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The map label for this gene is holB [H]

Identifier: 46446706

GI number: 46446706

Start: 1280171

End: 1281280

Strand: Reverse

Name: holB [H]

Synonym: pc1072

Alternate gene names: 46446706

Gene position: 1281280-1280171 (Counterclockwise)

Preceding gene: 46446707

Following gene: 46446705

Centisome position: 53.07

GC content: 34.14

Gene sequence:

>1110_bases
TTGAACAAATTTCCCTCTTCTTTTTCTTTTCTCGTTGGTAATGATCCTATTAAAATTTATCTGCAAAGAATGCTTATAAA
AAAAGCTATCGGAAATTCTTTACTTTTTGCTGGACCAAATGGCGTTGGGAAAGGGCTGTTTGCCTATGCATTGGCGAGTC
AATTAATCTGTGAACAAGAAGAAAAAAATAAGTATAAAATAGAAAATGGGTTGCATCCTGATATTCATATTTATCGTCCT
GAAGGAAAGTTAGGCCTACATGGTATTCAATCGCTTAGAGAATTAAGTGAAGAAGTTCACTTACCTCCTTATGAAGCCTC
TTGGAAGATTTTTATTATTCATGATGCTGAACGCATGTTGAGTGTTAGTGCTAACACGCTATTAAAAACTTTTGAAGAGC
CTCCACCAAAAACGGTGATCATTCTACTAAGTCAATCACAAGCAGCGCTTTTACCAACAATTGTCTCTCGTTGTCGAACC
CTTCATTTTCAACCAATTCATGCAAGTGAAATTGAAAAAGTATTAGTCGAACGAATGTATTTAGAAACTGAATTTGCTCA
AAACATAGCAAGCTTATCACAAGGATCTTTAGGAAGAGCTGTTCAATTGATTCAAAAAAAAGGGGATGAAAGTCGTATTT
TTCTATTGAATACACTTTCCCAGTTGCCTTTAACGAGTTATAAAGAATTTACTCAAATTGTTGGAGAATTGGTGGCACAA
ATTGAACTTTCTAAAAAACTCTTTGAAGAAAAAGCCAAAGAGGAATTTCTAAAGTTATTTAGTGAGCAATTATCCACAGC
GCATCAACAACATCTTGAAAAAGAAATCGAGGGAAGGTGCACGCAGATTTTTCTACAAGACGTAGAATCTTTATTTGATC
AACTTTTCGCTTGGTATCGGGATTTGCATCTCTTACAACTGACTCAAAACAAATCATTGTTAATTCACAGCGATTATGCT
GAAAGCATTGAGCAGATATTACAAAGAGGAAATCTTTTACCCTTAGAAATAGTTCAAAAAAGTATTGAAGAAGCCCGTCT
ATCACTTCAACGTTCGACATCTTTAAATATTTGCCTAGAAAATTTATTTTTAAAATTGGCTTGGATTTAA

Upstream 100 bases:

>100_bases
AGCGAATATATCGTATTGATGCTAATGAAAGTCAATCAAAAGTTTTACAAAAAGCGCTTGAATTTTTAGAAGAGCAGTGG
TCTGACTCGGAGTTGAAAAC

Downstream 100 bases:

>100_bases
AACCTTTGACCTTTTTTCCATCATTTCATACCTAGAGAAAGTTTTTAAGTTAAAAGTTTGATTATAGAACTTTAATGTTT
TTTGTTAAAATTTATTTTAA

Product: putative DNA polymerase III, delta' subunit, holB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MNKFPSSFSFLVGNDPIKIYLQRMLIKKAIGNSLLFAGPNGVGKGLFAYALASQLICEQEEKNKYKIENGLHPDIHIYRP
EGKLGLHGIQSLRELSEEVHLPPYEASWKIFIIHDAERMLSVSANTLLKTFEEPPPKTVIILLSQSQAALLPTIVSRCRT
LHFQPIHASEIEKVLVERMYLETEFAQNIASLSQGSLGRAVQLIQKKGDESRIFLLNTLSQLPLTSYKEFTQIVGELVAQ
IELSKKLFEEKAKEEFLKLFSEQLSTAHQQHLEKEIEGRCTQIFLQDVESLFDQLFAWYRDLHLLQLTQNKSLLIHSDYA
ESIEQILQRGNLLPLEIVQKSIEEARLSLQRSTSLNICLENLFLKLAWI

Sequences:

>Translated_369_residues
MNKFPSSFSFLVGNDPIKIYLQRMLIKKAIGNSLLFAGPNGVGKGLFAYALASQLICEQEEKNKYKIENGLHPDIHIYRP
EGKLGLHGIQSLRELSEEVHLPPYEASWKIFIIHDAERMLSVSANTLLKTFEEPPPKTVIILLSQSQAALLPTIVSRCRT
LHFQPIHASEIEKVLVERMYLETEFAQNIASLSQGSLGRAVQLIQKKGDESRIFLLNTLSQLPLTSYKEFTQIVGELVAQ
IELSKKLFEEKAKEEFLKLFSEQLSTAHQQHLEKEIEGRCTQIFLQDVESLFDQLFAWYRDLHLLQLTQNKSLLIHSDYA
ESIEQILQRGNLLPLEIVQKSIEEARLSLQRSTSLNICLENLFLKLAWI
>Mature_369_residues
MNKFPSSFSFLVGNDPIKIYLQRMLIKKAIGNSLLFAGPNGVGKGLFAYALASQLICEQEEKNKYKIENGLHPDIHIYRP
EGKLGLHGIQSLRELSEEVHLPPYEASWKIFIIHDAERMLSVSANTLLKTFEEPPPKTVIILLSQSQAALLPTIVSRCRT
LHFQPIHASEIEKVLVERMYLETEFAQNIASLSQGSLGRAVQLIQKKGDESRIFLLNTLSQLPLTSYKEFTQIVGELVAQ
IELSKKLFEEKAKEEFLKLFSEQLSTAHQQHLEKEIEGRCTQIFLQDVESLFDQLFAWYRDLHLLQLTQNKSLLIHSDYA
ESIEQILQRGNLLPLEIVQKSIEEARLSLQRSTSLNICLENLFLKLAWI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=216, Percent_Identity=29.1666666666667, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787341, Length=197, Percent_Identity=28.4263959390863, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42167; Mature: 42167

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFPSSFSFLVGNDPIKIYLQRMLIKKAIGNSLLFAGPNGVGKGLFAYALASQLICEQE
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
EKNKYKIENGLHPDIHIYRPEGKLGLHGIQSLRELSEEVHLPPYEASWKIFIIHDAERML
HCCCEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHH
SVSANTLLKTFEEPPPKTVIILLSQSQAALLPTIVSRCRTLHFQPIHASEIEKVLVERMY
HHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LETEFAQNIASLSQGSLGRAVQLIQKKGDESRIFLLNTLSQLPLTSYKEFTQIVGELVAQ
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHH
IELSKKLFEEKAKEEFLKLFSEQLSTAHQQHLEKEIEGRCTQIFLQDVESLFDQLFAWYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLHLLQLTQNKSLLIHSDYAESIEQILQRGNLLPLEIVQKSIEEARLSLQRSTSLNICLE
HHHHEEEECCCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
NLFLKLAWI
HHHHHHHCC
>Mature Secondary Structure
MNKFPSSFSFLVGNDPIKIYLQRMLIKKAIGNSLLFAGPNGVGKGLFAYALASQLICEQE
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
EKNKYKIENGLHPDIHIYRPEGKLGLHGIQSLRELSEEVHLPPYEASWKIFIIHDAERML
HCCCEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHH
SVSANTLLKTFEEPPPKTVIILLSQSQAALLPTIVSRCRTLHFQPIHASEIEKVLVERMY
HHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LETEFAQNIASLSQGSLGRAVQLIQKKGDESRIFLLNTLSQLPLTSYKEFTQIVGELVAQ
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHH
IELSKKLFEEKAKEEFLKLFSEQLSTAHQQHLEKEIEGRCTQIFLQDVESLFDQLFAWYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLHLLQLTQNKSLLIHSDYAESIEQILQRGNLLPLEIVQKSIEEARLSLQRSTSLNICLE
HHHHEEEECCCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
NLFLKLAWI
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]