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The map label for this gene is ksgA

Identifier: 161611309

GI number: 161611309

Start: 528658

End: 529512

Strand: Reverse

Name: ksgA

Synonym: pc0395

Alternate gene names: 161611309

Gene position: 529512-528658 (Counterclockwise)

Preceding gene: 46446030

Following gene: 46446027

Centisome position: 21.93

GC content: 37.08

Gene sequence:

>855_bases
ATGCCTATTTATAAACCCAGCGAATTACGCCTTTTTTTAAATCAACTTGGAATTTTCCCCAAAAAAGGACTTTCTCAAAA
TTTTTTGATTGATGGAAATATCATTCGTAAAATCGTTAGAGCCTCGGACGTACAACCAGGAAATCTTGTCTTAGAAATTG
GCCCAGGGCCAGGCTCATTAACACAAGCTATGCTAGAAGTAGAAGCCCATGTAGTGGCTGTTGAAAAGGACTTTGTACTA
GCCCGCGAATTAAAAAGATTTCAAACGCCATCAAAGCAACTTGAAATATTTTGTGAAGATATTCTAATGTTCTCAGTTGA
AGAAGAACTTCAAAGTCGATTAAGAGATGATCAGAAAGCAAAAGTGATTGCTAATCTCCCCTATCATTTGACAACTCCAA
TTCTCGCAGAAATGGTTGTTCGACGAAAACTTTTTTCTTCGTTAACTGTCATGGTACAAGAAGAAGTCGCTAGGCGTATG
ACAGCTTTACCTGGCCAATCTGATTATAGCTCTTTTACAATCTTTCTGAATTTTTATTCTAAGCCCCGCTATGGTTTTAC
GGTCAGCCGAAATTGCTTTTACCCTGCCCCAAAAGTTGATTCTGCTATCGTTGTTTTAGAATTAAAAGAGCCTCCTCCTA
ATATTGATGCACAAGTCTTTTTTAAAATCACTCGTACAGCCTTTGAACAGCGTCGTAAAATGCTTCGTGCCTCACTCAAA
TCTCTTTTTGACCCATCTAAAATATCAAACGCTTTAGAAATAATTGGGCAAAATCCTCAAGCTAGACCGGAAGTTTTATC
TTTAGAAGATTTTATTAAACTATATCACGAACTATATAGCTCTGAAAGGCATTAA

Upstream 100 bases:

>100_bases
ACCTCAAAAAATTACGAGAACATTTTTACGTCAGAGATAAAGACTTAGAAGCAATGGTTCCTGCTGACTATCAACCTTTT
GTTTTAAAATAAGCAAATTT

Downstream 100 bases:

>100_bases
TGCCATTCAGCATCGATAAACTCAAAATTTTCTTTTTTTAATAATCTCATTCATCATACTTTGCATTTAAAATAAATAAG
GACATTTTTTAACACGAGAG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MPIYKPSELRLFLNQLGIFPKKGLSQNFLIDGNIIRKIVRASDVQPGNLVLEIGPGPGSLTQAMLEVEAHVVAVEKDFVL
ARELKRFQTPSKQLEIFCEDILMFSVEEELQSRLRDDQKAKVIANLPYHLTTPILAEMVVRRKLFSSLTVMVQEEVARRM
TALPGQSDYSSFTIFLNFYSKPRYGFTVSRNCFYPAPKVDSAIVVLELKEPPPNIDAQVFFKITRTAFEQRRKMLRASLK
SLFDPSKISNALEIIGQNPQARPEVLSLEDFIKLYHELYSSERH

Sequences:

>Translated_284_residues
MPIYKPSELRLFLNQLGIFPKKGLSQNFLIDGNIIRKIVRASDVQPGNLVLEIGPGPGSLTQAMLEVEAHVVAVEKDFVL
ARELKRFQTPSKQLEIFCEDILMFSVEEELQSRLRDDQKAKVIANLPYHLTTPILAEMVVRRKLFSSLTVMVQEEVARRM
TALPGQSDYSSFTIFLNFYSKPRYGFTVSRNCFYPAPKVDSAIVVLELKEPPPNIDAQVFFKITRTAFEQRRKMLRASLK
SLFDPSKISNALEIIGQNPQARPEVLSLEDFIKLYHELYSSERH
>Mature_283_residues
PIYKPSELRLFLNQLGIFPKKGLSQNFLIDGNIIRKIVRASDVQPGNLVLEIGPGPGSLTQAMLEVEAHVVAVEKDFVLA
RELKRFQTPSKQLEIFCEDILMFSVEEELQSRLRDDQKAKVIANLPYHLTTPILAEMVVRRKLFSSLTVMVQEEVARRMT
ALPGQSDYSSFTIFLNFYSKPRYGFTVSRNCFYPAPKVDSAIVVLELKEPPPNIDAQVFFKITRTAFEQRRKMLRASLKS
LFDPSKISNALEIIGQNPQARPEVLSLEDFIKLYHELYSSERH

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=228, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI156415992, Length=274, Percent_Identity=30.6569343065693, Blast_Score=109, Evalue=2e-24,
Organism=Escherichia coli, GI1786236, Length=264, Percent_Identity=31.0606060606061, Blast_Score=122, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25141369, Length=272, Percent_Identity=30.5147058823529, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25146882, Length=237, Percent_Identity=31.2236286919831, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324989, Length=226, Percent_Identity=30.9734513274336, Blast_Score=102, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21358017, Length=250, Percent_Identity=32.4, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21357273, Length=279, Percent_Identity=29.7491039426523, Blast_Score=115, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_PARUW (Q6ME80)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007394.2
- ProteinModelPortal:   Q6ME80
- SMR:   Q6ME80
- STRING:   Q6ME80
- GeneID:   2780585
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0395
- NMPDR:   fig|264201.1.peg.395
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- PhylomeDB:   Q6ME80
- ProtClustDB:   PRK00274
- BioCyc:   CPRO264201:PC0395-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 32479; Mature: 32348

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 27-27 BINDING 29-29 BINDING 54-54 BINDING 75-75 BINDING 100-100 BINDING 125-125

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIYKPSELRLFLNQLGIFPKKGLSQNFLIDGNIIRKIVRASDVQPGNLVLEIGPGPGSL
CCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHCCCCCCCEEEEECCCCCHH
TQAMLEVEAHVVAVEKDFVLARELKRFQTPSKQLEIFCEDILMFSVEEELQSRLRDDQKA
HHHHHHHHHHEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KVIANLPYHLTTPILAEMVVRRKLFSSLTVMVQEEVARRMTALPGQSDYSSFTIFLNFYS
HEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECC
KPRYGFTVSRNCFYPAPKVDSAIVVLELKEPPPNIDAQVFFKITRTAFEQRRKMLRASLK
CCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLFDPSKISNALEIIGQNPQARPEVLSLEDFIKLYHELYSSERH
HHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PIYKPSELRLFLNQLGIFPKKGLSQNFLIDGNIIRKIVRASDVQPGNLVLEIGPGPGSL
CCCCCHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHCCCCCCCEEEEECCCCCHH
TQAMLEVEAHVVAVEKDFVLARELKRFQTPSKQLEIFCEDILMFSVEEELQSRLRDDQKA
HHHHHHHHHHEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KVIANLPYHLTTPILAEMVVRRKLFSSLTVMVQEEVARRMTALPGQSDYSSFTIFLNFYS
HEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECC
KPRYGFTVSRNCFYPAPKVDSAIVVLELKEPPPNIDAQVFFKITRTAFEQRRKMLRASLK
CCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLFDPSKISNALEIIGQNPQARPEVLSLEDFIKLYHELYSSERH
HHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA