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The map label for this gene is gltA [H]

Identifier: 49475426

GI number: 49475426

Start: 746366

End: 747661

Strand: Reverse

Name: gltA [H]

Synonym: BH06380

Alternate gene names: 49475426

Gene position: 747661-746366 (Counterclockwise)

Preceding gene: 49475427

Following gene: 49475425

Centisome position: 38.72

GC content: 38.58

Gene sequence:

>1296_bases
ATGTCTAAGAATAAAGCGCACATTACTGTGAATGATAAAAAAATAGAACTCTCCGTGCGTAAAGGTACACTTGGTCCTGA
CGTTATTGAAATTGCTTCTCTCTACAAAGAAACAGATACTTTTACTTATGATCCTGGCTTTACCTCAACTGCTTCGTGTG
AATCGAAAATCACTTATATTGATGGTAATGAAGGAATTTTGCTTTATCGTGGTTATCCTATTGACCAATTGGCTGAAAAA
GGAGACTTTCTCGAAAGCTGCTATCTTTTGCTTTACGGTGAACTGCCAACAAAACAAGAAAAAATTGATTTTGATCGCTG
TATTATGCAGCATACGATGGTTCACGAACAGTTTGCAAGATTTTTCCATGGTTTCCGTCGCGACTCTCATCCTATGGCCG
TCATGATTGCATGTCTTGGAGCTATGTCTGCATTCTATCACGACTCTATTGATATTACAGATCCTCAACAGAGAATGATC
GCTTCTATTCGTCTCATTTCAAAGGTCCCAACTCTTGCCGCTATGGCCTATAAATATAGCATTGGACAAGCATTTGTTTA
TCCACGTAATGATCTTAGTTACGCTGCAAATTTTCTCCGTATGTGTTTTTCTGTTCCTTGTGAAGAATATAAAATTAATC
CGGTGCTGACTCGAGCTATGGATAGAATCTTTACTCTTCATGCAGATCATGAACAAAATGCTTCGACATCCACTGTACGT
CTTGCAGGTTCATCAGGTGCTAATCCATTTGCATGTATTGCAGCAGGTGTTGCATGCCTTTGGGGACCAGCTCATGGTGG
AGCTAATGAAGCATGCCTAAAAATGTTACAAGAAATAGGTTCTGTTGAAAGAATTCCTGAATTCATTGCACGTGCAAAAG
ATAAAAATGATTCTTTCCGCCTTATGGGTTTTGGTCATCGAGTCTATAAAAATTATGATCCACGCGCAAAAATCATGCAA
CAAACCTGCCATGAGGTTTTAAAAGAATTGAACATTCAAAATGATCCACTTCTTGATATTGCTATCACGCTTGAAAATAT
TGCTCTAAATGATGAATATTTTATTGAAAAAAAACTTTACCCTAATGTCGATTTCTATTCTGGCATTACATTAAAAGCTC
TAGGATTTCCAACAGAAATGTTTACTGTTCTTTTTGCATTAGCACGCAGTGTCGGCTGGGTTGCGCAATGGAAAGAAATG
ATTGAGGATCCTGCACAAAAAATTAGCCGACCACGCCAACTCTACACAGGCTACGCTGCGCGTGAATATATCCCTATAGA
CAAGCGTGTAAACTAA

Upstream 100 bases:

>100_bases
ATCTACTCTCACATAATAGAAAAAGCTTCATTTATACCCCTCCCTCTCTGATGAAGCGGAATCTTGATTTTCAAGGAGAA
GGATCTGAAAGGAATAGCTT

Downstream 100 bases:

>100_bases
AAATAAAGTAACCCCTCATTAAAGCTTCAATAAATTGGAGCTTTAATCAGAATCAATTGTCTAAAATCAAAGTGTTTTTA
CAAACCTTGATTAGAAAACT

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSKNKAHITVNDKKIELSVRKGTLGPDVIEIASLYKETDTFTYDPGFTSTASCESKITYIDGNEGILLYRGYPIDQLAEK
GDFLESCYLLLYGELPTKQEKIDFDRCIMQHTMVHEQFARFFHGFRRDSHPMAVMIACLGAMSAFYHDSIDITDPQQRMI
ASIRLISKVPTLAAMAYKYSIGQAFVYPRNDLSYAANFLRMCFSVPCEEYKINPVLTRAMDRIFTLHADHEQNASTSTVR
LAGSSGANPFACIAAGVACLWGPAHGGANEACLKMLQEIGSVERIPEFIARAKDKNDSFRLMGFGHRVYKNYDPRAKIMQ
QTCHEVLKELNIQNDPLLDIAITLENIALNDEYFIEKKLYPNVDFYSGITLKALGFPTEMFTVLFALARSVGWVAQWKEM
IEDPAQKISRPRQLYTGYAAREYIPIDKRVN

Sequences:

>Translated_431_residues
MSKNKAHITVNDKKIELSVRKGTLGPDVIEIASLYKETDTFTYDPGFTSTASCESKITYIDGNEGILLYRGYPIDQLAEK
GDFLESCYLLLYGELPTKQEKIDFDRCIMQHTMVHEQFARFFHGFRRDSHPMAVMIACLGAMSAFYHDSIDITDPQQRMI
ASIRLISKVPTLAAMAYKYSIGQAFVYPRNDLSYAANFLRMCFSVPCEEYKINPVLTRAMDRIFTLHADHEQNASTSTVR
LAGSSGANPFACIAAGVACLWGPAHGGANEACLKMLQEIGSVERIPEFIARAKDKNDSFRLMGFGHRVYKNYDPRAKIMQ
QTCHEVLKELNIQNDPLLDIAITLENIALNDEYFIEKKLYPNVDFYSGITLKALGFPTEMFTVLFALARSVGWVAQWKEM
IEDPAQKISRPRQLYTGYAAREYIPIDKRVN
>Mature_430_residues
SKNKAHITVNDKKIELSVRKGTLGPDVIEIASLYKETDTFTYDPGFTSTASCESKITYIDGNEGILLYRGYPIDQLAEKG
DFLESCYLLLYGELPTKQEKIDFDRCIMQHTMVHEQFARFFHGFRRDSHPMAVMIACLGAMSAFYHDSIDITDPQQRMIA
SIRLISKVPTLAAMAYKYSIGQAFVYPRNDLSYAANFLRMCFSVPCEEYKINPVLTRAMDRIFTLHADHEQNASTSTVRL
AGSSGANPFACIAAGVACLWGPAHGGANEACLKMLQEIGSVERIPEFIARAKDKNDSFRLMGFGHRVYKNYDPRAKIMQQ
TCHEVLKELNIQNDPLLDIAITLENIALNDEYFIEKKLYPNVDFYSGITLKALGFPTEMFTVLFALARSVGWVAQWKEMI
EDPAQKISRPRQLYTGYAAREYIPIDKRVN

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=393, Percent_Identity=27.735368956743, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1786939, Length=430, Percent_Identity=69.0697674418605, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI1786527, Length=372, Percent_Identity=31.4516129032258, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17555174, Length=405, Percent_Identity=28.1481481481481, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319850, Length=367, Percent_Identity=27.2479564032698, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=354, Percent_Identity=26.8361581920904, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6325257, Length=365, Percent_Identity=25.7534246575342, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24640126, Length=407, Percent_Identity=27.7641277641278, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24640124, Length=403, Percent_Identity=28.287841191067, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21356863, Length=404, Percent_Identity=27.4752475247525, Blast_Score=101, Evalue=8e-22,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48803; Mature: 48671

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNKAHITVNDKKIELSVRKGTLGPDVIEIASLYKETDTFTYDPGFTSTASCESKITYI
CCCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEE
DGNEGILLYRGYPIDQLAEKGDFLESCYLLLYGELPTKQEKIDFDRCIMQHTMVHEQFAR
ECCCCEEEEECCCHHHHHHCCCHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHH
FFHGFRRDSHPMAVMIACLGAMSAFYHDSIDITDPQQRMIASIRLISKVPTLAAMAYKYS
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IGQAFVYPRNDLSYAANFLRMCFSVPCEEYKINPVLTRAMDRIFTLHADHEQNASTSTVR
CCCEEEECCCCHHHHHHHHHHHHHCCHHHEECCHHHHHHHHHHEEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGVACLWGPAHGGANEACLKMLQEIGSVERIPEFIARAKDKNDSFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEE
LMGFGHRVYKNYDPRAKIMQQTCHEVLKELNIQNDPLLDIAITLENIALNDEYFIEKKLY
EEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHEEECCHHEEECCCC
PNVDFYSGITLKALGFPTEMFTVLFALARSVGWVAQWKEMIEDPAQKISRPRQLYTGYAA
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHH
REYIPIDKRVN
HEECCCCCCCC
>Mature Secondary Structure 
SKNKAHITVNDKKIELSVRKGTLGPDVIEIASLYKETDTFTYDPGFTSTASCESKITYI
CCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEE
DGNEGILLYRGYPIDQLAEKGDFLESCYLLLYGELPTKQEKIDFDRCIMQHTMVHEQFAR
ECCCCEEEEECCCHHHHHHCCCHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHH
FFHGFRRDSHPMAVMIACLGAMSAFYHDSIDITDPQQRMIASIRLISKVPTLAAMAYKYS
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IGQAFVYPRNDLSYAANFLRMCFSVPCEEYKINPVLTRAMDRIFTLHADHEQNASTSTVR
CCCEEEECCCCHHHHHHHHHHHHHCCHHHEECCHHHHHHHHHHEEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGVACLWGPAHGGANEACLKMLQEIGSVERIPEFIARAKDKNDSFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEE
LMGFGHRVYKNYDPRAKIMQQTCHEVLKELNIQNDPLLDIAITLENIALNDEYFIEKKLY
EEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHEEECCHHEEECCCC
PNVDFYSGITLKALGFPTEMFTVLFALARSVGWVAQWKEMIEDPAQKISRPRQLYTGYAA
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHH
REYIPIDKRVN
HEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8863415 [H]