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The map label for this gene is hacB [H]

Identifier: 45357944

GI number: 45357944

Start: 379027

End: 379512

Strand: Direct

Name: hacB [H]

Synonym: MMP0381

Alternate gene names: 45357944

Gene position: 379027-379512 (Clockwise)

Preceding gene: 45357938

Following gene: 45357945

Centisome position: 22.82

GC content: 36.83

Gene sequence:

>486_bases
ATGAAAATAACTGGTAAGGTGCACTTATTTGGGGATGACATCGATACTGATGCGATAATTCCCGGAGCTTATTTAAAAAC
GACTGATGAATATGAGCTTGCATCGCACTGTATGGCAGGAATTGACGAAAATTTTCCAGAAAGGGTCGAAGATGGTGACT
TTTTAGTTGCAGGTGAAAATTTTGGATGCGGAAGTTCAAGGGAACAGGCCCCAATTGCCATAAAATACTGCGGAATCAAG
GCAATAATTGTTGAGAGTTTTGCAAGGATATTTTACAGAAATTGCATAAATTTAGGAGTATTTCCAATTGAATGCAAGGG
AATATCAAAACACGTCAAAGATGGGGATGTAATAGAATTAGATCTTGAAGAAAAAAAAGTTATCTTAAAAGACACGGTTC
TTGACTGCAATCTTCCGACAGGGACTGCAAAAGATATAATGGATGAAGGCGGGCTTATAAATTACGCAAAGAAACAAAAA
AATTAA

Upstream 100 bases:

>100_bases
TATGTTCATTAAAGTAATATTTGAGAGTTACAGTTTTTAGTATATTTTTAATTTAAATTCGGAAAAATTCGGTTTTATTT
TAGTTATTGATGGTGACATG

Downstream 100 bases:

>100_bases
AATTTAAACGATTTAACAAATTTGATACAAATTAATTAGATAGTAATCAGATAGTAAATTTATATTTTGGTGATTTTAAT
GTCGCGACTGGCGATTTTAG

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: HACN; (R)-homocitrate dehydratase; Homoaconitate hydratase [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MKITGKVHLFGDDIDTDAIIPGAYLKTTDEYELASHCMAGIDENFPERVEDGDFLVAGENFGCGSSREQAPIAIKYCGIK
AIIVESFARIFYRNCINLGVFPIECKGISKHVKDGDVIELDLEEKKVILKDTVLDCNLPTGTAKDIMDEGGLINYAKKQK
N

Sequences:

>Translated_161_residues
MKITGKVHLFGDDIDTDAIIPGAYLKTTDEYELASHCMAGIDENFPERVEDGDFLVAGENFGCGSSREQAPIAIKYCGIK
AIIVESFARIFYRNCINLGVFPIECKGISKHVKDGDVIELDLEEKKVILKDTVLDCNLPTGTAKDIMDEGGLINYAKKQK
N
>Mature_161_residues
MKITGKVHLFGDDIDTDAIIPGAYLKTTDEYELASHCMAGIDENFPERVEDGDFLVAGENFGCGSSREQAPIAIKYCGIK
AIIVESFARIFYRNCINLGVFPIECKGISKHVKDGDVIELDLEEKKVILKDTVLDCNLPTGTAKDIMDEGGLINYAKKQK
N

Specific function: Hydro-lyase with broad substrate specificity for cis- unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4- tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4- tricarboxyla

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=122, Percent_Identity=36.8852459016393, Blast_Score=72, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=172, Percent_Identity=31.3953488372093, Blast_Score=82, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=107, Percent_Identity=40.1869158878505, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.36 [H]

Molecular weight: Translated: 17735; Mature: 17735

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITGKVHLFGDDIDTDAIIPGAYLKTTDEYELASHCMAGIDENFPERVEDGDFLVAGEN
CCCCEEEEEECCCCCCCEECCCCCEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCC
FGCGSSREQAPIAIKYCGIKAIIVESFARIFYRNCINLGVFPIECKGISKHVKDGDVIEL
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEE
DLEEKKVILKDTVLDCNLPTGTAKDIMDEGGLINYAKKQKN
ECCCCEEEEEEEEEECCCCCCCHHHHHHCCCEEECHHCCCC
>Mature Secondary Structure
MKITGKVHLFGDDIDTDAIIPGAYLKTTDEYELASHCMAGIDENFPERVEDGDFLVAGEN
CCCCEEEEEECCCCCCCEECCCCCEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCC
FGCGSSREQAPIAIKYCGIKAIIVESFARIFYRNCINLGVFPIECKGISKHVKDGDVIEL
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEE
DLEEKKVILKDTVLDCNLPTGTAKDIMDEGGLINYAKKQKN
ECCCCEEEEEEEEEECCCCCCCHHHHHHCCCEEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]