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The map label for this gene is maeB

Identifier: 45656066

GI number: 45656066

Start: 189395

End: 190708

Strand: Direct

Name: maeB

Synonym: LIC10160

Alternate gene names: 45656066

Gene position: 189395-190708 (Clockwise)

Preceding gene: 45656065

Following gene: 45656067

Centisome position: 4.43

GC content: 38.66

Gene sequence:

>1314_bases
ATGAAATTTTATCGTCGCAAGGTTGAATTTGAAAAACATGGGAAAAATCGTATGAGGGAAAAATCGTTACAATATCACGC
GCTTCATCCTAAGGGAAAAATTGAAGTAGTTCCTACAAAACCTACTGAAGATTCTTACGATCTTTCTCTGGCTTATTCTC
CCGGTGTCGCTTATCCTTGTTTGGAAATTAAGGATTCTCCCGAACTCGTTTATGAATATACAAACAAAGGAAATCTTGTT
GGGATCATTACAAACGGGACCGCCATTTTAGGATTAGGTGATATAGGTGCTCTTGCCGGTAAGCCGGTTATGGAAGGAAA
GGCGGTTCTTTTTAAAAAATTTGCGGGGATAGACGTATTTGATATAGAGATCAATACAAAAGATCCGGATGAATTCATTC
GTGCGGTGAAACTTCTGGAGCCAACTTTTGGCGGTATTAATCTAGAAGATATTAAAGCTCCTGAAAGTTTTTATATTGAA
GAACAACTTATCGAAAAGATGAACATTCCTGTTTTTCACGACGATCAACATGGAACCGCGATCATCACTGTCGCTGGTTT
ATTGAACTCGTTTAAACTCACCGGTAAAAATCCGGACGACGTTAAAGTAGTGATTTGTGGAGCGGGTGCTGCAGGAATTG
CAATTGCAGAACTCATTCAACATATAGGAATCAAAAAAGAGCAGATTTTTCTGGTCGATACCAAAGGTGTGATTCATCAT
AATAGAAACGATCTAAACGATTCTAAAAAGAAGTATATTCAAAAAACAGATTCTACCACACTCAGAGATGTAATGAAAGG
TTCAGATATTTTTATTGGTGTTTCCGTTGAGAATATGGTAGATGAAGATATGGTTCGTTCTATGTCTAAAAATCCAGTGA
TCTTTGCTCTTGCAAATCCTGATCCCGAAATCCCATATCGTGTTGCAAAGGCAGTTCGTTCCGATTTAATTATGGGAACT
GGAAGAAGTGATAATCCAAATCAGGTGAATAACGTATTAGGATTTCCTTTTATTTTTAGAGGCGCTCTAGATGTTAGAGC
TCGTCATATTACTTTAGAGATGAAACTTGCGGCCGCTCGTTCTTTGGCGGAATTGGCTCGTTTGGAAGTTCCTGAAGTGG
TTAGTAAAGCTTATGGCGGAGAGAAATTTACGTTTGGTCCGGAATATCTGATTCCAAAACCTTTTGATAAACGAGTTTTA
TTTCATGTGGCCCCGGCAGTTGCAGAGGCTGCAGTTGCAAGTGGCTCTGCCCGTATTCCTTATTTGGGAAGAGAAAAATA
TTTAGGTTTTCTCGAAGGAATTATTAAAAGTTAG

Upstream 100 bases:

>100_bases
AAGAAATCAATTATGCTCTTGCCGAAATCGGAGAGGTTAACGGTAAAGTGGATACGGAAGAAGTTCTCGGGAGAATCTTC
AGTAAGTTCTGCGTTGGTAA

Downstream 100 bases:

>100_bases
CATTGTCCAATTTTGTTTTTAGAACGAAATAACAAATAGAATTGTTGAAAAATTAATTCTCCATCTGTTTCTATTTTATG
GAAATGGGCAATTAAAGCAG

Product: malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MKFYRRKVEFEKHGKNRMREKSLQYHALHPKGKIEVVPTKPTEDSYDLSLAYSPGVAYPCLEIKDSPELVYEYTNKGNLV
GIITNGTAILGLGDIGALAGKPVMEGKAVLFKKFAGIDVFDIEINTKDPDEFIRAVKLLEPTFGGINLEDIKAPESFYIE
EQLIEKMNIPVFHDDQHGTAIITVAGLLNSFKLTGKNPDDVKVVICGAGAAGIAIAELIQHIGIKKEQIFLVDTKGVIHH
NRNDLNDSKKKYIQKTDSTTLRDVMKGSDIFIGVSVENMVDEDMVRSMSKNPVIFALANPDPEIPYRVAKAVRSDLIMGT
GRSDNPNQVNNVLGFPFIFRGALDVRARHITLEMKLAAARSLAELARLEVPEVVSKAYGGEKFTFGPEYLIPKPFDKRVL
FHVAPAVAEAAVASGSARIPYLGREKYLGFLEGIIKS

Sequences:

>Translated_437_residues
MKFYRRKVEFEKHGKNRMREKSLQYHALHPKGKIEVVPTKPTEDSYDLSLAYSPGVAYPCLEIKDSPELVYEYTNKGNLV
GIITNGTAILGLGDIGALAGKPVMEGKAVLFKKFAGIDVFDIEINTKDPDEFIRAVKLLEPTFGGINLEDIKAPESFYIE
EQLIEKMNIPVFHDDQHGTAIITVAGLLNSFKLTGKNPDDVKVVICGAGAAGIAIAELIQHIGIKKEQIFLVDTKGVIHH
NRNDLNDSKKKYIQKTDSTTLRDVMKGSDIFIGVSVENMVDEDMVRSMSKNPVIFALANPDPEIPYRVAKAVRSDLIMGT
GRSDNPNQVNNVLGFPFIFRGALDVRARHITLEMKLAAARSLAELARLEVPEVVSKAYGGEKFTFGPEYLIPKPFDKRVL
FHVAPAVAEAAVASGSARIPYLGREKYLGFLEGIIKS
>Mature_437_residues
MKFYRRKVEFEKHGKNRMREKSLQYHALHPKGKIEVVPTKPTEDSYDLSLAYSPGVAYPCLEIKDSPELVYEYTNKGNLV
GIITNGTAILGLGDIGALAGKPVMEGKAVLFKKFAGIDVFDIEINTKDPDEFIRAVKLLEPTFGGINLEDIKAPESFYIE
EQLIEKMNIPVFHDDQHGTAIITVAGLLNSFKLTGKNPDDVKVVICGAGAAGIAIAELIQHIGIKKEQIFLVDTKGVIHH
NRNDLNDSKKKYIQKTDSTTLRDVMKGSDIFIGVSVENMVDEDMVRSMSKNPVIFALANPDPEIPYRVAKAVRSDLIMGT
GRSDNPNQVNNVLGFPFIFRGALDVRARHITLEMKLAAARSLAELARLEVPEVVSKAYGGEKFTFGPEYLIPKPFDKRVL
FHVAPAVAEAAVASGSARIPYLGREKYLGFLEGIIKS

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=395, Percent_Identity=26.0759493670886, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI62420880, Length=395, Percent_Identity=26.0759493670886, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI239049447, Length=395, Percent_Identity=26.0759493670886, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI4505143, Length=350, Percent_Identity=28.2857142857143, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4505145, Length=345, Percent_Identity=26.9565217391304, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788806, Length=415, Percent_Identity=57.8313253012048, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI87081919, Length=405, Percent_Identity=27.1604938271605, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17537199, Length=355, Percent_Identity=28.4507042253521, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322823, Length=347, Percent_Identity=27.0893371757925, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281362674, Length=396, Percent_Identity=26.7676767676768, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281362672, Length=396, Percent_Identity=26.7676767676768, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356279, Length=396, Percent_Identity=26.7676767676768, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI78707242, Length=345, Percent_Identity=28.1159420289855, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI78707238, Length=345, Percent_Identity=28.1159420289855, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI78707240, Length=345, Percent_Identity=28.1159420289855, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24646388, Length=401, Percent_Identity=26.6832917705736, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24646386, Length=401, Percent_Identity=26.6832917705736, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281363503, Length=358, Percent_Identity=25.4189944134078, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281363505, Length=358, Percent_Identity=25.4189944134078, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI78707236, Length=358, Percent_Identity=25.4189944134078, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI78707232, Length=358, Percent_Identity=25.4189944134078, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 48258; Mature: 48258

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFYRRKVEFEKHGKNRMREKSLQYHALHPKGKIEVVPTKPTEDSYDLSLAYSPGVAYPC
CCCHHHHCCHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCCE
LEIKDSPELVYEYTNKGNLVGIITNGTAILGLGDIGALAGKPVMEGKAVLFKKFAGIDVF
EEECCCCCEEEEECCCCCEEEEEECCEEEEEECCHHHHCCCCCCCCHHHHHHHHCCCEEE
DIEINTKDPDEFIRAVKLLEPTFGGINLEDIKAPESFYIEEQLIEKMNIPVFHDDQHGTA
EEEECCCCHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCEE
IITVAGLLNSFKLTGKNPDDVKVVICGAGAAGIAIAELIQHIGIKKEQIFLVDTKGVIHH
EEEEEHHHCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEEC
NRNDLNDSKKKYIQKTDSTTLRDVMKGSDIFIGVSVENMVDEDMVRSMSKNPVIFALANP
CCCCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCCCEEEEECCC
DPEIPYRVAKAVRSDLIMGTGRSDNPNQVNNVLGFPFIFRGALDVRARHITLEMKLAAAR
CCCCCHHHHHHHHHHHEEECCCCCCCHHHHHHCCCHHHHHCHHEEEEEEEEEEEHHHHHH
SLAELARLEVPEVVSKAYGGEKFTFGPEYLIPKPFDKRVLFHVAPAVAEAAVASGSARIP
HHHHHHHCCHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
YLGREKYLGFLEGIIKS
CCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFYRRKVEFEKHGKNRMREKSLQYHALHPKGKIEVVPTKPTEDSYDLSLAYSPGVAYPC
CCCHHHHCCHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCCE
LEIKDSPELVYEYTNKGNLVGIITNGTAILGLGDIGALAGKPVMEGKAVLFKKFAGIDVF
EEECCCCCEEEEECCCCCEEEEEECCEEEEEECCHHHHCCCCCCCCHHHHHHHHCCCEEE
DIEINTKDPDEFIRAVKLLEPTFGGINLEDIKAPESFYIEEQLIEKMNIPVFHDDQHGTA
EEEECCCCHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCEE
IITVAGLLNSFKLTGKNPDDVKVVICGAGAAGIAIAELIQHIGIKKEQIFLVDTKGVIHH
EEEEEHHHCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEEC
NRNDLNDSKKKYIQKTDSTTLRDVMKGSDIFIGVSVENMVDEDMVRSMSKNPVIFALANP
CCCCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCCCEEEEECCC
DPEIPYRVAKAVRSDLIMGTGRSDNPNQVNNVLGFPFIFRGALDVRARHITLEMKLAAAR
CCCCCHHHHHHHHHHHEEECCCCCCCHHHHHHCCCHHHHHCHHEEEEEEEEEEEHHHHHH
SLAELARLEVPEVVSKAYGGEKFTFGPEYLIPKPFDKRVLFHVAPAVAEAAVASGSARIP
HHHHHHHCCHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
YLGREKYLGFLEGIIKS
CCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]