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The map label for this gene is yfnB [H]

Identifier: 49481299

GI number: 49481299

Start: 5148979

End: 5149674

Strand: Reverse

Name: yfnB [H]

Synonym: BT9727_5088

Alternate gene names: 49481299

Gene position: 5149674-5148979 (Counterclockwise)

Preceding gene: 49480447

Following gene: 49480445

Centisome position: 98.32

GC content: 32.04

Gene sequence:

>696_bases
ATGAAAAAATATAAAACACTATTATTTGATGTGGATGATACATTGTTAGATTTTCAAAAGGCTGAGAGGATAGCGTTACG
TATGCTTTTTGAGGAGAAAGGAATTCCTCTAACTAGTGAGATTGAAGCGCAGTATAAAAAAGTTAATAAAGGTCTTTGGG
ATGATTTTGAAGAAGGTAAAATAAATCGTGATGAAGTAGTAAATACAAGATTTTCAGTTTTATTTAAAGAATATGGACAA
GAAGTGGATGGGATATTATTTGAAAATAATTATCGCAACTACTTAGAAGAAGGGAATCAACTCATACAAGGTGCATTTGA
ATTTATAAATCAAATTGAAAGTGAGTATGATTTATATATAGTGACAAACGGTGTTTCGAAGACGCAATATAAACGTTTAC
GTAATGCAGGGTTACATTCAATGTTTAAAGATATTTTCGTTTCTGAAGATACAGGTTATCAAAAGCCAATGAAAGAGTAT
TTTGATTATGTTTTTGAAAGAATTCCTAACTTTGCTCCTGAAGAAGGGCTCATTATTGGGGATTCGTTAAGCGCTGATAT
GAGAGGTGGATATGTAGCGGGAATTGATACTTGTTGGTTTAATCCAGAAAAGAAATTAAATGATAGCAAGATTGTGCCGA
CATATGAAGTGCAGAATTTTGAAGAGTTATATGCAATATTGAAGCAGCGTGTGTAA

Upstream 100 bases:

>100_bases
ACCTCATATTTAGTATGAGATATCTCCTATTATTTTTACAAGAAAGAATGCTACGATTTACGTAAATTTGTTTTTGTAGA
AAAGAAAGTGTGAGGGGCTT

Downstream 100 bases:

>100_bases
TAAAAATAGGCAGTAGAACTTTTGTCCCTACTGCCTATTTTTTTAATTTGTAGTTTCGTTATGTTTTGGAAGTGGAAATG
CATTTCCGATAGCAGCTGCC

Product: HAD superfamily hydrolase

Products: (R)-2-hydroxyacid; halide

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKKYKTLLFDVDDTLLDFQKAERIALRMLFEEKGIPLTSEIEAQYKKVNKGLWDDFEEGKINRDEVVNTRFSVLFKEYGQ
EVDGILFENNYRNYLEEGNQLIQGAFEFINQIESEYDLYIVTNGVSKTQYKRLRNAGLHSMFKDIFVSEDTGYQKPMKEY
FDYVFERIPNFAPEEGLIIGDSLSADMRGGYVAGIDTCWFNPEKKLNDSKIVPTYEVQNFEELYAILKQRV

Sequences:

>Translated_231_residues
MKKYKTLLFDVDDTLLDFQKAERIALRMLFEEKGIPLTSEIEAQYKKVNKGLWDDFEEGKINRDEVVNTRFSVLFKEYGQ
EVDGILFENNYRNYLEEGNQLIQGAFEFINQIESEYDLYIVTNGVSKTQYKRLRNAGLHSMFKDIFVSEDTGYQKPMKEY
FDYVFERIPNFAPEEGLIIGDSLSADMRGGYVAGIDTCWFNPEKKLNDSKIVPTYEVQNFEELYAILKQRV
>Mature_231_residues
MKKYKTLLFDVDDTLLDFQKAERIALRMLFEEKGIPLTSEIEAQYKKVNKGLWDDFEEGKINRDEVVNTRFSVLFKEYGQ
EVDGILFENNYRNYLEEGNQLIQGAFEFINQIESEYDLYIVTNGVSKTQYKRLRNAGLHSMFKDIFVSEDTGYQKPMKEY
FDYVFERIPNFAPEEGLIIGDSLSADMRGGYVAGIDTCWFNPEKKLNDSKIVPTYEVQNFEELYAILKQRV

Specific function: Unknown

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. YjjG family [H]

Homologues:

Organism=Escherichia coli, GI1790833, Length=228, Percent_Identity=32.0175438596491, Blast_Score=119, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR011951 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.8.1.2

Molecular weight: Translated: 27105; Mature: 27105

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYKTLLFDVDDTLLDFQKAERIALRMLFEEKGIPLTSEIEAQYKKVNKGLWDDFEEGK
CCCHHHHEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHCCC
INRDEVVNTRFSVLFKEYGQEVDGILFENNYRNYLEEGNQLIQGAFEFINQIESEYDLYI
CCHHHHHHHHHHHHHHHHCHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VTNGVSKTQYKRLRNAGLHSMFKDIFVSEDTGYQKPMKEYFDYVFERIPNFAPEEGLIIG
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEC
DSLSADMRGGYVAGIDTCWFNPEKKLNDSKIVPTYEVQNFEELYAILKQRV
CCCCCCCCCCEEEEEHHEECCCHHCCCCCCCCCCHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MKKYKTLLFDVDDTLLDFQKAERIALRMLFEEKGIPLTSEIEAQYKKVNKGLWDDFEEGK
CCCHHHHEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHCCC
INRDEVVNTRFSVLFKEYGQEVDGILFENNYRNYLEEGNQLIQGAFEFINQIESEYDLYI
CCHHHHHHHHHHHHHHHHCHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VTNGVSKTQYKRLRNAGLHSMFKDIFVSEDTGYQKPMKEYFDYVFERIPNFAPEEGLIIG
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEC
DSLSADMRGGYVAGIDTCWFNPEKKLNDSKIVPTYEVQNFEELYAILKQRV
CCCCCCCCCCEEEEEHHEECCCHHCCCCCCCCCCHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-haloacid; H2O

Specific reaction: (S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]