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The map label for this gene is mhpC [H]

Identifier: 46446765

GI number: 46446765

Start: 1358842

End: 1359810

Strand: Direct

Name: mhpC [H]

Synonym: pc1131

Alternate gene names: 46446765

Gene position: 1358842-1359810 (Clockwise)

Preceding gene: 46446762

Following gene: 46446766

Centisome position: 56.28

GC content: 34.88

Gene sequence:

>969_bases
ATGAGAAAATTTCTTTATCGTTTCATTTTTCCAAGTTTTACTATTTTGGCAGTATTATTTACCTTGTACTTTTTTACAGC
TATTTCCTTGTTGGAAGCGGAAAAACAAGACGAGCTAAAAAAAGGAAAATTATTTTGGGAATGGCAAACTCCGCAAGGAA
ATTGGCAAGTCCATTACACTGAACATGGATCTGGCTCTAAACACCTTTTACTTATCCACGGTTTTCGTGCTCATTCTTTT
ACTTGGAGGTACTTAATAGAACCTCTAACACAAGCTGGTTATCATGTTTGGACAATTGATCTAATCGGATATGGATTAAG
TGATAAACCCTTAAATGCTGCGTATGATGCCGATTTCTTTATCGAACAGTTAAAATCTTTTATGGATGCAAAACAAATTT
CTTCCGCTCATTTAATCGGAAGTTCGATGGGAGGTGGCTTAGCTTTAAATTTAACATTAGATTACCCTGAAAAAGTGAGC
TCTTTAACACTAATTAATGCTTTAGGATATCCTCTTGATCTTCCTTTTTATCTTTATTTAACAAGGCACCTTGACCCCTT
ATGGTTTTCCTTTGTTTCTCCGCCAGTCATAAGGATTGGATTGAAGCAAATTGTATTTGATCCAGATACTGTTTCTGAGG
AACAAGTTTTAGCTTATTCTTTCCCATATCAATTTCCAGGCGGAACACAGGCGTCCTTAACAACCTTAAAACAATTTGAC
AAACAAAAGTTAGTCGATTTAAGCCAACGATACCATTCTCTCAAACATCCTTTGTTAATTATTTGGGGAGATAAAGACAA
ATTAATTCCCATTACCCATTATGAAAGATTTGTTAAAGAATTTCCACAAGCCGATTGCCTTTTAATTCCTAACTGTGGCC
ATATTCCTCATGAAGAAAAACCCATTTTAGTAACAGAAACGATTTTGGAGTTTCTTGGCAAACATATTGATCGTTCAAAT
AGCGATTAA

Upstream 100 bases:

>100_bases
AGTTTGCACTCTCTGTCGAAAGTGAAATCGCAAGAAAGGCTTTATCCGATTTAGCCACCCTAAGTATGATTAACTAAAGA
TTTTTGTTTGTGAGCATAAA

Downstream 100 bases:

>100_bases
AATCTTTCTATTTGCTGAGTTATTTTCCAAACTTAATCATTGAAGTTTTATTTCACTAATAAAAAAGGCATATTGAATAT
GTCCATTCAATAAACGTTTG

Product: hypothetical protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MRKFLYRFIFPSFTILAVLFTLYFFTAISLLEAEKQDELKKGKLFWEWQTPQGNWQVHYTEHGSGSKHLLLIHGFRAHSF
TWRYLIEPLTQAGYHVWTIDLIGYGLSDKPLNAAYDADFFIEQLKSFMDAKQISSAHLIGSSMGGGLALNLTLDYPEKVS
SLTLINALGYPLDLPFYLYLTRHLDPLWFSFVSPPVIRIGLKQIVFDPDTVSEEQVLAYSFPYQFPGGTQASLTTLKQFD
KQKLVDLSQRYHSLKHPLLIIWGDKDKLIPITHYERFVKEFPQADCLLIPNCGHIPHEEKPILVTETILEFLGKHIDRSN
SD

Sequences:

>Translated_322_residues
MRKFLYRFIFPSFTILAVLFTLYFFTAISLLEAEKQDELKKGKLFWEWQTPQGNWQVHYTEHGSGSKHLLLIHGFRAHSF
TWRYLIEPLTQAGYHVWTIDLIGYGLSDKPLNAAYDADFFIEQLKSFMDAKQISSAHLIGSSMGGGLALNLTLDYPEKVS
SLTLINALGYPLDLPFYLYLTRHLDPLWFSFVSPPVIRIGLKQIVFDPDTVSEEQVLAYSFPYQFPGGTQASLTTLKQFD
KQKLVDLSQRYHSLKHPLLIIWGDKDKLIPITHYERFVKEFPQADCLLIPNCGHIPHEEKPILVTETILEFLGKHIDRSN
SD
>Mature_322_residues
MRKFLYRFIFPSFTILAVLFTLYFFTAISLLEAEKQDELKKGKLFWEWQTPQGNWQVHYTEHGSGSKHLLLIHGFRAHSF
TWRYLIEPLTQAGYHVWTIDLIGYGLSDKPLNAAYDADFFIEQLKSFMDAKQISSAHLIGSSMGGGLALNLTLDYPEKVS
SLTLINALGYPLDLPFYLYLTRHLDPLWFSFVSPPVIRIGLKQIVFDPDTVSEEQVLAYSFPYQFPGGTQASLTTLKQFD
KQKLVDLSQRYHSLKHPLLIIWGDKDKLIPITHYERFVKEFPQADCLLIPNCGHIPHEEKPILVTETILEFLGKHIDRSN
SD

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mhpC alpha/beta hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=291, Percent_Identity=25.4295532646048, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI189027141, Length=306, Percent_Identity=24.5098039215686, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI27597073, Length=112, Percent_Identity=36.6071428571429, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI218777840, Length=283, Percent_Identity=22.6148409893993, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI13376164, Length=283, Percent_Identity=22.6148409893993, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87081721, Length=266, Percent_Identity=25.9398496240602, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324392, Length=283, Percent_Identity=24.7349823321555, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 37116; Mature: 37116

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKFLYRFIFPSFTILAVLFTLYFFTAISLLEAEKQDELKKGKLFWEWQTPQGNWQVHYT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEE
EHGSGSKHLLLIHGFRAHSFTWRYLIEPLTQAGYHVWTIDLIGYGLSDKPLNAAYDADFF
ECCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHH
IEQLKSFMDAKQISSAHLIGSSMGGGLALNLTLDYPEKVSSLTLINALGYPLDLPFYLYL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHH
TRHLDPLWFSFVSPPVIRIGLKQIVFDPDTVSEEQVLAYSFPYQFPGGTQASLTTLKQFD
HHHCCHHHHHHCCCHHHHHHHHHHEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
KQKLVDLSQRYHSLKHPLLIIWGDKDKLIPITHYERFVKEFPQADCLLIPNCGHIPHEEK
HHHHHHHHHHHHHHCCCEEEEECCCCCEEEHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
PILVTETILEFLGKHIDRSNSD
CEEHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRKFLYRFIFPSFTILAVLFTLYFFTAISLLEAEKQDELKKGKLFWEWQTPQGNWQVHYT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEE
EHGSGSKHLLLIHGFRAHSFTWRYLIEPLTQAGYHVWTIDLIGYGLSDKPLNAAYDADFF
ECCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHH
IEQLKSFMDAKQISSAHLIGSSMGGGLALNLTLDYPEKVSSLTLINALGYPLDLPFYLYL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHH
TRHLDPLWFSFVSPPVIRIGLKQIVFDPDTVSEEQVLAYSFPYQFPGGTQASLTTLKQFD
HHHCCHHHHHHCCCHHHHHHHHHHEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
KQKLVDLSQRYHSLKHPLLIIWGDKDKLIPITHYERFVKEFPQADCLLIPNCGHIPHEEK
HHHHHHHHHHHHHHCCCEEEEECCCCCEEEHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
PILVTETILEFLGKHIDRSNSD
CEEHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA