Click here to switch to the map view.

The map label for this gene is aspS

Identifier: 46446018

GI number: 46446018

Start: 509561

End: 511360

Strand: Reverse

Name: aspS

Synonym: pc0384

Alternate gene names: 46446018

Gene position: 511360-509561 (Counterclockwise)

Preceding gene: 46446019

Following gene: 46446017

Centisome position: 21.18

GC content: 36.83

Gene sequence:

>1800_bases
ATGAATCTTATGATGTTTGATTACCGTAGATCCCATACGTGTGGACAATTACGTAAAGAAAAAGTGAATTCTCAAGTGAC
CTTATCTGGATGGGTTAATCGCCGACGAGATCACGGAGGTCTTATTTTTATTGATTTAAGGGATCGTTTTGGCTTAACCC
AGCTTGTTTTCGATCCAATTAAAAGTCCAACTGCCCATTTAGCGGCTGAGAAATTACGCTCTGAATGGGTTATTTCCGTT
AAAGGAACAGTGATTCCTAGACAAGAAGGAATGACAAATCCGAAGCTTCCCACTGGAGAAATCGAGATTATGGTCCATGA
AATGGACATTCTCTCAAAATCGAAAACACCCCCATTTTCGGTTTCTGATGACCTTATTGAGGTTAACGAAGAGCTAAGAT
TAAAATATCGTTATCTAGATATTCGGCGAGGAGATGTAGCAAAGAAATTAATCACTCGCCATCAAGCTATGCTTGCAGTT
AGAAATTATTTAAATAACCAAGGATTCTTAGAAATTTCTACTCCAATTTTAGGTAAATCTACACCTGAAGGAGCACGCGA
TTATCTTGTTCCTTCCCGTGTCTATCCAGGAAATTTTTACGCACTACCGCAATCTCCACAGATTTTTAAACAATTGTTGA
TGATTTCTGGAATGGATCGTTATTTCCAAATAGCGCAATGTTTTAGAGATGAAGATTTGCGAGCTGATAGACAACCAGAA
TTCACGCAAATCGATATGGAAATGAGTTTTGGTACGCCTGAAGATCTAATGAATATTGTAGAAGATTTGATAAAAACAGT
TTTTAAAACCTGTTCTAACATCGATGTACCAACACCATTTAAACGCTTGTCACATGCAATTTGTATGGAAGAATATGGAT
GTGATCGTCCCGATCTTCGCTTTGGTATGAAACTGCACAATTTAAATCATCTTGCTGCCCAAACAACATTTTCGGTCTTT
TTAGATCAAATCAGAGAAAATGGTCTTGTGAAAGGATTTTGTATCAAAGGTGGGGCCGATTTTTCACGAAAAACAATCGA
TGAATATACTGAGTTTGTAGGACGCCTAGGGGTAAAAGGATTGGCTTGGATTAAAAGACAAGAAAATGGTCTTAATTCAA
GTATTGTCAAATTCTTTCCTGAGTCTATTCATCAACAGTTAATTGAAGAAATGGAAATGGAAGTAGGAGATATCATCTTT
ATGATTGCTAATACTCCTTCTAAAACAAATCAAGCACTTGATCATTTAAGGCGTAAAATCGCAAGAGATCGCAATCTTGT
CGATCCGCATCATTATGAATTTTTATGGGTAACCGATTTCCCTCTATTTAGCTGGAATGAAGAAGAAAAAAGACTTCAGA
GCGAACATCATCCATTCACTTCTCCACATTTAGAAGACCTTCATTTAATGGAAACTAACCCCTTGAAAATGCGTTCCTCA
GGTTATGACATTGTTCTCAATGGTTATGAAATTGGGGGAGGTTCGCAACGTATTCACAATAGTGATCTCCAACAAAAGAT
TTTTGAACGGTTAAAATTTTCTCCAGAAGAACTCGAAACAAAATTTGGATTTTTCTTAGAAGCCCTTAATTATGGTACCC
CCCCTCATTTGGGAATTGCTTTAGGATTGGATCGTATTATCATGATTTTAACCCAAACAGAAAATATTCGTGATGTTATT
GCATTTCCTAAAACCCAGAAAGCAAGTGATCTAATGATAGAATGCCCTTCCCCGGTAGCTAATGAACAACTTAAAGAATT
AGAAATACGTGTACCTGACTCTCAATTTTCTTGGACTTAA

Upstream 100 bases:

>100_bases
AACGCAATTTTTCGTTGAAAAGCTACATAAGTCTCTCTACGAAGCACAAAATTTAAAAAACGAGCCATGAATAATGCCGA
AAAAATGAAAAACCTAGTGG

Downstream 100 bases:

>100_bases
TTCGTGAGATTGCCTACAGTAATATTCTTTCAAATGGAGGTCTATTTATGTTCAAACAGACAAAATTATTAGCTCTAGCT
AGCTTTGGAATGTTTATCGG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MNLMMFDYRRSHTCGQLRKEKVNSQVTLSGWVNRRRDHGGLIFIDLRDRFGLTQLVFDPIKSPTAHLAAEKLRSEWVISV
KGTVIPRQEGMTNPKLPTGEIEIMVHEMDILSKSKTPPFSVSDDLIEVNEELRLKYRYLDIRRGDVAKKLITRHQAMLAV
RNYLNNQGFLEISTPILGKSTPEGARDYLVPSRVYPGNFYALPQSPQIFKQLLMISGMDRYFQIAQCFRDEDLRADRQPE
FTQIDMEMSFGTPEDLMNIVEDLIKTVFKTCSNIDVPTPFKRLSHAICMEEYGCDRPDLRFGMKLHNLNHLAAQTTFSVF
LDQIRENGLVKGFCIKGGADFSRKTIDEYTEFVGRLGVKGLAWIKRQENGLNSSIVKFFPESIHQQLIEEMEMEVGDIIF
MIANTPSKTNQALDHLRRKIARDRNLVDPHHYEFLWVTDFPLFSWNEEEKRLQSEHHPFTSPHLEDLHLMETNPLKMRSS
GYDIVLNGYEIGGGSQRIHNSDLQQKIFERLKFSPEELETKFGFFLEALNYGTPPHLGIALGLDRIIMILTQTENIRDVI
AFPKTQKASDLMIECPSPVANEQLKELEIRVPDSQFSWT

Sequences:

>Translated_599_residues
MNLMMFDYRRSHTCGQLRKEKVNSQVTLSGWVNRRRDHGGLIFIDLRDRFGLTQLVFDPIKSPTAHLAAEKLRSEWVISV
KGTVIPRQEGMTNPKLPTGEIEIMVHEMDILSKSKTPPFSVSDDLIEVNEELRLKYRYLDIRRGDVAKKLITRHQAMLAV
RNYLNNQGFLEISTPILGKSTPEGARDYLVPSRVYPGNFYALPQSPQIFKQLLMISGMDRYFQIAQCFRDEDLRADRQPE
FTQIDMEMSFGTPEDLMNIVEDLIKTVFKTCSNIDVPTPFKRLSHAICMEEYGCDRPDLRFGMKLHNLNHLAAQTTFSVF
LDQIRENGLVKGFCIKGGADFSRKTIDEYTEFVGRLGVKGLAWIKRQENGLNSSIVKFFPESIHQQLIEEMEMEVGDIIF
MIANTPSKTNQALDHLRRKIARDRNLVDPHHYEFLWVTDFPLFSWNEEEKRLQSEHHPFTSPHLEDLHLMETNPLKMRSS
GYDIVLNGYEIGGGSQRIHNSDLQQKIFERLKFSPEELETKFGFFLEALNYGTPPHLGIALGLDRIIMILTQTENIRDVI
AFPKTQKASDLMIECPSPVANEQLKELEIRVPDSQFSWT
>Mature_599_residues
MNLMMFDYRRSHTCGQLRKEKVNSQVTLSGWVNRRRDHGGLIFIDLRDRFGLTQLVFDPIKSPTAHLAAEKLRSEWVISV
KGTVIPRQEGMTNPKLPTGEIEIMVHEMDILSKSKTPPFSVSDDLIEVNEELRLKYRYLDIRRGDVAKKLITRHQAMLAV
RNYLNNQGFLEISTPILGKSTPEGARDYLVPSRVYPGNFYALPQSPQIFKQLLMISGMDRYFQIAQCFRDEDLRADRQPE
FTQIDMEMSFGTPEDLMNIVEDLIKTVFKTCSNIDVPTPFKRLSHAICMEEYGCDRPDLRFGMKLHNLNHLAAQTTFSVF
LDQIRENGLVKGFCIKGGADFSRKTIDEYTEFVGRLGVKGLAWIKRQENGLNSSIVKFFPESIHQQLIEEMEMEVGDIIF
MIANTPSKTNQALDHLRRKIARDRNLVDPHHYEFLWVTDFPLFSWNEEEKRLQSEHHPFTSPHLEDLHLMETNPLKMRSS
GYDIVLNGYEIGGGSQRIHNSDLQQKIFERLKFSPEELETKFGFFLEALNYGTPPHLGIALGLDRIIMILTQTENIRDVI
AFPKTQKASDLMIECPSPVANEQLKELEIRVPDSQFSWT

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=594, Percent_Identity=43.6026936026936, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI5031815, Length=326, Percent_Identity=26.9938650306748, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI194272210, Length=326, Percent_Identity=26.9938650306748, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI45439306, Length=269, Percent_Identity=26.3940520446097, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI4758762, Length=247, Percent_Identity=27.9352226720648, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788173, Length=589, Percent_Identity=48.2173174872666, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI1790571, Length=312, Percent_Identity=25.6410256410256, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789256, Length=314, Percent_Identity=25.796178343949, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32566633, Length=603, Percent_Identity=34.9917081260365, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI71994340, Length=191, Percent_Identity=28.2722513089005, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=191, Percent_Identity=28.2722513089005, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=191, Percent_Identity=28.2722513089005, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=648, Percent_Identity=32.5617283950617, Blast_Score=280, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6321807, Length=252, Percent_Identity=27.7777777777778, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=260, Percent_Identity=26.1538461538462, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323011, Length=280, Percent_Identity=30, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24584738, Length=601, Percent_Identity=36.4392678868552, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24640849, Length=300, Percent_Identity=28, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640851, Length=300, Percent_Identity=28, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136276, Length=282, Percent_Identity=26.5957446808511, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921528, Length=234, Percent_Identity=29.0598290598291, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_PARUW (Q6ME91)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007383.1
- ProteinModelPortal:   Q6ME91
- SMR:   Q6ME91
- STRING:   Q6ME91
- GeneID:   2779805
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0384
- NMPDR:   fig|264201.1.peg.384
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   Q6ME91
- ProtClustDB:   PRK00476
- BioCyc:   CPRO264201:PC0384-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 69121; Mature: 69121

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLMMFDYRRSHTCGQLRKEKVNSQVTLSGWVNRRRDHGGLIFIDLRDRFGLTQLVFDPI
CCCEEECCCCCCHHHHHHHHHCCCEEEEEHHHHCCCCCCCEEEEEECCCCCHHHHHHHHC
KSPTAHLAAEKLRSEWVISVKGTVIPRQEGMTNPKLPTGEIEIMVHEMDILSKSKTPPFS
CCCHHHHHHHHHHCCEEEEECCEEEECCCCCCCCCCCCCCEEEEEEEHHHHHCCCCCCCC
VSDDLIEVNEELRLKYRYLDIRRGDVAKKLITRHQAMLAVRNYLNNQGFLEISTPILGKS
CCCHHHHCCHHHEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
TPEGARDYLVPSRVYPGNFYALPQSPQIFKQLLMISGMDRYFQIAQCFRDEDLRADRQPE
CCCCCHHCCCCCEECCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
FTQIDMEMSFGTPEDLMNIVEDLIKTVFKTCSNIDVPTPFKRLSHAICMEEYGCDRPDLR
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHH
FGMKLHNLNHLAAQTTFSVFLDQIRENGLVKGFCIKGGADFSRKTIDEYTEFVGRLGVKG
HCHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCH
LAWIKRQENGLNSSIVKFFPESIHQQLIEEMEMEVGDIIFMIANTPSKTNQALDHLRRKI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHH
ARDRNLVDPHHYEFLWVTDFPLFSWNEEEKRLQSEHHPFTSPHLEDLHLMETNPLKMRSS
HHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEECC
GYDIVLNGYEIGGGSQRIHNSDLQQKIFERLKFSPEELETKFGFFLEALNYGTPPHLGIA
CCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHH
LGLDRIIMILTQTENIRDVIAFPKTQKASDLMIECPSPVANEQLKELEIRVPDSQFSWT
HHHHHHEEEEECCCCHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHEEEECCCCCCCCC
>Mature Secondary Structure
MNLMMFDYRRSHTCGQLRKEKVNSQVTLSGWVNRRRDHGGLIFIDLRDRFGLTQLVFDPI
CCCEEECCCCCCHHHHHHHHHCCCEEEEEHHHHCCCCCCCEEEEEECCCCCHHHHHHHHC
KSPTAHLAAEKLRSEWVISVKGTVIPRQEGMTNPKLPTGEIEIMVHEMDILSKSKTPPFS
CCCHHHHHHHHHHCCEEEEECCEEEECCCCCCCCCCCCCCEEEEEEEHHHHHCCCCCCCC
VSDDLIEVNEELRLKYRYLDIRRGDVAKKLITRHQAMLAVRNYLNNQGFLEISTPILGKS
CCCHHHHCCHHHEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
TPEGARDYLVPSRVYPGNFYALPQSPQIFKQLLMISGMDRYFQIAQCFRDEDLRADRQPE
CCCCCHHCCCCCEECCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
FTQIDMEMSFGTPEDLMNIVEDLIKTVFKTCSNIDVPTPFKRLSHAICMEEYGCDRPDLR
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHH
FGMKLHNLNHLAAQTTFSVFLDQIRENGLVKGFCIKGGADFSRKTIDEYTEFVGRLGVKG
HCHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCH
LAWIKRQENGLNSSIVKFFPESIHQQLIEEMEMEVGDIIFMIANTPSKTNQALDHLRRKI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHH
ARDRNLVDPHHYEFLWVTDFPLFSWNEEEKRLQSEHHPFTSPHLEDLHLMETNPLKMRSS
HHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEECC
GYDIVLNGYEIGGGSQRIHNSDLQQKIFERLKFSPEELETKFGFFLEALNYGTPPHLGIA
CCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHH
LGLDRIIMILTQTENIRDVIAFPKTQKASDLMIECPSPVANEQLKELEIRVPDSQFSWT
HHHHHHEEEEECCCCHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA