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The map label for this gene is glyS

Identifier: 42522689

GI number: 42522689

Start: 1087485

End: 1088825

Strand: Direct

Name: glyS

Synonym: Bd1147

Alternate gene names: 42522689

Gene position: 1087485-1088825 (Clockwise)

Preceding gene: 42522681

Following gene: 42522692

Centisome position: 28.75

GC content: 48.62

Gene sequence:

>1341_bases
ATGAAAATTAAGCATCTTGAGGACCTAAACACTCTTGTGAGCCTCAGCAAACGTCGTGGTTTTGTATTCCAATCCAGTGA
GATCTATGGCGGTCTTGGAAGTTGCTGGGATTACGGTCCTCTGGGCTCTTTGATGAAATTGAATGTGAAGCGTGCTTGGT
GGAATGCCATGACCCGCAGACCTGATATCGTGGGTCTGGATGCCGCGATCCTGATGCACCCGATGGTGTGGAAAGCCTCC
GGTCACGTGGATGGTTTCTCCGATCCATTGGTGGACTGTAAGGAGTGTAAAACACGCTTCCGTGCGGATAACACGGATTC
CTATATCAACGAAAAGAAATGCCCGAACTGCGGCAGCAAGAATCTTTCTGAAGAAAGATCCTTCAACCTGATGTTCAAAA
CTCACATGGGTCCTTTGGAAGATTCCGGCAGCGTGGTGTATCTGCGTCCGGAAACTGCTCAAGGTCACTTCGTGAACTTC
CAGAACTGTCAGCAGGCATCCCGTTATAAAATTCCATTCGGTATCGCGGCGATTGGTAAATCCTTCCGTAACGAAATCAC
ACCAGGAAACTTCATCTTCCGTACCCGTGAATTCGAACAGATGGAAATGCAATATTTCGTAGAGCCAGGCACGGACGAAC
AGTTCTTCAAAGAATGGAAAGAGCGTCGCTGGAATTTCTATATCAAGTTCGGTATCAAACCAGAGAATTTGAAATTCAAA
GATCACGACAAGCTGGCGCACTATGCGAAAGCGGCTGTGGACGTGGAATTCAAGTTCCCAATGGGCTTCTCTGAATTGGA
AGGTATCCACAATCGTTCGGACTTCGATTTGTCCCAGCACATGAAATTCTCCGGTAAAAACCTGGAGTACTTCGACGAAC
CGAACAAAAAGAAATACATCCCTTATGTGATTGAAACAGCCGTGGGTTGTGATCGCCTGTTCCTGGCGTTCCTGTGTGAT
GCTTACCGTGAGGAAGTGACGACAGACGAAGCGGGTAAAGAAGACGTTCGCGTGGTGATGGGTCTGCACCCGGAAATCGC
TCCGTTCAAAGTGGCAGTTCTGCCTCTGTCCAAAAAAGAGGAGCTAAGCTCTATCTCTGAAAAACTGCGTGACCAATTGG
CTGAAGACTTCGACGTGAACTATGACGAATCCCAGTCCATCGGTAAACGTTACCGTCGTCAGGACGAGATCGGCACACCG
TTCTGCGTGACTGTGGACTTTGACACCATCAATGATCAGGCGGTGACCGTTCGTCACCGTGACAAGATGACTCAAGAGCG
CGTGGCGATCACTCAGTTGAACGCATACATCGCAGCCAAGTTGAAGACCTTTAATCAGTAG

Upstream 100 bases:

>100_bases
CCTCAGGCAAGGCAAGACCCTGATTTCTTGACTGGTTCTGGACCCAAGTGTAACGATAAGCACCGGGACTTTGTCCCTTT
TTCAAAACTTGGAGCCCTGC

Downstream 100 bases:

