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The map label for this gene is pilR [H]

Identifier: 42523020

GI number: 42523020

Start: 1435547

End: 1436953

Strand: Direct

Name: pilR [H]

Synonym: Bd1513

Alternate gene names: 42523020

Gene position: 1435547-1436953 (Clockwise)

Preceding gene: 42523019

Following gene: 42523021

Centisome position: 37.95

GC content: 48.47

Gene sequence:

>1407_bases
ATGAAGTCGAGAATTCTTGTTGTCGATGACGAAGAATCAATACGCGAGTTTTTAGAGATCATGCTGAAAAAAGAAGGGTA
TGAAATCACTTTGGCTGAAGACGGCCAAAAGGCGAAAGACCTTCTTGCGAAAAAAACATTTGATATGATCATCTCAGACT
TGCAGATGCCCCATGTGACGGGGATCGAGCTTCTGAAGCACGTCAAAGAATCCTATCCGGATACAGTCTTCATGCTGATC
ACAGCGTTCGGTACAACCGAAACTGCCGTGGAAGCGATGAAGATGGGTGCTTATGATTATCTGACAAAACCATTCAAAAT
TGATGAAGTCCGTCTGAACATCCAGAACGCACTTCGTTCCCGCAACTTGGAAGTGGAAAACAGATCCTTGAAAAAGGAAC
TGGTAAAAGAATACTCCTTCCAGAACATGGTGGGGAATTCCCCGGTCATGCATGCGATCTACGATATGGTCAAGCGTGTC
TCCCAGACGCCAACCAACGTTTTGATCACAGGGGAATCCGGTACGGGTAAAGAGGTTGTGGCCAAGGCGATTCACTATAA
CGGTCCGTTGAAAGACCGTCCATTTGTAACAGTGAACTGCGGTGCCATCCCTGAGAACCTGATGGAATCCGAAATGTTCG
GTCACAAAAAAGGCTCTTTCACCGGTGCGGTGGCTGATAAAGCGGGTCTTTTTGAAGTGGCTGACGGCGGAACGCTGTTC
CTGGATGAGGTCGGTGAATTGCCATTGACGATTCAGGTAAAGCTTCTTCGTGCGATTCAAGAGCGTGTGATCCGCCGCGT
GGGTGCGACGGAAGACATGAAGGTGGATGTGCGTATCATCGCCGCGACCAACCGTAATTTGGAAGAGATGGTTCAAAAGG
GCGGTTTCCGTCAGGATTTGTTCTATCGTCTGAATGTTATCAATATCAAAACGCCGGGTCTTCGTGAGCGTCGTGATGAT
ATCCCGCTTTTGGCAAATCACTTCCTGAAAAAGTACAACGAACGTCTGAACAAAAACATCGGTGCGATCAGCGCGGATGC
GATGGAAATCCTGAAAAAGTACGACTATCCAGGGAACGTGCGTGAGCTTGAAAACATGATCGAGCGCACCGTGGCTTTGG
AAGGCGGGGCGACGATTCTTCCGGAATCTTTGCCACCGATGGTGAACACCTCTTCCGGTCGCAAGATGGCTTCCTCCAAT
GAAATCGAAATCGGTGACGATGGGGTTGATTTGGACAAGGTGATGGGTCAGATCGAAAAAGAACTTCTGATCAAAGCCAT
CCACTCTGCCGGCGGCGTGAAAAAGAGAGCTGCAAAGCTGCTTCATATCTCCTTCAGATCCATGCGATATCGCATTGAAA
AGTATAATCTAGGCGTAGTCGGCGACGACGAGCTCGACGACGAATAA

Upstream 100 bases:

>100_bases
TCGTGGAGAGTGAAGTGGGCGTAGGTACTGAGTTTATTTTGACTTTCCCCAGAGCACACTGAGAGAATAGTTTTTAACAC
AAGGACGTGGGAGCACATAA

Downstream 100 bases:

>100_bases
AAGGTGCCTGGTTGTTTTTTCTGGCTATACAGTGTCAAACGAGAGGGAAGCGAAAGCTTCCCTCTTTTCATTCGAGAGGC
CGTCGTTAAAGGTCTTGTTT

Product: regulator protein pilR

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MKSRILVVDDEESIREFLEIMLKKEGYEITLAEDGQKAKDLLAKKTFDMIISDLQMPHVTGIELLKHVKESYPDTVFMLI
TAFGTTETAVEAMKMGAYDYLTKPFKIDEVRLNIQNALRSRNLEVENRSLKKELVKEYSFQNMVGNSPVMHAIYDMVKRV
SQTPTNVLITGESGTGKEVVAKAIHYNGPLKDRPFVTVNCGAIPENLMESEMFGHKKGSFTGAVADKAGLFEVADGGTLF
LDEVGELPLTIQVKLLRAIQERVIRRVGATEDMKVDVRIIAATNRNLEEMVQKGGFRQDLFYRLNVINIKTPGLRERRDD
IPLLANHFLKKYNERLNKNIGAISADAMEILKKYDYPGNVRELENMIERTVALEGGATILPESLPPMVNTSSGRKMASSN
EIEIGDDGVDLDKVMGQIEKELLIKAIHSAGGVKKRAAKLLHISFRSMRYRIEKYNLGVVGDDELDDE

Sequences:

>Translated_468_residues
MKSRILVVDDEESIREFLEIMLKKEGYEITLAEDGQKAKDLLAKKTFDMIISDLQMPHVTGIELLKHVKESYPDTVFMLI
TAFGTTETAVEAMKMGAYDYLTKPFKIDEVRLNIQNALRSRNLEVENRSLKKELVKEYSFQNMVGNSPVMHAIYDMVKRV
SQTPTNVLITGESGTGKEVVAKAIHYNGPLKDRPFVTVNCGAIPENLMESEMFGHKKGSFTGAVADKAGLFEVADGGTLF
LDEVGELPLTIQVKLLRAIQERVIRRVGATEDMKVDVRIIAATNRNLEEMVQKGGFRQDLFYRLNVINIKTPGLRERRDD
IPLLANHFLKKYNERLNKNIGAISADAMEILKKYDYPGNVRELENMIERTVALEGGATILPESLPPMVNTSSGRKMASSN
EIEIGDDGVDLDKVMGQIEKELLIKAIHSAGGVKKRAAKLLHISFRSMRYRIEKYNLGVVGDDELDDE
>Mature_468_residues
MKSRILVVDDEESIREFLEIMLKKEGYEITLAEDGQKAKDLLAKKTFDMIISDLQMPHVTGIELLKHVKESYPDTVFMLI
TAFGTTETAVEAMKMGAYDYLTKPFKIDEVRLNIQNALRSRNLEVENRSLKKELVKEYSFQNMVGNSPVMHAIYDMVKRV
SQTPTNVLITGESGTGKEVVAKAIHYNGPLKDRPFVTVNCGAIPENLMESEMFGHKKGSFTGAVADKAGLFEVADGGTLF
LDEVGELPLTIQVKLLRAIQERVIRRVGATEDMKVDVRIIAATNRNLEEMVQKGGFRQDLFYRLNVINIKTPGLRERRDD
IPLLANHFLKKYNERLNKNIGAISADAMEILKKYDYPGNVRELENMIERTVALEGGATILPESLPPMVNTSSGRKMASSN
EIEIGDDGVDLDKVMGQIEKELLIKAIHSAGGVKKRAAKLLHISFRSMRYRIEKYNLGVVGDDELDDE

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=456, Percent_Identity=42.9824561403509, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1790437, Length=451, Percent_Identity=42.350332594235, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1790299, Length=451, Percent_Identity=39.689578713969, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1788905, Length=377, Percent_Identity=42.1750663129974, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI87082117, Length=280, Percent_Identity=48.9285714285714, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1789233, Length=327, Percent_Identity=43.7308868501529, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI1789087, Length=264, Percent_Identity=48.4848484848485, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI87082152, Length=232, Percent_Identity=51.2931034482759, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1786524, Length=381, Percent_Identity=37.007874015748, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI87081872, Length=319, Percent_Identity=36.6771159874608, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1787583, Length=329, Percent_Identity=34.0425531914894, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1789828, Length=289, Percent_Identity=30.4498269896194, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI87081858, Length=320, Percent_Identity=27.1875, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=38.0165289256198, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=25.2252252252252, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787375, Length=138, Percent_Identity=29.7101449275362, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787487, Length=103, Percent_Identity=33.9805825242718, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789402, Length=124, Percent_Identity=29.0322580645161, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1786911, Length=120, Percent_Identity=31.6666666666667, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789809, Length=132, Percent_Identity=26.5151515151515, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790863, Length=136, Percent_Identity=28.6764705882353, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=28.8135593220339, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52559; Mature: 52559

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRILVVDDEESIREFLEIMLKKEGYEITLAEDGQKAKDLLAKKTFDMIISDLQMPHVT
CCCCEEEECCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHH
GIELLKHVKESYPDTVFMLITAFGTTETAVEAMKMGAYDYLTKPFKIDEVRLNIQNALRS
HHHHHHHHHHHCCCEEEEEEHHCCCHHHHHHHHHHCCHHHHCCCCEEHHHHEHHHHHHHH
RNLEVENRSLKKELVKEYSFQNMVGNSPVMHAIYDMVKRVSQTPTNVLITGESGTGKEVV
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
AKAIHYNGPLKDRPFVTVNCGAIPENLMESEMFGHKKGSFTGAVADKAGLFEVADGGTLF
HHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEECCCCCCCEEECCCCEEE
LDEVGELPLTIQVKLLRAIQERVIRRVGATEDMKVDVRIIAATNRNLEEMVQKGGFRQDL
EECCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHCCCCCCCC
FYRLNVINIKTPGLRERRDDIPLLANHFLKKYNERLNKNIGAISADAMEILKKYDYPGNV
EEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCH
RELENMIERTVALEGGATILPESLPPMVNTSSGRKMASSNEIEIGDDGVDLDKVMGQIEK
HHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
ELLIKAIHSAGGVKKRAAKLLHISFRSMRYRIEKYNLGVVGDDELDDE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCC
>Mature Secondary Structure
MKSRILVVDDEESIREFLEIMLKKEGYEITLAEDGQKAKDLLAKKTFDMIISDLQMPHVT
CCCCEEEECCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHH
GIELLKHVKESYPDTVFMLITAFGTTETAVEAMKMGAYDYLTKPFKIDEVRLNIQNALRS
HHHHHHHHHHHCCCEEEEEEHHCCCHHHHHHHHHHCCHHHHCCCCEEHHHHEHHHHHHHH
RNLEVENRSLKKELVKEYSFQNMVGNSPVMHAIYDMVKRVSQTPTNVLITGESGTGKEVV
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
AKAIHYNGPLKDRPFVTVNCGAIPENLMESEMFGHKKGSFTGAVADKAGLFEVADGGTLF
HHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEECCCCCCCEEECCCCEEE
LDEVGELPLTIQVKLLRAIQERVIRRVGATEDMKVDVRIIAATNRNLEEMVQKGGFRQDL
EECCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHCCCCCCCC
FYRLNVINIKTPGLRERRDDIPLLANHFLKKYNERLNKNIGAISADAMEILKKYDYPGNV
EEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCH
RELENMIERTVALEGGATILPESLPPMVNTSSGRKMASSNEIEIGDDGVDLDKVMGQIEK
HHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
ELLIKAIHSAGGVKKRAAKLLHISFRSMRYRIEKYNLGVVGDDELDDE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]