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The map label for this gene is pckA

Identifier: 45656121

GI number: 45656121

Start: 245239

End: 246831

Strand: Direct

Name: pckA

Synonym: LIC10216

Alternate gene names: 45656121

Gene position: 245239-246831 (Clockwise)

Preceding gene: 45656120

Following gene: 45656126

Centisome position: 5.73

GC content: 38.67

Gene sequence:

>1593_bases
ATGCAGGCCCAGACTCAGGTAAAAGGTTTGAAGGAACTCGGACTCGAACCTTCTGAAATTTTCCATAACCTTTCCTATGA
TGAAATTTATGAACATGAGAAAAAAAACGGAGAAACTGTAGTTTCCAGCAATGGAACTATGATGGTAGATACTGGTATTT
TTACAGGTAGATCTCCGAAAGATAAATATTTCGTAGATGAGCCTTCCTCAAATGGAAACATCTGGTGGAGTCATATCAAT
TTCAAAGTTTCAGAAGCAATCTTTGACGAGCTTTATAAGAAATGTGTAAACTATCTGAGTCATAAAAAACTATACGTTTT
CGATGGTTACGCAGGTGCAAATCCTGAAACCAGAGTAAGTTTGCGAGTTGTTTCCGAAAAAGCTTGGCAACATCATTTTT
GTACGAATATGTTTCTTCGTCCTACAAAAGAAGAACTTGTCGGTCTTGATCCTGAATTTACGATTATCAATGCTTGTGGA
ATTAAGAATGAAAACTTCAAACAGCACGGAATGAATTCCGAAGTGTTTGTAATCTTCCATCTTGCTAAGAAGATTTGTAT
CATCGGCGGAACCGAATATGGCGGAGAGATGAAAAAAGGTATTTTCTCCGTTATGAATTACAAACTTCCTTTGCAGGGAA
TTCTCAGCATGCACTGTTCCGCGAATGTTGGTCAAGATGGTGATACGGCTCTTTTCTTTGGTCTTTCTGGAACTGGTAAA
ACAACTCTTTCTACAGACCCAAACCGCAAATTGATCGGAGACGATGAACACGGTTGGGATGATAACGGAATTTTTAATAT
CGAAGGTGGTTGTTATGCTAAGGTAATTAACTTAGATCCTAAAACTGAGCCGGACATTTACGAGGCCATTCGTAAAGACG
CCCTTCTTGAAAACGTAGTTTATGATCCTCAGACTAAGATTGTAGATTATTCTTCTGCTGCAAAAACGGAAAATACTCGA
GTTTCTTATCCGATTTTCCACATCAATAACATCCAAGTTCCTTCTAAAGGAGGACATCCGAAAACCATTATATTCTTAAC
TTATGATGCGTTTGGCGTTCTTCCTCCCGTTTCCAAATTGAGTATTGAACAGGCGATGTATCACTTTCTTTCCGGTTATA
CCGCGAAGGTTGCGGGCACGGAAAGAGGGATCAAAGAGCCAACCGCTACTTTTTCTGCATGTTTTGGAGCTGCGTTTATG
ACTCTTCATCCTACGAAATACGCTAAATTACTCGGTGAAAAAATGAAAAAGCATAACGTCAGGGCTTACTTGATGAACAC
TGGTCTTGTAGGTGGGTCTTACGGAGTTGGAAAACGTATGAATCTTCCTTCTACTCGTAAAATCATCGATGAGATTTTAA
ACGGGAATATAGAAAAGTCTGAGTTTGTGACACATCCAGTTTTTCAAGTAGCTTATCCTAAAACGATCAGCGGAGTTGAT
AGTGCTATTCTTGATCCAAGAGAAGCTTGGACAGATAAAGCTGCTTATGATCAAACTGCTAAAAAATTAGGGGAAATGTT
CATTAAGAATTTTAAACAATATGCGGAAGGATCAAAGGATTTCGATTTTACTGCATTTGGCCCGAAAATTTAA

Upstream 100 bases:

>100_bases
GTTTCCGAGAAAGGAAATCGTCAACCCCATTCCTCTTGAATCGATTGACTTTCCACTTTAGAGTAAAATAATTGAAACAG
AATTCCCAAGGAGCAATCAA

Downstream 100 bases:

>100_bases
TTTTGATCTTTGTAAGATCTACGGAAGCTAAAACTACTTTCCGTAGATCTTTTATAATGTTTGAATTTTTAAGAATAATT
TAAACTTACTATGAAGTCTT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MQAQTQVKGLKELGLEPSEIFHNLSYDEIYEHEKKNGETVVSSNGTMMVDTGIFTGRSPKDKYFVDEPSSNGNIWWSHIN
FKVSEAIFDELYKKCVNYLSHKKLYVFDGYAGANPETRVSLRVVSEKAWQHHFCTNMFLRPTKEELVGLDPEFTIINACG
IKNENFKQHGMNSEVFVIFHLAKKICIIGGTEYGGEMKKGIFSVMNYKLPLQGILSMHCSANVGQDGDTALFFGLSGTGK
TTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKVINLDPKTEPDIYEAIRKDALLENVVYDPQTKIVDYSSAAKTENTR
VSYPIFHINNIQVPSKGGHPKTIIFLTYDAFGVLPPVSKLSIEQAMYHFLSGYTAKVAGTERGIKEPTATFSACFGAAFM
TLHPTKYAKLLGEKMKKHNVRAYLMNTGLVGGSYGVGKRMNLPSTRKIIDEILNGNIEKSEFVTHPVFQVAYPKTISGVD
SAILDPREAWTDKAAYDQTAKKLGEMFIKNFKQYAEGSKDFDFTAFGPKI

Sequences:

>Translated_530_residues
MQAQTQVKGLKELGLEPSEIFHNLSYDEIYEHEKKNGETVVSSNGTMMVDTGIFTGRSPKDKYFVDEPSSNGNIWWSHIN
FKVSEAIFDELYKKCVNYLSHKKLYVFDGYAGANPETRVSLRVVSEKAWQHHFCTNMFLRPTKEELVGLDPEFTIINACG
IKNENFKQHGMNSEVFVIFHLAKKICIIGGTEYGGEMKKGIFSVMNYKLPLQGILSMHCSANVGQDGDTALFFGLSGTGK
TTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKVINLDPKTEPDIYEAIRKDALLENVVYDPQTKIVDYSSAAKTENTR
VSYPIFHINNIQVPSKGGHPKTIIFLTYDAFGVLPPVSKLSIEQAMYHFLSGYTAKVAGTERGIKEPTATFSACFGAAFM
TLHPTKYAKLLGEKMKKHNVRAYLMNTGLVGGSYGVGKRMNLPSTRKIIDEILNGNIEKSEFVTHPVFQVAYPKTISGVD
SAILDPREAWTDKAAYDQTAKKLGEMFIKNFKQYAEGSKDFDFTAFGPKI
>Mature_530_residues
MQAQTQVKGLKELGLEPSEIFHNLSYDEIYEHEKKNGETVVSSNGTMMVDTGIFTGRSPKDKYFVDEPSSNGNIWWSHIN
FKVSEAIFDELYKKCVNYLSHKKLYVFDGYAGANPETRVSLRVVSEKAWQHHFCTNMFLRPTKEELVGLDPEFTIINACG
IKNENFKQHGMNSEVFVIFHLAKKICIIGGTEYGGEMKKGIFSVMNYKLPLQGILSMHCSANVGQDGDTALFFGLSGTGK
TTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKVINLDPKTEPDIYEAIRKDALLENVVYDPQTKIVDYSSAAKTENTR
VSYPIFHINNIQVPSKGGHPKTIIFLTYDAFGVLPPVSKLSIEQAMYHFLSGYTAKVAGTERGIKEPTATFSACFGAAFM
TLHPTKYAKLLGEKMKKHNVRAYLMNTGLVGGSYGVGKRMNLPSTRKIIDEILNGNIEKSEFVTHPVFQVAYPKTISGVD
SAILDPREAWTDKAAYDQTAKKLGEMFIKNFKQYAEGSKDFDFTAFGPKI

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=524, Percent_Identity=53.2442748091603, Blast_Score=577, Evalue=1e-166,
Organism=Saccharomyces cerevisiae, GI6322950, Length=536, Percent_Identity=46.0820895522388, Blast_Score=478, Evalue=1e-135,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_LEPIC (Q72VT0)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000207.1
- ProteinModelPortal:   Q72VT0
- GeneID:   2772203
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10216
- HOGENOM:   HBG293387
- OMA:   MFIRPTE
- ProtClustDB:   PRK09344
- BioCyc:   LINT267671:LIC_10216-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 59175; Mature: 59175

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAQTQVKGLKELGLEPSEIFHNLSYDEIYEHEKKNGETVVSSNGTMMVDTGIFTGRSPK
CCCHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHCCCCCEEEECCCEEEEEECEECCCCCC
DKYFVDEPSSNGNIWWSHINFKVSEAIFDELYKKCVNYLSHKKLYVFDGYAGANPETRVS
CCEEEECCCCCCCEEEEEECEEHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEE
LRVVSEKAWQHHFCTNMFLRPTKEELVGLDPEFTIINACGIKNENFKQHGMNSEVFVIFH
EEEEHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCCEEEEEE
LAKKICIIGGTEYGGEMKKGIFSVMNYKLPLQGILSMHCSANVGQDGDTALFFGLSGTGK
EHHCEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCC
TTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKVINLDPKTEPDIYEAIRKDALLENVV
EEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
YDPQTKIVDYSSAAKTENTRVSYPIFHINNIQVPSKGGHPKTIIFLTYDAFGVLPPVSKL
CCCCCCEEECCCCCCCCCCEEEEEEEEECCEECCCCCCCCCEEEEEEECCCCCCCCHHHH
SIEQAMYHFLSGYTAKVAGTERGIKEPTATFSACFGAAFMTLHPTKYAKLLGEKMKKHNV
HHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCCC
RAYLMNTGLVGGSYGVGKRMNLPSTRKIIDEILNGNIEKSEFVTHPVFQVAYPKTISGVD
EEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHEEECCCHHCCCH
SAILDPREAWTDKAAYDQTAKKLGEMFIKNFKQYAEGSKDFDFTAFGPKI
HHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCC
>Mature Secondary Structure
MQAQTQVKGLKELGLEPSEIFHNLSYDEIYEHEKKNGETVVSSNGTMMVDTGIFTGRSPK
CCCHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHCCCCCEEEECCCEEEEEECEECCCCCC
DKYFVDEPSSNGNIWWSHINFKVSEAIFDELYKKCVNYLSHKKLYVFDGYAGANPETRVS
CCEEEECCCCCCCEEEEEECEEHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEE
LRVVSEKAWQHHFCTNMFLRPTKEELVGLDPEFTIINACGIKNENFKQHGMNSEVFVIFH
EEEEHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCCEEEEEE
LAKKICIIGGTEYGGEMKKGIFSVMNYKLPLQGILSMHCSANVGQDGDTALFFGLSGTGK
EHHCEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCC
TTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKVINLDPKTEPDIYEAIRKDALLENVV
EEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
YDPQTKIVDYSSAAKTENTRVSYPIFHINNIQVPSKGGHPKTIIFLTYDAFGVLPPVSKL
CCCCCCEEECCCCCCCCCCEEEEEEEEECCEECCCCCCCCCEEEEEEECCCCCCCCHHHH
SIEQAMYHFLSGYTAKVAGTERGIKEPTATFSACFGAAFMTLHPTKYAKLLGEKMKKHNV
HHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCCC
RAYLMNTGLVGGSYGVGKRMNLPSTRKIIDEILNGNIEKSEFVTHPVFQVAYPKTISGVD
EEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHEEECCCHHCCCH
SAILDPREAWTDKAAYDQTAKKLGEMFIKNFKQYAEGSKDFDFTAFGPKI
HHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA