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The map label for this gene is purH

Identifier: 45657525

GI number: 45657525

Start: 2020188

End: 2021723

Strand: Reverse

Name: purH

Synonym: LIC11655

Alternate gene names: 45657525

Gene position: 2021723-2020188 (Counterclockwise)

Preceding gene: 45657526

Following gene: 45657524

Centisome position: 47.27

GC content: 38.41

Gene sequence:

>1536_bases
ATGATACAAATCAAAAGAGCTCTCATCTCCGTCAGCGATAAATCCGGTTTAGTGGAATTTGCAAAGTTTCTAAACCAAAA
CGGAGTGGAAATCATTTCCACAGGTGGAACTTTAAAACTTCTAAAAGATAACGGAATTGCAGCCATAGCTATCGACGATT
ACACCGGTTTTCCGGAAATTTTAGATGGAAGAGTCAAAACCCTTCATCCTAAAGTTCACGGAGGTCTATTAGGTGTAATT
TCCAATCCTGCTCACAAACAAAAAATGGAAGAATTAAAAATTCCTAAAATAGATTTAGTCGTAGTCAACTTATATCCTTT
TTTAAAGACCGTTTCTAAACCAGAAGTTCAATTAGAAGAAGCAATCGAAAACATAGATATTGGTGGGCCTTCTATGATTC
GAAGCGCCGCTAAAAATTATAAACATACTCTAGTGCTCACTGATCCAAATGATTACAAGGAAATTCAAAATTTAATCTCC
TCCAGTGGAATTTCAGAAGAAATTTCAGCGAGTTATATGAGAAAAGCATTTTCTCACACTGCGATGTATGATGCCGCGAT
TTCTTCCTGGTTTTACAAACAATCAGGAGAAGTTTTTCCAGATGTTCTCAATCTTTCCTTTATCAAAAAACAAAAACTGA
GATACGGCGAAAATCCGCACCAAGCCGCCTCATTTTATGAACCACTTTTTACAAAGAGCGATTTTTCACCTTTACAAGGG
AAAGAGTTGTCGTTTAACAATATGTTAGATTTTGACGCTGCCTTTCATATTTCTAGCCTTCTTCCCGAAAATACAGTATG
TATCATCAAACATCTCAATCCTTGTGGAATTGCTTATGCAGACAATCCATTAGAAGCATTTCAACTTGCAAGAAGAACCG
ATCCTATTTCCGCATTTGGTGGAGTAATAGGAATTAAAGGACAAGTAAACGGAGAATTGGCGACTTCGATCACTGAAAAC
TTTGTAGAAGGAGTGATCGCTCAAAAATTCACTCAAGAAGCTCTGGAAGTATTTTCTAAAAAACCAAATATTCGTTTAAT
AGAAATCCAAGATTTCAAAGAAGCCCTTGATGAGTTGGATTTAAGACCGATCCATCACGGTTTGCTTATACAAGATAGAG
ATTATACAACAATCACTGAAAAAGATCTAAAAGTAGTCACTAAAAAACAACCTTCTCCCGATGATATTCGTGGTTTGATG
TTTGCTTGGTCTTGTGTTCGTTTTATCAAATCCAACGCAATCGTTTATACGGAAGAAAATGCAACTCTAGGAATCGGAGC
GGGACAAATGTCCAGAGTCGACTCAGTGCAGTTAGGCGCCAACAAAGCGCTGAACGTAGGTCTTTCCGTAGTAGGTTCTT
ATGTTGCAAGCGACGCATTCTTCCCATTCCGAGATGGAATCGACACACTTGCAAAAGCAGGAGCAAAAGCGATCATTCAA
CCAGGTGGTTCTGTCCGAGACGAGGAAGTAATTCAAGCCGCAGACGAACACGGACTGATTATGGTTTTTACTGGAATGAG
GCATTTTAGACACTGA

Upstream 100 bases:

>100_bases
GATTCTCAAAGAGGAACATAAAATACTACCACTCGCGGTTCAATATTTTTGCGAGGATAGGCTTACGATTCAAAATAGAA
AGGTAAAGATTGGATAAATA

Downstream 100 bases:

>100_bases
TTATGGAATTTCTACTTTATCTAATCTTTTTTGGAATTGTTTCCATCTTACTCGTCTTAGCAAGTTATTACTTTAAACTT
CTCTTTTTATCCGGAAGAGA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MIQIKRALISVSDKSGLVEFAKFLNQNGVEIISTGGTLKLLKDNGIAAIAIDDYTGFPEILDGRVKTLHPKVHGGLLGVI
SNPAHKQKMEELKIPKIDLVVVNLYPFLKTVSKPEVQLEEAIENIDIGGPSMIRSAAKNYKHTLVLTDPNDYKEIQNLIS
SSGISEEISASYMRKAFSHTAMYDAAISSWFYKQSGEVFPDVLNLSFIKKQKLRYGENPHQAASFYEPLFTKSDFSPLQG
KELSFNNMLDFDAAFHISSLLPENTVCIIKHLNPCGIAYADNPLEAFQLARRTDPISAFGGVIGIKGQVNGELATSITEN
FVEGVIAQKFTQEALEVFSKKPNIRLIEIQDFKEALDELDLRPIHHGLLIQDRDYTTITEKDLKVVTKKQPSPDDIRGLM
FAWSCVRFIKSNAIVYTEENATLGIGAGQMSRVDSVQLGANKALNVGLSVVGSYVASDAFFPFRDGIDTLAKAGAKAIIQ
PGGSVRDEEVIQAADEHGLIMVFTGMRHFRH

Sequences:

>Translated_511_residues
MIQIKRALISVSDKSGLVEFAKFLNQNGVEIISTGGTLKLLKDNGIAAIAIDDYTGFPEILDGRVKTLHPKVHGGLLGVI
SNPAHKQKMEELKIPKIDLVVVNLYPFLKTVSKPEVQLEEAIENIDIGGPSMIRSAAKNYKHTLVLTDPNDYKEIQNLIS
SSGISEEISASYMRKAFSHTAMYDAAISSWFYKQSGEVFPDVLNLSFIKKQKLRYGENPHQAASFYEPLFTKSDFSPLQG
KELSFNNMLDFDAAFHISSLLPENTVCIIKHLNPCGIAYADNPLEAFQLARRTDPISAFGGVIGIKGQVNGELATSITEN
FVEGVIAQKFTQEALEVFSKKPNIRLIEIQDFKEALDELDLRPIHHGLLIQDRDYTTITEKDLKVVTKKQPSPDDIRGLM
FAWSCVRFIKSNAIVYTEENATLGIGAGQMSRVDSVQLGANKALNVGLSVVGSYVASDAFFPFRDGIDTLAKAGAKAIIQ
PGGSVRDEEVIQAADEHGLIMVFTGMRHFRH
>Mature_511_residues
MIQIKRALISVSDKSGLVEFAKFLNQNGVEIISTGGTLKLLKDNGIAAIAIDDYTGFPEILDGRVKTLHPKVHGGLLGVI
SNPAHKQKMEELKIPKIDLVVVNLYPFLKTVSKPEVQLEEAIENIDIGGPSMIRSAAKNYKHTLVLTDPNDYKEIQNLIS
SSGISEEISASYMRKAFSHTAMYDAAISSWFYKQSGEVFPDVLNLSFIKKQKLRYGENPHQAASFYEPLFTKSDFSPLQG
KELSFNNMLDFDAAFHISSLLPENTVCIIKHLNPCGIAYADNPLEAFQLARRTDPISAFGGVIGIKGQVNGELATSITEN
FVEGVIAQKFTQEALEVFSKKPNIRLIEIQDFKEALDELDLRPIHHGLLIQDRDYTTITEKDLKVVTKKQPSPDDIRGLM
FAWSCVRFIKSNAIVYTEENATLGIGAGQMSRVDSVQLGANKALNVGLSVVGSYVASDAFFPFRDGIDTLAKAGAKAIIQ
PGGSVRDEEVIQAADEHGLIMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=469, Percent_Identity=35.8208955223881, Blast_Score=250, Evalue=2e-66,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=48.5714285714286, Blast_Score=505, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI71985564, Length=601, Percent_Identity=30.4492512479201, Blast_Score=251, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI71985556, Length=61, Percent_Identity=45.9016393442623, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71985574, Length=342, Percent_Identity=23.9766081871345, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323768, Length=470, Percent_Identity=34.2553191489362, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6323056, Length=473, Percent_Identity=34.46088794926, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24649832, Length=483, Percent_Identity=33.5403726708075, Blast_Score=230, Evalue=2e-60,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56349; Mature: 56349

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQIKRALISVSDKSGLVEFAKFLNQNGVEIISTGGTLKLLKDNGIAAIAIDDYTGFPEI
CCEEEEHHHCCCCCCCHHHHHHHHCCCCCEEEECCCEEEEEECCCEEEEEECCCCCCHHH
LDGRVKTLHPKVHGGLLGVISNPAHKQKMEELKIPKIDLVVVNLYPFLKTVSKPEVQLEE
HCCCHHEECCHHCCCEEEECCCCHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCHHHHH
AIENIDIGGPSMIRSAAKNYKHTLVLTDPNDYKEIQNLISSSGISEEISASYMRKAFSHT
HHHHCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AMYDAAISSWFYKQSGEVFPDVLNLSFIKKQKLRYGENPHQAASFYEPLFTKSDFSPLQG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC
KELSFNNMLDFDAAFHISSLLPENTVCIIKHLNPCGIAYADNPLEAFQLARRTDPISAFG
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCHHHHHHHHHCCCCHHHHC
GVIGIKGQVNGELATSITENFVEGVIAQKFTQEALEVFSKKPNIRLIEIQDFKEALDELD
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCC
LRPIHHGLLIQDRDYTTITEKDLKVVTKKQPSPDDIRGLMFAWSCVRFIKSNAIVYTEEN
CCCCCCCEEEECCCCCEECCCHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECC
ATLGIGAGQMSRVDSVQLGANKALNVGLSVVGSYVASDAFFPFRDGIDTLAKAGAKAIIQ
CEEEECCCCHHCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHCCCCEEEC
PGGSVRDEEVIQAADEHGLIMVFTGMRHFRH
CCCCCCHHHHHHHHCCCCEEEEEECHHHHCC
>Mature Secondary Structure
MIQIKRALISVSDKSGLVEFAKFLNQNGVEIISTGGTLKLLKDNGIAAIAIDDYTGFPEI
CCEEEEHHHCCCCCCCHHHHHHHHCCCCCEEEECCCEEEEEECCCEEEEEECCCCCCHHH
LDGRVKTLHPKVHGGLLGVISNPAHKQKMEELKIPKIDLVVVNLYPFLKTVSKPEVQLEE
HCCCHHEECCHHCCCEEEECCCCHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCHHHHH
AIENIDIGGPSMIRSAAKNYKHTLVLTDPNDYKEIQNLISSSGISEEISASYMRKAFSHT
HHHHCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AMYDAAISSWFYKQSGEVFPDVLNLSFIKKQKLRYGENPHQAASFYEPLFTKSDFSPLQG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC
KELSFNNMLDFDAAFHISSLLPENTVCIIKHLNPCGIAYADNPLEAFQLARRTDPISAFG
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCHHHHHHHHHCCCCHHHHC
GVIGIKGQVNGELATSITENFVEGVIAQKFTQEALEVFSKKPNIRLIEIQDFKEALDELD
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCC
LRPIHHGLLIQDRDYTTITEKDLKVVTKKQPSPDDIRGLMFAWSCVRFIKSNAIVYTEEN
CCCCCCCEEEECCCCCEECCCHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECC
ATLGIGAGQMSRVDSVQLGANKALNVGLSVVGSYVASDAFFPFRDGIDTLAKAGAKAIIQ
CEEEECCCCHHCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHCCCCEEEC
PGGSVRDEEVIQAADEHGLIMVFTGMRHFRH
CCCCCCHHHHHHHHCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA