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The map label for this gene is rlmI [H]

Identifier: 45658660

GI number: 45658660

Start: 3434138

End: 3435757

Strand: Direct

Name: rlmI [H]

Synonym: LIC12830

Alternate gene names: 45658660

Gene position: 3434138-3435757 (Clockwise)

Preceding gene: 45658659

Following gene: 45658661

Centisome position: 80.29

GC content: 32.35

Gene sequence:

>1620_bases
TTGAACCGGAAGAGCGAAATTTCCATTCGACGCGGACATCCCTGGATCTTTGGCGGCAATCTTTCTTCTGCGACTTCTCA
TTTTGTTAACGGGCAATGGTTGAATTTAGTATCAACAACTAACGTTCCTTTGGCGACCGGAATTTATTCTACAACAGGCT
TAATCCGAATACGGGTCATTCAATTTTTACCTTCTTTTTCAAAAGAAAAAATGAGAGAAAACTTAATTTATGCTCTCAAA
AAAAGAAACGAACTTAGAAAAATCACAAATGCATACAGAATTTTACACGGAGAAAACGATTTTTTTCCGGGAATTACAAT
CGATCGATTAAATACTACCTGGGTGGTTCGTATTTATTCCTCTTCTTTACTTGTATACGGAAGATGGTTGGTCTGGAATT
TATTTGATATATGCAAAAATTCTAAATTAGGGGAACCTCAACCAAAACGGATTTTATTTGATCCCCCCGAAAAAACCGGC
GAAGACAAAATCGGTTTTAAAAAAAAAGATCCTGCCGATCATTCTCAAACGTTTACCCCCTCCAAATTGGATAAATCTAA
TTTTAACCAAAACTTGACTTCCAAAAAATCAAATTTTAAAAAAATCTCTTCCTCAGAAACATTAGAAAAAAAAATTTTAG
ATAATTTTAATTTAGAAAATGATCTTTCGACTTTTTCACAAAACACTAACCTATCGGATAACGATTCCAAAGAAATTTTA
AAGTATCCTAAGCAAAACTCGAACTTATCAAATTCAGATCTTTTCAAAACATCACAAGAGAATATATTCTATAATTCTAT
TTTAAAAAAACAGAATTTAGAATCTACAATCAACTTGATCCATGAAAGATTATGGAGAGGAAATCGGCAATCAACCAGAA
ACAGAAGTAATTTTACAAAAGAAAAAACAAAACATTCCATACAAAAGGAAATCTATCATTCAGATAAATATAAAATTAGA
GAAACGATCCGACTGTATGAAGTAAATTTTCCGATCGAACTTCCAGGTCAAAAAGGAGGAATTTTTTTAGATCTCAGAAA
TTTACGTAGATTTCTTCTCGAAAAAAAAGAACTGAGCAAAAATAAAAATTGTCTTCATCTGTTTTCTCATACTGGACTCA
CTTCTATTTGTATGGAAATAGCAGGAGCCTCTTCCGTTATTTCGGTAGACGGTTCCAAAGAAGCCTTGGATTCGTTTCAA
AGAGTTTTGAGTCTAAATAAAAATAAAGGAAATTGTAAACATAGATTTTTACGGAAAAATATTTTTAAAGAATTAGAAGA
AATTCTACAAAACAAATCATTTGGATTAATCGTAATCGATCCTCCCAATCTCACTCCGGATGAAAAATCTAAATCAAACG
CTCTTAAATCCTATTCCTATCTTTTTGCAAATAGTCTTTCTTCTTTGGAAGAATCGGGAACGATCATACTTTGTTCTTGT
TCAGGTAGAATTAAAACCCAAGAATTAGAATCTCTTGCAAAGAACATTCTTCAATCGAAAGGATGGAAGTTTGAACGTTT
TACGAGTTTAAAACCGGAGGTCGATCATCCGATCAGAAAGAATTTTCCGGAAGGAAATTACTTTAAGGTGCATATCTATG
AAAACTGCAAAAAAAATTGA

Upstream 100 bases:

>100_bases
CAAGACAAATCTACACCGGTTCTACAGAAACTTCCTACAAAGAAGCCAAGAAAAAAGCCTGAACTCTCATTCTACAAACT
TCCGGTTTCATAGATACCAA

Downstream 100 bases:

>100_bases
ATCATCAAAAACAAATTCGCAAAAAAAATCTACTTCTGTAGAAAGATTTTCAGAAACAAACGCAAAAGTGATTGAAACGG
GTTCTTATAAATTAATTGAC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MNRKSEISIRRGHPWIFGGNLSSATSHFVNGQWLNLVSTTNVPLATGIYSTTGLIRIRVIQFLPSFSKEKMRENLIYALK
KRNELRKITNAYRILHGENDFFPGITIDRLNTTWVVRIYSSSLLVYGRWLVWNLFDICKNSKLGEPQPKRILFDPPEKTG
EDKIGFKKKDPADHSQTFTPSKLDKSNFNQNLTSKKSNFKKISSSETLEKKILDNFNLENDLSTFSQNTNLSDNDSKEIL
KYPKQNSNLSNSDLFKTSQENIFYNSILKKQNLESTINLIHERLWRGNRQSTRNRSNFTKEKTKHSIQKEIYHSDKYKIR
ETIRLYEVNFPIELPGQKGGIFLDLRNLRRFLLEKKELSKNKNCLHLFSHTGLTSICMEIAGASSVISVDGSKEALDSFQ
RVLSLNKNKGNCKHRFLRKNIFKELEEILQNKSFGLIVIDPPNLTPDEKSKSNALKSYSYLFANSLSSLEESGTIILCSC
SGRIKTQELESLAKNILQSKGWKFERFTSLKPEVDHPIRKNFPEGNYFKVHIYENCKKN

Sequences:

>Translated_539_residues
MNRKSEISIRRGHPWIFGGNLSSATSHFVNGQWLNLVSTTNVPLATGIYSTTGLIRIRVIQFLPSFSKEKMRENLIYALK
KRNELRKITNAYRILHGENDFFPGITIDRLNTTWVVRIYSSSLLVYGRWLVWNLFDICKNSKLGEPQPKRILFDPPEKTG
EDKIGFKKKDPADHSQTFTPSKLDKSNFNQNLTSKKSNFKKISSSETLEKKILDNFNLENDLSTFSQNTNLSDNDSKEIL
KYPKQNSNLSNSDLFKTSQENIFYNSILKKQNLESTINLIHERLWRGNRQSTRNRSNFTKEKTKHSIQKEIYHSDKYKIR
ETIRLYEVNFPIELPGQKGGIFLDLRNLRRFLLEKKELSKNKNCLHLFSHTGLTSICMEIAGASSVISVDGSKEALDSFQ
RVLSLNKNKGNCKHRFLRKNIFKELEEILQNKSFGLIVIDPPNLTPDEKSKSNALKSYSYLFANSLSSLEESGTIILCSC
SGRIKTQELESLAKNILQSKGWKFERFTSLKPEVDHPIRKNFPEGNYFKVHIYENCKKN
>Mature_539_residues
MNRKSEISIRRGHPWIFGGNLSSATSHFVNGQWLNLVSTTNVPLATGIYSTTGLIRIRVIQFLPSFSKEKMRENLIYALK
KRNELRKITNAYRILHGENDFFPGITIDRLNTTWVVRIYSSSLLVYGRWLVWNLFDICKNSKLGEPQPKRILFDPPEKTG
EDKIGFKKKDPADHSQTFTPSKLDKSNFNQNLTSKKSNFKKISSSETLEKKILDNFNLENDLSTFSQNTNLSDNDSKEIL
KYPKQNSNLSNSDLFKTSQENIFYNSILKKQNLESTINLIHERLWRGNRQSTRNRSNFTKEKTKHSIQKEIYHSDKYKIR
ETIRLYEVNFPIELPGQKGGIFLDLRNLRRFLLEKKELSKNKNCLHLFSHTGLTSICMEIAGASSVISVDGSKEALDSFQ
RVLSLNKNKGNCKHRFLRKNIFKELEEILQNKSFGLIVIDPPNLTPDEKSKSNALKSYSYLFANSLSSLEESGTIILCSC
SGRIKTQELESLAKNILQSKGWKFERFTSLKPEVDHPIRKNFPEGNYFKVHIYENCKKN

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=195, Percent_Identity=30.2564102564103, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1787181, Length=162, Percent_Identity=32.7160493827161, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 62290; Mature: 62290

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKSEISIRRGHPWIFGGNLSSATSHFVNGQWLNLVSTTNVPLATGIYSTTGLIRIRVI
CCCCCCEEEECCCCEEECCCCCHHHHHHCCCCEEEEEEECCCCEECCHHHHHHHHHHHHH
QFLPSFSKEKMRENLIYALKKRNELRKITNAYRILHGENDFFPGITIDRLNTTWVVRIYS
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCCCEEEEEEEC
SSLLVYGRWLVWNLFDICKNSKLGEPQPKRILFDPPEKTGEDKIGFKKKDPADHSQTFTP
CCEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
SKLDKSNFNQNLTSKKSNFKKISSSETLEKKILDNFNLENDLSTFSQNTNLSDNDSKEIL
CCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHH
KYPKQNSNLSNSDLFKTSQENIFYNSILKKQNLESTINLIHERLWRGNRQSTRNRSNFTK
HCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHH
EKTKHSIQKEIYHSDKYKIRETIRLYEVNFPIELPGQKGGIFLDLRNLRRFLLEKKELSK
HHHHHHHHHHHHHCCHHHHHHEEEEEEECCCEECCCCCCCEEEEHHHHHHHHHHHHHHCC
NKNCLHLFSHTGLTSICMEIAGASSVISVDGSKEALDSFQRVLSLNKNKGNCKHRFLRKN
CCCEEEEHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IFKELEEILQNKSFGLIVIDPPNLTPDEKSKSNALKSYSYLFANSLSSLEESGTIILCSC
HHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
SGRIKTQELESLAKNILQSKGWKFERFTSLKPEVDHPIRKNFPEGNYFKVHIYENCKKN
CCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MNRKSEISIRRGHPWIFGGNLSSATSHFVNGQWLNLVSTTNVPLATGIYSTTGLIRIRVI
CCCCCCEEEECCCCEEECCCCCHHHHHHCCCCEEEEEEECCCCEECCHHHHHHHHHHHHH
QFLPSFSKEKMRENLIYALKKRNELRKITNAYRILHGENDFFPGITIDRLNTTWVVRIYS
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCCCEEEEEEEC
SSLLVYGRWLVWNLFDICKNSKLGEPQPKRILFDPPEKTGEDKIGFKKKDPADHSQTFTP
CCEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
SKLDKSNFNQNLTSKKSNFKKISSSETLEKKILDNFNLENDLSTFSQNTNLSDNDSKEIL
CCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHH
KYPKQNSNLSNSDLFKTSQENIFYNSILKKQNLESTINLIHERLWRGNRQSTRNRSNFTK
HCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHH
EKTKHSIQKEIYHSDKYKIRETIRLYEVNFPIELPGQKGGIFLDLRNLRRFLLEKKELSK
HHHHHHHHHHHHHCCHHHHHHEEEEEEECCCEECCCCCCCEEEEHHHHHHHHHHHHHHCC
NKNCLHLFSHTGLTSICMEIAGASSVISVDGSKEALDSFQRVLSLNKNKGNCKHRFLRKN
CCCEEEEHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IFKELEEILQNKSFGLIVIDPPNLTPDEKSKSNALKSYSYLFANSLSSLEESGTIILCSC
HHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
SGRIKTQELESLAKNILQSKGWKFERFTSLKPEVDHPIRKNFPEGNYFKVHIYENCKKN
CCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA