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The map label for this gene is mdh

Identifier: 49476306

GI number: 49476306

Start: 1914460

End: 1915422

Strand: Reverse

Name: mdh

Synonym: BH16570

Alternate gene names: 49476306

Gene position: 1915422-1914460 (Counterclockwise)

Preceding gene: 49476307

Following gene: 49476305

Centisome position: 99.19

GC content: 41.43

Gene sequence:

>963_bases
ATGGCACGAAAGAAAATAGCTCTTATTGGTTCGGGTATGATTGGGGGTACTTTAGCACATATAATTGGACTTAAAGAACT
TGGTGATGTCGTCTTATTTGATATTGCAGAAGGTATACCACAAGGTAAAGCTCTTGATATCGCCGAATCTTCACCAGTTG
ACGGTTTTGATGTCAGTTTAACCGGTGCTAATTCCTATGATGTGATTGAAGGTGCCGATGTTGTTATTGTAACAGCAGGT
GTTGCAAGAAAACCCGGTATGAGCCGTGATGACCTTTTGGGCATTAATTTAAAGGTAATGGAACAAGTTGGTGCGGGGAT
TAAAAAATATGCATCTTCAGCATTCGTTATTTGTATTACAAATCCTCTTGATGCAATGGTCTGGGCTTTGCAAAAATTTT
CAGGTCTTCCAACACAAAAAGTGGTTGGTATGGCTGGTATTCTCGATTCAGCACGTTTTCGTCATTTCTTATCAGAGGAA
TTTAAGATCTCTGTTAAAGATGTGACTGCATTCGTTTTAGGAGGACACGGTGATTCAATGGTGCCTTTGGTGCGTTATTC
AACAGTTGGTGGTATTTCTCTACCCGATCTTGTGAAAATGGGCTGGACGACGCAAGAAAAGATTGATCAAATTATTCAAC
GTACCCGTGATGGTGGTGCAGAAATTGTTAGTTTGTTAAAAACAGGTTCTGCTTTTTATGCACCAGCAGCTTCTGCTGTC
TCTATGGCTGAAGCCTATTTGAAAGACACCAAGCGTGTTGTTCCTGTCGCAGCTTATCTTTCAGGGCAATATGGGGTCAA
AGATACCTATGTTGGTGTTCCGGTTGTCATTGGTGCTGGGGGCGTTGAGCGCGTGATTGAAATCGATCTTGATAAAGAGG
AGAAAGCTGCTTTTGAAAAATCAGTGAGTGCAGTGCAAAAACTTTGTGAAGCTTGTATTGCTGTTGCACCAGGACTCAAA
TAA

Upstream 100 bases:

>100_bases
TGATAGTGTTGAAAAGATGGCTAAAATCAAAATAAATGTTTAGCGTACCAGCGGCACTGGATGATTATACGCTAAATTAT
CTAATGTTAGGAAAAATAAA

Downstream 100 bases:

>100_bases
CTCAAGCAAGACCCTGTTCATTAAAACAGAATATTTAAAAATATTATGACGATTCAAAGATTAATTTAAGATCCATAAAA
GAAAAGGACAAGTGAATGAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MARKKIALIGSGMIGGTLAHIIGLKELGDVVLFDIAEGIPQGKALDIAESSPVDGFDVSLTGANSYDVIEGADVVIVTAG
VARKPGMSRDDLLGINLKVMEQVGAGIKKYASSAFVICITNPLDAMVWALQKFSGLPTQKVVGMAGILDSARFRHFLSEE
FKISVKDVTAFVLGGHGDSMVPLVRYSTVGGISLPDLVKMGWTTQEKIDQIIQRTRDGGAEIVSLLKTGSAFYAPAASAV
SMAEAYLKDTKRVVPVAAYLSGQYGVKDTYVGVPVVIGAGGVERVIEIDLDKEEKAAFEKSVSAVQKLCEACIAVAPGLK

Sequences:

>Translated_320_residues
MARKKIALIGSGMIGGTLAHIIGLKELGDVVLFDIAEGIPQGKALDIAESSPVDGFDVSLTGANSYDVIEGADVVIVTAG
VARKPGMSRDDLLGINLKVMEQVGAGIKKYASSAFVICITNPLDAMVWALQKFSGLPTQKVVGMAGILDSARFRHFLSEE
FKISVKDVTAFVLGGHGDSMVPLVRYSTVGGISLPDLVKMGWTTQEKIDQIIQRTRDGGAEIVSLLKTGSAFYAPAASAV
SMAEAYLKDTKRVVPVAAYLSGQYGVKDTYVGVPVVIGAGGVERVIEIDLDKEEKAAFEKSVSAVQKLCEACIAVAPGLK
>Mature_319_residues
ARKKIALIGSGMIGGTLAHIIGLKELGDVVLFDIAEGIPQGKALDIAESSPVDGFDVSLTGANSYDVIEGADVVIVTAGV
ARKPGMSRDDLLGINLKVMEQVGAGIKKYASSAFVICITNPLDAMVWALQKFSGLPTQKVVGMAGILDSARFRHFLSEEF
KISVKDVTAFVLGGHGDSMVPLVRYSTVGGISLPDLVKMGWTTQEKIDQIIQRTRDGGAEIVSLLKTGSAFYAPAASAVS
MAEAYLKDTKRVVPVAAYLSGQYGVKDTYVGVPVVIGAGGVERVIEIDLDKEEKAAFEKSVSAVQKLCEACIAVAPGLK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=312, Percent_Identity=30.4487179487179, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI221136809, Length=312, Percent_Identity=30.4487179487179, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI9257228, Length=312, Percent_Identity=31.7307692307692, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI4504973, Length=312, Percent_Identity=31.7307692307692, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI5031857, Length=305, Percent_Identity=30.1639344262295, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI260099723, Length=305, Percent_Identity=30.1639344262295, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI15082234, Length=310, Percent_Identity=27.4193548387097, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI291575128, Length=308, Percent_Identity=30.1948051948052, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI4557032, Length=308, Percent_Identity=30.1948051948052, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI260099727, Length=221, Percent_Identity=31.2217194570136, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=32, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI207028494, Length=188, Percent_Identity=31.3829787234043, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI21735621, Length=299, Percent_Identity=27.4247491638796, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI103472011, Length=308, Percent_Identity=21.1038961038961, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1789632, Length=322, Percent_Identity=30.1242236024845, Blast_Score=88, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17535107, Length=246, Percent_Identity=28.4552845528455, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17554310, Length=321, Percent_Identity=28.3489096573209, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6322765, Length=301, Percent_Identity=27.5747508305648, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320125, Length=294, Percent_Identity=27.2108843537415, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324446, Length=277, Percent_Identity=27.7978339350181, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17136226, Length=306, Percent_Identity=28.1045751633987, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI45550422, Length=306, Percent_Identity=26.4705882352941, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24647881, Length=317, Percent_Identity=27.7602523659306, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24663595, Length=291, Percent_Identity=27.4914089347079, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24663599, Length=310, Percent_Identity=25.8064516129032, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_BARHE (Q6G1M0)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_034347.1
- ProteinModelPortal:   Q6G1M0
- SMR:   Q6G1M0
- GeneID:   2865615
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH16570
- NMPDR:   fig|283166.1.peg.1475
- HOGENOM:   HBG566126
- OMA:   SINLKVM
- PhylomeDB:   Q6G1M0
- ProtClustDB:   PRK06223
- BioCyc:   BHEN283166:BH16570-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33665; Mature: 33533

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: ACT_SITE 176-176 BINDING 34-34 BINDING 83-83 BINDING 89-89 BINDING 96-96 BINDING 121-121 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKKIALIGSGMIGGTLAHIIGLKELGDVVLFDIAEGIPQGKALDIAESSPVDGFDVSL
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEE
TGANSYDVIEGADVVIVTAGVARKPGMSRDDLLGINLKVMEQVGAGIKKYASSAFVICIT
ECCCCCCEECCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKFSGLPTQKVVGMAGILDSARFRHFLSEEFKISVKDVTAFVLGGHGDSM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHEEEEEEECCCCCC
VPLVRYSTVGGISLPDLVKMGWTTQEKIDQIIQRTRDGGAEIVSLLKTGSAFYAPAASAV
CEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHH
SMAEAYLKDTKRVVPVAAYLSGQYGVKDTYVGVPVVIGAGGVERVIEIDLDKEEKAAFEK
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCCCEEEEEECCCCHHHHHHHH
SVSAVQKLCEACIAVAPGLK
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ARKKIALIGSGMIGGTLAHIIGLKELGDVVLFDIAEGIPQGKALDIAESSPVDGFDVSL
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEE
TGANSYDVIEGADVVIVTAGVARKPGMSRDDLLGINLKVMEQVGAGIKKYASSAFVICIT
ECCCCCCEECCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKFSGLPTQKVVGMAGILDSARFRHFLSEEFKISVKDVTAFVLGGHGDSM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHEEEEEEECCCCCC
VPLVRYSTVGGISLPDLVKMGWTTQEKIDQIIQRTRDGGAEIVSLLKTGSAFYAPAASAV
CEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHH
SMAEAYLKDTKRVVPVAAYLSGQYGVKDTYVGVPVVIGAGGVERVIEIDLDKEEKAAFEK
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCCCEEEEEECCCCHHHHHHHH
SVSAVQKLCEACIAVAPGLK
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA