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The map label for this gene is hisS

Identifier: 46446019

GI number: 46446019

Start: 511526

End: 512800

Strand: Reverse

Name: hisS

Synonym: pc0385

Alternate gene names: 46446019

Gene position: 512800-511526 (Counterclockwise)

Preceding gene: 46446020

Following gene: 46446018

Centisome position: 21.24

GC content: 35.84

Gene sequence:

>1275_bases
ATGAAATTTTCAATTCCCCCAGGAGTTTTTGATATCATTCCAACAAATACCCAAGAAATTTGGAAGAGTTCTTATTTATG
GGATTTTGTTGAACGAACCATTAGAAAAACAGTACGAGATTATGGTTATCAAGAAATTCGAACTCCTTTATTTGAACGAA
CAGAGCTTTTTCAAAGAAGTGTGGGAGAAACATCTGATATTATTTCTAAAGAAATGTATACGTTTGAAGATAAAGGTGGG
CGTTCTATGTCGCTTCGCCCAGAAGGAACAGCGCCTGTCATGCGTTCTTTCATTGAAAATCAATTGCATACAATCGCTCC
GTTACAAAAACTTTTTTATATTGCTCCTATGTTTCGCTACGAAAGAGCTCAAGCCGGACGATATCGACAACATCATCAAT
TTGGAGCCGAGGCTATTGGTAATAGTTCGCCCGAACAAGATGCTGAATTGATTGATTTACTTTATACGCTTTATAAACGC
CTAGGACTTCAAAACTTGAGTTTGAATATTAACTCTATTGGAACTATTTCTTGCAGACATACATTTAGACAAGCTTTAAA
AGATTATTTAAAGCCTCATTTAAATAATCTTTCCTCTGATAGTCAAAATCGCTTTGAAACTAATCCCTTGAGAATTTTAG
ATTCAAAAAATGCTCAAGACAAACAAATTGTTGCTGACGCTCCCTCAATTCTAGATTTTCTCGACGAAGATTCTCAAATT
CATTTCGAACAGCTTAAAAAACTTCTTAAACAATTAAAAATTCCTTATCAAGTTAATCCCCTTCTCGTCCGAGGATTAGA
TTACTATAATAAAACTGTTTTTGAAGTAGTTGTGGGGGAACTGGGAGCTCAAAATAGTATTGGTGGAGGTGGAAGATATG
ATGGTCTTCTTCGAGAATTAGGTGGTCCTGACCTTCCGTCCATTGGCTTTGGAACCGGGATTGAACGTATCATTCAAACG
ATGATTAATCAAGGCATTCCCCTCCCTTCTCCTTGTCACCCCAGTTTATTTTTAATTCCAATGGGTGATGAAGCTAAACA
ACTTTGCTTTACTCTAACACATGAACTTAGACAACAAGGTATCCCGACTCAAATGGATTTCAGCGGAAAAAAGTTAGGAA
AAGTTCTACAATATGCTGATCAAATTGAATCTACCTATGTCGTTGTGATTGGAGAAAATGAACTCCAAACCCAAGAGATT
GAACTTAAAGAATTGGCAAGTGGAAAAAAATACAAATTAACCATTGATGAATTAGCTCCTACATTAAAAAATTGA

Upstream 100 bases:

>100_bases
AATCCTAGCGTTACGTTAGTTATTTTTCACCATTGCACTTTTTATATTTTTCCAAGTATCCTATTTTTTTCTTATTTTAC
GTTTGACTAAAAGGGCATTC

Downstream 100 bases:

>100_bases
TCTTCATGGACAATTTTATTTGAAGTTGTGGAAAGAATTATCTATTCCTTTTAAACGGAAAAATAAACGCAATTTTTCGT
TGAAAAGCTACATAAGTCTC

Product: histidine-tRNA ligase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MKFSIPPGVFDIIPTNTQEIWKSSYLWDFVERTIRKTVRDYGYQEIRTPLFERTELFQRSVGETSDIISKEMYTFEDKGG
RSMSLRPEGTAPVMRSFIENQLHTIAPLQKLFYIAPMFRYERAQAGRYRQHHQFGAEAIGNSSPEQDAELIDLLYTLYKR
LGLQNLSLNINSIGTISCRHTFRQALKDYLKPHLNNLSSDSQNRFETNPLRILDSKNAQDKQIVADAPSILDFLDEDSQI
HFEQLKKLLKQLKIPYQVNPLLVRGLDYYNKTVFEVVVGELGAQNSIGGGGRYDGLLRELGGPDLPSIGFGTGIERIIQT
MINQGIPLPSPCHPSLFLIPMGDEAKQLCFTLTHELRQQGIPTQMDFSGKKLGKVLQYADQIESTYVVVIGENELQTQEI
ELKELASGKKYKLTIDELAPTLKN

Sequences:

>Translated_424_residues
MKFSIPPGVFDIIPTNTQEIWKSSYLWDFVERTIRKTVRDYGYQEIRTPLFERTELFQRSVGETSDIISKEMYTFEDKGG
RSMSLRPEGTAPVMRSFIENQLHTIAPLQKLFYIAPMFRYERAQAGRYRQHHQFGAEAIGNSSPEQDAELIDLLYTLYKR
LGLQNLSLNINSIGTISCRHTFRQALKDYLKPHLNNLSSDSQNRFETNPLRILDSKNAQDKQIVADAPSILDFLDEDSQI
HFEQLKKLLKQLKIPYQVNPLLVRGLDYYNKTVFEVVVGELGAQNSIGGGGRYDGLLRELGGPDLPSIGFGTGIERIIQT
MINQGIPLPSPCHPSLFLIPMGDEAKQLCFTLTHELRQQGIPTQMDFSGKKLGKVLQYADQIESTYVVVIGENELQTQEI
ELKELASGKKYKLTIDELAPTLKN
>Mature_424_residues
MKFSIPPGVFDIIPTNTQEIWKSSYLWDFVERTIRKTVRDYGYQEIRTPLFERTELFQRSVGETSDIISKEMYTFEDKGG
RSMSLRPEGTAPVMRSFIENQLHTIAPLQKLFYIAPMFRYERAQAGRYRQHHQFGAEAIGNSSPEQDAELIDLLYTLYKR
LGLQNLSLNINSIGTISCRHTFRQALKDYLKPHLNNLSSDSQNRFETNPLRILDSKNAQDKQIVADAPSILDFLDEDSQI
HFEQLKKLLKQLKIPYQVNPLLVRGLDYYNKTVFEVVVGELGAQNSIGGGGRYDGLLRELGGPDLPSIGFGTGIERIIQT
MINQGIPLPSPCHPSLFLIPMGDEAKQLCFTLTHELRQQGIPTQMDFSGKKLGKVLQYADQIESTYVVVIGENELQTQEI
ELKELASGKKYKLTIDELAPTLKN

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=472, Percent_Identity=24.7881355932203, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1788861, Length=421, Percent_Identity=40.855106888361, Blast_Score=331, Evalue=7e-92,
Organism=Caenorhabditis elegans, GI71993693, Length=451, Percent_Identity=23.9467849223947, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71993686, Length=451, Percent_Identity=23.9467849223947, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_PARUW (Q6ME90)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007384.1
- ProteinModelPortal:   Q6ME90
- SMR:   Q6ME90
- STRING:   Q6ME90
- GeneID:   2780710
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0385
- NMPDR:   fig|264201.1.peg.385
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q6ME90
- ProtClustDB:   CLSK2762161
- BioCyc:   CPRO264201:PC0385-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 48242; Mature: 48242

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSIPPGVFDIIPTNTQEIWKSSYLWDFVERTIRKTVRDYGYQEIRTPLFERTELFQRS
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VGETSDIISKEMYTFEDKGGRSMSLRPEGTAPVMRSFIENQLHTIAPLQKLFYIAPMFRY
CCCHHHHHHHHHHHEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERAQAGRYRQHHQFGAEAIGNSSPEQDAELIDLLYTLYKRLGLQNLSLNINSIGTISCRH
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEHHHHH
TFRQALKDYLKPHLNNLSSDSQNRFETNPLRILDSKNAQDKQIVADAPSILDFLDEDSQI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHCCHHHHHHHCCCCCC
HFEQLKKLLKQLKIPYQVNPLLVRGLDYYNKTVFEVVVGELGAQNSIGGGGRYDGLLREL
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHC
GGPDLPSIGFGTGIERIIQTMINQGIPLPSPCHPSLFLIPMGDEAKQLCFTLTHELRQQG
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCC
IPTQMDFSGKKLGKVLQYADQIESTYVVVIGENELQTQEIELKELASGKKYKLTIDELAP
CCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEHHHHCH
TLKN
HHCC
>Mature Secondary Structure
MKFSIPPGVFDIIPTNTQEIWKSSYLWDFVERTIRKTVRDYGYQEIRTPLFERTELFQRS
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VGETSDIISKEMYTFEDKGGRSMSLRPEGTAPVMRSFIENQLHTIAPLQKLFYIAPMFRY
CCCHHHHHHHHHHHEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERAQAGRYRQHHQFGAEAIGNSSPEQDAELIDLLYTLYKRLGLQNLSLNINSIGTISCRH
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEHHHHH
TFRQALKDYLKPHLNNLSSDSQNRFETNPLRILDSKNAQDKQIVADAPSILDFLDEDSQI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHCCHHHHHHHCCCCCC
HFEQLKKLLKQLKIPYQVNPLLVRGLDYYNKTVFEVVVGELGAQNSIGGGGRYDGLLREL
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHC
GGPDLPSIGFGTGIERIIQTMINQGIPLPSPCHPSLFLIPMGDEAKQLCFTLTHELRQQG
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCC
IPTQMDFSGKKLGKVLQYADQIESTYVVVIGENELQTQEIELKELASGKKYKLTIDELAP
CCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEHHHHCH
TLKN
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA