Click here to switch to the map view.

The map label for this gene is gltX

Identifier: 49475427

GI number: 49475427

Start: 747916

End: 749367

Strand: Reverse

Name: gltX

Synonym: BH06390

Alternate gene names: 49475427

Gene position: 749367-747916 (Counterclockwise)

Preceding gene: 49475435

Following gene: 49475426

Centisome position: 38.81

GC content: 42.08

Gene sequence:

>1452_bases
GTGCCTGTTATTACCCGCTTTGCCCCTTCACCAACAGGATTCCTCCATATCGGAGGCGCCCGTACTGCTCTCTTTAATTG
GCTTTATGCAAAACATACTGGTGGAAAAATGCTCCTACGAATTGAAGATACAGATCGAGAGCGCTCAACAGAAGCAGCTG
TAAAAGCTATTATAGATGGTCTCCACTGGATGGGACTTAGCTATGATGGTGATCCTATCTCGCAATTTGAACGAGCAGAA
CGCCACCGCCAAGTCGCTGAACAACTGGTAAAAGATGGAAAAGCTTATTATTGCTATGCCTCTCCTGAAGAATTAGCTGA
AATACGTGAAAATGCCCGAGCAGAAGGCCGCCCCCCGCGTTATGATGGACGTTGGAGAGAGCGTGATATATCGAAAGCTC
CTAAAGATATAAAACCTGTTATTCGCATCAAAGCACCACAAGACGGCGAAACAATTGTGCATGATCGCGTTCAAGGTGAT
GTTCGCTTTCCTAATAAAGATCTGGATGACTTTATTATCTTACGTTCCGATGGTTCACCCACTTATATGCATGCCGTCGT
CGTTGATGATCATGATATGGGTGTAACACATATCATACGTGGTGATGACCATCTCACAAATGCAGCCCGCCAGACAATCA
TCTTCAATGCAATGGGATGGGATATTCCTGTTATGGCACATATTCCACTTATCCATGGTGAAAATGGCGCAAAATTATCA
AAACGGCATGGCGCACTAGGTGTCGCTGCTTATCGAAAAATGGGATACCTTCCTGCTGCTTTGCGCAATTATTTAGTCCG
TCTGGGATGGAGTCATGGTGATGACGAACTTATGTCAATCGAAGATATGATTTCCTGGTTTGATATCGATGATATTAACA
AAGGCGCAGCACGTTTTGATCTCAAAAAGCTCGACGCCATCAATGGACATTATATACGCATGAGTAATAATCAAGACCTT
TTTGATGCTGCTCTTCATATTTTAAAAGAAATTGAAGGTGGATTACAAATTATGGAAAGGCTTGATGAACAACGTCGTGC
TCAATTTCTAAAAGCTATACCAAATTTGAAAGAACGTTCAAAAACACTGTGCGAACTCATTGACAATGCCTCCTTCATTT
TTACAAAACGTCCATTACAGCTTGATGAAAAAGCACAAACGCTCCTAGACAAAAATGGCCTCGCTATTTTAAAAGTTATT
TATCTGGCTTTGAAAGCTTGCCCTTCTTGGGATGTAAAAAGCTTGGATGAAACTCTTCGATCTTATGCACAAACACAAGA
TCTCAAATTTGGATCTATTGCTCAACCACTTCGTGCAGCTCTCACAGGACGCCTAACATCTCCGGGAGTCTTTGATGTCC
TTGTTTTATTAGGACGCGATGAATCTCTTAACCGTATTAATGACCAACTTGTCACAACCGCATGCTCATTGCATACAAAT
CAATGTATTTAG

Upstream 100 bases:

>100_bases
TTTACTTGCAATACGGCTATTGCACACCCTATCACCTATGCTAACATAACCTCTCCATAAAGTTCTATAAATAACATCTT
AATTTTAAAGGAAGATATCC

Downstream 100 bases:

>100_bases
TCCAATGAGAATATACTTAATCACTCTGGGGATCCAGAATAGATGCCTCAACGCAAATTAAAAGTTATCATCTTTTTCAA
ATAATCGAAATACATGTTTG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MPVITRFAPSPTGFLHIGGARTALFNWLYAKHTGGKMLLRIEDTDRERSTEAAVKAIIDGLHWMGLSYDGDPISQFERAE
RHRQVAEQLVKDGKAYYCYASPEELAEIRENARAEGRPPRYDGRWRERDISKAPKDIKPVIRIKAPQDGETIVHDRVQGD
VRFPNKDLDDFIILRSDGSPTYMHAVVVDDHDMGVTHIIRGDDHLTNAARQTIIFNAMGWDIPVMAHIPLIHGENGAKLS
KRHGALGVAAYRKMGYLPAALRNYLVRLGWSHGDDELMSIEDMISWFDIDDINKGAARFDLKKLDAINGHYIRMSNNQDL
FDAALHILKEIEGGLQIMERLDEQRRAQFLKAIPNLKERSKTLCELIDNASFIFTKRPLQLDEKAQTLLDKNGLAILKVI
YLALKACPSWDVKSLDETLRSYAQTQDLKFGSIAQPLRAALTGRLTSPGVFDVLVLLGRDESLNRINDQLVTTACSLHTN
QCI

Sequences:

>Translated_483_residues
MPVITRFAPSPTGFLHIGGARTALFNWLYAKHTGGKMLLRIEDTDRERSTEAAVKAIIDGLHWMGLSYDGDPISQFERAE
RHRQVAEQLVKDGKAYYCYASPEELAEIRENARAEGRPPRYDGRWRERDISKAPKDIKPVIRIKAPQDGETIVHDRVQGD
VRFPNKDLDDFIILRSDGSPTYMHAVVVDDHDMGVTHIIRGDDHLTNAARQTIIFNAMGWDIPVMAHIPLIHGENGAKLS
KRHGALGVAAYRKMGYLPAALRNYLVRLGWSHGDDELMSIEDMISWFDIDDINKGAARFDLKKLDAINGHYIRMSNNQDL
FDAALHILKEIEGGLQIMERLDEQRRAQFLKAIPNLKERSKTLCELIDNASFIFTKRPLQLDEKAQTLLDKNGLAILKVI
YLALKACPSWDVKSLDETLRSYAQTQDLKFGSIAQPLRAALTGRLTSPGVFDVLVLLGRDESLNRINDQLVTTACSLHTN
QCI
>Mature_482_residues
PVITRFAPSPTGFLHIGGARTALFNWLYAKHTGGKMLLRIEDTDRERSTEAAVKAIIDGLHWMGLSYDGDPISQFERAER
HRQVAEQLVKDGKAYYCYASPEELAEIRENARAEGRPPRYDGRWRERDISKAPKDIKPVIRIKAPQDGETIVHDRVQGDV
RFPNKDLDDFIILRSDGSPTYMHAVVVDDHDMGVTHIIRGDDHLTNAARQTIIFNAMGWDIPVMAHIPLIHGENGAKLSK
RHGALGVAAYRKMGYLPAALRNYLVRLGWSHGDDELMSIEDMISWFDIDDINKGAARFDLKKLDAINGHYIRMSNNQDLF
DAALHILKEIEGGLQIMERLDEQRRAQFLKAIPNLKERSKTLCELIDNASFIFTKRPLQLDEKAQTLLDKNGLAILKVIY
LALKACPSWDVKSLDETLRSYAQTQDLKFGSIAQPLRAALTGRLTSPGVFDVLVLLGRDESLNRINDQLVTTACSLHTNQ
CI

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=487, Percent_Identity=32.2381930184805, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI62241042, Length=256, Percent_Identity=26.953125, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1788743, Length=470, Percent_Identity=41.9148936170213, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI226510925, Length=282, Percent_Identity=35.1063829787234, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1786895, Length=332, Percent_Identity=22.289156626506, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=482, Percent_Identity=32.1576763485477, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17543332, Length=133, Percent_Identity=34.5864661654135, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324540, Length=494, Percent_Identity=33.6032388663968, Blast_Score=246, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6324742, Length=185, Percent_Identity=30.2702702702703, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321192, Length=230, Percent_Identity=29.1304347826087, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24665321, Length=504, Percent_Identity=32.1428571428571, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI17864488, Length=294, Percent_Identity=25.8503401360544, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_BARHE (Q6G3V8)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033468.1
- ProteinModelPortal:   Q6G3V8
- SMR:   Q6G3V8
- GeneID:   2864753
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH06390
- NMPDR:   fig|283166.1.peg.596
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   Q6G3V8
- ProtClustDB:   PRK01406
- BioCyc:   BHEN283166:BH06390-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 54499; Mature: 54368

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 241-241

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVITRFAPSPTGFLHIGGARTALFNWLYAKHTGGKMLLRIEDTDRERSTEAAVKAIIDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
LHWMGLSYDGDPISQFERAERHRQVAEQLVKDGKAYYCYASPEELAEIRENARAEGRPPR
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCC
YDGRWRERDISKAPKDIKPVIRIKAPQDGETIVHDRVQGDVRFPNKDLDDFIILRSDGSP
CCCCHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCC
TYMHAVVVDDHDMGVTHIIRGDDHLTNAARQTIIFNAMGWDIPVMAHIPLIHGENGAKLS
EEEEEEEEECCCCCEEEEECCCHHHHHHHHHHEEEEECCCCCCEEEEEEEEECCCCCCHH
KRHGALGVAAYRKMGYLPAALRNYLVRLGWSHGDDELMSIEDMISWFDIDDINKGAARFD
HHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHH
LKKLDAINGHYIRMSNNQDLFDAALHILKEIEGGLQIMERLDEQRRAQFLKAIPNLKERS
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KTLCELIDNASFIFTKRPLQLDEKAQTLLDKNGLAILKVIYLALKACPSWDVKSLDETLR
HHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
SYAQTQDLKFGSIAQPLRAALTGRLTSPGVFDVLVLLGRDESLNRINDQLVTTACSLHTN
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
QCI
CCC
>Mature Secondary Structure 
PVITRFAPSPTGFLHIGGARTALFNWLYAKHTGGKMLLRIEDTDRERSTEAAVKAIIDG
CCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
LHWMGLSYDGDPISQFERAERHRQVAEQLVKDGKAYYCYASPEELAEIRENARAEGRPPR
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCC
YDGRWRERDISKAPKDIKPVIRIKAPQDGETIVHDRVQGDVRFPNKDLDDFIILRSDGSP
CCCCHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCC
TYMHAVVVDDHDMGVTHIIRGDDHLTNAARQTIIFNAMGWDIPVMAHIPLIHGENGAKLS
EEEEEEEEECCCCCEEEEECCCHHHHHHHHHHEEEEECCCCCCEEEEEEEEECCCCCCHH
KRHGALGVAAYRKMGYLPAALRNYLVRLGWSHGDDELMSIEDMISWFDIDDINKGAARFD
HHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHH
LKKLDAINGHYIRMSNNQDLFDAALHILKEIEGGLQIMERLDEQRRAQFLKAIPNLKERS
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KTLCELIDNASFIFTKRPLQLDEKAQTLLDKNGLAILKVIYLALKACPSWDVKSLDETLR
HHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
SYAQTQDLKFGSIAQPLRAALTGRLTSPGVFDVLVLLGRDESLNRINDQLVTTACSLHTN
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
QCI
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA