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The map label for this gene is cysD

Identifier: 49480445

GI number: 49480445

Start: 5147209

End: 5148507

Strand: Reverse

Name: cysD

Synonym: BT9727_5087

Alternate gene names: 49480445

Gene position: 5148507-5147209 (Counterclockwise)

Preceding gene: 49481299

Following gene: 49480444

Centisome position: 98.3

GC content: 39.72

Gene sequence:

>1299_bases
ATGGGAGAATCATGGGGGAAAGGAACGATTTGTGTGCAAGGTGGCTATACGCCAAAGAATGGAGAACCGCGTGTTTTACC
GCTTTATCAAAGCACGACGTATAAATATGATACTTCGGATGATTTAGCAGCATTATTTAATTTAGAGGCAGAAGGATATA
TTTATACGCGTATTGGTAATCCTACTTTAGCTGCATTTGAGCAAAAGCTGGCGGAATTAGAGGGCGGTGTAGGGGCTGTT
GCAACGGCTTCTGGGCAGGCTGCTATTATGCTCGCCGTTTTAAATATTTGTAGTAGCGGAGACCACTTGCTTTGTTCTTC
CACAGTTTATGGCGGGACGTTTAATTTATTTGGGGTGAGTTTGCGTAAGCTTGGTATTGATGTTACGTTCTTTAATCCAA
ATTTAACAGCTGATGAAATTGTGGCCCTTGCTAATGATAAGACGAAACTTGTTTATGCAGAATCGCTAGGAAACCCAGCG
ATGAACGTTTTAAATTTCAAAGAATTTTCAGATGCAGCGAAAGAGTTGGAAGTACCTTTTATCGTGGATAATACATTAGC
AACCCCTTATTTATGCCAAGCATTTGAACATGGAGCAAATATTATCGTTCATTCTACGACGAAATATATTGATGGTCATG
CAAGTTCTTTGGGCGGCATTGTCATTGATGGAGGAAACTTCGATTGGACAAATGGAAAATATCCTGAACTTGTTGAACCG
GATCCAAGTTATCATGGCGTAAGTTATGTTCAAAATTTTGGAGCGGCAGCTTATATTGTGAAGGCACGTGTTCAATTATT
AAGAGACTACGGAAACTGTATGAGCCCATTTAATGCGTATATTAGCAATATCGGCTTAGAAACATTGCATTTACGAATGG
AGCGTCATAGTGAAAATGCTCTCGCAGTTGCTAAATGGCTTGCTGATCATGGACGTATTGAATGGGTGAATTATCCAGGA
TTAGATAGTAATGAAAATTACTCGTTAGCGCAAAAGTATTTGAAAAAAGGTGCGAGTGGTGTTTTAACATTCGGCATTAA
GGGAGGATTGGAAGCAGCAAAAGAATTTATCGCAAATGTAAAACTAGCAACTCTTGTAACGCATGTAGCTGATGCACGAA
CTTGCGTTATACATCCTGCTAGTACAACGCATAGACAATTAAGTGCTGAAGATCAACGTTTAGCTGGCGTCACATCAGAT
TTAATTCGTTTATCAGTTGGCATAGAAGATGTTTTTGATATTATTGCAGATTTAGAAGCAGCGCTAGTCGGAGGCAAACA
ACATGCCGATCATAATTGA

Upstream 100 bases:

>100_bases
GATAAGAAAGAGCCTTTAGAGCGTGTTTTCAAACTGCTCCTTTCTTGTTTTCAGGAAAGGGGCAGTTTTTTATTTTGTAT
AAAAGAAAGGAGAATGAGAA

Downstream 100 bases:

>100_bases
TAAAGATTTACCAGCTCGCAAAGTATTGCAGGAGGAAAATATTTTTGTAATGACAAAAGAGCGAGCAGAAACACAAGATA
TCCGGGCATTGAAAATTGCT

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MGESWGKGTICVQGGYTPKNGEPRVLPLYQSTTYKYDTSDDLAALFNLEAEGYIYTRIGNPTLAAFEQKLAELEGGVGAV
ATASGQAAIMLAVLNICSSGDHLLCSSTVYGGTFNLFGVSLRKLGIDVTFFNPNLTADEIVALANDKTKLVYAESLGNPA
MNVLNFKEFSDAAKELEVPFIVDNTLATPYLCQAFEHGANIIVHSTTKYIDGHASSLGGIVIDGGNFDWTNGKYPELVEP
DPSYHGVSYVQNFGAAAYIVKARVQLLRDYGNCMSPFNAYISNIGLETLHLRMERHSENALAVAKWLADHGRIEWVNYPG
LDSNENYSLAQKYLKKGASGVLTFGIKGGLEAAKEFIANVKLATLVTHVADARTCVIHPASTTHRQLSAEDQRLAGVTSD
LIRLSVGIEDVFDIIADLEAALVGGKQHADHN

Sequences:

>Translated_432_residues
MGESWGKGTICVQGGYTPKNGEPRVLPLYQSTTYKYDTSDDLAALFNLEAEGYIYTRIGNPTLAAFEQKLAELEGGVGAV
ATASGQAAIMLAVLNICSSGDHLLCSSTVYGGTFNLFGVSLRKLGIDVTFFNPNLTADEIVALANDKTKLVYAESLGNPA
MNVLNFKEFSDAAKELEVPFIVDNTLATPYLCQAFEHGANIIVHSTTKYIDGHASSLGGIVIDGGNFDWTNGKYPELVEP
DPSYHGVSYVQNFGAAAYIVKARVQLLRDYGNCMSPFNAYISNIGLETLHLRMERHSENALAVAKWLADHGRIEWVNYPG
LDSNENYSLAQKYLKKGASGVLTFGIKGGLEAAKEFIANVKLATLVTHVADARTCVIHPASTTHRQLSAEDQRLAGVTSD
LIRLSVGIEDVFDIIADLEAALVGGKQHADHN
>Mature_431_residues
GESWGKGTICVQGGYTPKNGEPRVLPLYQSTTYKYDTSDDLAALFNLEAEGYIYTRIGNPTLAAFEQKLAELEGGVGAVA
TASGQAAIMLAVLNICSSGDHLLCSSTVYGGTFNLFGVSLRKLGIDVTFFNPNLTADEIVALANDKTKLVYAESLGNPAM
NVLNFKEFSDAAKELEVPFIVDNTLATPYLCQAFEHGANIIVHSTTKYIDGHASSLGGIVIDGGNFDWTNGKYPELVEPD
PSYHGVSYVQNFGAAAYIVKARVQLLRDYGNCMSPFNAYISNIGLETLHLRMERHSENALAVAKWLADHGRIEWVNYPGL
DSNENYSLAQKYLKKGASGVLTFGIKGGLEAAKEFIANVKLATLVTHVADARTCVIHPASTTHRQLSAEDQRLAGVTSDL
IRLSVGIEDVFDIIADLEAALVGGKQHADHN

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=403, Percent_Identity=30.5210918114144, Blast_Score=179, Evalue=6e-45,
Organism=Homo sapiens, GI25453487, Length=401, Percent_Identity=28.1795511221945, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI299473759, Length=403, Percent_Identity=27.2952853598015, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1790375, Length=395, Percent_Identity=31.1392405063291, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789383, Length=374, Percent_Identity=28.6096256684492, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17560056, Length=408, Percent_Identity=34.8039215686275, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17538055, Length=408, Percent_Identity=33.3333333333333, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI71980708, Length=408, Percent_Identity=31.3725490196078, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI193202265, Length=97, Percent_Identity=37.1134020618557, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323333, Length=412, Percent_Identity=42.7184466019417, Blast_Score=316, Evalue=5e-87,
Organism=Saccharomyces cerevisiae, GI6319307, Length=402, Percent_Identity=31.8407960199005, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321170, Length=328, Percent_Identity=28.6585365853659, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321254, Length=438, Percent_Identity=24.8858447488584, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321904, Length=406, Percent_Identity=23.8916256157635, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19922554, Length=422, Percent_Identity=33.4123222748815, Blast_Score=199, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46607; Mature: 46476

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGESWGKGTICVQGGYTPKNGEPRVLPLYQSTTYKYDTSDDLAALFNLEAEGYIYTRIGN
CCCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEECCCCCHHHHEECCCCCEEEEEECC
PTLAAFEQKLAELEGGVGAVATASGQAAIMLAVLNICSSGDHLLCSSTVYGGTFNLFGVS
CHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECEECCCCHHHHHHH
LRKLGIDVTFFNPNLTADEIVALANDKTKLVYAESLGNPAMNVLNFKEFSDAAKELEVPF
HHHHCEEEEEECCCCCHHHEEEEECCCEEEEEECCCCCCHHHHHCHHHHHHHHHHCCCCE
IVDNTLATPYLCQAFEHGANIIVHSTTKYIDGHASSLGGIVIDGGNFDWTNGKYPELVEP
EECCCCCCHHHHHHHHCCCCEEEECCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
DPSYHGVSYVQNFGAAAYIVKARVQLLRDYGNCMSPFNAYISNIGLETLHLRMERHSENA
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHEEEEHHHCCCCH
LAVAKWLADHGRIEWVNYPGLDSNENYSLAQKYLKKGASGVLTFGIKGGLEAAKEFIANV
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCC
KLATLVTHVADARTCVIHPASTTHRQLSAEDQRLAGVTSDLIRLSVGIEDVFDIIADLEA
HHHHHHHHHHCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ALVGGKQHADHN
HHCCCCCCCCCC
>Mature Secondary Structure 
GESWGKGTICVQGGYTPKNGEPRVLPLYQSTTYKYDTSDDLAALFNLEAEGYIYTRIGN
CCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEECCCCCHHHHEECCCCCEEEEEECC
PTLAAFEQKLAELEGGVGAVATASGQAAIMLAVLNICSSGDHLLCSSTVYGGTFNLFGVS
CHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECEECCCCHHHHHHH
LRKLGIDVTFFNPNLTADEIVALANDKTKLVYAESLGNPAMNVLNFKEFSDAAKELEVPF
HHHHCEEEEEECCCCCHHHEEEEECCCEEEEEECCCCCCHHHHHCHHHHHHHHHHCCCCE
IVDNTLATPYLCQAFEHGANIIVHSTTKYIDGHASSLGGIVIDGGNFDWTNGKYPELVEP
EECCCCCCHHHHHHHHCCCCEEEECCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
DPSYHGVSYVQNFGAAAYIVKARVQLLRDYGNCMSPFNAYISNIGLETLHLRMERHSENA
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHEEEEHHHCCCCH
LAVAKWLADHGRIEWVNYPGLDSNENYSLAQKYLKKGASGVLTFGIKGGLEAAKEFIANV
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCC
KLATLVTHVADARTCVIHPASTTHRQLSAEDQRLAGVTSDLIRLSVGIEDVFDIIADLEA
HHHHHHHHHHCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ALVGGKQHADHN
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]