>100_bases
CCCGACAGGGGCAGAGCTGAAGCTGCTTTAAATTAAAAAGGGAGTGCATCGCACTCCCTTTTTTTATTTCCATGATATCA
CAAGCTCGGTGAGCTTATTT

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKIKHLEDLNTLVSLSKRRGFVFQSSEIYGGLGSCWDYGPLGSLMKLNVKRAWWNAMTRRPDIVGLDAAILMHPMVWKAS
GHVDGFSDPLVDCKECKTRFRADNTDSYINEKKCPNCGSKNLSEERSFNLMFKTHMGPLEDSGSVVYLRPETAQGHFVNF
QNCQQASRYKIPFGIAAIGKSFRNEITPGNFIFRTREFEQMEMQYFVEPGTDEQFFKEWKERRWNFYIKFGIKPENLKFK
DHDKLAHYAKAAVDVEFKFPMGFSELEGIHNRSDFDLSQHMKFSGKNLEYFDEPNKKKYIPYVIETAVGCDRLFLAFLCD
AYREEVTTDEAGKEDVRVVMGLHPEIAPFKVAVLPLSKKEELSSISEKLRDQLAEDFDVNYDESQSIGKRYRRQDEIGTP
FCVTVDFDTINDQAVTVRHRDKMTQERVAITQLNAYIAAKLKTFNQ

Sequences:

>Translated_446_residues
MKIKHLEDLNTLVSLSKRRGFVFQSSEIYGGLGSCWDYGPLGSLMKLNVKRAWWNAMTRRPDIVGLDAAILMHPMVWKAS
GHVDGFSDPLVDCKECKTRFRADNTDSYINEKKCPNCGSKNLSEERSFNLMFKTHMGPLEDSGSVVYLRPETAQGHFVNF
QNCQQASRYKIPFGIAAIGKSFRNEITPGNFIFRTREFEQMEMQYFVEPGTDEQFFKEWKERRWNFYIKFGIKPENLKFK
DHDKLAHYAKAAVDVEFKFPMGFSELEGIHNRSDFDLSQHMKFSGKNLEYFDEPNKKKYIPYVIETAVGCDRLFLAFLCD
AYREEVTTDEAGKEDVRVVMGLHPEIAPFKVAVLPLSKKEELSSISEKLRDQLAEDFDVNYDESQSIGKRYRRQDEIGTP
FCVTVDFDTINDQAVTVRHRDKMTQERVAITQLNAYIAAKLKTFNQ
>Mature_446_residues
MKIKHLEDLNTLVSLSKRRGFVFQSSEIYGGLGSCWDYGPLGSLMKLNVKRAWWNAMTRRPDIVGLDAAILMHPMVWKAS
GHVDGFSDPLVDCKECKTRFRADNTDSYINEKKCPNCGSKNLSEERSFNLMFKTHMGPLEDSGSVVYLRPETAQGHFVNF
QNCQQASRYKIPFGIAAIGKSFRNEITPGNFIFRTREFEQMEMQYFVEPGTDEQFFKEWKERRWNFYIKFGIKPENLKFK
DHDKLAHYAKAAVDVEFKFPMGFSELEGIHNRSDFDLSQHMKFSGKNLEYFDEPNKKKYIPYVIETAVGCDRLFLAFLCD
AYREEVTTDEAGKEDVRVVMGLHPEIAPFKVAVLPLSKKEELSSISEKLRDQLAEDFDVNYDESQSIGKRYRRQDEIGTP
FCVTVDFDTINDQAVTVRHRDKMTQERVAITQLNAYIAAKLKTFNQ

Specific function: Catalyzes the attachment of glycine to tRNA(Gly)

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI116805340, Length=352, Percent_Identity=32.3863636363636, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI70887790, Length=445, Percent_Identity=24.2696629213483, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI32564541, Length=360, Percent_Identity=30.5555555555556, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17554996, Length=360, Percent_Identity=30.5555555555556, Blast_Score=144, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6325338, Length=385, Percent_Identity=28.8311688311688, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319597, Length=252, Percent_Identity=32.5396825396825, Blast_Score=112, Evalue=9e-26,
Organism=Drosophila melanogaster, GI21357965, Length=358, Percent_Identity=31.2849162011173, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24664462, Length=358, Percent_Identity=31.2849162011173, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYG_BDEBA (Q6MNT9)

Other databases:

- EMBL:   BX842649
- RefSeq:   NP_968069.1
- ProteinModelPortal:   Q6MNT9
- SMR:   Q6MNT9
- GeneID:   2735034
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd1147
- NMPDR:   fig|264462.1.peg.1039
- HOGENOM:   HBG319978
- OMA:   GSEWGEL
- PhylomeDB:   Q6MNT9
- ProtClustDB:   PRK04173
- BioCyc:   BBAC264462:BD1147-MONOMER
- BRENDA:   6.1.1.14
- GO:   GO:0005737
- HAMAP:   MF_00253_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022961
- InterPro:   IPR002315
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR10745
- PRINTS:   PR01043
- TIGRFAMs:   TIGR00389

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.14

Molecular weight: Translated: 51595; Mature: 51595

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS50862 AA_TRNA_LIGASE_II; PS00307 LECTIN_LEGUME_BETA

Important sites: BINDING 101-101 BINDING 151-151

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKHLEDLNTLVSLSKRRGFVFQSSEIYGGLGSCWDYGPLGSLMKLNVKRAWWNAMTRR
CCCCHHHHHHHHHHHHHHCCEEEECCHHCCCCCCCCCCCCCCHHHEEHHHHHHHHHHHCC
PDIVGLDAAILMHPMVWKASGHVDGFSDPLVDCKECKTRFRADNTDSYINEKKCPNCGSK
CCEECCCHHHHHCCHHEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCC
NLSEERSFNLMFKTHMGPLEDSGSVVYLRPETAQGHFVNFQNCQQASRYKIPFGIAAIGK
CCCCHHCEEEEEEECCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHCCCCCCCHHHHHHH
SFRNEITPGNFIFRTREFEQMEMQYFVEPGTDEQFFKEWKERRWNFYIKFGIKPENLKFK
HHHHCCCCCCEEEECCHHHHHCCCEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCC
DHDKLAHYAKAAVDVEFKFPMGFSELEGIHNRSDFDLSQHMKFSGKNLEYFDEPNKKKYI
CHHHHHHHHHHHEEEEEECCCCHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCC
PYVIETAVGCDRLFLAFLCDAYREEVTTDEAGKEDVRVVMGLHPEIAPFKVAVLPLSKKE
CCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCHH
ELSSISEKLRDQLAEDFDVNYDESQSIGKRYRRQDEIGTPFCVTVDFDTINDQAVTVRHR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEHH
DKMTQERVAITQLNAYIAAKLKTFNQ
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIKHLEDLNTLVSLSKRRGFVFQSSEIYGGLGSCWDYGPLGSLMKLNVKRAWWNAMTRR
CCCCHHHHHHHHHHHHHHCCEEEECCHHCCCCCCCCCCCCCCHHHEEHHHHHHHHHHHCC
PDIVGLDAAILMHPMVWKASGHVDGFSDPLVDCKECKTRFRADNTDSYINEKKCPNCGSK
CCEECCCHHHHHCCHHEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCC
NLSEERSFNLMFKTHMGPLEDSGSVVYLRPETAQGHFVNFQNCQQASRYKIPFGIAAIGK
CCCCHHCEEEEEEECCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHCCCCCCCHHHHHHH
SFRNEITPGNFIFRTREFEQMEMQYFVEPGTDEQFFKEWKERRWNFYIKFGIKPENLKFK
HHHHCCCCCCEEEECCHHHHHCCCEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCC
DHDKLAHYAKAAVDVEFKFPMGFSELEGIHNRSDFDLSQHMKFSGKNLEYFDEPNKKKYI
CHHHHHHHHHHHEEEEEECCCCHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCC
PYVIETAVGCDRLFLAFLCDAYREEVTTDEAGKEDVRVVMGLHPEIAPFKVAVLPLSKKE
CCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCHH
ELSSISEKLRDQLAEDFDVNYDESQSIGKRYRRQDEIGTPFCVTVDFDTINDQAVTVRHR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEHH
DKMTQERVAITQLNAYIAAKLKTFNQ
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA