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The map label for this gene is hisJ [H]

Identifier: 49475425

GI number: 49475425

Start: 744744

End: 745496

Strand: Reverse

Name: hisJ [H]

Synonym: BH06370

Alternate gene names: 49475425

Gene position: 745496-744744 (Counterclockwise)

Preceding gene: 49475426

Following gene: 49475424

Centisome position: 38.61

GC content: 35.46

Gene sequence:

>753_bases
ATGAAATTATTAGCTGTAGCCCTCATAACAAATTTAGTACTGTTCACCCAATTGGCCAATGCTAAAACGCTGAAAATTGC
AAGTGATGCTTCCTATCCCCCATTCAGTTACGTCAACTCAAATAACGAGCTTCAGGGTTTTGATATTGATATATCTTATG
CACTGTGCAAAAAAATGAATGTCGAGTGTACTATCGTCACCCAAGACTTTGAGGGAATGATTCCTGGCCTTCTTGCAAAA
AAATACGATGCTATCATTTCTTCTCTCGCTCCTACAGAAGAGCGCTTACAAAAAATTGACTTCACAGACGCATACTACAG
TACAGAATTGGTTGTAATCGTTCACAAAGATTCAGGAATTAAAGAAATCTCGGCAGAAGCTTTTAAAGATAAGAATCTTG
GTGTACAATCAAATACAACACAAGCTGTGTACGCAGAAGATCATTATGCAGCTGAGGGTGTGAATATCAAACTTTATCCT
ACAGCAATAGAAGTTAAACGTGATCTTTTAAGTCATAGGCTTGATATCGTCATCTCTGATAAACTGGCAGCAGTAAACTG
GCTAGAAAATGAAGAAAAAGATTGCTGTCAACTTCTTGGTAGTCTGAAAAAAACAAAACTGCCTATCGCAATTGCTATAC
GCAAAAATAATAACGATCTTAAAAATAAGTTCAATGAAGCAATAAAAGAAATCCGTGAGGACGGAACTTATGATAAAATT
ATGAAGAAATATTTTACATTCGATATTTATTAA

Upstream 100 bases:

>100_bases
TGAATCCCAATAAATTTTTTATATTGCTCTTAGAAAACCTTACAGATTATAAAATACATGATGATTTAATATTCTCTCGC
TTATCTGATAGGAGAACATA

Downstream 100 bases:

>100_bases
AAATATCAATATAAACTAAATTTGAACAAATTATTTATTTGTAAGAAAATTTATCTAAATTTTTGTGAGGAGCACAAGGA
TGGCCCAAAATTTAGCATTG

Product: ABC transporter, periplasmic amino acid-binding protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKLLAVALITNLVLFTQLANAKTLKIASDASYPPFSYVNSNNELQGFDIDISYALCKKMNVECTIVTQDFEGMIPGLLAK
KYDAIISSLAPTEERLQKIDFTDAYYSTELVVIVHKDSGIKEISAEAFKDKNLGVQSNTTQAVYAEDHYAAEGVNIKLYP
TAIEVKRDLLSHRLDIVISDKLAAVNWLENEEKDCCQLLGSLKKTKLPIAIAIRKNNNDLKNKFNEAIKEIREDGTYDKI
MKKYFTFDIY

Sequences:

>Translated_250_residues
MKLLAVALITNLVLFTQLANAKTLKIASDASYPPFSYVNSNNELQGFDIDISYALCKKMNVECTIVTQDFEGMIPGLLAK
KYDAIISSLAPTEERLQKIDFTDAYYSTELVVIVHKDSGIKEISAEAFKDKNLGVQSNTTQAVYAEDHYAAEGVNIKLYP
TAIEVKRDLLSHRLDIVISDKLAAVNWLENEEKDCCQLLGSLKKTKLPIAIAIRKNNNDLKNKFNEAIKEIREDGTYDKI
MKKYFTFDIY
>Mature_250_residues
MKLLAVALITNLVLFTQLANAKTLKIASDASYPPFSYVNSNNELQGFDIDISYALCKKMNVECTIVTQDFEGMIPGLLAK
KYDAIISSLAPTEERLQKIDFTDAYYSTELVVIVHKDSGIKEISAEAFKDKNLGVQSNTTQAVYAEDHYAAEGVNIKLYP
TAIEVKRDLLSHRLDIVISDKLAAVNWLENEEKDCCQLLGSLKKTKLPIAIAIRKNNNDLKNKFNEAIKEIREDGTYDKI
MKKYFTFDIY

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=39.3162393162393, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1788649, Length=255, Percent_Identity=37.2549019607843, Blast_Score=170, Evalue=7e-44,
Organism=Escherichia coli, GI1787085, Length=253, Percent_Identity=36.7588932806324, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1787088, Length=248, Percent_Identity=31.4516129032258, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788228, Length=257, Percent_Identity=27.2373540856031, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787031, Length=244, Percent_Identity=28.2786885245902, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28180; Mature: 28180

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLAVALITNLVLFTQLANAKTLKIASDASYPPFSYVNSNNELQGFDIDISYALCKKMN
CHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCEEEEEEEEHHHHHHCCC
VECTIVTQDFEGMIPGLLAKKYDAIISSLAPTEERLQKIDFTDAYYSTELVVIVHKDSGI
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEECEEEEEEEECCCCC
KEISAEAFKDKNLGVQSNTTQAVYAEDHYAAEGVNIKLYPTAIEVKRDLLSHRLDIVISD
HHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHCEEECC
KLAAVNWLENEEKDCCQLLGSLKKTKLPIAIAIRKNNNDLKNKFNEAIKEIREDGTYDKI
CHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHH
MKKYFTFDIY
HHHHHHCCCC
>Mature Secondary Structure
MKLLAVALITNLVLFTQLANAKTLKIASDASYPPFSYVNSNNELQGFDIDISYALCKKMN
CHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCEEEEEEEEHHHHHHCCC
VECTIVTQDFEGMIPGLLAKKYDAIISSLAPTEERLQKIDFTDAYYSTELVVIVHKDSGI
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEECEEEEEEEECCCCC
KEISAEAFKDKNLGVQSNTTQAVYAEDHYAAEGVNIKLYPTAIEVKRDLLSHRLDIVISD
HHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHCEEECC
KLAAVNWLENEEKDCCQLLGSLKKTKLPIAIAIRKNNNDLKNKFNEAIKEIREDGTYDKI
CHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHH
MKKYFTFDIY
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]