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The map label for this gene is yjfH

Identifier: 45658662

GI number: 45658662

Start: 3436679

End: 3437503

Strand: Direct

Name: yjfH

Synonym: LIC12832

Alternate gene names: 45658662

Gene position: 3436679-3437503 (Clockwise)

Preceding gene: 45658661

Following gene: 45658663

Centisome position: 80.35

GC content: 35.39

Gene sequence:

>825_bases
TTGAACGACATTCAATTTTTGGAAATTACCAGTTTTTCAAACGAAAAGTTGAAGACAATTTCCAATCTCAAAGAAAAAAA
ACACAGAGAAACTTCGGGCCTTTTTTTTATAGAAGGGTATCGTGAAATTTTAAGAGCACAAAAATCTGGAAAGATAAAGT
TTCAAAACATACTTTTTTCTCCGGAATGTTTTTTAGGAGAAAACGAATTTTCACTTATTCGTTCGATCGGAACCAAAAAC
ATCAAAGTTCCTAAAAAGATTTTTGAAAAAGTTTCTTATAGAGACAGACCTGACGGACTGATCGCAACCGCATTTTTTTT
TGAAACAGGACTAGATACCTTTCAAAACAAATCTAATATATTCAAAAATTTGAAACCGATTTTAGTGATCGAAGGCGTGG
AAAAACCTGGAAACCTAGGAACAATTCTGAGAACCGCGGAAGGAGCAGGGTTTCATACTGTACTTGTAGCCGATCCTAAA
CTGGATCTTTTTAATCCGAACGTAATCCGTGCTTCTACAGGAGCTCTTTTTACTTTAGAAGTTTATCTTGGAGAAATAAA
ACTGATTTATGAAATTTTAAAAAAGAACCAGTATAGAACATTAGCCGTCACACCAGAAGCTACAAAACATTACTATAATG
CGGATTTAAAGGGCAAAATAGCGCTCGTCTTCGGCTCGGAACAATACGGTCTTTCTACTTATGCAAGAAACAATTCCGAT
GAATACGTTTCACTTCCTATGTTCGGAGAATCGGATTCACTCAACTTAGCAATGTCTGCTGGAATCGTTATGTACGAAGT
CATTAGACAAGTTTCCGTTTTATGA

Upstream 100 bases:

>100_bases
AAGGAAGTTTTCATATAGGTGAACTTTCCATTCCAGAAACAAGCGGAAGACTTTACCCCGCAGGCTCTAATTGTATTTAT
GTATCGGAGAAACTTTCGCT

Downstream 100 bases:

>100_bases
AAATCACTTATTACGTTAGCGGTCATGGATTTGGTCATATCAGCCGGTCCATGGAAATCATTCTTTATTTACTTAGAAGT
TTTCCAGATTTAACAATCGA

Product: rRNA methylase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNDIQFLEITSFSNEKLKTISNLKEKKHRETSGLFFIEGYREILRAQKSGKIKFQNILFSPECFLGENEFSLIRSIGTKN
IKVPKKIFEKVSYRDRPDGLIATAFFFETGLDTFQNKSNIFKNLKPILVIEGVEKPGNLGTILRTAEGAGFHTVLVADPK
LDLFNPNVIRASTGALFTLEVYLGEIKLIYEILKKNQYRTLAVTPEATKHYYNADLKGKIALVFGSEQYGLSTYARNNSD
EYVSLPMFGESDSLNLAMSAGIVMYEVIRQVSVL

Sequences:

>Translated_274_residues
MNDIQFLEITSFSNEKLKTISNLKEKKHRETSGLFFIEGYREILRAQKSGKIKFQNILFSPECFLGENEFSLIRSIGTKN
IKVPKKIFEKVSYRDRPDGLIATAFFFETGLDTFQNKSNIFKNLKPILVIEGVEKPGNLGTILRTAEGAGFHTVLVADPK
LDLFNPNVIRASTGALFTLEVYLGEIKLIYEILKKNQYRTLAVTPEATKHYYNADLKGKIALVFGSEQYGLSTYARNNSD
EYVSLPMFGESDSLNLAMSAGIVMYEVIRQVSVL
>Mature_274_residues
MNDIQFLEITSFSNEKLKTISNLKEKKHRETSGLFFIEGYREILRAQKSGKIKFQNILFSPECFLGENEFSLIRSIGTKN
IKVPKKIFEKVSYRDRPDGLIATAFFFETGLDTFQNKSNIFKNLKPILVIEGVEKPGNLGTILRTAEGAGFHTVLVADPK
LDLFNPNVIRASTGALFTLEVYLGEIKLIYEILKKNQYRTLAVTPEATKHYYNADLKGKIALVFGSEQYGLSTYARNNSD
EYVSLPMFGESDSLNLAMSAGIVMYEVIRQVSVL

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=147, Percent_Identity=30.6122448979592, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30937; Mature: 30937

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIQFLEITSFSNEKLKTISNLKEKKHRETSGLFFIEGYREILRAQKSGKIKFQNILFS
CCCCEEEEEECCCCHHHHHHHHHHHHHCCHHCCEEEEHHHHHHHHHHHCCCEEEEEEEEC
PECFLGENEFSLIRSIGTKNIKVPKKIFEKVSYRDRPDGLIATAFFFETGLDTFQNKSNI
CCCEECCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEHHHHHHCHHHHHHHHHH
FKNLKPILVIEGVEKPGNLGTILRTAEGAGFHTVLVADPKLDLFNPNVIRASTGALFTLE
HHCCCCEEEEECCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCEEEECCCCEEEEE
VYLGEIKLIYEILKKNQYRTLAVTPEATKHYYNADLKGKIALVFGSEQYGLSTYARNNSD
EHHHHHHHHHHHHHCCCCEEEEECCHHHHHEECCCCCEEEEEEECCCCCCCEEEECCCCC
EYVSLPMFGESDSLNLAMSAGIVMYEVIRQVSVL
CEEEEEECCCCCCCEEEHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNDIQFLEITSFSNEKLKTISNLKEKKHRETSGLFFIEGYREILRAQKSGKIKFQNILFS
CCCCEEEEEECCCCHHHHHHHHHHHHHCCHHCCEEEEHHHHHHHHHHHCCCEEEEEEEEC
PECFLGENEFSLIRSIGTKNIKVPKKIFEKVSYRDRPDGLIATAFFFETGLDTFQNKSNI
CCCEECCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEHHHHHHCHHHHHHHHHH
FKNLKPILVIEGVEKPGNLGTILRTAEGAGFHTVLVADPKLDLFNPNVIRASTGALFTLE
HHCCCCEEEEECCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCEEEECCCCEEEEE
VYLGEIKLIYEILKKNQYRTLAVTPEATKHYYNADLKGKIALVFGSEQYGLSTYARNNSD
EHHHHHHHHHHHHHCCCCEEEEECCHHHHHEECCCCCEEEEEEECCCCCCCEEEECCCCC
EYVSLPMFGESDSLNLAMSAGIVMYEVIRQVSVL
CEEEEEECCCCCCCEEEHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]