name: pilD [H]

Synonym: ACIAD0360

Alternate gene names: 50083622

Gene position: 353373-352513 (Counterclockwise)

Preceding gene: 50083623

Following gene: 50083621

Centisome position: 9.82

GC content: 41.93

Gene sequence:

>861_bases
ATGTCTGACTTCATTCGTTATTTTTCTGAAAATTTAACTGCTTTATATTGTCTTGTAGGTATTTTAAGTCTATGTATAGG
AAGCTTTCTCAATGTTGTGATTTTTCGCACACCCAAAATGATGGAACAAGAATGGAAACAAGAATGTCAGATGATATTAC
ATCCAGATCAGCCACTCATCGAAAGTGAAAAGTTAACGCTCAGTTACCCTGCTTCAACTTGTCCACACTGTCATACTCCT
ATCCGCTGGTATCAAAATATTCCATTAATCAGTTGGTTATTTCTACGTGGTAAATGTCAGTCATGCAAAGCTTCCATCAG
TAGTCGTTATCCGCTGGTTGAGTTACTGACCATGCTTTGTTCGCTGCTGGTGGTTGTGGTATTTGGTGCGACACTACACA
TGCTTTTCGGTTTAGTGCTGACCTGGATATTGATTGCGCTGACCTTTATTGACTTTGATACCCAGCTTCTACCTGATCGT
TTTACCCTGACACTTGCAGGATTAGGGTTAGCTGTAAATAGCTTCACAATCTATGTTTCCCCCACTCAAGCGATTTGGGG
TTATATTGTGGGCTTCCTTTGCCTCTGGATTGTGTATTACATTTTTAAGCTGATTACCAAAAAAGAAGGCATGGGCTATG
GCGATTTTAAATTACTCGCGGCTCTAGGAGCATGGATGGGACCCATGATGCTGCCATTGATCGTGCTCTTGTCATCCTGC
GTCGGCGCCATTATTGGTATCATTTTGCTACGTAAGCGTCAGGAAAATCAGCCTTTTGCATTTGGTCCCTATATCGCGAT
TGCAGGTTGGATTGCATTTTTGTGGGGCGATCAGATTATGAAAGTCTACCTGGGAGGCTAA

Upstream 100 bases:

>100_bases
CCATGATGGAACCGCTGATTATGGCGATTTTGGGCATTTTAGTCGGTGGACTGGTTATTGCAATGTATCTTCCAATTTTC
CAAATGGGCTCTGTGGTTTA

Downstream 100 bases:

>100_bases
GGATCATGTTTATTGTGGGCCTGACCGGTGGGATTGGTAGCGGTAAATCTGCTGCAAGCCAATGGTTTGAAAAGCAAGGC
ATACAGGTAGTAGATGCCGA

Product: type 4 prepilin-like proteins leader peptide processing enzyme

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSDFIRYFSENLTALYCLVGILSLCIGSFLNVVIFRTPKMMEQEWKQECQMILHPDQPLIESEKLTLSYPASTCPHCHTP
IRWYQNIPLISWLFLRGKCQSCKASISSRYPLVELLTMLCSLLVVVVFGATLHMLFGLVLTWILIALTFIDFDTQLLPDR
FTLTLAGLGLAVNSFTIYVSPTQAIWGYIVGFLCLWIVYYIFKLITKKEGMGYGDFKLLAALGAWMGPMMLPLIVLLSSC
VGAIIGIILLRKRQENQPFAFGPYIAIAGWIAFLWGDQIMKVYLGG

Sequences:

>Translated_286_residues
MSDFIRYFSENLTALYCLVGILSLCIGSFLNVVIFRTPKMMEQEWKQECQMILHPDQPLIESEKLTLSYPASTCPHCHTP
IRWYQNIPLISWLFLRGKCQSCKASISSRYPLVELLTMLCSLLVVVVFGATLHMLFGLVLTWILIALTFIDFDTQLLPDR
FTLTLAGLGLAVNSFTIYVSPTQAIWGYIVGFLCLWIVYYIFKLITKKEGMGYGDFKLLAALGAWMGPMMLPLIVLLSSC
VGAIIGIILLRKRQENQPFAFGPYIAIAGWIAFLWGDQIMKVYLGG
>Mature_285_residues
SDFIRYFSENLTALYCLVGILSLCIGSFLNVVIFRTPKMMEQEWKQECQMILHPDQPLIESEKLTLSYPASTCPHCHTPI
RWYQNIPLISWLFLRGKCQSCKASISSRYPLVELLTMLCSLLVVVVFGATLHMLFGLVLTWILIALTFIDFDTQLLPDRF
TLTLAGLGLAVNSFTIYVSPTQAIWGYIVGFLCLWIVYYIFKLITKKEGMGYGDFKLLAALGAWMGPMMLPLIVLLSSCV
GAIIGIILLRKRQENQPFAFGPYIAIAGWIAFLWGDQIMKVYLGG

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tapA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=263, Percent_Identity=39.5437262357414, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1789732, Length=126, Percent_Identity=44.4444444444444, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 32323; Mature: 32192

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFIRYFSENLTALYCLVGILSLCIGSFLNVVIFRTPKMMEQEWKQECQMILHPDQPLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEECCCCCCC
ESEKLTLSYPASTCPHCHTPIRWYQNIPLISWLFLRGKCQSCKASISSRYPLVELLTMLC
CCCCEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHH
SLLVVVVFGATLHMLFGLVLTWILIALTFIDFDTQLLPDRFTLTLAGLGLAVNSFTIYVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHEECEEEEEC
PTQAIWGYIVGFLCLWIVYYIFKLITKKEGMGYGDFKLLAALGAWMGPMMLPLIVLLSSC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VGAIIGIILLRKRQENQPFAFGPYIAIAGWIAFLWGDQIMKVYLGG
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDFIRYFSENLTALYCLVGILSLCIGSFLNVVIFRTPKMMEQEWKQECQMILHPDQPLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEECCCCCCC
ESEKLTLSYPASTCPHCHTPIRWYQNIPLISWLFLRGKCQSCKASISSRYPLVELLTMLC
CCCCEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHH
SLLVVVVFGATLHMLFGLVLTWILIALTFIDFDTQLLPDRFTLTLAGLGLAVNSFTIYVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHEECEEEEEC
PTQAIWGYIVGFLCLWIVYYIFKLITKKEGMGYGDFKLLAALGAWMGPMMLPLIVLLSSC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VGAIIGIILLRKRQENQPFAFGPYIAIAGWIAFLWGDQIMKVYLGG
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA