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The map label for this gene is gatB

Identifier: 45656126

GI number: 45656126

Start: 250120

End: 251580

Strand: Direct

Name: gatB

Synonym: LIC10221

Alternate gene names: 45656126

Gene position: 250120-251580 (Clockwise)

Preceding gene: 45656121

Following gene: 45656127

Centisome position: 5.85

GC content: 40.38

Gene sequence:

>1461_bases
TTGGCAGAATTTGAAACGATCATAGGACTGGAAGTTCACGCTCAGCTCAATACGGAATCGAAAATATTCTCTACAAGCGC
GACTAAATTTGGTTCTCCTCCAAATTCTCAAACCAATCCGGTTTGTTTGGGGTTGCCTGGCGCATTACCGGTTCTCAACG
AATTCGCTTTAGAAAAAGCGATTATGGCCGGGCTTGCATTTGGCTGTGACATTACTCTGTTTACTAAATTCGACCGTAAG
AATTATTTTTATCCGGATCTTCCGAAAGGGTATCAAATTTCTCAGTTTGATAAACCCATTTGCACTGGTGGAGGAGTTAC
ATTTACGATCAAAGGGGAAGATTCTGCTCGTTATGTGAGACTGACTCGAATTCATCTGGAAGAAGATGCGGGCAAACTCA
TTCATTCTGCAGATCCAAATATTCCTCAGTCTTATGTAGATTTAAATAGAGCGGGGACTCCTTTGATCGAAATCGTTTCT
GAACCGGACATGCGTTCTTCAGACGAAGCGTATTATTATCTTAATTCTTTAAAATCGGTTTTGAAATACATTCGGGTTTC
GGATTGTAATATGGAAGAAGGTTCTCTTCGTTGCGACGCGAACGTTTCGATTCGCCCCAAAGGCTCGGACAAATTCGGGA
CCAGAGTTGAAATTAAAAATCTAAATTCTTTTAAAGCGGTTAAAGCCGCGATCGATTATGAAGTAGAATGGCAAAAAGAA
ATGGCTTTGGAAGGAAAAACATTTCAACAACAAACCAAACTCTGGGATTCGGTCGCTAACAAAACCGTAACGATGAGAAC
TAAGGAAATGAGCCACGACTATCGTTACTTTCCCGATCCGGATCTACCGGTTATCATCCTTCAAAAAGAAACTGTGGAGT
CGGTTCGTTCCAAACTTCCGGAACTTCCGAACGAAAGAAAAAATCGTTTTGTGGAAAAATTGGGTCTTCCTAAATACGAC
GCGGAGGTTTTAACCGCGGAAAGGGAAGTTGCGGACTATTTTGAAGACGCTTTGAAAGTTTCTGGAGATGCTAAAAAAAC
ATCCAACTGGGTTAAGGACGAAATTTTAGGTATTGTCAATAAAGAGAATATTACAATATCAGAATTTTCTGTTTCTGCTC
GTAGAATTGGCGAACTTGTAAAATTAATTGCTGATGGAAAAATTTCTGGTAAAATTGCTAAAACGGTATTTGAAGAACTT
CTTACTTCTGATAAGGACGCAGAGACTATTGTCACAGAAAAAAATCTGATTGTGGTTCGAGACGATAAAGAAATTGAAAG
GATTGTGGACGAAGCCATTGCGAATAACCAAGACGCCGTAACTAAATACAAAAGCGGAAAAGATCGCGCGTTAGGCGCCA
TTGTGGGTTATGTGATGAAGGTTTCCAAAGGAAAGGCCGATCCAGAATTGGTAAATCAGATGCTTTTAGAAAAGTTGGGT
CCGTTGCCGCCAAAAAGTTAA

Upstream 100 bases:

>100_bases
CCCGTAAATTCCGCTTTCCTCAGACTACATTTAGAGATCGTTGGATTAAGGATAGATTCCTCGATCTGTTTAGGATGACA
ATCCGGAAAAGGAGATACAG

Downstream 100 bases:

>100_bases
TAGTTTCAAAACTTTGACAGACGGCGATCTATTGGTTCTATATATATTAGAATTGTTGAAAAACTCCATAGTTCAATTAA
CAAAACTGCTTGAATCGACC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MAEFETIIGLEVHAQLNTESKIFSTSATKFGSPPNSQTNPVCLGLPGALPVLNEFALEKAIMAGLAFGCDITLFTKFDRK
NYFYPDLPKGYQISQFDKPICTGGGVTFTIKGEDSARYVRLTRIHLEEDAGKLIHSADPNIPQSYVDLNRAGTPLIEIVS
EPDMRSSDEAYYYLNSLKSVLKYIRVSDCNMEEGSLRCDANVSIRPKGSDKFGTRVEIKNLNSFKAVKAAIDYEVEWQKE
MALEGKTFQQQTKLWDSVANKTVTMRTKEMSHDYRYFPDPDLPVIILQKETVESVRSKLPELPNERKNRFVEKLGLPKYD
AEVLTAEREVADYFEDALKVSGDAKKTSNWVKDEILGIVNKENITISEFSVSARRIGELVKLIADGKISGKIAKTVFEEL
LTSDKDAETIVTEKNLIVVRDDKEIERIVDEAIANNQDAVTKYKSGKDRALGAIVGYVMKVSKGKADPELVNQMLLEKLG
PLPPKS

Sequences:

>Translated_486_residues
MAEFETIIGLEVHAQLNTESKIFSTSATKFGSPPNSQTNPVCLGLPGALPVLNEFALEKAIMAGLAFGCDITLFTKFDRK
NYFYPDLPKGYQISQFDKPICTGGGVTFTIKGEDSARYVRLTRIHLEEDAGKLIHSADPNIPQSYVDLNRAGTPLIEIVS
EPDMRSSDEAYYYLNSLKSVLKYIRVSDCNMEEGSLRCDANVSIRPKGSDKFGTRVEIKNLNSFKAVKAAIDYEVEWQKE
MALEGKTFQQQTKLWDSVANKTVTMRTKEMSHDYRYFPDPDLPVIILQKETVESVRSKLPELPNERKNRFVEKLGLPKYD
AEVLTAEREVADYFEDALKVSGDAKKTSNWVKDEILGIVNKENITISEFSVSARRIGELVKLIADGKISGKIAKTVFEEL
LTSDKDAETIVTEKNLIVVRDDKEIERIVDEAIANNQDAVTKYKSGKDRALGAIVGYVMKVSKGKADPELVNQMLLEKLG
PLPPKS
>Mature_485_residues
AEFETIIGLEVHAQLNTESKIFSTSATKFGSPPNSQTNPVCLGLPGALPVLNEFALEKAIMAGLAFGCDITLFTKFDRKN
YFYPDLPKGYQISQFDKPICTGGGVTFTIKGEDSARYVRLTRIHLEEDAGKLIHSADPNIPQSYVDLNRAGTPLIEIVSE
PDMRSSDEAYYYLNSLKSVLKYIRVSDCNMEEGSLRCDANVSIRPKGSDKFGTRVEIKNLNSFKAVKAAIDYEVEWQKEM
ALEGKTFQQQTKLWDSVANKTVTMRTKEMSHDYRYFPDPDLPVIILQKETVESVRSKLPELPNERKNRFVEKLGLPKYDA
EVLTAEREVADYFEDALKVSGDAKKTSNWVKDEILGIVNKENITISEFSVSARRIGELVKLIADGKISGKIAKTVFEELL
TSDKDAETIVTEKNLIVVRDDKEIERIVDEAIANNQDAVTKYKSGKDRALGAIVGYVMKVSKGKADPELVNQMLLEKLGP
LPPKS

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=497, Percent_Identity=35.2112676056338, Blast_Score=311, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI115534094, Length=478, Percent_Identity=30.3347280334728, Blast_Score=207, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6319391, Length=506, Percent_Identity=30.0395256916996, Blast_Score=222, Evalue=1e-58,
Organism=Drosophila melanogaster, GI45550808, Length=495, Percent_Identity=35.3535353535354, Blast_Score=302, Evalue=3e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54319; Mature: 54187

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFETIIGLEVHAQLNTESKIFSTSATKFGSPPNSQTNPVCLGLPGALPVLNEFALEKA
CCCCCEEEEEEEEEEECCCCCEEECCCHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
IMAGLAFGCDITLFTKFDRKNYFYPDLPKGYQISQFDKPICTGGGVTFTIKGEDSARYVR
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEECCCCCEEEE
LTRIHLEEDAGKLIHSADPNIPQSYVDLNRAGTPLIEIVSEPDMRSSDEAYYYLNSLKSV
EEEEEEHHHHHHHHHCCCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHH
LKYIRVSDCNMEEGSLRCDANVSIRPKGSDKFGTRVEIKNLNSFKAVKAAIDYEVEWQKE
HHHHHHCCCCCCCCCEEECCCEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCEEEHHH
MALEGKTFQQQTKLWDSVANKTVTMRTKEMSHDYRYFPDPDLPVIILQKETVESVRSKLP
HHCCCCHHHHHHHHHHHHHCCEEEEEEHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHCC
ELPNERKNRFVEKLGLPKYDAEVLTAEREVADYFEDALKVSGDAKKTSNWVKDEILGIVN
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
KENITISEFSVSARRIGELVKLIADGKISGKIAKTVFEELLTSDKDAETIVTEKNLIVVR
CCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCEEEEE
DDKEIERIVDEAIANNQDAVTKYKSGKDRALGAIVGYVMKVSKGKADPELVNQMLLEKLG
CCHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
PLPPKS
CCCCCC
>Mature Secondary Structure 
AEFETIIGLEVHAQLNTESKIFSTSATKFGSPPNSQTNPVCLGLPGALPVLNEFALEKA
CCCCEEEEEEEEEEECCCCCEEECCCHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
IMAGLAFGCDITLFTKFDRKNYFYPDLPKGYQISQFDKPICTGGGVTFTIKGEDSARYVR
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEECCCCCEEEE
LTRIHLEEDAGKLIHSADPNIPQSYVDLNRAGTPLIEIVSEPDMRSSDEAYYYLNSLKSV
EEEEEEHHHHHHHHHCCCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHH
LKYIRVSDCNMEEGSLRCDANVSIRPKGSDKFGTRVEIKNLNSFKAVKAAIDYEVEWQKE
HHHHHHCCCCCCCCCEEECCCEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCEEEHHH
MALEGKTFQQQTKLWDSVANKTVTMRTKEMSHDYRYFPDPDLPVIILQKETVESVRSKLP
HHCCCCHHHHHHHHHHHHHCCEEEEEEHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHCC
ELPNERKNRFVEKLGLPKYDAEVLTAEREVADYFEDALKVSGDAKKTSNWVKDEILGIVN
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
KENITISEFSVSARRIGELVKLIADGKISGKIAKTVFEELLTSDKDAETIVTEKNLIVVR
CCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCEEEEE
DDKEIERIVDEAIANNQDAVTKYKSGKDRALGAIVGYVMKVSKGKADPELVNQMLLEKLG
CCHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
PLPPKS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA