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The map label for this gene is pleD [H]

Identifier: 45657012

GI number: 45657012

Start: 1392236

End: 1393162

Strand: Reverse

Name: pleD [H]

Synonym: LIC11128

Alternate gene names: 45657012

Gene position: 1393162-1392236 (Counterclockwise)

Preceding gene: 45657013

Following gene: 45657011

Centisome position: 32.57

GC content: 38.83

Gene sequence:

>927_bases
ATGACGAATTTTGAAAAAGAATACGATCTTGAAAAACTTGTAAACAACTCTTTGGATTTACTATCGATTCAGAGATTGGA
CGGGACTGTATTACAAGTAAACCCCGCCTTTGAACGTCTTCTGGGTTGGAGAGAGGAAGAACTGATTGGTAGAAATCCAT
TTCATTTACTCCACCCCGAAGATAGAGAAAGTACCTTTCAAGAGTTTAAAAAATTAAACCAAGGTTTACCTGTTTTTGCC
TTTCAAAATCGTTTTCTTTGTTCCGACGGAACTTATAAATATTTTTCTTGGACCGCCTCTCCAGATCTATCAGCAGGCTT
GATTTATGTAACCGGAAGGGATATCAGCGATCTAATCGAATCCAATCGTAAAATCAGCCTGTTAGCGGCGGAACTTAAAG
ACGCAAACGATAAGTTATTTGAACAGGCTTGTACCGATCCATTGACAAAATTGAAAAATAGAAGAGCCTTTAACGAAGAA
CTAAATTCTCTAATCCAACTGTCTCAAAAACAACTTAGCCCTCTTTCCTTGTTGATGATCGATGTGGATTTTTTCAAAGA
CTATAACGACAAATTTGGACATCCGGCCGGAGATAAAGTTCTGGCTATATTGGCGTCTCTTTTAGCAAAAACTCTTAGAA
AAGATGACGTGACCGCACGCTACGGCGGAGAAGAATTTATCGTAGCATTACCAAACACATCCGAAAAAGAAGCGATCGAA
ATCGCAGAAAGATTGGTTAAAGTAATTGAAGAATCGAGATGGGAAAAAAGATCCGTTACGATCAGCGTAGGAATCACCAC
TTCTCAAATAAATCCATCAAATAATGCTGACCATTCGATAGACATTATAGAAGAAGCGGATCGCGCGTTATACCACTCAA
AGGTCAACGGAAGAAACAGAGTTACCCATAGTTCCCAAATATCTTGA

Upstream 100 bases:

>100_bases
ATTGTTCCAAAGTAAGCGGTAGGGATAGAATCACACATTTTTCATGGGTCCAAAATAAAAAGAGCGGATCTATTTAAAAT
CAATATTATGGAATTGTAAA

Downstream 100 bases:

>100_bases
AACGGTTTATGAGCACTTTTGAATGTAATTCAATTTTTCCAAAATATTTTTATCTTTCTATTTTTTTTAAATGAATTGGC
TAACAAATGCAGTACGATTA

Product: GGDEF family protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTNFEKEYDLEKLVNNSLDLLSIQRLDGTVLQVNPAFERLLGWREEELIGRNPFHLLHPEDRESTFQEFKKLNQGLPVFA
FQNRFLCSDGTYKYFSWTASPDLSAGLIYVTGRDISDLIESNRKISLLAAELKDANDKLFEQACTDPLTKLKNRRAFNEE
LNSLIQLSQKQLSPLSLLMIDVDFFKDYNDKFGHPAGDKVLAILASLLAKTLRKDDVTARYGGEEFIVALPNTSEKEAIE
IAERLVKVIEESRWEKRSVTISVGITTSQINPSNNADHSIDIIEEADRALYHSKVNGRNRVTHSSQIS

Sequences:

>Translated_308_residues
MTNFEKEYDLEKLVNNSLDLLSIQRLDGTVLQVNPAFERLLGWREEELIGRNPFHLLHPEDRESTFQEFKKLNQGLPVFA
FQNRFLCSDGTYKYFSWTASPDLSAGLIYVTGRDISDLIESNRKISLLAAELKDANDKLFEQACTDPLTKLKNRRAFNEE
LNSLIQLSQKQLSPLSLLMIDVDFFKDYNDKFGHPAGDKVLAILASLLAKTLRKDDVTARYGGEEFIVALPNTSEKEAIE
IAERLVKVIEESRWEKRSVTISVGITTSQINPSNNADHSIDIIEEADRALYHSKVNGRNRVTHSSQIS
>Mature_307_residues
TNFEKEYDLEKLVNNSLDLLSIQRLDGTVLQVNPAFERLLGWREEELIGRNPFHLLHPEDRESTFQEFKKLNQGLPVFAF
QNRFLCSDGTYKYFSWTASPDLSAGLIYVTGRDISDLIESNRKISLLAAELKDANDKLFEQACTDPLTKLKNRRAFNEEL
NSLIQLSQKQLSPLSLLMIDVDFFKDYNDKFGHPAGDKVLAILASLLAKTLRKDDVTARYGGEEFIVALPNTSEKEAIEI
AERLVKVIEESRWEKRSVTISVGITTSQINPSNNADHSIDIIEEADRALYHSKVNGRNRVTHSSQIS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=41.9753086419753, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788381, Length=335, Percent_Identity=28.955223880597, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=37.4233128834356, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=34.7826086956522, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI145693134, Length=172, Percent_Identity=36.046511627907, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081881, Length=304, Percent_Identity=29.9342105263158, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=34.8101265822785, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=31.7919075144509, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=32.7272727272727, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787541, Length=316, Percent_Identity=26.2658227848101, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87081977, Length=173, Percent_Identity=32.3699421965318, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87081974, Length=223, Percent_Identity=26.9058295964126, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788956, Length=168, Percent_Identity=31.547619047619, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787056, Length=251, Percent_Identity=26.6932270916335, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35023; Mature: 34892

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50112 PAS ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNFEKEYDLEKLVNNSLDLLSIQRLDGTVLQVNPAFERLLGWREEELIGRNPFHLLHPE
CCCCCHHHHHHHHHCCCCCEEEEECCCCEEEEECHHHHHHHCCCHHHHCCCCCEEECCCC
DRESTFQEFKKLNQGLPVFAFQNRFLCSDGTYKYFSWTASPDLSAGLIYVTGRDISDLIE
CHHHHHHHHHHHHCCCCEEEECCEEEECCCCEEEEEEECCCCCCCCEEEEECCCHHHHHH
SNRKISLLAAELKDANDKLFEQACTDPLTKLKNRRAFNEELNSLIQLSQKQLSPLSLLMI
CCCEEEEEEHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DVDFFKDYNDKFGHPAGDKVLAILASLLAKTLRKDDVTARYGGEEFIVALPNTSEKEAIE
EHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCHHHHHH
IAERLVKVIEESRWEKRSVTISVGITTSQINPSNNADHSIDIIEEADRALYHSKVNGRNR
HHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VTHSSQIS
CCCCCCCC
>Mature Secondary Structure 
TNFEKEYDLEKLVNNSLDLLSIQRLDGTVLQVNPAFERLLGWREEELIGRNPFHLLHPE
CCCCHHHHHHHHHCCCCCEEEEECCCCEEEEECHHHHHHHCCCHHHHCCCCCEEECCCC
DRESTFQEFKKLNQGLPVFAFQNRFLCSDGTYKYFSWTASPDLSAGLIYVTGRDISDLIE
CHHHHHHHHHHHHCCCCEEEECCEEEECCCCEEEEEEECCCCCCCCEEEEECCCHHHHHH
SNRKISLLAAELKDANDKLFEQACTDPLTKLKNRRAFNEELNSLIQLSQKQLSPLSLLMI
CCCEEEEEEHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DVDFFKDYNDKFGHPAGDKVLAILASLLAKTLRKDDVTARYGGEEFIVALPNTSEKEAIE
EHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCHHHHHH
IAERLVKVIEESRWEKRSVTISVGITTSQINPSNNADHSIDIIEEADRALYHSKVNGRNR
HHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VTHSSQIS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA