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The map label for this gene is gyrB [H]

Identifier: 46445984

GI number: 46445984

Start: 476407

End: 478227

Strand: Direct

Name: gyrB [H]

Synonym: pc0350

Alternate gene names: 46445984

Gene position: 476407-478227 (Clockwise)

Preceding gene: 46445982

Following gene: 46445985

Centisome position: 19.73

GC content: 34.38

Gene sequence:

>1821_bases
ATGGCAAAAAACTATGATGAAAGTACAGTCAAAACGTTAGATGCGCTAGCTCACATCCGCTTGCGCTCTGGCATGTATAT
TGGTCGGTTAGGTGATGGAACTAACCCCGACGACGGAATTTATATTATGCTTAAAGAAGTCGTTGATAATAGCGTCGACG
AATTTATTATGAAGCACGGAAAAAAAATCATTATTGAGTTAGATGAAGATCTCAGTCGCGTCTCTGTTCGAGACTTTGGA
AGAGGAATTCCACTTGGCAAAGTAGTAGAATGTGTCAGCCAGATTAATACGGGTGCTAAATATAATGACGATGTTTTTCA
GTTTTCTGTAGGGCTTAATGGAGTGGGAACTAAAGCCGTTAATGCCCTTTCTAGTCATTTTGTTGTTAAAAGTTATCGAG
ATGGAGAATTTGTTGAAGCACATTTTTCTCAAGGATTGTTGAAACAAGAGAAGAAAGGAAAGACAAAGGAAGAAAATGGC
ACCTATGTGGAATTTATTCCTGATCCAGAAATTTTCAAAAAATATCGTTTTCAAAAAGAATATATTTTAAAACGTATTTG
GCATTATGCCTATTTGAATACGGGTCTAATTCTTCAATTTAATGGGGAAGAGATTCAATCTAAATATGGATTGCTAGACC
TTTTAAATGCGGAAGTCACAGAAGATAGGTTATATGAGCCTATTCATTATCGTGGTAAATTTTTAGAATTGGCTTTTTTA
CACACCCACAGTTATGGAGAAAGTTATTTTTCATTTGTCAATGGACAATATACGTCTGATGGAGGAACTCATCTTTCAGC
TTTTCGAGAAGGCATTTTAAAGGGAGTAAATGAGTTTACTAAGAAAAATTTCCAAGGAGTAGATGTACGAGAAGGAATTG
TGGGAACCATACTTGTAAAAGTGAAAGATCCAATCTTTGAATCGCAGACTAAAAATAAGCTAGGTAATAACGAATTACGC
GCTCCTATTGTCCAAGAAGTTAAAGAAGCCGTTGTAAATTTATTGCATAAACACCCCGATGTTGCGAATCGCTTAGTTGA
ACGAATTGTTTTTAATGAGAAGCTCCGCAAAGAACTGGCTTCCGTTAAAAAAGAAGCAAAGGAAAAGCAAAAGAAAATTT
CGTTCAAAATTCCCAAATTGAGAGATTCAAAGTATCATTATCAAGATCGATCGGTACATAGTGAAAACACAATGATTTTT
CTAACAGAAGGGGATTCGGCTAGTGCTTCGGTTGTGGCTTCTCGAGATCCATTAATACAGGCAGTTTATTCTTTAAGAGG
TAAACCTTTAAATGTATTTGGCATGAAGCTCGATCAACTCTATAAAAATGAAGAAATGTTCAATTTAATGAATGCTCTGA
ATATTGAAGATGAGATTGAAAAACTTCGTTATAATAAAGTCATTTTGGCAACAGATGCAGATGTCGACGGAATGCATATT
CGTAACCTAATGATAACTTTCTTTTTAACCTATTTTGAAGGACTTGTTTTAAACGGGCACTTATATATTTTAGAAACTCC
TTTATTTAAAGTACGTAATAAAGTGCAAACAATTTATTGCTATAATGAAGAAGAAAAAAATAAAGCGGTTGTTAAATTAA
AAAAACAAGTAGAAGTTACTCGTTTTAAAGGTCTCGGAGAAATTTCCCCCTCAGAATTTAAGCAATTTATAGGAAAGGAT
ATTCGCTTAATCCCTGTTACAATTCATTCTTTTTCTGATATTAAATCTACTTTGCAGTTTTATATGGGGAAAAATACCCC
AGAGCGGAAACAATTTATCATGCAAAACCTTATTAACGAAGATGAAATAGCTCCTGTATAG

Upstream 100 bases:

>100_bases
TTATAAATTTGGCAACCCTTAAAATTAATTGCTAAAAAACTAGATTTTCAATTGTTGAATCGTCTAAATTTTTTGTTCAC
AAACTAAAAGATGATTATAT

Downstream 100 bases:

>100_bases
CAAGCGAATTTTTAAAAAATATGTAAATTAAGCATGAGATGAACTCTTTGATCAAGTTATCAAGATATTAGGAATAGCGC
ATGGAAGATATTAAGCAATT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MAKNYDESTVKTLDALAHIRLRSGMYIGRLGDGTNPDDGIYIMLKEVVDNSVDEFIMKHGKKIIIELDEDLSRVSVRDFG
RGIPLGKVVECVSQINTGAKYNDDVFQFSVGLNGVGTKAVNALSSHFVVKSYRDGEFVEAHFSQGLLKQEKKGKTKEENG
TYVEFIPDPEIFKKYRFQKEYILKRIWHYAYLNTGLILQFNGEEIQSKYGLLDLLNAEVTEDRLYEPIHYRGKFLELAFL
HTHSYGESYFSFVNGQYTSDGGTHLSAFREGILKGVNEFTKKNFQGVDVREGIVGTILVKVKDPIFESQTKNKLGNNELR
APIVQEVKEAVVNLLHKHPDVANRLVERIVFNEKLRKELASVKKEAKEKQKKISFKIPKLRDSKYHYQDRSVHSENTMIF
LTEGDSASASVVASRDPLIQAVYSLRGKPLNVFGMKLDQLYKNEEMFNLMNALNIEDEIEKLRYNKVILATDADVDGMHI
RNLMITFFLTYFEGLVLNGHLYILETPLFKVRNKVQTIYCYNEEEKNKAVVKLKKQVEVTRFKGLGEISPSEFKQFIGKD
IRLIPVTIHSFSDIKSTLQFYMGKNTPERKQFIMQNLINEDEIAPV

Sequences:

>Translated_606_residues
MAKNYDESTVKTLDALAHIRLRSGMYIGRLGDGTNPDDGIYIMLKEVVDNSVDEFIMKHGKKIIIELDEDLSRVSVRDFG
RGIPLGKVVECVSQINTGAKYNDDVFQFSVGLNGVGTKAVNALSSHFVVKSYRDGEFVEAHFSQGLLKQEKKGKTKEENG
TYVEFIPDPEIFKKYRFQKEYILKRIWHYAYLNTGLILQFNGEEIQSKYGLLDLLNAEVTEDRLYEPIHYRGKFLELAFL
HTHSYGESYFSFVNGQYTSDGGTHLSAFREGILKGVNEFTKKNFQGVDVREGIVGTILVKVKDPIFESQTKNKLGNNELR
APIVQEVKEAVVNLLHKHPDVANRLVERIVFNEKLRKELASVKKEAKEKQKKISFKIPKLRDSKYHYQDRSVHSENTMIF
LTEGDSASASVVASRDPLIQAVYSLRGKPLNVFGMKLDQLYKNEEMFNLMNALNIEDEIEKLRYNKVILATDADVDGMHI
RNLMITFFLTYFEGLVLNGHLYILETPLFKVRNKVQTIYCYNEEEKNKAVVKLKKQVEVTRFKGLGEISPSEFKQFIGKD
IRLIPVTIHSFSDIKSTLQFYMGKNTPERKQFIMQNLINEDEIAPV
>Mature_605_residues
AKNYDESTVKTLDALAHIRLRSGMYIGRLGDGTNPDDGIYIMLKEVVDNSVDEFIMKHGKKIIIELDEDLSRVSVRDFGR
GIPLGKVVECVSQINTGAKYNDDVFQFSVGLNGVGTKAVNALSSHFVVKSYRDGEFVEAHFSQGLLKQEKKGKTKEENGT
YVEFIPDPEIFKKYRFQKEYILKRIWHYAYLNTGLILQFNGEEIQSKYGLLDLLNAEVTEDRLYEPIHYRGKFLELAFLH
THSYGESYFSFVNGQYTSDGGTHLSAFREGILKGVNEFTKKNFQGVDVREGIVGTILVKVKDPIFESQTKNKLGNNELRA
PIVQEVKEAVVNLLHKHPDVANRLVERIVFNEKLRKELASVKKEAKEKQKKISFKIPKLRDSKYHYQDRSVHSENTMIFL
TEGDSASASVVASRDPLIQAVYSLRGKPLNVFGMKLDQLYKNEEMFNLMNALNIEDEIEKLRYNKVILATDADVDGMHIR
NLMITFFLTYFEGLVLNGHLYILETPLFKVRNKVQTIYCYNEEEKNKAVVKLKKQVEVTRFKGLGEISPSEFKQFIGKDI
RLIPVTIHSFSDIKSTLQFYMGKNTPERKQFIMQNLINEDEIAPV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=608, Percent_Identity=26.8092105263158, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI19913406, Length=608, Percent_Identity=27.1381578947368, Blast_Score=124, Evalue=4e-28,
Organism=Escherichia coli, GI48994957, Length=568, Percent_Identity=33.2746478873239, Blast_Score=262, Evalue=5e-71,
Organism=Escherichia coli, GI1789408, Length=643, Percent_Identity=30.0155520995334, Blast_Score=244, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI212645845, Length=589, Percent_Identity=24.7877758913413, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17535065, Length=571, Percent_Identity=24.3432574430823, Blast_Score=108, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324241, Length=363, Percent_Identity=30.3030303030303, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17136538, Length=563, Percent_Identity=27.1758436944938, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 69504; Mature: 69373

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNYDESTVKTLDALAHIRLRSGMYIGRLGDGTNPDDGIYIMLKEVVDNSVDEFIMKHG
CCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEHHHHCCCHHHHHHHCC
KKIIIELDEDLSRVSVRDFGRGIPLGKVVECVSQINTGAKYNDDVFQFSVGLNGVGTKAV
CEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHH
NALSSHFVVKSYRDGEFVEAHFSQGLLKQEKKGKTKEENGTYVEFIPDPEIFKKYRFQKE
HHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHH
YILKRIWHYAYLNTGLILQFNGEEIQSKYGLLDLLNAEVTEDRLYEPIHYRGKFLELAFL
HHHHHHHHHHHHCCCEEEEECCHHHHHHHCHHHHHCCHHCHHHHCCCHHHCCCEEEEEEE
HTHSYGESYFSFVNGQYTSDGGTHLSAFREGILKGVNEFTKKNFQGVDVREGIVGTILVK
ECCCCHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCEEEEEEE
VKDPIFESQTKNKLGNNELRAPIVQEVKEAVVNLLHKHPDVANRLVERIVFNEKLRKELA
ECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
SVKKEAKEKQKKISFKIPKLRDSKYHYQDRSVHSENTMIFLTEGDSASASVVASRDPLIQ
HHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCHHHH
AVYSLRGKPLNVFGMKLDQLYKNEEMFNLMNALNIEDEIEKLRYNKVILATDADVDGMHI
HHHHHCCCCEEEEHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCEEEEEECCCCCCHHH
RNLMITFFLTYFEGLVLNGHLYILETPLFKVRNKVQTIYCYNEEEKNKAVVKLKKQVEVT
HHHHHHHHHHHHHHEEECCEEEEEECCHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHH
RFKGLGEISPSEFKQFIGKDIRLIPVTIHSFSDIKSTLQFYMGKNTPERKQFIMQNLINE
HHCCCCCCCHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
DEIAPV
CCCCCC
>Mature Secondary Structure 
AKNYDESTVKTLDALAHIRLRSGMYIGRLGDGTNPDDGIYIMLKEVVDNSVDEFIMKHG
CCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEHHHHCCCHHHHHHHCC
KKIIIELDEDLSRVSVRDFGRGIPLGKVVECVSQINTGAKYNDDVFQFSVGLNGVGTKAV
CEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHH
NALSSHFVVKSYRDGEFVEAHFSQGLLKQEKKGKTKEENGTYVEFIPDPEIFKKYRFQKE
HHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHH
YILKRIWHYAYLNTGLILQFNGEEIQSKYGLLDLLNAEVTEDRLYEPIHYRGKFLELAFL
HHHHHHHHHHHHCCCEEEEECCHHHHHHHCHHHHHCCHHCHHHHCCCHHHCCCEEEEEEE
HTHSYGESYFSFVNGQYTSDGGTHLSAFREGILKGVNEFTKKNFQGVDVREGIVGTILVK
ECCCCHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCEEEEEEE
VKDPIFESQTKNKLGNNELRAPIVQEVKEAVVNLLHKHPDVANRLVERIVFNEKLRKELA
ECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
SVKKEAKEKQKKISFKIPKLRDSKYHYQDRSVHSENTMIFLTEGDSASASVVASRDPLIQ
HHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCHHHH
AVYSLRGKPLNVFGMKLDQLYKNEEMFNLMNALNIEDEIEKLRYNKVILATDADVDGMHI
HHHHHCCCCEEEEHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCEEEEEECCCCCCHHH
RNLMITFFLTYFEGLVLNGHLYILETPLFKVRNKVQTIYCYNEEEKNKAVVKLKKQVEVT
HHHHHHHHHHHHHHEEECCEEEEEECCHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHH
RFKGLGEISPSEFKQFIGKDIRLIPVTIHSFSDIKSTLQFYMGKNTPERKQFIMQNLINE
HHCCCCCCCHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
DEIAPV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685; 7812434 [H]