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The map label for this gene is purN

Identifier: 45657526

GI number: 45657526

Start: 2021727

End: 2022353

Strand: Reverse

Name: purN

Synonym: LIC11656

Alternate gene names: 45657526

Gene position: 2022353-2021727 (Counterclockwise)

Preceding gene: 45657530

Following gene: 45657525

Centisome position: 47.28

GC content: 38.12

Gene sequence:

>627_bases
TTGGCAACTCTGTTTACAAAACCTAAAAAAAAGATCGTATTTTTAGCTTCCGGAAGAGGCTCTAATCTCAGAGCAGTTTT
ACAAAATATCAAAGTAGGAAAAATTCGAGGAATCGCACAAATACTGATTTGCGATCATCCGGATGCCAAAGCTTTAGAAG
TTGCTCAGGAATTTGAACTCACCTCTCAAGTATTAAACTTCTCTTCTTTTTCGAATAAGTCGGAATATCATACAAAACTT
CTCCAACTTCTTTTAGAAATCAAACCGGACCTCATCGTAACCGCCGGTTATATGAGAATTCTTAAGAGCCCAGTCATTCA
AACGTTTTCCAATCGAATCATCAATATTCATCCATCTCTCTTACCCGCCTTTCCAGGATTAAACGCACAGAAACAAGCCC
TAGAATACGGAGTTAAAATTGCGGGTTGCACCGCTCATTTTGTAGACGAAGGTATAGACTCAGGTCCGATCATTTTACAA
GGAGTTGTGAAAATCGAAGAAGGAATGACGGAAAGAGATTTGACTTTAGAGATTCTCAAAGAGGAACATAAAATACTACC
ACTCGCGGTTCAATATTTTTGCGAGGATAGGCTTACGATTCAAAATAGAAAGGTAAAGATTGGATAA

Upstream 100 bases:

>100_bases
TTCATGGACTTTTGGTTAGACAAATTATCGCTTAAAGTTTTCCAAACTACATTTTTTTACAAATTCGAAAACGATTCGAT
TTTATACGGATAAAATTCTA

Downstream 100 bases:

>100_bases
ATAATGATACAAATCAAAAGAGCTCTCATCTCCGTCAGCGATAAATCCGGTTTAGTGGAATTTGCAAAGTTTCTAAACCA
AAACGGAGTGGAAATCATTT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MATLFTKPKKKIVFLASGRGSNLRAVLQNIKVGKIRGIAQILICDHPDAKALEVAQEFELTSQVLNFSSFSNKSEYHTKL
LQLLLEIKPDLIVTAGYMRILKSPVIQTFSNRIINIHPSLLPAFPGLNAQKQALEYGVKIAGCTAHFVDEGIDSGPIILQ
GVVKIEEGMTERDLTLEILKEEHKILPLAVQYFCEDRLTIQNRKVKIG

Sequences:

>Translated_208_residues
MATLFTKPKKKIVFLASGRGSNLRAVLQNIKVGKIRGIAQILICDHPDAKALEVAQEFELTSQVLNFSSFSNKSEYHTKL
LQLLLEIKPDLIVTAGYMRILKSPVIQTFSNRIINIHPSLLPAFPGLNAQKQALEYGVKIAGCTAHFVDEGIDSGPIILQ
GVVKIEEGMTERDLTLEILKEEHKILPLAVQYFCEDRLTIQNRKVKIG
>Mature_207_residues
ATLFTKPKKKIVFLASGRGSNLRAVLQNIKVGKIRGIAQILICDHPDAKALEVAQEFELTSQVLNFSSFSNKSEYHTKLL
QLLLEIKPDLIVTAGYMRILKSPVIQTFSNRIINIHPSLLPAFPGLNAQKQALEYGVKIAGCTAHFVDEGIDSGPIILQG
VVKIEEGMTERDLTLEILKEEHKILPLAVQYFCEDRLTIQNRKVKIG

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=187, Percent_Identity=38.5026737967914, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI209869995, Length=187, Percent_Identity=38.5026737967914, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI209869993, Length=187, Percent_Identity=38.5026737967914, Blast_Score=143, Evalue=1e-34,
Organism=Escherichia coli, GI1788846, Length=191, Percent_Identity=38.2198952879581, Blast_Score=154, Evalue=3e-39,
Organism=Escherichia coli, GI1787483, Length=166, Percent_Identity=32.5301204819277, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17567511, Length=184, Percent_Identity=35.3260869565217, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320616, Length=176, Percent_Identity=30.1136363636364, Blast_Score=68, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=38.6243386243386, Blast_Score=139, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23177; Mature: 23045

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLFTKPKKKIVFLASGRGSNLRAVLQNIKVGKIRGIAQILICDHPDAKALEVAQEFEL
CCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCHHCCEEEEEEECCCCHHHHHHHHHHHH
TSQVLNFSSFSNKSEYHTKLLQLLLEIKPDLIVTAGYMRILKSPVIQTFSNRIINIHPSL
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCEEEECCHH
LPAFPGLNAQKQALEYGVKIAGCTAHFVDEGIDSGPIILQGVVKIEEGMTERDLTLEILK
HCCCCCCCHHHHHHHHCCEEECHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
EEHKILPLAVQYFCEDRLTIQNRKVKIG
HCCCHHHHHHHHHHCCCCEEECCEEECC
>Mature Secondary Structure 
ATLFTKPKKKIVFLASGRGSNLRAVLQNIKVGKIRGIAQILICDHPDAKALEVAQEFEL
CCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCHHCCEEEEEEECCCCHHHHHHHHHHHH
TSQVLNFSSFSNKSEYHTKLLQLLLEIKPDLIVTAGYMRILKSPVIQTFSNRIINIHPSL
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCEEEECCHH
LPAFPGLNAQKQALEYGVKIAGCTAHFVDEGIDSGPIILQGVVKIEEGMTERDLTLEILK
HCCCCCCCHHHHHHHHCCEEECHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
EEHKILPLAVQYFCEDRLTIQNRKVKIG
HCCCHHHHHHHHHHCCCCEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]