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The map label for this gene is pleD [H]

Identifier: 45657011

GI number: 45657011

Start: 1391208

End: 1392149

Strand: Reverse

Name: pleD [H]

Synonym: LIC11127

Alternate gene names: 45657011

Gene position: 1392149-1391208 (Counterclockwise)

Preceding gene: 45657012

Following gene: 45657010

Centisome position: 32.55

GC content: 40.66

Gene sequence:

>942_bases
ATGCAGTACGATTATGAAAAATTTTTTCACAACTCTTTGGACATACTTGTTATTGCCAGAATGGACAATGGTCACGTAAT
CCACGTAAATCCTTCCTTTGAAAGGATTTTTGGTTGGTCGGAAAAACGTCTGTTAGGGTTAGGTTCTTACGAGTTTCTTC
ATCCGGAAGATCTTCCTTCTACGCAAGAAATAGCCGCCAAACTTGCTACTGGCGCTCCGATCGTATCTTTTGAAAATCGT
TATCGTTCGATTGACGGAAATTACAGAACCTTATCTTGGACTGCAGTACCGGAATATAAAAGTGGTATGGTTTACGCAAT
TGCAAGAGACATTACGGAAGCAATCGAATCCAACCGTAAAATCAGCGAACTCGCTACGGAACTCAAAGAAGCAAATAATA
TGTTACTAGAACAAGCGAGCACAGACCCTTTGACTAAATTAAAAAATAGAAGGGCATTCAACGCAGAACTCAATCATTTA
CTTCAAATTGCTCAAAAACAGTCAAATCCTATCTCTTTATTGATGATAGACGCGGACCATTTCAAAGATTACAATGATAA
ATTCGGACATCCGGCGGGAGATCAGGTTTTGATTGACCTAGCTTTTCTCTTAACTCATACCCTACGCCAACACGATGTGA
TCGCAAGATATGGAGGAGAAGAATTCATAGTCGCCATGCCCGATACTTCGGAAGTAGCTTCCTTGCAAATCGCGGAAAGA
TTGATTCACGTCGTAGGAGAATTCAACTGGGAAAAACGTCCAGTGACGATCAGCATAGGTGCAGCAACCCTCCCCGGCGA
AAAACAAAAAACTTCTCTTGAAGAAAACCGTCATTTTATAAATCTCATCGAATCCGCAGACAAAGCCTTGTATCATTCTA
AGATAAACGGAAGAAATCAAGTAAACCATAGTTCCAGGATCCTTCCTTGTAAAGAAGCGTAA

Upstream 100 bases:

>100_bases
CCCAAATATCTTGAAACGGTTTATGAGCACTTTTGAATGTAATTCAATTTTTCCAAAATATTTTTATCTTTCTATTTTTT
TTAAATGAATTGGCTAACAA

Downstream 100 bases:

>100_bases
TCCTATAGAAAAAACCTACGCGTCTGTTAATCGGCGAACCTATAATAAGAACGACTTTACTTATAAATCTTTTTTGGAAA
AACGTCACATGAGTTATCAA

Product: GGDEF family protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MQYDYEKFFHNSLDILVIARMDNGHVIHVNPSFERIFGWSEKRLLGLGSYEFLHPEDLPSTQEIAAKLATGAPIVSFENR
YRSIDGNYRTLSWTAVPEYKSGMVYAIARDITEAIESNRKISELATELKEANNMLLEQASTDPLTKLKNRRAFNAELNHL
LQIAQKQSNPISLLMIDADHFKDYNDKFGHPAGDQVLIDLAFLLTHTLRQHDVIARYGGEEFIVAMPDTSEVASLQIAER
LIHVVGEFNWEKRPVTISIGAATLPGEKQKTSLEENRHFINLIESADKALYHSKINGRNQVNHSSRILPCKEA

Sequences:

>Translated_313_residues
MQYDYEKFFHNSLDILVIARMDNGHVIHVNPSFERIFGWSEKRLLGLGSYEFLHPEDLPSTQEIAAKLATGAPIVSFENR
YRSIDGNYRTLSWTAVPEYKSGMVYAIARDITEAIESNRKISELATELKEANNMLLEQASTDPLTKLKNRRAFNAELNHL
LQIAQKQSNPISLLMIDADHFKDYNDKFGHPAGDQVLIDLAFLLTHTLRQHDVIARYGGEEFIVAMPDTSEVASLQIAER
LIHVVGEFNWEKRPVTISIGAATLPGEKQKTSLEENRHFINLIESADKALYHSKINGRNQVNHSSRILPCKEA
>Mature_313_residues
MQYDYEKFFHNSLDILVIARMDNGHVIHVNPSFERIFGWSEKRLLGLGSYEFLHPEDLPSTQEIAAKLATGAPIVSFENR
YRSIDGNYRTLSWTAVPEYKSGMVYAIARDITEAIESNRKISELATELKEANNMLLEQASTDPLTKLKNRRAFNAELNHL
LQIAQKQSNPISLLMIDADHFKDYNDKFGHPAGDQVLIDLAFLLTHTLRQHDVIARYGGEEFIVAMPDTSEVASLQIAER
LIHVVGEFNWEKRPVTISIGAATLPGEKQKTSLEENRHFINLIESADKALYHSKINGRNQVNHSSRILPCKEA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788381, Length=286, Percent_Identity=31.4685314685315, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI87081881, Length=307, Percent_Identity=30.9446254071661, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87082007, Length=182, Percent_Identity=35.7142857142857, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI145693134, Length=195, Percent_Identity=35.8974358974359, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=37.2781065088757, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786584, Length=179, Percent_Identity=32.9608938547486, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787802, Length=180, Percent_Identity=32.7777777777778, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787541, Length=178, Percent_Identity=31.4606741573034, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081977, Length=101, Percent_Identity=42.5742574257426, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=30.5882352941176, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788956, Length=148, Percent_Identity=34.4594594594595, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=30.1775147928994, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87081974, Length=101, Percent_Identity=31.6831683168317, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787056, Length=180, Percent_Identity=30, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35515; Mature: 35515

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50112 PAS ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYDYEKFFHNSLDILVIARMDNGHVIHVNPSFERIFGWSEKRLLGLGSYEFLHPEDLPS
CCCCHHHHHCCCCEEEEEEEECCCEEEEECCCHHHHHCCCCCCEEECCCCCCCCCCCCCC
TQEIAAKLATGAPIVSFENRYRSIDGNYRTLSWTAVPEYKSGMVYAIARDITEAIESNRK
HHHHHHHHHCCCCEEEECHHHHCCCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHCCCH
ISELATELKEANNMLLEQASTDPLTKLKNRRAFNAELNHLLQIAQKQSNPISLLMIDADH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHH
FKDYNDKFGHPAGDQVLIDLAFLLTHTLRQHDVIARYGGEEFIVAMPDTSEVASLQIAER
CCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHH
LIHVVGEFNWEKRPVTISIGAATLPGEKQKTSLEENRHFINLIESADKALYHSKINGRNQ
HHHHHHCCCCCCCCEEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VNHSSRILPCKEA
CCCCCCCCCCCCC
>Mature Secondary Structure
MQYDYEKFFHNSLDILVIARMDNGHVIHVNPSFERIFGWSEKRLLGLGSYEFLHPEDLPS
CCCCHHHHHCCCCEEEEEEEECCCEEEEECCCHHHHHCCCCCCEEECCCCCCCCCCCCCC
TQEIAAKLATGAPIVSFENRYRSIDGNYRTLSWTAVPEYKSGMVYAIARDITEAIESNRK
HHHHHHHHHCCCCEEEECHHHHCCCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHCCCH
ISELATELKEANNMLLEQASTDPLTKLKNRRAFNAELNHLLQIAQKQSNPISLLMIDADH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHH
FKDYNDKFGHPAGDQVLIDLAFLLTHTLRQHDVIARYGGEEFIVAMPDTSEVASLQIAER
CCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHH
LIHVVGEFNWEKRPVTISIGAATLPGEKQKTSLEENRHFINLIESADKALYHSKINGRNQ
HHHHHHCCCCCCCCEEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VNHSSRILPCKEA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA