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The map label for this gene is pilS [H]

Identifier: 42523019

GI number: 42523019

Start: 1433931

End: 1435508

Strand: Direct

Name: pilS [H]

Synonym: Bd1512

Alternate gene names: 42523019

Gene position: 1433931-1435508 (Clockwise)

Preceding gene: 42523018

Following gene: 42523020

Centisome position: 37.91

GC content: 51.77

Gene sequence:

>1578_bases
ATGCGTTTAAGTTTTGCTTTGCAGAACAGTAAAAATCAGGGGCTGACGGTGGAATTTACCCGCGTCAGCCTTTTTGCGTT
GATTTTGCTGATCAGTGCGGTTTCCAGTGTGTTTCAGGAAGGTTTCATTAACTGGCAGATTCTGGGACCGTTTTACGCGA
TCCTGAGTGTGGCCTTTGGCGCGCATGTGGTGTGGTTCTCGATGTGGGACCGCCTGCTGAAAAGTCCGTGGCTGTTGTTC
GCGGGCTTCGTCGTTGACTCCCTGCTGATTTCTTTTTTGATCTATTACTCCGGAATTAATCAGTCGCTGTTTTTGTTCCT
GCACCTGGTGAATATCCTGCTGGCGGGGATTGCCTGCCGGGGTGTGGGGGCGGTGACACTGGCCCTGTTCACAAGCATCT
TCTTTACGGTGGCGGCGCTGTTCTCGCCAGAGATGAAGGCGTTGAATTTCTTCTTCCTGCTGGCTTTGAACAACATTGCG
TTCTTCTCGGTGGCGGGTCTTTCCGGGTACCTGAGTGAGCAGCTGCAAACAGTCGGGAAAGAGCTTTCGGAAACAGGCGC
CAGCCTTCGTTCGGCTCAGGAGCTGAACGAGGTTCTGGTTGAAAACATCCCGACCGGGATGGTGTCTTTCACTGAATCCG
GCGAAGTGGTGAAAGCCAATTCATCGGCGGCCGAGATCCTGGGGTATTCCGATCTTTCCACTTTGAACTGGTACGAACTG
TTCCCGGAAACCCGCAGGTCTGAAGGATTGTTCAAAGGGGATGTGAAGTACACTCCTCAAAATGAAGCCAACGCCAAGAT
TCTGGGTATGACCTTGTCAAAGATCTATAGTCCCGAGTTGAAAGCGCATCTGTCTATTGGCTTGTTTGATGACCTGACCA
AGATTCGCCAGCTTGAATATGCTGCCAGACAAAATGAAAAGCTGGCGGCCGTTGGTGGTTTGGCCGCGGGGATTGCCCAT
GAAATTCGCAACCCGCTGGCGGGTATCAGTGGCAGTATCGAGCTTTTGACCCAGACGGTGAACAACGACGATGACCGCAA
ACTGATGAAAATCGTTTTGCGTGAAATTGACCGCTTGAACAATTTGATCACCGAATTCCTGGATTATGCCCGTCCGGAAG
TGCCGCCAACAGATGCGGTGGACCTTTCTTCGCTGCTTACGGAAGTGCTGGATTCGATGAAACTCAATGCCCAGGTTCGC
GCCGATGTTGAACAGGTGCGCGAGTACGAGGCTGGGTTGAAGATCCTGGGCCGCCGGGACAAATTGAAACAGGCATTCCT
GAACATCATCATCAATTCCTATCAGGCTATGAATGATTCTCAGAAACCGCAGATTGCGGTGAAGGCTCTGGTCACTGACA
AAGAGGTGCGTGTGCGCATTCGTGATGCCGGGTGTGGCATGAGTGAGGCGACACGCAAGAAGATGTTTGAACCTTTCCAT
ACGACAAAGCCCAAAGGCACAGGCCTTGGTCTAGCTGTGACTCATAAGATTTTAGAGGGACACGGGGCGCAGGTGTTCGT
GGAGAGTGAAGTGGGCGTAGGTACTGAGTTTATTTTGACTTTCCCCAGAGCACACTGA

Upstream 100 bases:

>100_bases
GCTGGAGCCATTATTGATGGTTGTTCTGGGTGGTATCATCGCGGTTCTGGTTGTGGCGATGTATCTTCCGATCTTCAACA
TGGCGGATGTGGTTCAATAG

Downstream 100 bases:

>100_bases
GAGAATAGTTTTTAACACAAGGACGTGGGAGCACATAAATGAAGTCGAGAATTCTTGTTGTCGATGACGAAGAATCAATA
CGCGAGTTTTTAGAGATCAT

Product: pilS sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MRLSFALQNSKNQGLTVEFTRVSLFALILLISAVSSVFQEGFINWQILGPFYAILSVAFGAHVVWFSMWDRLLKSPWLLF
AGFVVDSLLISFLIYYSGINQSLFLFLHLVNILLAGIACRGVGAVTLALFTSIFFTVAALFSPEMKALNFFFLLALNNIA
FFSVAGLSGYLSEQLQTVGKELSETGASLRSAQELNEVLVENIPTGMVSFTESGEVVKANSSAAEILGYSDLSTLNWYEL
FPETRRSEGLFKGDVKYTPQNEANAKILGMTLSKIYSPELKAHLSIGLFDDLTKIRQLEYAARQNEKLAAVGGLAAGIAH
EIRNPLAGISGSIELLTQTVNNDDDRKLMKIVLREIDRLNNLITEFLDYARPEVPPTDAVDLSSLLTEVLDSMKLNAQVR
ADVEQVREYEAGLKILGRRDKLKQAFLNIIINSYQAMNDSQKPQIAVKALVTDKEVRVRIRDAGCGMSEATRKKMFEPFH
TTKPKGTGLGLAVTHKILEGHGAQVFVESEVGVGTEFILTFPRAH

Sequences:

>Translated_525_residues
MRLSFALQNSKNQGLTVEFTRVSLFALILLISAVSSVFQEGFINWQILGPFYAILSVAFGAHVVWFSMWDRLLKSPWLLF
AGFVVDSLLISFLIYYSGINQSLFLFLHLVNILLAGIACRGVGAVTLALFTSIFFTVAALFSPEMKALNFFFLLALNNIA
FFSVAGLSGYLSEQLQTVGKELSETGASLRSAQELNEVLVENIPTGMVSFTESGEVVKANSSAAEILGYSDLSTLNWYEL
FPETRRSEGLFKGDVKYTPQNEANAKILGMTLSKIYSPELKAHLSIGLFDDLTKIRQLEYAARQNEKLAAVGGLAAGIAH
EIRNPLAGISGSIELLTQTVNNDDDRKLMKIVLREIDRLNNLITEFLDYARPEVPPTDAVDLSSLLTEVLDSMKLNAQVR
ADVEQVREYEAGLKILGRRDKLKQAFLNIIINSYQAMNDSQKPQIAVKALVTDKEVRVRIRDAGCGMSEATRKKMFEPFH
TTKPKGTGLGLAVTHKILEGHGAQVFVESEVGVGTEFILTFPRAH
>Mature_525_residues
MRLSFALQNSKNQGLTVEFTRVSLFALILLISAVSSVFQEGFINWQILGPFYAILSVAFGAHVVWFSMWDRLLKSPWLLF
AGFVVDSLLISFLIYYSGINQSLFLFLHLVNILLAGIACRGVGAVTLALFTSIFFTVAALFSPEMKALNFFFLLALNNIA
FFSVAGLSGYLSEQLQTVGKELSETGASLRSAQELNEVLVENIPTGMVSFTESGEVVKANSSAAEILGYSDLSTLNWYEL
FPETRRSEGLFKGDVKYTPQNEANAKILGMTLSKIYSPELKAHLSIGLFDDLTKIRQLEYAARQNEKLAAVGGLAAGIAH
EIRNPLAGISGSIELLTQTVNNDDDRKLMKIVLREIDRLNNLITEFLDYARPEVPPTDAVDLSSLLTEVLDSMKLNAQVR
ADVEQVREYEAGLKILGRRDKLKQAFLNIIINSYQAMNDSQKPQIAVKALVTDKEVRVRIRDAGCGMSEATRKKMFEPFH
TTKPKGTGLGLAVTHKILEGHGAQVFVESEVGVGTEFILTFPRAH

Specific function: Member of the two-component regulatory system AtoS/AtoC; may activate AtoC by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=363, Percent_Identity=30.8539944903581, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1790436, Length=226, Percent_Identity=34.9557522123894, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1790300, Length=237, Percent_Identity=27.0042194092827, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1786600, Length=242, Percent_Identity=25.6198347107438, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789808, Length=193, Percent_Identity=25.9067357512953, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI48994928, Length=260, Percent_Identity=26.9230769230769, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI145693157, Length=225, Percent_Identity=25.7777777777778, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789149, Length=248, Percent_Identity=25.4032258064516, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI87081816, Length=216, Percent_Identity=25, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786783, Length=219, Percent_Identity=23.7442922374429, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57996; Mature: 57996

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSFALQNSKNQGLTVEFTRVSLFALILLISAVSSVFQEGFINWQILGPFYAILSVAFG
CCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHH
AHVVWFSMWDRLLKSPWLLFAGFVVDSLLISFLIYYSGINQSLFLFLHLVNILLAGIACR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
GVGAVTLALFTSIFFTVAALFSPEMKALNFFFLLALNNIAFFSVAGLSGYLSEQLQTVGK
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
ELSETGASLRSAQELNEVLVENIPTGMVSFTESGEVVKANSSAAEILGYSDLSTLNWYEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEECCCCHHHHHCCCCCCCCHHHHH
FPETRRSEGLFKGDVKYTPQNEANAKILGMTLSKIYSPELKAHLSIGLFDDLTKIRQLEY
CCHHHCCCCCEECCCEECCCCCCCCEEEHHHHHHHCCCCHHHHEEECHHHHHHHHHHHHH
AARQNEKLAAVGGLAAGIAHEIRNPLAGISGSIELLTQTVNNDDDRKLMKIVLREIDRLN
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NLITEFLDYARPEVPPTDAVDLSSLLTEVLDSMKLNAQVRADVEQVREYEAGLKILGRRD
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHH
KLKQAFLNIIINSYQAMNDSQKPQIAVKALVTDKEVRVRIRDAGCGMSEATRKKMFEPFH
HHHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCHHHEEEEECCCCCCHHHHHHHHCCCCC
TTKPKGTGLGLAVTHKILEGHGAQVFVESEVGVGTEFILTFPRAH
CCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCC
>Mature Secondary Structure
MRLSFALQNSKNQGLTVEFTRVSLFALILLISAVSSVFQEGFINWQILGPFYAILSVAFG
CCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHH
AHVVWFSMWDRLLKSPWLLFAGFVVDSLLISFLIYYSGINQSLFLFLHLVNILLAGIACR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
GVGAVTLALFTSIFFTVAALFSPEMKALNFFFLLALNNIAFFSVAGLSGYLSEQLQTVGK
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
ELSETGASLRSAQELNEVLVENIPTGMVSFTESGEVVKANSSAAEILGYSDLSTLNWYEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEECCCCHHHHHCCCCCCCCHHHHH
FPETRRSEGLFKGDVKYTPQNEANAKILGMTLSKIYSPELKAHLSIGLFDDLTKIRQLEY
CCHHHCCCCCEECCCEECCCCCCCCEEEHHHHHHHCCCCHHHHEEECHHHHHHHHHHHHH
AARQNEKLAAVGGLAAGIAHEIRNPLAGISGSIELLTQTVNNDDDRKLMKIVLREIDRLN
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NLITEFLDYARPEVPPTDAVDLSSLLTEVLDSMKLNAQVRADVEQVREYEAGLKILGRRD
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHH
KLKQAFLNIIINSYQAMNDSQKPQIAVKALVTDKEVRVRIRDAGCGMSEATRKKMFEPFH
HHHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCHHHEEEEECCCCCCHHHHHHHHCCCCC
TTKPKGTGLGLAVTHKILEGHGAQVFVESEVGVGTEFILTFPRAH
CCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8346225; 9097040; 9278503 [H]