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The map label for this gene is wbpM [H]

Identifier: 42523197

GI number: 42523197

Start: 1636089

End: 1637969

Strand: Direct

Name: wbpM [H]

Synonym: Bd1700

Alternate gene names: 42523197

Gene position: 1636089-1637969 (Clockwise)

Preceding gene: 42523193

Following gene: 42523198

Centisome position: 43.25

GC content: 48.48

Gene sequence:

>1881_bases
ATGATCCATCACTGGTTTTATAAGGAATCTCAGATGTTTGTGCGTATTGCGGGGCTGCCGCGAAGAATTAAGATTTTCAT
CATGCTTTTCTGCGACGTCATTCTTCTTCCGCTGGCGTTGTATTCAGCAATAGCTTTGCGGTTGGGTACAATTCGTCCCG
AAGTTTCTCCGTTTTGGTGGCTGTTTCTTGCTATTCCATTTCTGACTGTTCCCATCTTTGTTCGTGTCGGTTTGTATCGC
GCAGTTATCCGATACATGGATGATCGAATTATTGCTACTGTCTTTTATGGTGTGAGTCTTTCTGTGTTGCTGCTTACCGC
GGCCGTAGTAATGGGAAATGTTACGGGTGTTCCGCGTTCATCCATAGTGATTTACTGGATTATTGCGATTGCTTATATTA
CTTCGAGTCGTTTTATCGCCCGAGGTGTGTTGCGCTCTTTAGAGCGAGAGCAAGATCGCAGGCAACCCGTAGCTATTTAC
GGTGCAGGCCGATCTGGTTTGCAAACGGCTTTGGCACTGATGTCCGGTCCTGAGTTCAGGCCGGTTGCATTCTTCGACGA
TAATAGACAGCTGCACGGGACTAGTGTTGCTGGTATCCGCGTTTACTCTCCTGACGAAGCTTTGAATATCATGGGTGCTG
AGGACTGCAATCAGCTTCTCATCGCAATGCCTTCAGCAAGCAGATCCCGCCGCCGTGAAATCATCCAACGTTTCGAAAAT
GTCGACATTCGCTTAAAAACTCTTCCAGGCATTGGCGAACTTGTCGATGGTAAGGTCCGTATCGAAGACATCCGCGAGGT
GGGCGTCGAGGATCTGTTGGGCCGCGATCCTGTTCCACCGTTTGAAGACCTGATTAAGTCATGCATTCGCGATAAGGTCG
TATTGGTGACAGGGGCGGGCGGATCTATTGGCTCTGAGTTGTGTCGGCAGATCATCTTGAACACCCCGAAGAAGCTGATC
ATCTTTGAGCAGGCCGAGTACGCACTGTACAAGATCGAGCAAGATATCCTTAAGCACCGTATTAATTTTGAAGTTGTGCC
GGTTCTGGGTGATGTTCTAAATACCGAACATTTAGAAAAAATTATCGCAAGCCACGGAGTTCAAACGATCTATCACGCGG
CTGCCTATAAACACGTGCCCCTTGTTGAATCCAATGTCATTGCGGGCATTGTGAACAATGTCTTTGGAACCCTGTCGGCA
TCAAAAGCGGCTATCAAATGTAAGGTAGAAACCTTTGTTCTGATTTCCACCGACAAGGCGGTTCGACCAACAAACATTAT
GGGTGCTTCAAAACGTCTGGCCGAGCTGGTTTTGCAAGGGTTGTCTCAGGATAAGTCCCATTCTACGCGTTTTTGCATGG
TTCGTTTCGGAAATGTACTAGGGTCCTCCGGCTCTGTCGTACCTTTGTTTAAAGAACAGATTCGTCACGGGGGCCCGGTT
ACTGTAACTCATCCCGATATCACTCGTTACTTCATGACCATCCCCGAAGCGGCTCAGCTTGTTTTGCAGGCCGGAGCCAT
GGGTAAGGGCGGGGATGTCTTCGTGCTTGATATGGGCGAATCAGTAAAGATCGTGGATCTTGCAAAAAAGATGATCGAGC
TCAGTGGTCTTGAGGTTCGAAACCCAGATACCGGACAAGGTGATATCAGTATAGAATTCACCGGACTTCGCCCTGGCGAA
AAGCTGTATGAAGAATTGCTTATCGGTGAAAACGTAAGCTGGACTGCTCACCCTCGAATCATGACAGCGGAAGAGGCTTC
GATCGAATTGTCTAAGCTTATGCAGTCTTTAGATCAGATGCGCAGCGCATGCAGTACGGGTGATGAGCCTGCCGTGCGCG
AGGTGCTAAAAAGATTGGTGCCGGAGTATAAGCCGACTTAA

Upstream 100 bases:

>100_bases
CTAGAGGTCTGTGTCAATGTTGATATCTGATTGTGAGTTCTTTGATGGGGGTGCGCCATAGCCTAGATTTGAAATATTGT
AGATCAAAGTTCCTTATGTA

Downstream 100 bases:

>100_bases
GTGCTTAAGTGGCAAGTGTGATCGGACGATTGCTGGCGAGCGCTAGCGTCACAGTCTTAAGTTGAAATCATATTTCAACT
AAACAACAATATAGTTTCAT

Product: nucleotide sugar epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MIHHWFYKESQMFVRIAGLPRRIKIFIMLFCDVILLPLALYSAIALRLGTIRPEVSPFWWLFLAIPFLTVPIFVRVGLYR
AVIRYMDDRIIATVFYGVSLSVLLLTAAVVMGNVTGVPRSSIVIYWIIAIAYITSSRFIARGVLRSLEREQDRRQPVAIY
GAGRSGLQTALALMSGPEFRPVAFFDDNRQLHGTSVAGIRVYSPDEALNIMGAEDCNQLLIAMPSASRSRRREIIQRFEN
VDIRLKTLPGIGELVDGKVRIEDIREVGVEDLLGRDPVPPFEDLIKSCIRDKVVLVTGAGGSIGSELCRQIILNTPKKLI
IFEQAEYALYKIEQDILKHRINFEVVPVLGDVLNTEHLEKIIASHGVQTIYHAAAYKHVPLVESNVIAGIVNNVFGTLSA
SKAAIKCKVETFVLISTDKAVRPTNIMGASKRLAELVLQGLSQDKSHSTRFCMVRFGNVLGSSGSVVPLFKEQIRHGGPV
TVTHPDITRYFMTIPEAAQLVLQAGAMGKGGDVFVLDMGESVKIVDLAKKMIELSGLEVRNPDTGQGDISIEFTGLRPGE
KLYEELLIGENVSWTAHPRIMTAEEASIELSKLMQSLDQMRSACSTGDEPAVREVLKRLVPEYKPT

Sequences:

>Translated_626_residues
MIHHWFYKESQMFVRIAGLPRRIKIFIMLFCDVILLPLALYSAIALRLGTIRPEVSPFWWLFLAIPFLTVPIFVRVGLYR
AVIRYMDDRIIATVFYGVSLSVLLLTAAVVMGNVTGVPRSSIVIYWIIAIAYITSSRFIARGVLRSLEREQDRRQPVAIY
GAGRSGLQTALALMSGPEFRPVAFFDDNRQLHGTSVAGIRVYSPDEALNIMGAEDCNQLLIAMPSASRSRRREIIQRFEN
VDIRLKTLPGIGELVDGKVRIEDIREVGVEDLLGRDPVPPFEDLIKSCIRDKVVLVTGAGGSIGSELCRQIILNTPKKLI
IFEQAEYALYKIEQDILKHRINFEVVPVLGDVLNTEHLEKIIASHGVQTIYHAAAYKHVPLVESNVIAGIVNNVFGTLSA
SKAAIKCKVETFVLISTDKAVRPTNIMGASKRLAELVLQGLSQDKSHSTRFCMVRFGNVLGSSGSVVPLFKEQIRHGGPV
TVTHPDITRYFMTIPEAAQLVLQAGAMGKGGDVFVLDMGESVKIVDLAKKMIELSGLEVRNPDTGQGDISIEFTGLRPGE
KLYEELLIGENVSWTAHPRIMTAEEASIELSKLMQSLDQMRSACSTGDEPAVREVLKRLVPEYKPT
>Mature_626_residues
MIHHWFYKESQMFVRIAGLPRRIKIFIMLFCDVILLPLALYSAIALRLGTIRPEVSPFWWLFLAIPFLTVPIFVRVGLYR
AVIRYMDDRIIATVFYGVSLSVLLLTAAVVMGNVTGVPRSSIVIYWIIAIAYITSSRFIARGVLRSLEREQDRRQPVAIY
GAGRSGLQTALALMSGPEFRPVAFFDDNRQLHGTSVAGIRVYSPDEALNIMGAEDCNQLLIAMPSASRSRRREIIQRFEN
VDIRLKTLPGIGELVDGKVRIEDIREVGVEDLLGRDPVPPFEDLIKSCIRDKVVLVTGAGGSIGSELCRQIILNTPKKLI
IFEQAEYALYKIEQDILKHRINFEVVPVLGDVLNTEHLEKIIASHGVQTIYHAAAYKHVPLVESNVIAGIVNNVFGTLSA
SKAAIKCKVETFVLISTDKAVRPTNIMGASKRLAELVLQGLSQDKSHSTRFCMVRFGNVLGSSGSVVPLFKEQIRHGGPV
TVTHPDITRYFMTIPEAAQLVLQAGAMGKGGDVFVLDMGESVKIVDLAKKMIELSGLEVRNPDTGQGDISIEFTGLRPGE
KLYEELLIGENVSWTAHPRIMTAEEASIELSKLMQSLDQMRSACSTGDEPAVREVLKRLVPEYKPT

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=262, Percent_Identity=24.4274809160305, Blast_Score=77, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 69452; Mature: 69452

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHHWFYKESQMFVRIAGLPRRIKIFIMLFCDVILLPLALYSAIALRLGTIRPEVSPFWW
CCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LFLAIPFLTVPIFVRVGLYRAVIRYMDDRIIATVFYGVSLSVLLLTAAVVMGNVTGVPRS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SIVIYWIIAIAYITSSRFIARGVLRSLEREQDRRQPVAIYGAGRSGLQTALALMSGPEFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCC
PVAFFDDNRQLHGTSVAGIRVYSPDEALNIMGAEDCNQLLIAMPSASRSRRREIIQRFEN
EEEEECCCCEECCCEEEEEEEECCCHHHEECCHHCCCCEEEECCCCCHHHHHHHHHHHCC
VDIRLKTLPGIGELVDGKVRIEDIREVGVEDLLGRDPVPPFEDLIKSCIRDKVVLVTGAG
CCEEEEECCCCCHHHCCCEEEHHHHHCCHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCC
GSIGSELCRQIILNTPKKLIIFEQAEYALYKIEQDILKHRINFEVVPVLGDVLNTEHLEK
CCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCEEEEEHHHHHHCHHHHHH
IIASHGVQTIYHAAAYKHVPLVESNVIAGIVNNVFGTLSASKAAIKCKVETFVLISTDKA
HHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCC
VRPTNIMGASKRLAELVLQGLSQDKSHSTRFCMVRFGNVLGSSGSVVPLFKEQIRHGGPV
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHCCCCCEEHHHHHHHHCCCCE
TVTHPDITRYFMTIPEAAQLVLQAGAMGKGGDVFVLDMGESVKIVDLAKKMIELSGLEVR
EEECCHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEC
NPDTGQGDISIEFTGLRPGEKLYEELLIGENVSWTAHPRIMTAEEASIELSKLMQSLDQM
CCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCEECCCCEEECCHHHHHHHHHHHHHHHH
RSACSTGDEPAVREVLKRLVPEYKPT
HHHHCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIHHWFYKESQMFVRIAGLPRRIKIFIMLFCDVILLPLALYSAIALRLGTIRPEVSPFWW
CCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LFLAIPFLTVPIFVRVGLYRAVIRYMDDRIIATVFYGVSLSVLLLTAAVVMGNVTGVPRS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SIVIYWIIAIAYITSSRFIARGVLRSLEREQDRRQPVAIYGAGRSGLQTALALMSGPEFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCC
PVAFFDDNRQLHGTSVAGIRVYSPDEALNIMGAEDCNQLLIAMPSASRSRRREIIQRFEN
EEEEECCCCEECCCEEEEEEEECCCHHHEECCHHCCCCEEEECCCCCHHHHHHHHHHHCC
VDIRLKTLPGIGELVDGKVRIEDIREVGVEDLLGRDPVPPFEDLIKSCIRDKVVLVTGAG
CCEEEEECCCCCHHHCCCEEEHHHHHCCHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCC
GSIGSELCRQIILNTPKKLIIFEQAEYALYKIEQDILKHRINFEVVPVLGDVLNTEHLEK
CCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCEEEEEHHHHHHCHHHHHH
IIASHGVQTIYHAAAYKHVPLVESNVIAGIVNNVFGTLSASKAAIKCKVETFVLISTDKA
HHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCC
VRPTNIMGASKRLAELVLQGLSQDKSHSTRFCMVRFGNVLGSSGSVVPLFKEQIRHGGPV
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHCCCCCEEHHHHHHHHCCCCE
TVTHPDITRYFMTIPEAAQLVLQAGAMGKGGDVFVLDMGESVKIVDLAKKMIELSGLEVR
EEECCHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEC
NPDTGQGDISIEFTGLRPGEKLYEELLIGENVSWTAHPRIMTAEEASIELSKLMQSLDQM
CCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCEECCCCEEECCHHHHHHHHHHHHHHHH
RSACSTGDEPAVREVLKRLVPEYKPT
HHHHCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]