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The map label for this gene is rlmL [H]

Identifier: 45658661

GI number: 45658661

Start: 3435735

End: 3436682

Strand: Direct

Name: rlmL [H]

Synonym: LIC12831

Alternate gene names: 45658661

Gene position: 3435735-3436682 (Clockwise)

Preceding gene: 45658660

Following gene: 45658662

Centisome position: 80.33

GC content: 38.71

Gene sequence:

>948_bases
ATGAAAACTGCAAAAAAAATTGAATCATCAAAAACAAATTCGCAAAAAAAATCTACTTCTGTAGAAAGATTTTCAGAAAC
AAACGCAAAAGTGATTGAAACGGGTTCTTATAAATTAATTGACTCCGGAAATTTTCGAAAGTTAGAACAAATCGGGCCTT
ATACGATCATCCGCCCTTCTCCCGTCTCCGCTTGGCCGGCCACAAAACCAAATCTATGGAACAATGTATATGGAGAATAC
GTACGTTCAGATAAAGGAGGAGGCTCCTGGAATTGGCATAAAAAAACAGAAGAAGAATTCTATATTCGAATTTTAGAATA
TTCTATTAAAATAAAATTTACTCCGTTCGGTCATCTTGGAATTTTTGCCGAGCAATTAGAAAACTGGAAAAAAATCCGTA
AACTTTCTTCTCAACTTAAAAAAGAAGACGAAGTTTTGAACCTTTTCGCTTATTCGGGAATTTCTACCTTGGCAGTGTTA
GACGGAGGGGCATCCGTTTGTCATCTAGATTCTTCCAAAGGGATGGTGGACTGGGCCCGAGATAACGCAAACGCCTCCGG
TCTTGCACACAAAAAAGTCCGTTGGATGGTAGAAGACGTATTGAAATTTTTAAAACGGGAAATCAAACGAGGAAAAGACT
ATCGAGGTTTTATCCTAGACCCTCCTACGTTTGGAAGAGGTGCAAGTGGAGAAGTATTTAAGATAGAAAAAGACCTTCCA
GAACTTATGGATCTTCTGATGGAGCTCTCCGGAAATAAACCAGATTTTATAGTACTTACCTGCCACTCTACTGGTTTTAG
CCCTCTGGCCCTACAAAGAATTTTAGACGGAAGAATCAACAACAAAGGAAGTTTTCATATAGGTGAACTTTCCATTCCAG
AAACAAGCGGAAGACTTTACCCCGCAGGCTCTAATTGTATTTATGTATCGGAGAAACTTTCGCTTTGA

Upstream 100 bases:

>100_bases
AAAGGATGGAAGTTTGAACGTTTTACGAGTTTAAAACCGGAGGTCGATCATCCGATCAGAAAGAATTTTCCGGAAGGAAA
TTACTTTAAGGTGCATATCT

Downstream 100 bases:

>100_bases
ACGACATTCAATTTTTGGAAATTACCAGTTTTTCAAACGAAAAGTTGAAGACAATTTCCAATCTCAAAGAAAAAAAACAC
AGAGAAACTTCGGGCCTTTT

Product: SAM dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKTAKKIESSKTNSQKKSTSVERFSETNAKVIETGSYKLIDSGNFRKLEQIGPYTIIRPSPVSAWPATKPNLWNNVYGEY
VRSDKGGGSWNWHKKTEEEFYIRILEYSIKIKFTPFGHLGIFAEQLENWKKIRKLSSQLKKEDEVLNLFAYSGISTLAVL
DGGASVCHLDSSKGMVDWARDNANASGLAHKKVRWMVEDVLKFLKREIKRGKDYRGFILDPPTFGRGASGEVFKIEKDLP
ELMDLLMELSGNKPDFIVLTCHSTGFSPLALQRILDGRINNKGSFHIGELSIPETSGRLYPAGSNCIYVSEKLSL

Sequences:

>Translated_315_residues
MKTAKKIESSKTNSQKKSTSVERFSETNAKVIETGSYKLIDSGNFRKLEQIGPYTIIRPSPVSAWPATKPNLWNNVYGEY
VRSDKGGGSWNWHKKTEEEFYIRILEYSIKIKFTPFGHLGIFAEQLENWKKIRKLSSQLKKEDEVLNLFAYSGISTLAVL
DGGASVCHLDSSKGMVDWARDNANASGLAHKKVRWMVEDVLKFLKREIKRGKDYRGFILDPPTFGRGASGEVFKIEKDLP
ELMDLLMELSGNKPDFIVLTCHSTGFSPLALQRILDGRINNKGSFHIGELSIPETSGRLYPAGSNCIYVSEKLSL
>Mature_315_residues
MKTAKKIESSKTNSQKKSTSVERFSETNAKVIETGSYKLIDSGNFRKLEQIGPYTIIRPSPVSAWPATKPNLWNNVYGEY
VRSDKGGGSWNWHKKTEEEFYIRILEYSIKIKFTPFGHLGIFAEQLENWKKIRKLSSQLKKEDEVLNLFAYSGISTLAVL
DGGASVCHLDSSKGMVDWARDNANASGLAHKKVRWMVEDVLKFLKREIKRGKDYRGFILDPPTFGRGASGEVFKIEKDLP
ELMDLLMELSGNKPDFIVLTCHSTGFSPLALQRILDGRINNKGSFHIGELSIPETSGRLYPAGSNCIYVSEKLSL

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=166, Percent_Identity=27.1084337349398, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 35478; Mature: 35478

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAKKIESSKTNSQKKSTSVERFSETNAKVIETGSYKLIDSGNFRKLEQIGPYTIIRPS
CCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCEEEEECCCCHHHHHCCCEEEECCC
PVSAWPATKPNLWNNVYGEYVRSDKGGGSWNWHKKTEEEFYIRILEYSIKIKFTPFGHLG
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEEEEEEECCCCHHH
IFAEQLENWKKIRKLSSQLKKEDEVLNLFAYSGISTLAVLDGGASVCHLDSSKGMVDWAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCEEEEC
DNANASGLAHKKVRWMVEDVLKFLKREIKRGKDYRGFILDPPTFGRGASGEVFKIEKDLP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEHHHHH
ELMDLLMELSGNKPDFIVLTCHSTGFSPLALQRILDGRINNKGSFHIGELSIPETSGRLY
HHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEE
PAGSNCIYVSEKLSL
ECCCCEEEEECCCCC
>Mature Secondary Structure
MKTAKKIESSKTNSQKKSTSVERFSETNAKVIETGSYKLIDSGNFRKLEQIGPYTIIRPS
CCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCEEEEECCCCHHHHHCCCEEEECCC
PVSAWPATKPNLWNNVYGEYVRSDKGGGSWNWHKKTEEEFYIRILEYSIKIKFTPFGHLG
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEEEEEEECCCCHHH
IFAEQLENWKKIRKLSSQLKKEDEVLNLFAYSGISTLAVLDGGASVCHLDSSKGMVDWAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCEEEEC
DNANASGLAHKKVRWMVEDVLKFLKREIKRGKDYRGFILDPPTFGRGASGEVFKIEKDLP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEHHHHH
ELMDLLMELSGNKPDFIVLTCHSTGFSPLALQRILDGRINNKGSFHIGELSIPETSGRLY
HHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEE
PAGSNCIYVSEKLSL
ECCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA