Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 45656065

GI number: 45656065

Start: 188028

End: 189398

Strand: Direct

Name: trmE

Synonym: LIC10159

Alternate gene names: 45656065

Gene position: 188028-189398 (Clockwise)

Preceding gene: 45656064

Following gene: 45656066

Centisome position: 4.4

GC content: 37.56

Gene sequence:

>1371_bases
TTGAATGATACGATAGCCGCTGTATCTACTTCTTCCGGTGCCGGAGCCATCGGAATCATCCGAATGTCCGGTCCGGAAGC
GCTTACGATTTCCTCTTCTTTTCTTTTTTCGAAAAATAAATTTCTCTCTCCCTCCGAAATTCTACCTAGAACGGCAATTC
AATGCGTTTTTCAAATCGGCGATCGAAAGATAGATCAAATATTATTCTTTTATTTTAAGTCTCCGAATTCTTATACGGGC
GAAGATCTTTGTGAATTTCACTTTCACGGAAATCCAATTTTGTTAAGAGAAGCGTTAGACGCCATCTTTAGAGCGGGTGC
TCGTCCTGCAAAACAAGGTGAGTTTTCTCGTAGAGCGTTTTTAAACGAAAAGTTGGATCTGACCGAAGTTGAGGCAATCG
GTAGACTTATTTCTGCACGTTCTCGTTTTGAATTGGAACTTGCACAAAAGAACGTTTTTGGTGAAGTTACTCGTTTTACT
TCCAATTTAAGAAGCCAGTTGATTTCTCTCAAAGCGGAATGTGAAGCGGAGATTGATTTTTCCACAGAAGATCTCACGTA
TGAATCTTTGGAAGAAAGAAAGACTCGGATTGAAAATGTTAAATCTCTTTGTCAAACTTTGATTTCAAAATCAAGTTCTG
CGGAAAAATTAATTCAGCAATTTAGAATTGTTCTCTATGGAGAACCAAATACCGGTAAGTCTAGCCTGATGAACGTTCTT
CTTGGTAAAGAACGTTCTATCATTTCTGAAATCCCTGGAACGACTCGGGATTATATCAGTGAAGAAATTTTTCTCGAAGG
AATTCCTGTTCGACTTGTGGACACTGCTGGTGTTAGAGAAACGACGGACCATATCGAAAAACTAGGAATCGAAAGAAGTG
AAAAAGAATTTCAATCCGCTGATGTTCGACTTTTTCTCGTAGACGTTTCTAAAAAAGAAAATTGGAAAGAGTTTATTAAT
AAATCTAGAGAAAGATTAGAAGGTTCTATTCTTATCGCAAATAAAATTGATATTTTGAATTCTTCCTGGGACCGAAATTT
ATTTTCAGACGTAAAAGATTTAATCGTACTAGAAATTTCCTGTAAAACCAAAGAAGGAATTTCAAACCTTTTAGATGCGA
TCAAAGAAAGGACGGGTAAACTAGGTCATTCTGAAGATTATGTCCTTTTGGAAGAACGTCAAAGATATCATTTCGAAACC
ATTGTCAGATGTCTTGATAAAACCCTCCATTTGCTTAAAGAAGGAGCTCCTGCGGAGATTTATATTCAAGAAATCAATTA
TGCTCTTGCCGAAATCGGAGAGGTTAACGGTAAAGTGGATACGGAAGAAGTTCTCGGGAGAATCTTCAGTAAGTTCTGCG
TTGGTAAATGA

Upstream 100 bases:

>100_bases
ACTTACAAAAAAGTAAGAATCATTCCGATGAAAGATAAACACAAATACAAAGACGTTGTCGAAAAAGGTCCAAACAACGA
CCTTCTCGAAGAAGTGAACT

Downstream 100 bases:

>100_bases
AATTTTATCGTCGCAAGGTTGAATTTGAAAAACATGGGAAAAATCGTATGAGGGAAAAATCGTTACAATATCACGCGCTT
CATCCTAAGGGAAAAATTGA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MNDTIAAVSTSSGAGAIGIIRMSGPEALTISSSFLFSKNKFLSPSEILPRTAIQCVFQIGDRKIDQILFFYFKSPNSYTG
EDLCEFHFHGNPILLREALDAIFRAGARPAKQGEFSRRAFLNEKLDLTEVEAIGRLISARSRFELELAQKNVFGEVTRFT
SNLRSQLISLKAECEAEIDFSTEDLTYESLEERKTRIENVKSLCQTLISKSSSAEKLIQQFRIVLYGEPNTGKSSLMNVL
LGKERSIISEIPGTTRDYISEEIFLEGIPVRLVDTAGVRETTDHIEKLGIERSEKEFQSADVRLFLVDVSKKENWKEFIN
KSRERLEGSILIANKIDILNSSWDRNLFSDVKDLIVLEISCKTKEGISNLLDAIKERTGKLGHSEDYVLLEERQRYHFET
IVRCLDKTLHLLKEGAPAEIYIQEINYALAEIGEVNGKVDTEEVLGRIFSKFCVGK

Sequences:

>Translated_456_residues
MNDTIAAVSTSSGAGAIGIIRMSGPEALTISSSFLFSKNKFLSPSEILPRTAIQCVFQIGDRKIDQILFFYFKSPNSYTG
EDLCEFHFHGNPILLREALDAIFRAGARPAKQGEFSRRAFLNEKLDLTEVEAIGRLISARSRFELELAQKNVFGEVTRFT
SNLRSQLISLKAECEAEIDFSTEDLTYESLEERKTRIENVKSLCQTLISKSSSAEKLIQQFRIVLYGEPNTGKSSLMNVL
LGKERSIISEIPGTTRDYISEEIFLEGIPVRLVDTAGVRETTDHIEKLGIERSEKEFQSADVRLFLVDVSKKENWKEFIN
KSRERLEGSILIANKIDILNSSWDRNLFSDVKDLIVLEISCKTKEGISNLLDAIKERTGKLGHSEDYVLLEERQRYHFET
IVRCLDKTLHLLKEGAPAEIYIQEINYALAEIGEVNGKVDTEEVLGRIFSKFCVGK
>Mature_456_residues
MNDTIAAVSTSSGAGAIGIIRMSGPEALTISSSFLFSKNKFLSPSEILPRTAIQCVFQIGDRKIDQILFFYFKSPNSYTG
EDLCEFHFHGNPILLREALDAIFRAGARPAKQGEFSRRAFLNEKLDLTEVEAIGRLISARSRFELELAQKNVFGEVTRFT
SNLRSQLISLKAECEAEIDFSTEDLTYESLEERKTRIENVKSLCQTLISKSSSAEKLIQQFRIVLYGEPNTGKSSLMNVL
LGKERSIISEIPGTTRDYISEEIFLEGIPVRLVDTAGVRETTDHIEKLGIERSEKEFQSADVRLFLVDVSKKENWKEFIN
KSRERLEGSILIANKIDILNSSWDRNLFSDVKDLIVLEISCKTKEGISNLLDAIKERTGKLGHSEDYVLLEERQRYHFET
IVRCLDKTLHLLKEGAPAEIYIQEINYALAEIGEVNGKVDTEEVLGRIFSKFCVGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=480, Percent_Identity=31.6666666666667, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI193082993, Length=466, Percent_Identity=32.1888412017167, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI193082988, Length=512, Percent_Identity=30.078125, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI2367268, Length=463, Percent_Identity=34.341252699784, Blast_Score=261, Evalue=7e-71,
Organism=Escherichia coli, GI87082120, Length=175, Percent_Identity=30.2857142857143, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=465, Percent_Identity=32.9032258064516, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323665, Length=508, Percent_Identity=31.6929133858268, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI45550813, Length=483, Percent_Identity=30.0207039337474, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_LEPIC (Q72VY6)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000151.1
- ProteinModelPortal:   Q72VY6
- SMR:   Q72VY6
- GeneID:   2771935
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10159
- NMPDR:   fig|267671.1.peg.151
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   LINT267671:LIC_10159-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51534; Mature: 51534

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: BINDING 21-21 BINDING 85-85 BINDING 124-124 BINDING 456-456

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTIAAVSTSSGAGAIGIIRMSGPEALTISSSFLFSKNKFLSPSEILPRTAIQCVFQIG
CCCCEEEEECCCCCCEEEEEEECCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
DRKIDQILFFYFKSPNSYTGEDLCEFHFHGNPILLREALDAIFRAGARPAKQGEFSRRAF
CHHHHHHEEEEECCCCCCCCCHHHEEEECCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHH
LNEKLDLTEVEAIGRLISARSRFELELAQKNVFGEVTRFTSNLRSQLISLKAECEAEIDF
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCC
STEDLTYESLEERKTRIENVKSLCQTLISKSSSAEKLIQQFRIVLYGEPNTGKSSLMNVL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCEEEEEECCCCCHHHHHHHH
LGKERSIISEIPGTTRDYISEEIFLEGIPVRLVDTAGVRETTDHIEKLGIERSEKEFQSA
HCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHCC
DVRLFLVDVSKKENWKEFINKSRERLEGSILIANKIDILNSSWDRNLFSDVKDLIVLEIS
CEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECHHHHCCCCCHHHHHHHHHHEEEEEE
CKTKEGISNLLDAIKERTGKLGHSEDYVLLEERQRYHFETIVRCLDKTLHLLKEGAPAEI
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
YIQEINYALAEIGEVNGKVDTEEVLGRIFSKFCVGK
EHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDTIAAVSTSSGAGAIGIIRMSGPEALTISSSFLFSKNKFLSPSEILPRTAIQCVFQIG
CCCCEEEEECCCCCCEEEEEEECCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
DRKIDQILFFYFKSPNSYTGEDLCEFHFHGNPILLREALDAIFRAGARPAKQGEFSRRAF
CHHHHHHEEEEECCCCCCCCCHHHEEEECCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHH
LNEKLDLTEVEAIGRLISARSRFELELAQKNVFGEVTRFTSNLRSQLISLKAECEAEIDF
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCC
STEDLTYESLEERKTRIENVKSLCQTLISKSSSAEKLIQQFRIVLYGEPNTGKSSLMNVL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCEEEEEECCCCCHHHHHHHH
LGKERSIISEIPGTTRDYISEEIFLEGIPVRLVDTAGVRETTDHIEKLGIERSEKEFQSA
HCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHCC
DVRLFLVDVSKKENWKEFINKSRERLEGSILIANKIDILNSSWDRNLFSDVKDLIVLEIS
CEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECHHHHCCCCCHHHHHHHHHHEEEEEE
CKTKEGISNLLDAIKERTGKLGHSEDYVLLEERQRYHFETIVRCLDKTLHLLKEGAPAEI
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
YIQEINYALAEIGEVNGKVDTEEVLGRIFSKFCVGK
EHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA