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The map label for this gene is pleC [H]

Identifier: 49475278

GI number: 49475278

Start: 559465

End: 561786

Strand: Reverse

Name: pleC [H]

Synonym: BH04830

Alternate gene names: 49475278

Gene position: 561786-559465 (Counterclockwise)

Preceding gene: 49475279

Following gene: 49475264

Centisome position: 29.09

GC content: 39.32

Gene sequence:

>2322_bases
ATGGCTAAATTGGACGCATGTAATGCGCCAACGGAGATATATGCTGATTCAAAATCGAGCGCTCAAAATCATAAAGCGCG
AACAAAGCATATCAATCCGCTTTTTGACTCGTCCTATCAAAAACTGCTCTTTATTGAACCTTGGTTGAGGCGTTCACTTC
CATTCGTCATTGTCGTATTTCTTGTTGTGCTGGCAGCAATTCGTTTTGTCTCAATCTATGATTTGCGCCACACAATTGAT
AAAAATACGCGTTCTACGATCACTCTGTTAGCTGCTCATATTGCCAATACAATTGATCGTGACTTACTTGCCGCCACTCA
GAAAGGAAAAGCCATTGCTCTTTCTCATACTTATTTGCAAAATATCCTTAGTAATTTTCAAAACCAAGGTCTGATCAAAA
CTGGCACTGTTATCGCCATAGTCGATCAAAAGGGTAATATCTTAGCATCATCCAGTCCTGAAATCATTTTAGGAAAACCT
TTACAGGACTTTATCTCTGAGAGCATTGCTTTGCGATCTCTCAGAAAACATGCTGGAATCATGAAAGTCACAATAGGAAA
AGATGCACCTGCTCTCGCTTCATTTCATCAAACAGAAAATGGAAAATATGGTGTTTTTATCAGCGAAAAAACGCAAGAGA
TCTACAGGGAATGGCGAAAAATTTTCTCGTTAAATATAACCCTGCTCATAGGAACAGGAGGCGTCATTTTAGCTCTTCTC
TATGCTTATTACAATCAAATCGTACGAGCACGCAAAACAGATTCGATCTCAGAAAAAATTCAAAATCGCATCGATATGGC
AATGATGCGTGGACGTTGTGGACTATGGGATTGGAATATGGCAAGCGGACGTGTTTACTGGTCACGAGCAATGTATGAAA
TGCTCGGTTATATACCTCAAGATGCACTGCTTTCAATTTCACAAATTACCGCTATTATCAATCCAAACGATGCTAATTTT
TTTGATCTCGCGCAAGAGTTAATGAGTGGTAAGAAAAAGCATATTGATATTAATGTTCCCATGCGTCATGCTGATGGGCA
TTATGTATGGATGCGCATTCGTGCTGAAGTTACGGATGAAGAAGAACTTCATTTGGTTGGTATTGCTTTCGATATTAGTG
AACAGCGTCAACTCGCAGAAGAAACAGCACAAGCTGATCTTCGTATCCGTGATGCAATTGAAAATATTTCAGAATCTTTT
GTACTATGGGATTCAGAAGGACGTTTAGTCATGTCCAACAGCAAGTTTTGCGAATATGCAGCTATCCCTAAACAAGTTTT
ACAACCAGGGATCCAACGTGCAACCGTTGAAGCCATGGCACGCCCTGCAATCAATGAATATCCTCTCAAAGACGATGGTA
CTGGGAACTTAACCAGTATTCGCCAAACTGCAGATGGTTGTTGGCTCAAAATTAATGAACGACGCACCCAAGATGGAGGA
CTTGTTTGCATTGGTACAGATATTTCTGAACTGAAACAACAACAAGAAAAATTTGAAGACAGTGAACGACGTCTTTTCTC
TTTTATTCAAGAACTCAAACGTGCTCGAGGAAATGCTCAACAACGCGCAACAGAAGTTGAAAAACTTAATAAAAGTCTGA
AAGCAGAAAAAGAACGCGCTGAAAGTGCTAACAAAGCTAAATCAGAATTTTTAGCCAATATGTCCCATGAATTGCGTACG
CCTCTTAACGCCATTCTTGGCTTTTCAGATATCATGTTGCAATCTACCTTTGGTCCTCTTGGCTCACAACGCTATCAAGA
ATATATGCATGACATTTATAATTCAGGAAACCATCTTCTCACTCTTATCAATGACATCCTTGATATGTCCAAAATCGAAG
CTGGAAGATTTACCCTTGATCGTAAAAATATCGATCTTGAACCCATTATTAGTGAAGCGGTGCGGACACTTACACCACAA
GCTCAAGAAAAAAATATTTCTGTCTCAACAAATATTGCTCCAGAACTGCATGCAGAAGTTGATGGACGTGCCATGAAACA
GATCTTCCTCAATCTCATCTCCAATGCTGTGAAATTCACACCTTCTGGCGGAAGTATTAATGTTTGTGCTTTCAAGAAAA
AAAATAACCTTGTTTGTAGAATTAAAGATACAGGCGTTGGCATTCCCCAATCAGCAATCAAAAAACTCGGACAACCTTTT
GAACAAGTTGAAAATCAACTCACGAAAACCCATACCGGCTCAGGTCTTGGGTTAGCCATTTCACGCTCTTTGCTAGAATT
ACACAAAGGAAAACTTGAAATTATCTCTAAAGAAATGAAAGGTACAACTGTAACCATTACAATGCCCATTAAACAAAGTT
AA

Upstream 100 bases:

>100_bases
ACAGAGGACTCGTGTTTTGATTCATTGCACCCTATGGGGTTTATTATGAATATCGAAGCAGTTTTTAAATTACATTATTC
CATAATTAAAGGAACGAGAA

Downstream 100 bases:

>100_bases
ATTTCACCCTTTTGTTCCCGTTGTAGTAAAATCTTTTTCCGCCAAATACCCCAACGATATCCAGAAATAAAACCATCCTT
GCGCACCACACGATGGCAAG

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 773; Mature: 772

Protein sequence:

>773_residues
MAKLDACNAPTEIYADSKSSAQNHKARTKHINPLFDSSYQKLLFIEPWLRRSLPFVIVVFLVVLAAIRFVSIYDLRHTID
KNTRSTITLLAAHIANTIDRDLLAATQKGKAIALSHTYLQNILSNFQNQGLIKTGTVIAIVDQKGNILASSSPEIILGKP
LQDFISESIALRSLRKHAGIMKVTIGKDAPALASFHQTENGKYGVFISEKTQEIYREWRKIFSLNITLLIGTGGVILALL
YAYYNQIVRARKTDSISEKIQNRIDMAMMRGRCGLWDWNMASGRVYWSRAMYEMLGYIPQDALLSISQITAIINPNDANF
FDLAQELMSGKKKHIDINVPMRHADGHYVWMRIRAEVTDEEELHLVGIAFDISEQRQLAEETAQADLRIRDAIENISESF
VLWDSEGRLVMSNSKFCEYAAIPKQVLQPGIQRATVEAMARPAINEYPLKDDGTGNLTSIRQTADGCWLKINERRTQDGG
LVCIGTDISELKQQQEKFEDSERRLFSFIQELKRARGNAQQRATEVEKLNKSLKAEKERAESANKAKSEFLANMSHELRT
PLNAILGFSDIMLQSTFGPLGSQRYQEYMHDIYNSGNHLLTLINDILDMSKIEAGRFTLDRKNIDLEPIISEAVRTLTPQ
AQEKNISVSTNIAPELHAEVDGRAMKQIFLNLISNAVKFTPSGGSINVCAFKKKNNLVCRIKDTGVGIPQSAIKKLGQPF
EQVENQLTKTHTGSGLGLAISRSLLELHKGKLEIISKEMKGTTVTITMPIKQS

Sequences:

>Translated_773_residues
MAKLDACNAPTEIYADSKSSAQNHKARTKHINPLFDSSYQKLLFIEPWLRRSLPFVIVVFLVVLAAIRFVSIYDLRHTID
KNTRSTITLLAAHIANTIDRDLLAATQKGKAIALSHTYLQNILSNFQNQGLIKTGTVIAIVDQKGNILASSSPEIILGKP
LQDFISESIALRSLRKHAGIMKVTIGKDAPALASFHQTENGKYGVFISEKTQEIYREWRKIFSLNITLLIGTGGVILALL
YAYYNQIVRARKTDSISEKIQNRIDMAMMRGRCGLWDWNMASGRVYWSRAMYEMLGYIPQDALLSISQITAIINPNDANF
FDLAQELMSGKKKHIDINVPMRHADGHYVWMRIRAEVTDEEELHLVGIAFDISEQRQLAEETAQADLRIRDAIENISESF
VLWDSEGRLVMSNSKFCEYAAIPKQVLQPGIQRATVEAMARPAINEYPLKDDGTGNLTSIRQTADGCWLKINERRTQDGG
LVCIGTDISELKQQQEKFEDSERRLFSFIQELKRARGNAQQRATEVEKLNKSLKAEKERAESANKAKSEFLANMSHELRT
PLNAILGFSDIMLQSTFGPLGSQRYQEYMHDIYNSGNHLLTLINDILDMSKIEAGRFTLDRKNIDLEPIISEAVRTLTPQ
AQEKNISVSTNIAPELHAEVDGRAMKQIFLNLISNAVKFTPSGGSINVCAFKKKNNLVCRIKDTGVGIPQSAIKKLGQPF
EQVENQLTKTHTGSGLGLAISRSLLELHKGKLEIISKEMKGTTVTITMPIKQS
>Mature_772_residues
AKLDACNAPTEIYADSKSSAQNHKARTKHINPLFDSSYQKLLFIEPWLRRSLPFVIVVFLVVLAAIRFVSIYDLRHTIDK
NTRSTITLLAAHIANTIDRDLLAATQKGKAIALSHTYLQNILSNFQNQGLIKTGTVIAIVDQKGNILASSSPEIILGKPL
QDFISESIALRSLRKHAGIMKVTIGKDAPALASFHQTENGKYGVFISEKTQEIYREWRKIFSLNITLLIGTGGVILALLY
AYYNQIVRARKTDSISEKIQNRIDMAMMRGRCGLWDWNMASGRVYWSRAMYEMLGYIPQDALLSISQITAIINPNDANFF
DLAQELMSGKKKHIDINVPMRHADGHYVWMRIRAEVTDEEELHLVGIAFDISEQRQLAEETAQADLRIRDAIENISESFV
LWDSEGRLVMSNSKFCEYAAIPKQVLQPGIQRATVEAMARPAINEYPLKDDGTGNLTSIRQTADGCWLKINERRTQDGGL
VCIGTDISELKQQQEKFEDSERRLFSFIQELKRARGNAQQRATEVEKLNKSLKAEKERAESANKAKSEFLANMSHELRTP
LNAILGFSDIMLQSTFGPLGSQRYQEYMHDIYNSGNHLLTLINDILDMSKIEAGRFTLDRKNIDLEPIISEAVRTLTPQA
QEKNISVSTNIAPELHAEVDGRAMKQIFLNLISNAVKFTPSGGSINVCAFKKKNNLVCRIKDTGVGIPQSAIKKLGQPFE
QVENQLTKTHTGSGLGLAISRSLLELHKGKLEIISKEMKGTTVTITMPIKQS

Specific function: Member of the two-component regulatory system involved in the regulation of polar organelle development. PleC functions as a membrane-associated protein kinase that transfers phosphate to the response regulator pleD, leading to its activation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=259, Percent_Identity=35.5212355212355, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI145693157, Length=245, Percent_Identity=34.6938775510204, Blast_Score=140, Evalue=4e-34,
Organism=Escherichia coli, GI48994928, Length=233, Percent_Identity=34.7639484978541, Blast_Score=135, Evalue=9e-33,
Organism=Escherichia coli, GI1788713, Length=259, Percent_Identity=35.1351351351351, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI87081816, Length=254, Percent_Identity=32.6771653543307, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1786912, Length=254, Percent_Identity=27.9527559055118, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1788393, Length=267, Percent_Identity=23.9700374531835, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1786600, Length=224, Percent_Identity=29.0178571428571, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1788549, Length=247, Percent_Identity=28.7449392712551, Blast_Score=92, Evalue=2e-19,
Organism=Escherichia coli, GI1786783, Length=259, Percent_Identity=24.3243243243243, Blast_Score=87, Evalue=6e-18,
Organism=Escherichia coli, GI1790436, Length=241, Percent_Identity=25.3112033195021, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87082128, Length=255, Percent_Identity=24.3137254901961, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1790861, Length=247, Percent_Identity=23.4817813765182, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789403, Length=245, Percent_Identity=25.7142857142857, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1790346, Length=228, Percent_Identity=23.6842105263158, Blast_Score=67, Evalue=6e-12,
Organism=Escherichia coli, GI1788279, Length=262, Percent_Identity=24.8091603053435, Blast_Score=64, Evalue=4e-11,
Organism=Escherichia coli, GI1789808, Length=249, Percent_Identity=23.2931726907631, Blast_Score=63, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=206, Percent_Identity=30.0970873786408, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 86696; Mature: 86565

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLDACNAPTEIYADSKSSAQNHKARTKHINPLFDSSYQKLLFIEPWLRRSLPFVIVVF
CCCCCCCCCCHHEECCCCCCHHHHHHHHHCCCCHHCCCCCEEEEECHHHHHCCHHHHHHH
LVVLAAIRFVSIYDLRHTIDKNTRSTITLLAAHIANTIDRDLLAATQKGKAIALSHTYLQ
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHH
NILSNFQNQGLIKTGTVIAIVDQKGNILASSSPEIILGKPLQDFISESIALRSLRKHAGI
HHHHHHHCCCCEEECCEEEEEECCCCEEECCCCCEEECCCHHHHHHHHHHHHHHHHHCCE
MKVTIGKDAPALASFHQTENGKYGVFISEKTQEIYREWRKIFSLNITLLIGTGGVILALL
EEEEECCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHH
YAYYNQIVRARKTDSISEKIQNRIDMAMMRGRCGLWDWNMASGRVYWSRAMYEMLGYIPQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCH
DALLSISQITAIINPNDANFFDLAQELMSGKKKHIDINVPMRHADGHYVWMRIRAEVTDE
HHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCEEECCCCEEEEEEEEECCCC
EELHLVGIAFDISEQRQLAEETAQADLRIRDAIENISESFVLWDSEGRLVMSNSKFCEYA
CCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCHHHH
AIPKQVLQPGIQRATVEAMARPAINEYPLKDDGTGNLTSIRQTADGCWLKINERRTQDGG
CCCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCC
LVCIGTDISELKQQQEKFEDSERRLFSFIQELKRARGNAQQRATEVEKLNKSLKAEKERA
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ESANKAKSEFLANMSHELRTPLNAILGFSDIMLQSTFGPLGSQRYQEYMHDIYNSGNHLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHH
TLINDILDMSKIEAGRFTLDRKNIDLEPIISEAVRTLTPQAQEKNISVSTNIAPELHAEV
HHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHCC
DGRAMKQIFLNLISNAVKFTPSGGSINVCAFKKKNNLVCRIKDTGVGIPQSAIKKLGQPF
CHHHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHCCHH
EQVENQLTKTHTGSGLGLAISRSLLELHKGKLEIISKEMKGTTVTITMPIKQS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEECCCC
>Mature Secondary Structure 
AKLDACNAPTEIYADSKSSAQNHKARTKHINPLFDSSYQKLLFIEPWLRRSLPFVIVVF
CCCCCCCCCHHEECCCCCCHHHHHHHHHCCCCHHCCCCCEEEEECHHHHHCCHHHHHHH
LVVLAAIRFVSIYDLRHTIDKNTRSTITLLAAHIANTIDRDLLAATQKGKAIALSHTYLQ
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHH
NILSNFQNQGLIKTGTVIAIVDQKGNILASSSPEIILGKPLQDFISESIALRSLRKHAGI
HHHHHHHCCCCEEECCEEEEEECCCCEEECCCCCEEECCCHHHHHHHHHHHHHHHHHCCE
MKVTIGKDAPALASFHQTENGKYGVFISEKTQEIYREWRKIFSLNITLLIGTGGVILALL
EEEEECCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHH
YAYYNQIVRARKTDSISEKIQNRIDMAMMRGRCGLWDWNMASGRVYWSRAMYEMLGYIPQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCH
DALLSISQITAIINPNDANFFDLAQELMSGKKKHIDINVPMRHADGHYVWMRIRAEVTDE
HHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCEEECCCCEEEEEEEEECCCC
EELHLVGIAFDISEQRQLAEETAQADLRIRDAIENISESFVLWDSEGRLVMSNSKFCEYA
CCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCHHHH
AIPKQVLQPGIQRATVEAMARPAINEYPLKDDGTGNLTSIRQTADGCWLKINERRTQDGG
CCCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCC
LVCIGTDISELKQQQEKFEDSERRLFSFIQELKRARGNAQQRATEVEKLNKSLKAEKERA
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ESANKAKSEFLANMSHELRTPLNAILGFSDIMLQSTFGPLGSQRYQEYMHDIYNSGNHLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHH
TLINDILDMSKIEAGRFTLDRKNIDLEPIISEAVRTLTPQAQEKNISVSTNIAPELHAEV
HHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHCC
DGRAMKQIFLNLISNAVKFTPSGGSINVCAFKKKNNLVCRIKDTGVGIPQSAIKKLGQPF
CHHHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHCCHH
EQVENQLTKTHTGSGLGLAISRSLLELHKGKLEIISKEMKGTTVTITMPIKQS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8421698; 11259647 [H]