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The map label for this gene is mrcA1 [H]

Identifier: 49475264

GI number: 49475264

Start: 540890

End: 543040

Strand: Reverse

Name: mrcA1 [H]

Synonym: BH04690

Alternate gene names: 49475264

Gene position: 543040-540890 (Counterclockwise)

Preceding gene: 49475278

Following gene: 49475263

Centisome position: 28.12

GC content: 40.03

Gene sequence:

>2151_bases
ATGTTTGACTTTTTTAAAAAAAAGAAAGCACAACAGAATAGAACCTTTCATAGTCCTGCATTCATTGAACTAGATGCACT
TTTCGATACAGCACTTTATCGCCTCCGCTCTACTAACAGTCTCTTCTGGAAAAAGACAAAAATTATCTCACAGTATTTTC
ATATCCGCGGATGGAAACGATTGATAATCGAAATTCTTGATGAAACACTCACGTTAGGATTGATTGGTTTTACTCTTTTT
ACTATCGTTGGCGTTTCCGTTTTTGAGTTAATAAAAAAAGATTGGTATTTGCCTAAAAATTTTTCCATTCTTTTTCTAGA
TCGTTATGGAAATCCCATCGGCCACCGTGGTGCATTACCAACTGCATTCGTCCCCGTTGAAGAAATGCCCGATTATGTTA
TTAAAGCGGTTCTTGCGACAGAAGACCGCCGTTTTTTCGATCACTGGGGTATTGATCTGCAAGGACTGACTCGAGCAATT
TCCCAAAATATGCAAGCTAAGAGCGTAGTTCAAGGTGGCTCAACCCTTACACAACAACTAGCTAAAAATTTGTTCTTAAC
AAATGAGAGAACTATAACACGCAAAATCAAAGAAGCTTATCTTGCTCTTTGGCTAGAAGCAAATTTCAGCAAAAAACAAA
TTTTGCAACTTTATCTTGACCGTGCCTATATGGGAGGAAACAATTTTGGTATTACAGCAGCTGCAAAATTTTATTTTGGG
AAAAACATACGCAATATATCTCTTAGCGAATCTGCTATGTTAGCAGGACTTTTCAAGGCCCCAACCAAATATGCTCCTCA
TCGTCACCTTTTTGCCGCCCAAACACGTGCCAATGTGGTATTAGCTAATCTTGTTAATAGCGGTTTTATGACTGAGAGCC
AAATTATCAACGCCCATCGTCATCCTGCCAGGGCACTTTCAAAAATAAAAAATACTCAACCTGATTATTTTCTTGATTGG
GTATTCGACGAAGTCAAAAAAATGGCGGACCAACTTCCAAGTCATACTTTAATCATTCAAACCACTCTTGATCCAAATAT
TCAAAAAGCAGCAGAAGAATCAATCGCATATCATTTACAGCAATATGGTCAACAATATCGTGTCACACAAGCTGCCACCG
TCATACTCGACAATAACGGCGCTGTATGTGCAATTGTCGGAGGAGTGGATTATAACAAAAGCCAATTTAACAGAGCAACA
CAAGGCGGCCGACAACCTGGTTCTTCATTCAAACCTTATGTGTATGCCGCCGCAATGGAGCATGGTCTGTCTCCCTCAAC
AATCGTCCTAGATGCTCCAATCAATTGGGGAGGCTGGTCACCGAAAAATAATTCAGGTCGTTATCTCGGTAAAATCGATT
TAGCAACTGCTTTAGCTTTTTCCATTAATACGGTACCTGTCTATCTTACCTATCAATATCTTAACCGCAATACAAAACCT
ATTATAGATTTGATCAAAAATATGGGGATTCATGCGCATATTTTATCACACAAAACTATGGTTTTAGGCACATCCAATAT
GACCCCTATGGATCAAGCAACCGGTTTTAATGTCTTTGCTAATGGAGGAATGGCTGGGAATCGCCATGGTTTTACGCAAA
TCAGGACAACAAATGGACATGTGCTGTGGGACTTTGAGTATAACGGGAATAAACTACATCGAGCCCTCAGTAGCCAATCA
GCAGCTTATATGAATCAAATGATGGTTGGTGTCACAACACGAGGATCCGGTAAACGTGCCGCCCTCCCCATGACCCTTGT
TGCCGGAAAAACTGGAACATCACAATCCTATCGTGATGCATGGTTTGTTGGCTTTACTGGTAATTATACTGGAGCTGTAT
GGATGGGCAATGATGATTTTTCACCGATGAATCGAGCTTTTGGTGGTGGTATTCCTGCCATGATATGGCACACTATCATG
CTCTCTGCACACAAAAATATCATGCTCAAACAGCTCTATGGTGTCAAAGATTCTCTTCTCCCTTATCAACCACCAACACG
TCCTTACAATAATGATTCTGAACCTGCACTTCCAATACCCTATACGCTTTCCCCTGAAACATTGAATATTGTACGTCTGA
TTAACAACGATCTGAAAAAGCTATCTGTAATCTCTTTAAAAAGGGAAACTATCGGAGCTTCAAAACTTTGA

Upstream 100 bases:

>100_bases
TTTAAAGTAAGCTCTTTCTTTGTCAAAACTATAGCAAAAAAGTATTTTTACTCCATATAATGATTTGTTTGGTTACATCA
CTTTGTCATAGGCTCTTTCA

Downstream 100 bases:

>100_bases
ATTCGAGATATTTTCTATCATGTTTGTTAAGATCATTATTCCCTTTTCCATTTTTGTTTCCTCCATTTTATGTGGAACAC
TGAGTGTCGATTATATCCTC

Product: penicillin-binding protein mrcA

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 716; Mature: 716

Protein sequence:

>716_residues
MFDFFKKKKAQQNRTFHSPAFIELDALFDTALYRLRSTNSLFWKKTKIISQYFHIRGWKRLIIEILDETLTLGLIGFTLF
TIVGVSVFELIKKDWYLPKNFSILFLDRYGNPIGHRGALPTAFVPVEEMPDYVIKAVLATEDRRFFDHWGIDLQGLTRAI
SQNMQAKSVVQGGSTLTQQLAKNLFLTNERTITRKIKEAYLALWLEANFSKKQILQLYLDRAYMGGNNFGITAAAKFYFG
KNIRNISLSESAMLAGLFKAPTKYAPHRHLFAAQTRANVVLANLVNSGFMTESQIINAHRHPARALSKIKNTQPDYFLDW
VFDEVKKMADQLPSHTLIIQTTLDPNIQKAAEESIAYHLQQYGQQYRVTQAATVILDNNGAVCAIVGGVDYNKSQFNRAT
QGGRQPGSSFKPYVYAAAMEHGLSPSTIVLDAPINWGGWSPKNNSGRYLGKIDLATALAFSINTVPVYLTYQYLNRNTKP
IIDLIKNMGIHAHILSHKTMVLGTSNMTPMDQATGFNVFANGGMAGNRHGFTQIRTTNGHVLWDFEYNGNKLHRALSSQS
AAYMNQMMVGVTTRGSGKRAALPMTLVAGKTGTSQSYRDAWFVGFTGNYTGAVWMGNDDFSPMNRAFGGGIPAMIWHTIM
LSAHKNIMLKQLYGVKDSLLPYQPPTRPYNNDSEPALPIPYTLSPETLNIVRLINNDLKKLSVISLKRETIGASKL

Sequences:

>Translated_716_residues
MFDFFKKKKAQQNRTFHSPAFIELDALFDTALYRLRSTNSLFWKKTKIISQYFHIRGWKRLIIEILDETLTLGLIGFTLF
TIVGVSVFELIKKDWYLPKNFSILFLDRYGNPIGHRGALPTAFVPVEEMPDYVIKAVLATEDRRFFDHWGIDLQGLTRAI
SQNMQAKSVVQGGSTLTQQLAKNLFLTNERTITRKIKEAYLALWLEANFSKKQILQLYLDRAYMGGNNFGITAAAKFYFG
KNIRNISLSESAMLAGLFKAPTKYAPHRHLFAAQTRANVVLANLVNSGFMTESQIINAHRHPARALSKIKNTQPDYFLDW
VFDEVKKMADQLPSHTLIIQTTLDPNIQKAAEESIAYHLQQYGQQYRVTQAATVILDNNGAVCAIVGGVDYNKSQFNRAT
QGGRQPGSSFKPYVYAAAMEHGLSPSTIVLDAPINWGGWSPKNNSGRYLGKIDLATALAFSINTVPVYLTYQYLNRNTKP
IIDLIKNMGIHAHILSHKTMVLGTSNMTPMDQATGFNVFANGGMAGNRHGFTQIRTTNGHVLWDFEYNGNKLHRALSSQS
AAYMNQMMVGVTTRGSGKRAALPMTLVAGKTGTSQSYRDAWFVGFTGNYTGAVWMGNDDFSPMNRAFGGGIPAMIWHTIM
LSAHKNIMLKQLYGVKDSLLPYQPPTRPYNNDSEPALPIPYTLSPETLNIVRLINNDLKKLSVISLKRETIGASKL
>Mature_716_residues
MFDFFKKKKAQQNRTFHSPAFIELDALFDTALYRLRSTNSLFWKKTKIISQYFHIRGWKRLIIEILDETLTLGLIGFTLF
TIVGVSVFELIKKDWYLPKNFSILFLDRYGNPIGHRGALPTAFVPVEEMPDYVIKAVLATEDRRFFDHWGIDLQGLTRAI
SQNMQAKSVVQGGSTLTQQLAKNLFLTNERTITRKIKEAYLALWLEANFSKKQILQLYLDRAYMGGNNFGITAAAKFYFG
KNIRNISLSESAMLAGLFKAPTKYAPHRHLFAAQTRANVVLANLVNSGFMTESQIINAHRHPARALSKIKNTQPDYFLDW
VFDEVKKMADQLPSHTLIIQTTLDPNIQKAAEESIAYHLQQYGQQYRVTQAATVILDNNGAVCAIVGGVDYNKSQFNRAT
QGGRQPGSSFKPYVYAAAMEHGLSPSTIVLDAPINWGGWSPKNNSGRYLGKIDLATALAFSINTVPVYLTYQYLNRNTKP
IIDLIKNMGIHAHILSHKTMVLGTSNMTPMDQATGFNVFANGGMAGNRHGFTQIRTTNGHVLWDFEYNGNKLHRALSSQS
AAYMNQMMVGVTTRGSGKRAALPMTLVAGKTGTSQSYRDAWFVGFTGNYTGAVWMGNDDFSPMNRAFGGGIPAMIWHTIM
LSAHKNIMLKQLYGVKDSLLPYQPPTRPYNNDSEPALPIPYTLSPETLNIVRLINNDLKKLSVISLKRETIGASKL

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=526, Percent_Identity=33.6501901140684, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI1788867, Length=518, Percent_Identity=29.9227799227799, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI87082258, Length=261, Percent_Identity=36.0153256704981, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1789601, Length=204, Percent_Identity=32.843137254902, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 80251; Mature: 80251

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDFFKKKKAQQNRTFHSPAFIELDALFDTALYRLRSTNSLFWKKTKIISQYFHIRGWKR
CCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LIIEILDETLTLGLIGFTLFTIVGVSVFELIKKDWYLPKNFSILFLDRYGNPIGHRGALP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCC
TAFVPVEEMPDYVIKAVLATEDRRFFDHWGIDLQGLTRAISQNMQAKSVVQGGSTLTQQL
EEECCHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHCCCHHHHHHH
AKNLFLTNERTITRKIKEAYLALWLEANFSKKQILQLYLDRAYMGGNNFGITAAAKFYFG
HHHHEECCCHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHC
KNIRNISLSESAMLAGLFKAPTKYAPHRHLFAAQTRANVVLANLVNSGFMTESQIINAHR
CCCCEECCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHEEHHHCCCCCCHHHHHHHCC
HPARALSKIKNTQPDYFLDWVFDEVKKMADQLPSHTLIIQTTLDPNIQKAAEESIAYHLQ
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHH
QYGQQYRVTQAATVILDNNGAVCAIVGGVDYNKSQFNRATQGGRQPGSSFKPYVYAAAME
HHHHHEEEEEEEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEHHH
HGLSPSTIVLDAPINWGGWSPKNNSGRYLGKIDLATALAFSINTVPVYLTYQYLNRNTKP
HCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHEECCEEEEEEEEECCCCCHH
IIDLIKNMGIHAHILSHKTMVLGTSNMTPMDQATGFNVFANGGMAGNRHGFTQIRTTNGH
HHHHHHCCCCEEEEECCCEEEEECCCCCCCHHCCCCEEEECCCCCCCCCCCEEEEECCCE
VLWDFEYNGNKLHRALSSQSAAYMNQMMVGVTTRGSGKRAALPMTLVAGKTGTSQSYRDA
EEEEEEECCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEECEEEEEECCCCCCCCCCCE
WFVGFTGNYTGAVWMGNDDFSPMNRAFGGGIPAMIWHTIMLSAHKNIMLKQLYGVKDSLL
EEEEEECCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
PYQPPTRPYNNDSEPALPIPYTLSPETLNIVRLINNDLKKLSVISLKRETIGASKL
CCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHEEHHHHCCCCCC
>Mature Secondary Structure
MFDFFKKKKAQQNRTFHSPAFIELDALFDTALYRLRSTNSLFWKKTKIISQYFHIRGWKR
CCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LIIEILDETLTLGLIGFTLFTIVGVSVFELIKKDWYLPKNFSILFLDRYGNPIGHRGALP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCC
TAFVPVEEMPDYVIKAVLATEDRRFFDHWGIDLQGLTRAISQNMQAKSVVQGGSTLTQQL
EEECCHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHCCCHHHHHHH
AKNLFLTNERTITRKIKEAYLALWLEANFSKKQILQLYLDRAYMGGNNFGITAAAKFYFG
HHHHEECCCHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHC
KNIRNISLSESAMLAGLFKAPTKYAPHRHLFAAQTRANVVLANLVNSGFMTESQIINAHR
CCCCEECCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHEEHHHCCCCCCHHHHHHHCC
HPARALSKIKNTQPDYFLDWVFDEVKKMADQLPSHTLIIQTTLDPNIQKAAEESIAYHLQ
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHH
QYGQQYRVTQAATVILDNNGAVCAIVGGVDYNKSQFNRATQGGRQPGSSFKPYVYAAAME
HHHHHEEEEEEEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEHHH
HGLSPSTIVLDAPINWGGWSPKNNSGRYLGKIDLATALAFSINTVPVYLTYQYLNRNTKP
HCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHEECCEEEEEEEEECCCCCHH
IIDLIKNMGIHAHILSHKTMVLGTSNMTPMDQATGFNVFANGGMAGNRHGFTQIRTTNGH
HHHHHHCCCCEEEEECCCEEEEECCCCCCCHHCCCCEEEECCCCCCCCCCCEEEEECCCE
VLWDFEYNGNKLHRALSSQSAAYMNQMMVGVTTRGSGKRAALPMTLVAGKTGTSQSYRDA
EEEEEEECCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEECEEEEEECCCCCCCCCCCE
WFVGFTGNYTGAVWMGNDDFSPMNRAFGGGIPAMIWHTIMLSAHKNIMLKQLYGVKDSLL
EEEEEECCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
PYQPPTRPYNNDSEPALPIPYTLSPETLNIVRLINNDLKKLSVISLKRETIGASKL
CCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHEEHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]