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The map label for this gene is menE [H]

Identifier: 46446696

GI number: 46446696

Start: 1263309

End: 1264442

Strand: Reverse

Name: menE [H]

Synonym: pc1062

Alternate gene names: 46446696

Gene position: 1264442-1263309 (Counterclockwise)

Preceding gene: 46446697

Following gene: 46446692

Centisome position: 52.37

GC content: 36.95

Gene sequence:

>1134_bases
ATGAAAATTGATTGGATAAGTTCCGAATCAAGAGTTTTTTTAAATCCCAGTTATGGCGTTGAAAAACAACAACTTTACTT
AAAAATACTCAAGAAAACTAAAAATCTTGATAGTCACTTTTGGCTTTCTACGTCAGGTTCCACTAGCCAAAAATGGGTTG
GTTTATCCAAAAAAGCAATTTTAGCTTCCGCTGAATCTGTGAATAAACATATTGAAGCCAAATTTTGTGATCGTTGGGTG
ACTGCCTTACCCGATTTTCATGTCGGAGGACTTGGAATTTGGGCTAGAGCCTTTTTGAGTGACTCTCAGGTTTTTGATTT
TAAAACAATTCAAAATGGGAAGTGGGATCCTAAAATTTTCTGGAGTTTTGTAAGGGAAATGAAAGGAACTTTAACGGCTT
TAGTCCCGACTCAAATTTATGACTTAGTGCAATTAGCTTTACCAGCTCCCAATAGTTTAAGGGCCGTTATAGTAGGAGGT
GGTAGGTTGTTACCTGACTTATATAAGAAAGCTGTTGAATTAGGGTGGCCTTTAATGCCAAGTTATGGTTTGACAGAATG
CTGTTCTCAAGTCGCGACAGCTACCCTTGGAAGCTGGCAGTTTTCCACTTATCCCTCTTTGCAAATTCTAACTCACATTA
GTGTGAGAGAAAGAGATGGACATTTGGAATATAAAAGCCCTTCTTTGCTTACCTGTTATGCCTATTTTGAGCAAGATCAA
ATTTATTTACACGACCCAAAAGTTGAGGGGTGGTTTAGATGTGGGGATCTAGGCAATCTACAAGGGAATATATTGAATGT
GAAAGGTCGCTCTGATCATTTGCTTAAAATTGGGGGGGAAAGTGTTGATTTGAGTCAATTAGAGGCTCATTTGGAATCAA
TTTTACTAAAAAATTCTTCTGCTGGAATGGCTGTTTTAGTGGCGGTACCAGATGAAAGATTAGGAAATATTTTATATTTA
GCTGTTGAACAAAATAAGCGGGAGATATTAAAGCCATTAATAGGCCTTTATCAGCAAACAGTTCTTCCTTTTGAGCGGAT
TCGAAACGTTATTGCTGTTGAAAATATTCCCCGTTCACCTCTAGGAAAAATATTAAAAAGCGAGCTGATTAAATTAATAA
CTTTGAACGAATAA

Upstream 100 bases:

>100_bases
TAGTTTTGCGCAAAGTATTTTGTTAATTTTTAGTTTTGGTTTATTGTTGGCCGTAGCTTGTTTGATGCGTATAGCCGAAT
GAACGTTGGAAATAATTTGT

Downstream 100 bases:

>100_bases
ACAAATCCTCAAAAATATAATTCTAATTGGCTTTTAATTTCATTGGGGATTAGAAGTTTATCCTTACTTTGTCGATTACA
AAATACGTGAATGGTTTTGA

Product: putative o-succinylbenzoate-CoA ligase, menE

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKIDWISSESRVFLNPSYGVEKQQLYLKILKKTKNLDSHFWLSTSGSTSQKWVGLSKKAILASAESVNKHIEAKFCDRWV
TALPDFHVGGLGIWARAFLSDSQVFDFKTIQNGKWDPKIFWSFVREMKGTLTALVPTQIYDLVQLALPAPNSLRAVIVGG
GRLLPDLYKKAVELGWPLMPSYGLTECCSQVATATLGSWQFSTYPSLQILTHISVRERDGHLEYKSPSLLTCYAYFEQDQ
IYLHDPKVEGWFRCGDLGNLQGNILNVKGRSDHLLKIGGESVDLSQLEAHLESILLKNSSAGMAVLVAVPDERLGNILYL
AVEQNKREILKPLIGLYQQTVLPFERIRNVIAVENIPRSPLGKILKSELIKLITLNE

Sequences:

>Translated_377_residues
MKIDWISSESRVFLNPSYGVEKQQLYLKILKKTKNLDSHFWLSTSGSTSQKWVGLSKKAILASAESVNKHIEAKFCDRWV
TALPDFHVGGLGIWARAFLSDSQVFDFKTIQNGKWDPKIFWSFVREMKGTLTALVPTQIYDLVQLALPAPNSLRAVIVGG
GRLLPDLYKKAVELGWPLMPSYGLTECCSQVATATLGSWQFSTYPSLQILTHISVRERDGHLEYKSPSLLTCYAYFEQDQ
IYLHDPKVEGWFRCGDLGNLQGNILNVKGRSDHLLKIGGESVDLSQLEAHLESILLKNSSAGMAVLVAVPDERLGNILYL
AVEQNKREILKPLIGLYQQTVLPFERIRNVIAVENIPRSPLGKILKSELIKLITLNE
>Mature_377_residues
MKIDWISSESRVFLNPSYGVEKQQLYLKILKKTKNLDSHFWLSTSGSTSQKWVGLSKKAILASAESVNKHIEAKFCDRWV
TALPDFHVGGLGIWARAFLSDSQVFDFKTIQNGKWDPKIFWSFVREMKGTLTALVPTQIYDLVQLALPAPNSLRAVIVGG
GRLLPDLYKKAVELGWPLMPSYGLTECCSQVATATLGSWQFSTYPSLQILTHISVRERDGHLEYKSPSLLTCYAYFEQDQ
IYLHDPKVEGWFRCGDLGNLQGNILNVKGRSDHLLKIGGESVDLSQLEAHLESILLKNSSAGMAVLVAVPDERLGNILYL
AVEQNKREILKPLIGLYQQTVLPFERIRNVIAVENIPRSPLGKILKSELIKLITLNE

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6319699, Length=357, Percent_Identity=25.4901960784314, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 42358; Mature: 42358

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDWISSESRVFLNPSYGVEKQQLYLKILKKTKNLDSHFWLSTSGSTSQKWVGLSKKAI
CEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCCCCHHHH
LASAESVNKHIEAKFCDRWVTALPDFHVGGLGIWARAFLSDSQVFDFKTIQNGKWDPKIF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHH
WSFVREMKGTLTALVPTQIYDLVQLALPAPNSLRAVIVGGGRLLPDLYKKAVELGWPLMP
HHHHHHHHCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCC
SYGLTECCSQVATATLGSWQFSTYPSLQILTHISVRERDGHLEYKSPSLLTCYAYFEQDQ
CCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCEEECCCCEEEEEEEECCCE
IYLHDPKVEGWFRCGDLGNLQGNILNVKGRSDHLLKIGGESVDLSQLEAHLESILLKNSS
EEEECCCCCCEEEECCCCCCCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCC
AGMAVLVAVPDERLGNILYLAVEQNKREILKPLIGLYQQTVLPFERIRNVIAVENIPRSP
CCEEEEEECCCHHCCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCH
LGKILKSELIKLITLNE
HHHHHHHHHHHHEECCC
>Mature Secondary Structure
MKIDWISSESRVFLNPSYGVEKQQLYLKILKKTKNLDSHFWLSTSGSTSQKWVGLSKKAI
CEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCCCCHHHH
LASAESVNKHIEAKFCDRWVTALPDFHVGGLGIWARAFLSDSQVFDFKTIQNGKWDPKIF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHH
WSFVREMKGTLTALVPTQIYDLVQLALPAPNSLRAVIVGGGRLLPDLYKKAVELGWPLMP
HHHHHHHHCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCC
SYGLTECCSQVATATLGSWQFSTYPSLQILTHISVRERDGHLEYKSPSLLTCYAYFEQDQ
CCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCEEECCCCEEEEEEEECCCE
IYLHDPKVEGWFRCGDLGNLQGNILNVKGRSDHLLKIGGESVDLSQLEAHLESILLKNSS
EEEECCCCCCEEEECCCCCCCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCC
AGMAVLVAVPDERLGNILYLAVEQNKREILKPLIGLYQQTVLPFERIRNVIAVENIPRSP
CCEEEEEECCCHHCCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCH
LGKILKSELIKLITLNE
HHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]